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Establishment of enzyme-linked immunosorbent assay for beef and lamb contents in cooked meat

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未来食品学报(英文) 2024年第1期4卷 91-96页

作者： Yujing Li Jingjing liu Sufang Fan Li Zhao Jing Zhang Erjing Zhang ziran Li Yan Zhang Chunsheng Li Hebei Jiaotong Vocational and Technical College Shijiazhuang 050035China Biology Institute of Hebei Academy of Sciences Shijiazhuang 050081China Hebei Food Safety Key Labratory Key Laboratory of Special Food Supenvision Technolog for State Market RegpulationHebei Engineering Research Center for Special Food Sagfery and HealthHebei Food Inspection cand Research InstinuteShijiazhuang 050227China College ofLife Sciences Hebei Normal UniversinyShijiazhuang 050024China Julu County Applied Technology Research Instinute Jultu 055250China

In this study,an enzyme 1linked immunosorbent assay(ELISA)was established to detect beef and 1amb components,and its performance was tested.Double-antibody sandwich ELISA was adopted and determined a coating concentration of capture antibody 3G5 of 1:4000,a working concentration of enzyme-labeled antibody 2E7-horseradish peroxidase(HRP)of 1:1000,a sample incubation time of 60 min and a detection antibody reaction time of 60 min.The specificity,sensitivity,repeatability and stability of this assay were detemmined.The limit of detection for beef and 1amb skeleta1 muscle troponin I was 45 mg/kg,the inter-assay and intra-assay recovery rates ranged from 80.4%to 115.7%,the coefficients of variation were below 13.6%,and the cIoss reaction rates of the tissue components of chicken,duck and fish were below 13.4%.The sandwich ELISA method established in this study is stable and has high accuracy.The test results were consistent with the polymerase chain reaction(PCR)method at 50 and 100 g/kg-Therefore,this ELISA method can be used to quantitatively detect beef and 1amb components in meat products.

关键词： Double antibody sandwich enzyme-1inked immunosorbent assay(ELISA) Beef components Lamb components

Genome-wide identification and spatiotemporal expression profiling of zinc finger SWIM domain-containing protein family genes

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Zoological Research 2023年第3期44卷 663-674页

作者： Imtiaz Ul Hassan Hafiz Mamoon Rehman ziran liu Liangji Zhou Muhammad Kaleem Samma Chengdong Wang Zixin Rong Xufeng Qi Dongqing Cai Hui Zhao Key Laboratory for Regenerative Medicine Ministry of EducationSchool of Biomedical SciencesFaculty of MedicineThe Chinese University of Hong KongHong Kong SARChina Centre for Agricultural Biochemistry and Biotechnology(CABB) University of Agriculture FaisalabadFaisalabad 38000Pakistan Qingdao Municipal Center for Disease Control and Prevention QingdaoShandong 266034China Key Laboratory of Regenerative Medicine of Ministry of Education Department of Developmental&Regenerative BiologyJinan UniversityGuangzhou 511436China Department of Biology and Chemistry City University of Hong KongHong Kong SARChina Department of Gene Technology School of Engineering Sciences in ChemistryBiotechnology and Health(CBH)KTH Royal Institute of Technology10691 StockholmSweden Kunming Institute of Zoology-The Chinese University of Hong Kong(KIZ-CUHK)Joint Laboratory of Bioresources and Molecular Research of Common Diseases Hong Kong SARChina Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics The Chinese University of Hong KongHong Kong SARChina

The biological function of the novel zinc-finger SWIM domain-containing protein family(ZSWIM)during embryonic development remains elusive.Here,we conducted a genome-wide analysis to explore the evolutionary processes of the ZSWIM gene family members in mice,Xenopus tropicalis,zebrafish,and humans.We identified nine putative ZSWIM genes in the human and mouse genome,eight in the Xenopus genome,and five in the zebrafish genome.Based on multiple sequence alignment,three members,ZSWIM5,ZSWIM6,and ZSWIM8,demonstrated the highest homology across all four species.Using available RNA sequencing(RNAseq)data,ZSWIM genes were found to be widely expressed across different tissues,with distinct tissuespecific properties.To identify the functions of the ZSWIM protein family during embryogenesis,we examined temporal and spatial expression patterns of zswim family genes in Xenopus embryos.Quantitative real-time polymerase chain reaction(qRT-PCR)revealed that each member had a distinct expression profile.Whole-mount in situ hybridization showed that both zswim1 and zswim3 were maternally expressed genes;zswim5 and zswim6were expressed throughout embryogenesis and displayed dynamic expression in the brain,eyes,somite,and bronchial arch at the late tailbud stages;zswim7 was detected in the eye area;zswim8 showed a dynamic expression pattern during the tailbud stages,with expression detected in the brain,eyes,and somite;zswim9 was faintly expressed throughout embryonic development.This study provides a foundation for future research to delineate the functions of ZSWIM gene members.

关键词： ZSWIM gene family Xenopus Gene expression Phylogenetic analysis Bioinformatics

LncPheDB:a genome-wide lncRNAs regulated phenotypes database in plants

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aBIOTECH 2022年第3期3卷 169-177页

作者： Danjing Lou Fei Li Jinyue Ge Weiya Fan ziran liu Yanyan Wang Jingfen Huang Meng Xing Wenlong Guo Shizhuang Wang Weihua Qiao Zhenyun Han Qian Qian Qingwen Yang Xiaoming Zheng National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing100081China College of Life Science Shenyang Normal UniversityShenyang110034China National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanya572000China State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural SciencesHangzhou310006China Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural SciencesShenzhen518120China International Rice Research Institute DAPO box 7777Metro ManilaThe Philippines

LncPheDB(https://***/)is a systematic resource of genome-wide long non-coding RNAs(lncRNAs)-phenotypes associations for multiple species.It was established to display the genome-wide lncRNA annotations,target genes prediction,variant-trait associations,gene-phenotype correlations,lncRNA-phenotype correlations,and the similar non-coding regions of the queried sequence in multiple species.LncPheDB sorted out a total of 203,391 lncRNA sequences,2000 phenotypes,and 120,271 variants of nine species(Zea mays L.,Gossypium barbadense L.,Triticum aestivum L.,Lycopersicon esculentum Mille,Oryza sativa L.,Hordeum vulgare L.,Sorghum bicolor L.,Glycine max L.,and Cucumis sativus L.).By exploring the relationship between lncRNAs and the genomic position of variants in genome-wide association analysis,a total of 68,862 lncRNAs were found to be related to the diversity of agronomic traits.More importantly,to facilitate the study of the functions of lncRNAs,we analyzed the possible target genes of lncRNAs,constructed a blast tool for performing similar fragmentation studies in all species,linked the pages of phenotypic studies related to lncRNAs that possess similar fragments and constructed their regulatory networks.In addition,LncPheDB also provides a user-friendly interface,a genome visualization platform,and multi-level and multi-modal convenient data search engine.We believe that LncPheDB plays a crucial role in mining lncRNA-related plant data.

关键词： LncRNA GWAS Phenotype SNP Plants

清金化痰益肺汤合保和丸在支气管肺炎治疗中作用及对肺功能、免疫功能、FeNO、EOS、PCT、CRP水平的影响

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中华中医药学刊 2024年第4期42卷 50-53页

作者：胡自然刘锋娟李璐六安市中医院安徽六安237006

目的清金化痰益肺汤合保和丸对支气管肺炎的应用价值以及对肺功能、免疫功能、一氧化氮(Fractional ex-haled nitric oxide,FeNO)、血清嗜酸粒细胞(Eosinophil,EOS)、降钙素原(Procalcitonin,PCT)及C反应蛋白(C-reactive protein,CRP)水平的影响。方法选取医院收治的支气管肺炎患儿100例,并将患儿随机分为对照组和观察组,每组50例。对照组均给予常规西药对症治疗,观察组在对照组方案的基础上给予清金化痰益肺汤合保和丸,两组疗程均为1周,对比两组临床治疗效果、肺功能、免疫功能、呼出气FeNO、EOS、PCT及CRP水平。结果两组患者治疗后,观察组总有效率为94.00%(47/50),显著高于对照组(76.00%,38/50)(P<0.05);对照组及观察组经治疗后一秒呼气最大容积(Forced expiratory volume in one second,FEV_(1))、用力肺活量(Forced vital capacity,FVC)及FEV_(1)/FVC水平均显著提升(P<0.05),同时观察组改善显著优于对照组(P<0.05);对照组及观察组经治疗后免疫球蛋白G(Immunoglobulin G,IgG)、免疫球蛋白A(Immunoglobulin A,IgA)、免疫球蛋白M(Immunoglobulin A,IgM)水平均较治疗前提升显著(P<0.05),同时观察组改善显著优于对照组(P<0.05);对照组及观察组经治疗后FeNO、EOS、PCT及CRP水平均较治疗前降低显著(P<0.05),同时观察组改善显著优于对照组(P<0.05)。结论清金化痰益肺汤合保和丸能显著提高小儿支气管肺炎的临床治疗效果,改善患儿的肺功能及免疫功能,减轻相关炎性反应,具有临床研究意义。

关键词：清金化痰益肺汤保和丸支气管肺炎免疫功能

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Genome-wide selection and introgression of Chinese rice varieties during breeding

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Journal of Genetics and Genomics 2022年第5期49卷 492-501页

作者： Jinyue Ge Junrui Wang Hongbo Pang Fei Li Danjing Lou Weiya Fan ziran liu Jiaqi Li Danting Li Baoxuan Nong Zongqiong Zhang Yanyan Wang Jingfen Huang Meng Xing Yamin Nie Xiaorong Xiao Fan Zhang Wensheng Wang Jianlong Xu Sung Ryul Kim Ajay Kohli Guoyou Ye Weihua Qiao Qingwen Yang Xiaoming Zheng National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Guangxi Key Laboratory for Polysaccharide Materials and Modifications School of Marine Sciences and BiotechnologyGuangxi University for NationalitiesNanningJiangsu 530006China College of Life Science Shenyang Normal UniversityShenyangJilin 110034China Liaoning Institute of Pomology YingkouLiaoning 115009China Rice Research Institute Guangxi Academy of Agricultural ScienceNanningGuangxi 530007China National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanyaHainan 572000China International Rice Research Institute DAPO box 7777MetroManilaPhilippines Crop Biotech Institute&Department of Genetic Engineering Kyung Hee UniversityYongin 446-701Republic of Korea

China is the largest rice-producing country,but the genomic landscape of rice diversity has not yet been clarified.In this study,we re-sequence 1070 rice varieties collected from China(400)and other regions in Asia(670).Among the six major rice groups(aus,indica-I,indica-II,aromatic,temperate japonica,and tropical japonica),almost all Chinese varieties belong to the indica-II or temperate japonica group.Most Chinese indica varieties belong to indica-II,which consists of two subgroups developed during different phases of rice breeding.The genomic segments underlying the differences between these subgroups span36.32 Mb.The Chinese japonica rice varieties fall into the temperate japonica group,consisting of two subgroups based on their geographical distribution.The genomic segments underlying the differences between these subgroups span 27.69 Mb.These differentiated segments in the Chinese indica varieties span 45 genes with nonsynonymous mutations that are closely related to variations in plant height and grain width.Fifty-four genes with nonsynonymous mutations are associated with the differences in heading date between the two Chinese japonica subgroups.These findings provide new insights into rice diversity in China that will facilitate the molecular breeding.

关键词： Rice Genome selection Introgression Agronomic traits Breeding

基于DEA模型的我国高校科研创新效率测度与差异分析

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当代教育论坛 2024年第1期 52-60页

作者：叶芳羽李毅唐子然刘沛林长沙学院经济与管理学院长沙410022 湖南师范大学旅游学院长沙410081

高校作为科技创新的主要推动者,准确评估中国大学的研究和创新效率对于提升其研究管理能力和推动创新型国家建设具有重要的指导意义。研究采用数据包络分析(DEA)模型,对教育部认定的32所“双一流”高校2011—2021年的科研创新效率进行... 详细信息

高校作为科技创新的主要推动者,准确评估中国大学的研究和创新效率对于提升其研究管理能力和推动创新型国家建设具有重要的指导意义。研究采用数据包络分析(DEA)模型,对教育部认定的32所“双一流”高校2011—2021年的科研创新效率进行测度。此外,还分析了其时间演化规律,并从地域和层次两个角度探讨了效率差异的来源。研究结果表明:中国大学的科研创新综合效率持续稳步增长,纯技术效率也呈上升趋势,而规模效率则呈现出先上升后下降的趋势;在地域差异方面,研究发现在样本期间,大学科研创新效率呈现出东部地区、中部地区、西部地区依次递减的格局,随着时间的推移,这些地区之间的差距没有缩小;在层次差异方面,C9联盟高校的研究和创新效率高于非C9联盟高校,而随着时间的推移,二者之间的差距逐渐缩小。

关键词：高等学校科研创新效率 DEA模型资源配置

Behavior pattern mining based on spatiotemporal trajectory multidimensional information fusion

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Chinese Journal of Aeronautics 2023年第4期36卷 387-399页

作者： Qiaowen JIANG Yu liu ziran DING Shun SUN Institute of Information Fusion Naval Aviation UniversityYantai 264001China Department of Electronic Engineering Tsinghua UniversityYantai 100083China

Trajectory data mining is widely used in military and civil applications,such as early warning and surveillance system,intelligent traffic system and so on.Through trajectory similarity measurement and clustering,target behavior patterns can be found from massive spatiotemporal trajectory data.In order to mine frequent behaviors of targets from complex historical trajectory data,a behavior pattern mining algorithm based on spatiotemporal trajectory multidimensional information fusion is proposed in this paper.Firstly,spatial–temporal Hausdorff distance is pro-posed to measure multidimensional information differences of spatiotemporal trajectories,which can distinguish the behaviors with similar location but different course and velocity.On this basis,by combining the idea of k-nearest neighbor and density peak clustering,a new trajectory clustering algorithm is proposed to mine behavior patterns from trajectory data with uneven density distribu-tion.Finally,we implement the proposed algorithm in simulated and radar measured trajectory data respectively.The experimental results show that the proposed algorithm can mine target behavior patterns from different complex application scenarios more quickly and accurately com-pared to the existing methods,which has a good application prospect in intelligent monitoring tasks.

关键词： Behavior pattern Hausdorff distance Information fusion Spatiotemporal trajectory Trajectory clustering

苹果芽变品种珍富果实品质和花青苷合成基因表达分析

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果树学报 2024年第3期41卷 426-435页

作者：汪少丽车晓志石洁陈敏张紫然陈平张新忠刘保友山东省烟台市农业科学研究院山东烟台265500 莱阳市龙旺庄街道办事处农业综合服务中心山东莱阳265200 中国农业大学北京271018

【目的】测定2001富士及其芽变品种珍富之间果实品质及花青苷合成相关基因表达水平差异,明确珍富品种特性,为其示范推广提供参考依据,也为研究果皮颜色芽变机制提供理论支撑。【方法】以芽变品种珍富及2001富士果实为试材,对其采收期及... 详细信息

【目的】测定2001富士及其芽变品种珍富之间果实品质及花青苷合成相关基因表达水平差异,明确珍富品种特性,为其示范推广提供参考依据,也为研究果皮颜色芽变机制提供理论支撑。【方法】以芽变品种珍富及2001富士果实为试材,对其采收期及贮藏14 d后果实外观、内在品质、花青苷含量和合成相关基因表达水平进行测定。【结果】与2001富士相比,芽变品种珍富果实采收日期可以提前6 d,盛花期至采收期提前6 d;采收期及贮藏14 d后,外在品质方面,珍富果皮亮度、饱和度、花青苷总量及3种不同花青苷的含量均显著高于2001富士,但二者单果质量及果形指数无显著差异;果实内在品质方面,采收期及贮藏14 d后,珍富果实的可溶性固形物、维生素C含量及固酸比都显著高于2001富士,但珍富的可滴定酸含量显著低于2001富士;二者果肉硬度、破裂力在采收期及贮藏期均无显著差异;10个花青苷合成关键结构基因及5个转录因子类调控基因都有不同程度的提高,关键结构基因MdC4H、MdANS、MdUFGT及转录因子基因MdMYB10、MdMYB11、MdERF3表达量显著提高。【结论】采收期及贮藏14 d后,红色芽变品种珍富果皮亮度L*值及果皮颜色饱和度C*值均显著高于2001富士;珍富果实可溶性固形物、维生素C含量及固酸比显著提高,可滴定酸含量显著降低;珍富果皮中花青苷总量及3种不同花青苷含量都显著提高,花青苷合成相关结构基因和转录调控基因也显著上调表达。

关键词：苹果芽变品种果实品质花青苷基因表达

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多源遥感图像融合语义分割发展现状与展望

现代雷达 2024年第2期46卷 16-24页

作者：何友刘瑜谭大宁张一鸣张财生孙顺丁自然姜乔文海军航空大学山东烟台264001 清华大学电子工程系北京100084

遥感图像的语义信息提取正成为城市规划利用、土地覆盖勘察、灾害变化检测以及海上态势感知等研究方向的关键技术之一。文中从由“单源”向“多源”发展的遥感图像智能处理需求出发,首先概述并分析了大数据时代和深度学习背景下的遥感... 详细信息

遥感图像的语义信息提取正成为城市规划利用、土地覆盖勘察、灾害变化检测以及海上态势感知等研究方向的关键技术之一。文中从由“单源”向“多源”发展的遥感图像智能处理需求出发,首先概述并分析了大数据时代和深度学习背景下的遥感图像语义分割发展现状,主要包括单一来源图像语义分割、多源遥感图像融合语义分割和多源(同质/异质)遥感图像变化检测。然后在阐述主要方法的基础上,提炼并总结了多源遥感图像语义分割的关键技术,主要有单源遥感图像快速语义分割语义信息辅助的多源遥感图像精确配准与融合、基于多源遥感图像的语义信息智能提取。最后,针对多源遥感图像在轨处理需求,概括出高分辨率多源遥感图像智能一体化信息提取所面临的技术挑战,并对未来发展趋势进行展望。

关键词：多源遥感图像语义分割图像融合智能处理