检索结果-南通市图书馆

NLRP3基因敲减对高脂高糖饮食诱导的非酒精性脂肪性肝炎小鼠模型的影响

在线全文

学校读者我要写书评

暂无评论

临床肝胆病杂志 2024年第5期40卷 952-960页

作者：黄倩王卓媛安梓铭辛鑫孙沁梅苟小军胡义扬冯琴上海中医药大学附属曙光医院肝病研究所上海201203 上海中医药大学附属曙光医院科技实验中心上海201203 上海市中医临床重点实验室上海201203 肝肾疾病病证教育部重点实验室上海20120 上海市宝山区中西医结合医院上海201900

目的探讨NOD样受体热蛋白结构域相关蛋白3(NLRP3)基因敲减对高脂高糖诱导的非酒精性脂肪性肝炎(NASH)小鼠模型的影响。方法将44只小鼠随机分为正常饮食组(CON)20只,高脂高糖造模组(HFHC)24只。造模14周末,随机选取4只HFHC组小鼠进行腺... 详细信息

目的探讨NOD样受体热蛋白结构域相关蛋白3(NLRP3)基因敲减对高脂高糖诱导的非酒精性脂肪性肝炎(NASH)小鼠模型的影响。方法将44只小鼠随机分为正常饮食组(CON)20只,高脂高糖造模组(HFHC)24只。造模14周末,随机选取4只HFHC组小鼠进行腺相关病毒9(AAV9)尾静脉注射预实验,4周后验证NLRP3敲减模型是否成功。18周末确认敲减成功后,对剩余40只小鼠进行AAV9一次性尾静脉注射,分为CON+NLRP3敲减阴性对照组(CON+NLRP3-NC)、CON+NLRP3敲减组(CON+NLRP3-KD)、HFHC+NLRP3-NC及HHFHC+NLRP3-KD组,每组10只,继续造模6周。24周末取材观察炎症小体活化效应,检测小鼠体质量、肝质量、肝指数及糖代谢(空腹血糖、空腹胰岛素及HOMA-IR指数)指标;检测小鼠肝脂质含量(肝组织TG及油红O染色)、肝脏炎症(血清ALT活性、HE染色及炎症相关基因)及肝纤维化(天狼星红染色及纤维化相关基因)指标。计量资料多组间比较使用单因素方差分析,进一步两两比较采用LSD-t检验。结果与CON+NLRP3-NC组相比,Western Blot结果提示,HFHC+NLRP3-NC组的NLRP3、pro-Caspase1、Caspase1、ASC及IL-1β蛋白水平均升高,HFHC+NLRP3-KD组均降低(P值均0.05)。结论NLRP3基因敲减可显著改善高脂高糖饮食诱导的NASH小鼠模型肝脂肪沉积及炎症。

关键词：非酒精性脂肪性肝病 NLR家族,热蛋白结构域包含蛋白3 膳食,高脂炎症小鼠,近交C57BL

维普期刊数据库博看期刊

Mitigating Antibiotic Resistance Emissions in the Pharmaceutical Industry: Global Governance and Available Techniques

在线全文

学校读者我要写书评

暂无评论

China CDC weekly 2023年第46期5卷 1038-1044页

作者： ziming Han Haodi feng Chen Wang Xuegong Wang Min Yang Yu Zhang State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijingChina National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery BeijingChina Sino-Danish College University of Chinese Academy of SciencesBeijingChina China Pharmaceutical Enterprises Association BeijingChina University of Chinese Academy of Sciences BeijingChina

We highlight the paramount importance of pretreatment processes preceding the biological treatment of antibiotic manufacturing wastewater as a strategy to curb the escalation of antibiotic resistance.In an effort to curtail the advancement of antimicrobial resistance(AMR)in antibiotic production in China,a comprehensive array of technical and regulatory initiatives have been implemented.These measures offer valuable insights that could inform and enhance global AMR containment strategies.Looking ahead,there is a pressing need to form an interdisciplinary team tasked with refining antibiotic emission standards and tailoring environmental engineering practices for the pharmaceutical sector.

关键词： wastewater Antibiotic measures

BaTiO_(3)基超薄层BME MLCC的可靠性机理

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

物理化学学报 2024年第1期40卷 32-41页

作者：朱超琼蔡子明冯培忠张伟晨惠可臻曹秀华付振晓王晓慧中国矿业大学材料与物理学院江苏徐州221116 清华大学材料学院新型陶瓷与精细工艺国家重点实验室北京100084 风华高新科技股份有限公司新型电子元器件关键材料与工艺国家重点实验室广东肇庆526000

多层陶瓷电容器(Multilayer ceramic capacitors,MLCC)作为市场占有率最高的无源电子元器件,是基础电子元件产业中需要突破关键技术的重点产品之一,在汽车电子、5G通讯、电网调频、航空航天等领域有广泛的应用。在小型化、薄层化发展趋... 详细信息

多层陶瓷电容器(Multilayer ceramic capacitors,MLCC)作为市场占有率最高的无源电子元器件,是基础电子元件产业中需要突破关键技术的重点产品之一,在汽车电子、5G通讯、电网调频、航空航天等领域有广泛的应用。在小型化、薄层化发展趋势下,MLCC的介质层厚度不断降低,单层介质在相同电压下的电场显著增大,尤其是中高压超薄层MLCC。因此,MLCC的可靠性愈发成为一项关键的产品质量指标。本文结合加速老化测试、高温阻抗谱、漏电流测试,系统研究超薄层MLCC的劣化机理,揭示抑制氧空位的迁移与富集是保证超薄层MLCC可靠性的重中之重。为此,应减小介质层内部的氧空位浓度,增大其迁移所需的激活能,提高界面肖特基势垒,从而提升超薄层MLCC的可靠性。本文的研究成果为超薄层MLCC介质材料的设计提供了有力指导。

关键词：钛酸钡多层陶瓷电容器可靠性加速老化氧空位

Effectiveness of Fuling(Poria) and its extracts against spleen deficiency in rats via tonifying spleen

在线全文

学校读者我要写书评

暂无评论

Journal of Traditional Chinese Medicine 2023年第3期43卷 501-506页

作者： feng Min JIA ziming WAN Ming FAN Bolin TANG Xiaoqiao CHENG Wenhua SUN Fanzhong Hubei Provincial Key Laboratory of Applied Toxicology Hubei Provincial Center for Disease Control and PreventionWuhan 430075China Department of pharmaceutical affairs Hubei Provincial Hospital of TCMWuhan 430074China

OBJECTIVE:To observe and explore the effect of Fuling(Poria) in alleviating the spleen deficiency symptom pattern(SDSP).METHODS:We established an animal model of SDS in Sprague-Dawley(SD) rats by treating them with deficiency-inducing factors,including irregular feeding and tail clamping.Mice were administered Fuling(Poria) and its extracts(raw/cooked powder,aqueous/alcohol extract) by gavage once a day for 21 d.The body weight,rectal temperature,and spleen and thymus organ coefficients were calculated.The levels of motilin(MTL),gastrin(GAS),aquaporin 2(AQP2),interleukin 2(IL-2),IL-4,and 5-hydroxytryptamine(5-HT) in the serum and the level of AQP2 in the kidneys were evaluated by enzyme-linked immunosorbent assay.RESULTS:Fuling(Poria) and its extracts did not change the body weight,rectal temperature,and organ coefficients of the spleen and thymus.However,it reduced the levels of MTL and GAS and increased the levels of IL-2 and AQP2.In addition,the levels of IL-4 and 5-HT showed no significant alteration.CONCLUSIONS:These results suggested the crucial function of Fuling(Poria) in SDSP,especially promoting digestive function and water metabolism.

关键词： Fuling(Poria) extracts spleen deficiency symptom pattern motilin gastrins interleukins aquaporin 2 serotonin

旋转锻造加工高比重W-Ni-Fe合金的退火工艺研究

在线全文

学校读者我要写书评

暂无评论

热加工工艺 2024年第6期53卷 62-66,71页

作者：李天瑞冯宏伟黄滢秋刘晓彬黄伟周朝红陈子明中国兵器科学研究院宁波分院浙江宁波315048 宁波甬业企业管理咨询有限公司浙江宁波315100

使用粉末冶金工艺制备Ni/Fe为7∶3的W-Ni-Fe高比重钨合金,在使用旋转锻造方法进行形变强化后进行热处理。结果表明,锻造态W-Ni-Fe合金经过一定温度的锻后退火,强度有所提高,而塑性下降。样品的强度在一定的退火温度和保温时间范围内能... 详细信息

使用粉末冶金工艺制备Ni/Fe为7∶3的W-Ni-Fe高比重钨合金,在使用旋转锻造方法进行形变强化后进行热处理。结果表明,锻造态W-Ni-Fe合金经过一定温度的锻后退火,强度有所提高,而塑性下降。样品的强度在一定的退火温度和保温时间范围内能得到提升,更高温度和更高时间的保温会使样品强度下降。不同钨含量的W-Ni-Fe合金,强度提升最大的退火温度和时间条件不同。SEM图像显示,退火前样品钨颗粒因旋转锻造变形呈现长条状织构,退火后钨颗粒的形状无明显变化。这些现象说明,锻造变形的合金样品经过锻后退火发生应变时效现象,使强度得到提升。研究明确了不同钨含量的W-Ni-Fe合金最佳的锻后退火工艺参数。

关键词：高比重钨合金 W-Ni-Fe合金旋转锻造退火

Check dam extraction from remote sensing images using deep learning and geospatial analysis:A case study in the Yanhe River Basin of the Loess Plateau,China

在线全文

学校读者我要写书评

暂无评论

Journal of Arid Land 2023年第1期15卷 34-51页

作者： SUN Liquan GUO Huili CHEN Ziyu YIN ziming feng Hao WU Shufang Kadambot H M SIDDIQUE Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas Ministry of EducationNorthwest A&F UniversityYangling 712100China Institute of Water-Saving Agriculture in Arid Areas of China Northwest A&F UniversityYangling 712100China College of Water Resources and Architectural Engineering Northwest A&F UniversityYangling 712100China College of Natural Resources and Environment Northwest A&F UniversityYangling 712100China The UWA Institute of Agriculture and School of Agriculture&Environment The University of Western AustraliaPerth 6001Australia

Check dams are widely used on the Loess Plateau in China to control soil and water losses,develop agricultural land,and improve watershed ecology.Detailed information on the number and spatial distribution of check dams is critical for quantitatively evaluating hydrological and ecological effects and planning the construction of new dams.Thus,this study developed a check dam detection framework for broad areas from high-resolution remote sensing images using an ensemble approach of deep learning and geospatial analysis.First,we made a sample dataset of check dams using GaoFen-2(GF-2)and Google Earth images.Next,we evaluated five popular deep-learning-based object detectors,including Faster R-CNN,You Only Look Once(version 3)(YOLOv3),Cascade R-CNN,YOLOX,and VarifocalNet(VFNet),to identify the best one for check dam detection.Finally,we analyzed the location characteristics of the check dams and used geographical constraints to optimize the detection results.Precision,recall,average precision at intersection over union(IoU)threshold of 0.50(AP_(50)),IoU threshold of 0.75(AP_(75)),and average value for 10 IoU thresholds ranging from 0.50-0.95 with a 0.05 step(AP_(50-95)),and inference time were used to evaluate model performance.All the five deep learning networks could identify check dams quickly and accurately,with AP_(50-95),AP_(50),and AP_(75)values higher than 60.0%,90.0%,and 70.0%,respectively,except for YOLOv3.The VFNet had the best performance,followed by YOLOX.The proposed framework was tested in the Yanhe River Basin and yielded promising results,with a recall rate of 87.0%for 521 check dams.Furthermore,the geographic analysis deleted about 50%of the false detection boxes,increasing the identification accuracy of check dams from 78.6%to 87.6%.Simultaneously,this framework recognized 568 recently constructed check dams and small check dams not recorded in the known check dam survey datasets.The extraction results will support efficient watershed management and guide future studies on soil erosion in the Loe

关键词： check dam deep learning geospatial analysis remote sensing Faster R-CNN Loess Plateau

产教融合“四方合作”在MIDD/MIPD背景下临床药学本科生创新培养模式的探索与实践

在线全文

学校读者我要写书评

暂无评论

卫生职业教育 2024年第12期42卷 4-8页

作者：王栋栋陈肖何素梅冯英成张村赵子明徐州医科大学药学院江苏徐州221004 徐州医科大学护理学院江苏徐州221004 南京大学医学院附属苏州医院江苏苏州215000 南京传奇生物科技有限公司江苏南京210000 徐州医科大学附属徐州东方医院江苏徐州221000 徐州医科大学教务处江苏徐州221004

模型引导的药物研发与精准给药(model-informed drug development and precision dosing,MIDD/MIPD)的核心是通过人工智能进行建模和仿真,旨在促进药物研发和个体化精准给药,通过产教融合学生—高校—药企—医院的“四方合作”形式创新... 详细信息

模型引导的药物研发与精准给药(model-informed drug development and precision dosing,MIDD/MIPD)的核心是通过人工智能进行建模和仿真,旨在促进药物研发和个体化精准给药,通过产教融合学生—高校—药企—医院的“四方合作”形式创新性地将MIDD/MIPD融入临床药学本科生的培养环节,短期内培养出适应我国社会需求的MIDD/MIPD背景临床药学高层次人才,提升我国从事新药研发与临床精准给药的临床药学人才队伍的综合实力。

关键词：产教融合 “四方合作” MIDD/MIPD 临床药学培养模式

寒冷地区堆石混凝土坝温度应力仿真分析——以东庄二道坝为例

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

水利与建筑工程学报 2024年第1期22卷 31-38页

作者：陆子铭赵厚然杨俊峰金峰徐艳杰清华大学水利水电工程系北京100084 陕西省东庄水利枢纽工程建设有限责任公司陕西西安713200

针对寒区堆石混凝土坝内外温差引起坝体裂缝问题,以东庄二道坝为依托,基于工程图纸进行网格划分和三维建模,进行三维有限元模型计算,对堆石混凝土重力坝温度应力场进行仿真分析,并结合工程实测数据进行参数反演标定。结果表明:施工期坝... 详细信息

针对寒区堆石混凝土坝内外温差引起坝体裂缝问题,以东庄二道坝为依托,基于工程图纸进行网格划分和三维建模,进行三维有限元模型计算,对堆石混凝土重力坝温度应力场进行仿真分析,并结合工程实测数据进行参数反演标定。结果表明:施工期坝体拉应力在冬季呈现较高水平,拉应力主要出现在坝体上下游面一定厚度的表层区,尤以中下部高程的抗冲耐磨混凝土拉应力最大;在冬季内部拉应力明显高于坝的两侧,两个坝段宽度方向均出现超过允许应力的情况。建议将短缝加深四层有限元单元,坝体稳定性得到较大提高,工程量相对较小,既安全又经济,为最优方案。

关键词：堆石混凝土仿真分析温度应力有限元法分缝方案

Three-Year Consecutive Field Application of Erythromycin Fermentation Residue Following Hydrothermal Treatment:Cumulative Effect on Soil Antibiotic Resistance Genes

在线全文

学校读者我要写书评

暂无评论

Engineering 2022年第8期8卷 78-88页

作者： ziming Han Haodi feng Xiao Luan Yunpeng Shen Liren Ren Liujie Deng D.G.Joakim Larsson Michael Gillings Yu Zhang Min Yang State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China State Environmental Protection Engineering Center for Harmless Treatment and Resource Utilization of Antibiotic Residues Khorgos 835007China Institute for Biomedicine&the Centre for Antibiotic Resistance Research University of GothenburgGoteborg SE-41346Sweden Australian Research Council(ARC)Centre of Excellence in Synthetic Biology Department of Biological SciencesFaculty of Science and EngineeringMacquarie UniversitySydneyNSW 2109Australia

Fermentation-based antibiotic production results in abundant nutrient-rich fermentation residue with high potential for recycling,but the high antibiotic residual concentration restricts its usefulness(e.g.,in land application as organic fertilizer).In this study,an industrial-scale hydrothermal facility for the treatment of erythromycin fermentation residue(EFR)was investigated,and the potential risk of the long-term soil application of treated EFR promoting environmental antibiotic resistance development was evaluated.The treatment effectively removed bacteria and their DNA,and an erythromycin removal ratio of up to approximately 98%was achieved.The treated EFR was utilized as organic fertilizer for consecutive field applications from 2018 to 2020,with dosages ranging from 3750 to 15000 kg·hm^(-2),resulting in subinhibitory levels of erythromycin(ranging from 0.83-76.00μg·kg^(-1))in soils.Metagenomic shotgun sequencing was then used to characterize the antibiotic resistance genes(ARGs),mobile genetic elements(MGEs),and bacterial community composition of the soils.The soil ARG abundance and diversity did not respond to the treated EFR application in the first year,but gradually changed in the second and third year of application.The highest fold change in relative abundance of macrolide-lincosamide-streptogramin(MLS)and total ARGs were 12.59 and 2.75 times,compared with the control(CK;without application),respectively.The soil MGEs and taxonomic composition showed similar temporal trends to those of the ARGs,and appeared to assist in driving increasing ARG proliferation,as revealed by correlation analysis and structural equation models(SEMs).The relative abundance of particular erm resistance genes(RNA methyltransferase genes)increased significantly in the third year of treated EFR application.The close association of erm with MGEs suggested that horizontal gene transfer played a critical role in the observed erm gene enrichment.Metagenomic binning results demonstrated that the proliferation of mac genecarrying

关键词： Pharmaceutical manufacturing Resistome Antibiotic Risk assessment Remediation Land application