Three-Year Consecutive Field Application of Erythromycin Fermentation Residue Following Hydrothermal Treatment:Cumulative Effect on Soil Antibiotic Resistance Genes

作     者：Ziming Han Haodi Feng Xiao Luan Yunpeng Shen Liren Ren Liujie Deng D.G.Joakim Larsson Michael Gillings Yu Zhang Min Yang Ziming Han;Haodi Feng;Xiao Luan;Yunpeng Shen;Liren Ren;Liujie Deng;D.G.Joakim Larsson;Michael Gillings;Yu Zhang;Min Yang

作者机构：State Key Laboratory of Environmental Aquatic ChemistryResearch Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China University of Chinese Academy of SciencesBeijing 100049China State Environmental Protection Engineering Center for Harmless Treatment and Resource Utilization of Antibiotic ResiduesKhorgos 835007China Institute for Biomedicine&the Centre for Antibiotic Resistance ResearchUniversity of GothenburgGoteborg SE-41346Sweden Australian Research Council(ARC)Centre of Excellence in Synthetic BiologyDepartment of Biological SciencesFaculty of Science and EngineeringMacquarie UniversitySydneyNSW 2109Australia 

出 版 物：《Engineering》 (工程（英文）)

年 卷 期：2022年第8卷第8期

页      面：78-88页

核心收录：

学科分类：12[管理学] 1204[管理学-公共管理] 082803[工学-农业生物环境与能源工程] 07[理学] 08[工学] 0828[工学-农业工程] 09[农学] 0903[农学-农业资源与环境] 120405[管理学-土地资源管理] 0713[理学-生态学] 

基　　金：obtained from National Natural Science Foundation of China(32141002 and 22076203) 

主　　题：Pharmaceutical manufacturing Resistome Antibiotic Risk assessment Remediation Land application 

摘      要：Fermentation-based antibiotic production results in abundant nutrient-rich fermentation residue with high potential for recycling,but the high antibiotic residual concentration restricts its usefulness(e.g.,in land application as organic fertilizer).In this study,an industrial-scale hydrothermal facility for the treatment of erythromycin fermentation residue(EFR)was investigated,and the potential risk of the long-term soil application of treated EFR promoting environmental antibiotic resistance development was *** treatment effectively removed bacteria and their DNA,and an erythromycin removal ratio of up to approximately 98%was *** treated EFR was utilized as organic fertilizer for consecutive field applications from 2018 to 2020,with dosages ranging from 3750 to 15000 kg·hm^(-2),resulting in subinhibitory levels of erythromycin(ranging from 0.83-76.00μg·kg^(-1))in *** shotgun sequencing was then used to characterize the antibiotic resistance genes(ARGs),mobile genetic elements(MGEs),and bacterial community composition of the *** soil ARG abundance and diversity did not respond to the treated EFR application in the first year,but gradually changed in the second and third year of *** highest fold change in relative abundance of macrolide-lincosamide-streptogramin(MLS)and total ARGs were 12.59 and 2.75 times,compared with the control(CK;without application),*** soil MGEs and taxonomic composition showed similar temporal trends to those of the ARGs,and appeared to assist in driving increasing ARG proliferation,as revealed by correlation analysis and structural equation models(SEMs).The relative abundance of particular erm resistance genes(RNA methyltransferase genes)increased significantly in the third year of treated EFR *** close association of erm with MGEs suggested that horizontal gene transfer played a critical role in the observed erm gene *** binning results demonstr