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Dual-crosslinked regenerative hydrogel for sutureless long-term repair of corneal defect

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Bioactive Materials 2023年第2期20卷 434-448页

作者： Xuanren Shen Saiqun Li Xuan Zhao Jiandong Han Jiaxin Chen zilong rao Kexin Zhang Daping Quan Jin Yuan Ying Bai Guangdong Engineering Technology Research Centre for Functional Biomaterials School of Materials Science and EngineeringSun Yat-sen UniversityGuangzhou510006China State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou510623China

Corneal transplantation is the most effective clinical treatment for corneal defects,but it requires precise size of donor corneas,surgical sutures,and overcoming other technical *** patients may suffer graft rejection and complications caused by *** glues that can long-term integrate with the corneal tissue and effectively repair the focal corneal damage are highly ***,a hybrid hydrogel consisting of porcine decellularized corneal stroma matrix(pDCSM)and methacrylated hyaluronic acid(HAMA)was developed through a non-competitive dual-crosslinking *** can be directly filled into corneal defects with various *** importantly,through formation of interpenetrating network and stable amide bonds between the hydrogel and adjacent tissue,the hydrogel manifested excellent adhesion properties to achieve suture-free ***,the hybrid hydrogel not only preserved bioactive components from pDCSM,but also exhibited cornea-matching transparency,low swelling ratio,slow degradation,and enhanced mechanical properties,which was capable of withstanding superhigh intraocular *** combinatorial hydrogel greatly improved the poor cell adhesion performance of HAMA,supported the viability,proliferation of corneal cells,and preservation of keratocyte *** a rabbit corneal stromal defect model,the experimental eyes treated with the hybrid hydrogel remained transparent and adhered intimately to the stroma bed with long-term retention,accelerated corneal re-epithelialization and wound *** the advantages of high bioactivity,low-cost,and good practicality,the dual-crosslinked hybrid hydrogel served effectively for long-term suture-free treatment and tissue regeneration after corneal defect.

关键词： Corneal defect Decellularized extracellular matrix Hydrogel Sutureless repair Cornea regeneration

Decellularized Extracellular Matrix Containing Electrospun Fibers for Nerve Regeneration:A Comparison Between Core–Shell Structured and Preblended Composites

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Advanced Fiber Materials 2022年第3期4卷 503-519页

作者： Rongli Deng Ziling Luo zilong rao Zudong Lin Shihao Chen Jing Zhou Qingtang Zhu Xiaolin Liu Ying Bai Daping Quan PCFM Lab GD HPPC LabSchool of ChemistrySun YatSen UniversityGuangzhou 510006China Guangdong Engineering Technology Research Centre for Functional Biomaterials School of Materials Science and EngineeringSun Yat-Sen UniversityGuangzhou 510006China Guangdong Peripheral Nerve Tissue Engineering and Technology Research Center Department of Orthopedic and MicrosurgeryThe First Afliated Hospital of Sun Yat-Sen UniversityGuangzhou 510080China Guangdong Provincial Soft Tissue Biofabrication Engineering Laboratory GuangdongGuangzhou 510080China

Advanced biomaterial-based strategies for treatment of peripheral nerve injury require precise control over both topological and biological cues for facilitating rapid and directed nerve *** a highly bioactive and tissue-specifc natural material,decellularized extracellular matrix(dECM)derived from peripheral nerves(decellularized nerve matrix,DNM)has drawn increasing attention in the feld of regenerative medicine,due to its outstanding capabilities in facilitating neurite outgrowth and *** induce and maintain sufcient topological guidance,electrospinning was conducted for fabrication of axially aligned nanofbers consisting of DNM and poly(ε-caprolactone)(PCL).Core–shell structured fbers were prepared by coaxial electrospinning using DNM as the shell and PCL as the *** to the aligned electrospun fbers using preblended DNM/PCL,the core–shell structured fbers exhibited lower tensile strength,faster degradation,but considerable toughness for nerve guidance conduit preparation and relatively intact fbrous structure after long-term *** importantly,the full DNM surface coverage of the aligned core–shell fbers efectively promoted axonal extension and Schwann cells *** DNM contents further triggered neurite bundling and myelin formation toward nerve fber maturation and ***,we not only pursue a multi-functional scafold design for nerve regeneration,a detailed comparison between core–shell structured and preblended electrospinning of DNM/PCL composites was also provided as an applicable paradigm for advanced tissue-engineered strategies using dECM-based biomaterials.

关键词： Decellularized extracellular matrix Coaxial electrospinning Core–shell structure Preblended composites Nerve regeneration

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魏稼针灸治学历程与学术成就

江西中医药大学学报 2024年第1期36卷 8-10,13页

作者：罗志强刘柏霖徐涵斌何勇饶子龙宋南昌泰和县中医院江西泰和343700 上海中医药大学上海201203 江西省中西医结合医院南昌330003

对魏稼针灸文献研究及学术成就进行总结。魏稼对于针灸学发展具有前瞻性思维,提出热证可灸、敏穴、无创痛穴疗法等新观点、新理论;积极筹办、领导中国针灸学会针灸文献研究专业委员会,组织参与学术交流;创立“各家针灸学说”新学科;在... 详细信息

对魏稼针灸文献研究及学术成就进行总结。魏稼对于针灸学发展具有前瞻性思维,提出热证可灸、敏穴、无创痛穴疗法等新观点、新理论;积极筹办、领导中国针灸学会针灸文献研究专业委员会,组织参与学术交流;创立“各家针灸学说”新学科;在继承的基础上创新,融入现代医学,丰富了针灸理论,拓宽了针灸研究领域。

关键词：魏稼针灸文献各家针灸学说针灸学会学术成就

Decellularized Disc Hydrogels for hBMSCs tissue-specific differentiation and tissue regeneration

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Bioactive Materials 2021年第10期6卷 3541-3556页

作者： Yizhong Peng Xiangcheng Qing Hui Lin Donghua Huang Jinye Li Shuo Tian Sheng Liu Xiao Lv Kaige Ma Rui Li zilong rao Ying Bai Songfeng Chen Ming Lei Daping Quan Zengwu Shao Department of Orthopaedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Musculoskeletal Tumor Center Department of OrthopedicsThe Second Affiliated Hospital of Zhejiang University School of MedicineHangzhouChina School of Chemistry Sun Yat-sen UniversityGuangzhou510127China School of Materials Science and Engineering Sun Yat-sen UniversityGuangzhou510127China Department of Orthopaedic Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhou City450052China

Tissue specificity,a key factor in the decellularized tissue matrix(DTM),has shown bioactive functionalities in tuning cell fate-e.g.,the differentiation of mesenchymal stem ***,cell fate is also determined by the living microenvironment,including material composition and spatial ***,two neighboring tissues within intervertebral discs,the nucleus pulposus(NP)and annulus fibrosus(AF),were carefully processed into DTM hydrogels(abbreviated DNP-G and DAF-G,respectively)to determine the tissue-specific effects on stem cell fate,such as specific components and different culturing methods,as well as in vivo *** differences in their protein compositions were identified by proteomic ***,the fate of human bone marrow mesenchymal stem cells(hBMSCs)also responds to both culturing methods and ***,hBMSCs cultured with DNP-G(3D)differentiated into NP-like cells,while hBMSCs cultured with DAF-G(2D)underwent AF-like differentiation,indicating a close correlation with the native microenvironments of NP and AF cells,***,we found that the integrin-mediated RhoA/LATS/YAP1 signaling pathway was activated in DAF-G(2D)-induced AF-specific ***,the activation of YAP1 determined the tendency of NP-or AF-specific differentiation and played opposite regulatory ***,DNP-G and DAF-G specifically promoted tissue regeneration in NP degeneration and AF defect rat models,*** conclusion,DNP-G and DAF-G can specifically determine the fate of stem cells through the integrin-mediated RhoA/LATS/YAP1 signaling pathway,and this tissue specificity is both compositional and spatial,supporting the utilization of tissue-specific DTM in advanced treatments of intervertebral disc degeneration.

关键词： Tissue specificity Decellularized tissue matrix Intervertebral disc Differentiation YAP1

Harnessing decellularised extracellular matrix microgels into modular bioinks for extrusion-based bioprinting with good printability and high post-printing cell viability

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Biomaterials Translational 2023年第2期4卷 115-127页

作者： Hanyu Chu Kexin Zhang zilong rao Panpan Song Zudong Lin Jing Zhou Liqun Yang Daping Quan Ying Bai Key Laboratory for Polymeric Composite&Functional Materials of Ministry of Education School of ChemistrySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Guangdong Engineering Technology Research Centre for Functional Biomaterials Key Laboratory for Polymeric Composite&Functional Materials of Ministry of EducationSchool of Materials Science and EngineeringSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina

The printability of bioink and post-printing cell viability is crucial for extrusion-based bioprinting.A proper bioink not only provides mechanical support for structural fidelity,but also serves as suitable three-dimensional(3D)microenvironment for cell encapsulation and *** this study,a hydrogel-based composite bioink was developed consisting of gelatin methacryloyl(GelMA)as the continuous phase and decellularised extracellular matrix microgels(DMs)as the discrete phase.A flow-focusing microfluidic system was employed for the fabrication of cell-laden DMs in a high-throughput *** gentle mixing of the DMs and GelMA,both rheological characterisations and 3D printing tests showed that the resulting DM-GelMA hydrogel preserved the shear-thinning nature,mechanical properties,and good printability from *** integration of DMs not only provided an extracellular matrix-like microenvironment for cell encapsulation,but also considerable shear-resistance for high post-printing cell *** DM sizes and inner diameters of the 3D printer needles were correlated and optimised for nozzle-based ***,a proof-of-concept bioink composedg of RSC96 Schwann cells encapsulated DMs and human umbilical vein endothelial cell-laden GelMA was successfully bioprinted into 3D constructs,resulting in a modular co-culture system with distinct cells/materials ***,the modular DM-GelMA bioink provides a springboard for future precision biofabrication and will serve in numerous biomedical applications such as tissue engineering and drug screening.

关键词： 3D bioprinting bioinks cell viability decellularised extracellular matrix microgels

A comparative study of human and porcine-derived decellularised nerve matrices

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Biomaterials Translational 2023年第3期4卷 180-195页

作者： Rui Li Shuai Qiu Weihong Yang zilong rao Jiaxin Chen Yuexiong Yang Qingtang Zhu Xiaolin Liu Ying Bai Daping Quan Guangdong Engineering Technology Research Centre for Functional Biomaterials School of Materials Science and EngineeringSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Key Laboratory for Polymeric Composite&Functional Materials of Ministry of Education School of ChemistrySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Guangdong Engineering Technology Research Centre for Peripheral Nerve Tissue Department of Orthopaedic and MicrosurgeryThe First Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Guangzhou Zhongda Medical Equipment Co. Ltd.GuangzhouGuangdong ProvinceChina

Decellularised extracellular matrix(dECM)biomaterials originating from allogeneic and xenogeneic tissues have been broadly studied in the field of regenerative medicine and have already been used in clinical *** dECMs are considered more compatible,but they have the drawback of extremely limited human tissue *** availability is also restricted by the health and age of the *** investigate the viability of xenogeneic tissues as a substitute for human tissues,we fabricated both porcine decellularised nerve matrix(pDNM)and human decellularised nerve matrix for a comprehensive *** showed that both dECM scaffolds retained the ECM microstructures of native human nerve *** analysis demonstrated that the protein compositions of both dECMs were also very similar to each *** functional ECM contents effectively promoted the proliferation,migration,and maturation of primary human Schwann cells in ***,pDNM contained a few antigens that induced severe host immune responses in humanised ***,after removing theα-galactosidase antigen,the immune responses were highly alleviated and the pre-treated pDNM maintained a human decellularised nerve matrix-like pro-regenerative ***,we believe that anα-galactosidase-free pDNM may serve as a viable substitute for human decellularised nerve matrix in future clinical applications.

关键词： allogeneic decellularised nerve matrix immune response xenogeneic α-Gal

Research and Discussion on Flipped Classroom Combined with Case-Based Learning in Pharmacoeconomics Teaching

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Journal of Contemporary Educational Research 2024年第4期8卷 120-125页

作者： Xingwen Zhou zilong Dang Xingdong Wang Chen Chen Zhi rao Ting Wei Yanping Wang Department of Pharmacy the First Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina

Objective:To explore the application effect of flipped classroom combined with case-based learning teaching methods in pharmacoeconomics ***:The students majoring in clinical pharmacy in 2019 were selected as the study subjects,and the cost-effectiveness analysis of different dosage forms of Yinzhihuang in the treatment of neonatal jaundice was selected as the teaching *** flipped classroom combined with case-based learning teaching method was used to carry out theoretical teaching to the *** the course,questionnaires were distributed through the Sojump platform to evaluate the teaching ***:The results of the questionnaire showed that 85.71%of the students believed that the flipped classroom combined with case-based learning teaching method was helpful in mobilizing the learning enthusiasm and initiative,and improving the comprehensive application ability of the knowledge of pharmacoeconomics.92.86%of the students think that it is conducive to the understanding and memorization of learning content,as well as the cultivation of teamwork,communication,***:Flipped classroom combined with case-based learning teaching method can improve students’knowledge mastery,thinking skills,and practical application skills,as well as optimize and improve teachers’teaching levels.

关键词： Flipped classroom Case-based learning teaching method Pharmacoeconomics Teaching methods

循经针刺治疗腰椎间盘突出症的效果及对腰椎功能和血清炎症因子水平的影响

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中国医学创新 2023年第35期20卷 54-58页

作者：何亮饶子龙陈茂风江西省中西医结合医院江西南昌330003

目的:观察循经针刺治疗腰椎间盘突出症的效果及对腰椎功能和血清炎症因子水平的影响。方法:研究对象为2020年1月—2021年12月江西省中西医结合医院收治的80例腰椎间盘突出症患者,将其随机分为对照组和观察组,每组40例。对照组采取常规... 详细信息

目的:观察循经针刺治疗腰椎间盘突出症的效果及对腰椎功能和血清炎症因子水平的影响。方法:研究对象为2020年1月—2021年12月江西省中西医结合医院收治的80例腰椎间盘突出症患者,将其随机分为对照组和观察组,每组40例。对照组采取常规西药治疗,观察组采用西药联合循经针刺治疗。比较两组症状积分、腰椎功能[日本骨科协会评估治疗(JOA)评分]、血清炎症因子[C反应蛋白(CRP)、白介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)]水平、下肢功能障碍情况[直腿抬高角度、Oswestry功能障碍指数(ODI)]及疗效。结果:治疗后观察组各项症状积分及总分均低于对照组(P<0.05);治疗后观察组JOA各维度评分及总分均高于对照组(P<0.05);治疗后两组CRP、IL-6及TNF-α水平均降低,且观察组均低于对照组(P<0.05);治疗后两组ODI评分均下降,且观察组较对照组更低(P<0.05),两组直腿抬高角度均大于治疗前,且观察组较对照组更大(P<0.05);观察组总有效率较对照组更高(P<0.05)。结论:循经针刺治疗腰椎间盘突出症的效果明显,能有效改善患者的腰椎功能及血清炎症因子水平。

关键词：循经针刺腰椎间盘突出症腰椎功能炎症因子

2020年福建省某市小型私营煤矿企业粉尘职业健康检查现状分析

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职业卫生与应急救援 2023年第5期41卷 581-584页

作者：李德新饶子龙王芳三明市疾病预防控制中心福建三明365000

目的了解2020年福建省某市小型私营煤矿企业接触粉尘人员职业健康检查结果,为制定煤矿企业工人的尘肺病防治措施提供科学依据。方法收集2020年该市小型私营煤矿企业粉尘职业健康检查的个案卡数据,分析X射线胸片疑似尘肺样改变及肺功能... 详细信息

目的了解2020年福建省某市小型私营煤矿企业接触粉尘人员职业健康检查结果,为制定煤矿企业工人的尘肺病防治措施提供科学依据。方法收集2020年该市小型私营煤矿企业粉尘职业健康检查的个案卡数据,分析X射线胸片疑似尘肺样改变及肺功能异常检出率等核心指标情况,并对结果进行统计学分析。结果2020年该市小型私营煤矿企业接触粉尘工人参与职业健康检查共1330人次,以男性(占84.0%)、35岁以上(占90.8%)、接尘工龄0.05)。结论该市小型私营煤矿企业作业人员流动性大,疑似尘肺样改变检出率高。煤工尘肺主要引起限制性通气功能障碍。须提高小型私营煤矿企业职业病防治意识,进一步重视流动从业人员的职业接触史和职业健康状况,及时采取预防措施。

关键词：小型私营煤矿粉尘职业健康检查尘肺样改变肺功能