A comparative study of human and porcine-derived decellularised nerve matrices
作者机构：Guangdong Engineering Technology Research Centre for Functional BiomaterialsSchool of Materials Science and EngineeringSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Key Laboratory for Polymeric Composite&Functional Materials of Ministry of EducationSchool of ChemistrySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Guangdong Engineering Technology Research Centre for Peripheral Nerve TissueDepartment of Orthopaedic and MicrosurgeryThe First Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Guangzhou Zhongda Medical Equipment Co.Ltd.GuangzhouGuangdong ProvinceChina
出 版 物：《Biomaterials Translational》 (生物材料转化电子杂志（英文）)
年 卷 期：2023年第4卷第3期
页 面：180-195页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：the National Natural Science Foundation of China(Nos.51903255 and 52073314) The Key Areas Research and Development Program of Guangdong Province(No.2020B1111150003) Science and Technology Program of Guangzhou City(No.201904010364)
主 题：allogeneic decellularised nerve matrix immune response xenogeneic α-Gal
摘 要：Decellularised extracellular matrix(dECM)biomaterials originating from allogeneic and xenogeneic tissues have been broadly studied in the field of regenerative medicine and have already been used in clinical *** dECMs are considered more compatible,but they have the drawback of extremely limited human tissue *** availability is also restricted by the health and age of the *** investigate the viability of xenogeneic tissues as a substitute for human tissues,we fabricated both porcine decellularised nerve matrix(pDNM)and human decellularised nerve matrix for a comprehensive *** showed that both dECM scaffolds retained the ECM microstructures of native human nerve *** analysis demonstrated that the protein compositions of both dECMs were also very similar to each *** functional ECM contents effectively promoted the proliferation,migration,and maturation of primary human Schwann cells in ***,pDNM contained a few antigens that induced severe host immune responses in humanised ***,after removing theα-galactosidase antigen,the immune responses were highly alleviated and the pre-treated pDNM maintained a human decellularised nerve matrix-like pro-regenerative ***,we believe that anα-galactosidase-free pDNM may serve as a viable substitute for human decellularised nerve matrix in future clinical applications.
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