检索结果-南通市图书馆

Slc20a1b is essential for hematopoietic stem/progenitor cell expansion in zebrafish

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2021年第12期64卷 2186-2201页

作者： Jiakui Chen Gaofei Li Junwei Lian Ning Ma Zhibin Huang Jianchao Li zilong wen wenqing Zhang Yiyue Zhang Department of Developmental Biology School of Basic Medical SciencesSouthern Medical UniversityGuangzhou 510515China Division of Cell Developmental and Integrative BiologySchool of MedicineSouth China University of TechnologyGuangzhou 510006China Division of Life Science State Key Laboratory of Molecular NeuroscienceHong Kong University of Science and TechnologyHong KongChina

Hematopoietic stem and progenitor cells(HSPCs)are able to self-renew and can give rise to all blood lineages throughout their lifetime,yet the mechanisms regulating HSPC development have yet to be *** this study,we characterized a hematopoiesis defective zebrafish mutant line named smu07,which was obtained from our previous forward genetic screening,and found the HSPC expansion deficiency in the *** cloning identified that slc20a1b,which encodes a sodium phosphate cotransporter,contributed to the smu07 blood *** analysis demonstrated that mutation of slc20a1b affects HSPC expansion through cell cycle arrest at G2/M phases in a cell-autonomous *** study shows that slc20a1b is a vital regulator for HSPC proliferation in zebrafish early hematopoiesis and provides valuable insights into HSPC development.

关键词： Slc20a1b HSPC zebrafish

The Origin and Colonization of Microglia

在线全文

学校读者我要写书评

暂无评论

The Origin and Colonization of Microglia

Nlrc3-like is required for microglia maintenance in zebrafish

中国神经科学学会第十二届全国学术会议

作者： wen zilong Hong Kong University of Science and Technology

Microglia are central nervous system（CNS）-resident macrophages and play pivotal roles in neural development and neural function. Previous studies in mice have suggested that microglia are derived from the yolk sac（YS） where embryonic haematopoiesis is known to occur. Because these studies lack spatial resolution, it remains debatable whether the mouse YS is the sole bona fide source for microglia, and moreover, how microglial precursors born in peripheral tissues colonize the brain is completely undefined. Here, using an infrared light-induced temporal-spatial resolved fate mapping analysis, we challenge this single source view by showing that microglia in zebrafish arise from multiple sources. We show that the embryonic/larval microglia originate from the rostral blood island（RBI）, the zebrafish counterpart of mouse YS for myelopoiesis, whereas the adult microglia arise from the ventral wall of dorsal aorta（VDA）, a tissue equivalent to the mouse aorta, gonads, and mesonephros（AGM） where hematopoietic stem cells emerge. Using in vivo imaging and genetic manipulation, we further show that the RBI-derived microglial precursors enter the developing zebrafish brain via the lateral periphery between the eyes and brain and the ventral periphery of the brain in a circulation-independent manner. Intriguingly, the colonization of the brain by microglial precursors is dynamic and driven by apoptotic neurons through releasing lysophosphatidylcholine.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2019年第6期46卷 291-299页

作者： Tienan Wang Bo Yan Liang Lou Xi Lin Tao Yu Shuting Wu Qing Lu Wei Liu Zhibin Huang Mingjie Zhang wenqing Zhang zilong wen Division of Life Science and State Key Laboratory of Molecular Neuroscience Center of Systems Biology and Human Health The Hong Kong University of Science and Technology Clearwater Bay Kowloon Hong Kong China Shanghai Public Health Clinical Center Fudan University Shanghai 201508 China Bio-X Institutes Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders Ministry of Education Shanghai Jiao Tong University Shanghai 200240 China Department of Developmental Biology School of Basic Medical Sciences South China University of Technology Guangzhou 510630 China Division of Life Science Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong China

Microglia are tissue-resident macrophages residing in the central nervous system (CNS) and play critical roles in removing cellular debris and infectious agents as well as regulating neurogenesis and neuronal activities. Yet, the molecular basis underlying the establishment of microglia pool and the maintenance of their homeostasis in the CNS remain largely undefined. Here we report the identification and characterization of a mutant zebrafish, which harbors a point mutation in the nucleotide-binding oligomerization domain (NOD) like receptor gene nlrc3-like, resulting in the loss of microglia in a temperature sensitive manner. Temperature shift assay reveals that the late onset of nlrc3-like deficiency leads to excessive microglia cell death. Further analysis shows that the excessive microglia death in nlrc3-like deficient mutants is attributed, at least in part, to aberrant activation of canonical inflammasome pathway. Our study indicates that proper regulation of inflammasome cascade is critical for the maintenance of microglia homeostasis.

关键词： Zebrafish Microglia Inflammasome NOD-like receptors

Tissue-resident macrophages: from zebrafish to mouse

在线全文

学校读者我要写书评

暂无评论

Blood Science 2019年第1期1卷 57-60页

作者： Xi Lin zilong wen Jin Xu Division of Life Science State Key Laboratory of Molecular Neuroscience and Center of Systems Biology and Human HealthHong Kong University of Science and TechnologyClear Water BayKowloonHong KongP.R.China Division of Cell Developmental and Integrative BiologySchool of MedicineSouth China University of TechnologyGuangzhouChina

Tissue-resident macrophages(TRMs),generally found in tissues under normal physiological conditions,play crucial roles not only in immunity but also in tissue development and *** of their diverse functions,dysregulation of their development and function has been implicated in many human *** the past decade,a great deal of extensive studies have been conducted in various model organisms with cutting-edge technologies to explore the origin and function of *** this review,we summarize the recent findings on TRMs in mouse and zebrafish and compare the similarity/differences between these two species.

关键词： Erythromyeloid precursor Hematopoiesis Hematopoietic stem cell Metaphocyte Mouse Tissue-resident macrophage Zebrafish

Fli1 acts in parallel with Pu.1 to control macrophage and neutrophil fate in zebrafish

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2024年第3期51卷 359-362页

作者： Ao Zhang Jingao Lu Shachuan Feng Huapeng Yu Tao Yu Shizheng Zhao Kemin Chen Zhibin Huang Jin Xu Jianan Y.Qu zilong wen Division of Life Science Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina Division of Cell Developmental and Integrative BiologySchool of MedicineSouth China University of TechnologyGuangzhouGuangdong 510006China Department of Immunology and Microbiology School of Life ScienceSouthern University of Science and TechnologyShenzhenGuangdong 518055China Biomedical Research Institute Shenzhen Peking University-Hong Kong University of Science and Technology Medical CenterShenzhenGuangdong 518036China Department of Electronic and Computer Engineering Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina Greater Bay Biomedical Innocenter Shenzhen Bay LaboratoryShenzhenGuangdong 518067China

Macrophages and neutrophils are key components of myeloid cells and play critical roles in innate immune responses,organ formation,and tissue *** integrity of their functions heavily relies on the generation of a proper number of mature macrophages and neutrophils through embryonic and adult *** mammalian adult myelopoiesis,oligopotent common myeloid progenitors(CMPs)are known to be the earliest myeloid progenitors,which give rise to granulocyte-macrophage progenitors(GMPs),subsequently differentiate into unipotent neutrophil and macrophage precursors,and finally,mature macrophages and neutrophils(Orkin and Zon,2008).In contrast,the ontogeny of embryonic myelopoiesis and the mechanism underlying the formation of macrophages and neutrophils remainless understood.

关键词： fish macrophage homeostasis

Pu.1-lrf8 Pathway Regulate Monoccyte Specification during Zebrafish Embryogenesis

在线全文

学校读者我要写书评

暂无评论

Pu.1-lrf8 Pathway Regulate Monoccyte Specification during Ze...

黄药类捕收剂对嗜酸氧化亚铁硫杆菌的毒害机理

2010·第一届斑马鱼学术研讨会

作者： zilong wen Hong Kong University of Science and Technology

Myeloid cells are major cellular components of blood and essential regulators of host defense,embryo- and organo-genesis,and tissue *** mammals they are classifies into two major lineages:granulocytic and monocytic li...

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

化工学报 2012年第6期63卷 1883-1887页

作者：董颖博林海温子龙金属矿山高效开采与安全教育部重点实验室北京科技大学土木与环境工程学院北京100083

引言随着微生物技术的发展及在矿物加工工程领域内的应用,生物浸矿技术越来越受到重视,国内外在这一领域的研究近年来突飞猛进,从浸矿用微生物的选育、

关键词：嗜酸氧化亚铁硫杆菌黄药类捕收剂毒害机理吸附作用

在线全文

学校读者我要写书评

暂无评论

群体遗传学下动物驯化研究进展

遗传 2021年第3期43卷 226-239页

作者：文子龙赵毅强中国农业大学生物学院北京100193

动物驯化是将野生动物改变为能够长期稳定饲养的家养动物的过程。作为新石器时代农业革命的内容,驯化是人类社会文明进步的重要标志之一。由于和人类的密切关系,驯化不仅改变了动物的野生状态,也改变了人类的生活习性和文明进程。动物... 详细信息

动物驯化是将野生动物改变为能够长期稳定饲养的家养动物的过程。作为新石器时代农业革命的内容,驯化是人类社会文明进步的重要标志之一。由于和人类的密切关系,驯化不仅改变了动物的野生状态,也改变了人类的生活习性和文明进程。动物驯化研究的关键问题包含驯化祖先是谁、驯化所产生的改变及驯化时间地点等。随着高通量基因组技术和对应分析方法的发展,目前研究动物驯化一般基于群体水平,在群体遗传学的框架下研究动物驯化过程中的重要事件。本文总结了群体遗传学下动物驯化研究的相关内容,包括群体动态历史、选择信号、基因交流等,着重介绍了基因选择初始时间和基因交流时间两个新的拓展内容及分析方法,概述了家猪(Sus scrofa ***)、家鸡(Gallus gallus domesticus)、绵羊(Ovis aries)和山羊(Caprine hircus)等几种主要农业动物近期驯化研究的进展,以期为动物驯化研究提供了新的方向和视角。

关键词：驯化家养动物群体遗传学选择初始时间基因交流时间

Cebpa is essential for the embryonic myeloid progenitor and neutrophil maintenance in zebrafish

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2016年第10期43卷 593-600页

作者： Yimei Dai Lu Zhu Zhibin Huang Minyu Zhou Wan Jin Wei Liu Mengchang Xu Tao Yu Yiyue Zhang zilong wen Wangjun Liao wenqing Zhang Division of Life Science State Key Laboratory of Molecular Neuroscience and Center of Systems Biology and Human Health The Hong Kong University of Science and Technology Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes Department of Cell BiologySchool of Basic Medical Sciences Southern Medical University Department of Oncology Nanfang Hospital Southern Medical University

In vertebrates, myeloid cells arise from multiple waves of development： the first or embryonic wave of myelopoiesis initiates early from non-hematopoietic stem cell（HSC） precursors and gives rise to myeloid cells transiently during early development; whereas the second or adult wave of myelopoiesis emerges later from HSCs and produces myeloid cells continually during fetal and adult life. In the past decades, a great deal has been learnt about the development of myeloid cells from adult myelopoiesis, yet the genetic network governing embryonic myelopoiesis remains poorly defined. In this report, we present an in vivo study to delineate the role of Cebpa during zebrafish embryonic myelopoiesis. We show that embryonic myelopoiesis in cebpa-deficient zebrafish mutants initiates properly but fails to produce macrophages and neutrophils. The lack of macrophages and neutrophils in the mutants is largely attributed to the cell cycle arrest of embryonic myeloid progenitors, resulting in the impairment of their maintenance and subsequent differentiation. We further show that Cebpa, perhaps acting cooperatively with Runx1, plays a critical role in embryonic neutrophil maintenance. Our findings reveal a new role of Cebpa in embryonic myelopoiesis.

关键词： Cebpa Embryonic myelopoiesis Development Zebrafish