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Comparative transcriptome analysis between rhesus macaques(Macaca mulatta) and crab-eating macaques(M. fascicularis)

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Zoological Research 2024年第2期45卷 299-310页

作者： Yu-Xiang Mao Yamei Li zikun yang Ning Xu Shilong Zhang Xuankai Wang Xiangyu yang Qiang Sun Yafei Mao Institute of Neuroscience State Key Laboratory of NeuroscienceCenter for Excellence in Brain Science&Intelligence TechnologyChinese Academy of SciencesShanghai 200031China Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology Shanghai 201210China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Genetic Evolution&Animal Models Chinese Academy of SciencesKunmingYunnan 650201China Bio-X Institutes Key Laboratory for the Genetics of Developmental and Neuropsychiatric DisordersMinistry of EducationShanghai Jiao Tong UniversityShanghai 200030China Zhiyuan College Shanghai Jiao Tong UniversityShanghai 200240China Center for Genomic Research International Institutes of MedicineFourth Affiliated HospitalZhejiang UniversityYiwuZhejiang 322000China

Understanding gene expression variations between species is pivotal for deciphering the evolutionary diversity in phenotypes. Rhesus macaques(Macaca mulatta, MMU)and crab-eating macaques(M. fascicularis, MFA) serve as crucial nonhuman primate biomedical models with different phenotypes. To date, however, large-scale comparative transcriptome research between these two species has not yet been fully explored. Here, we conducted systematic comparisons utilizing newly sequenced RNA-seq data from84 samples(41 MFA samples and 43 MMU samples)encompassing 14 common tissues. Our findings revealed a small fraction of genes(3.7%) with differential expression between the two species, as well as 36.5% of genes with tissue-specific expression in both macaques. Comparison of gene expression between macaques and humans indicated that 22.6% of orthologous genes displayed differential expression in at least two tissues. Moreover,19.41% of genes that overlapped with macaque-specific structural variants showed differential expression between humans and macaques. Of these, the FAM220A gene exhibited elevated expression in humans compared to macaques due to lineage-specific duplication. In summary,this study presents a large-scale transcriptomic comparison between MMU and MFA and between macaques and humans. The discovery of gene expression variations not only enhances the biomedical utility of macaque models but also contributes to the wider field of primate genomics.

关键词： Crab-eating macaques Rhesus macaques Comparative transcriptomics Biomedical models Nonhuman primates RNA-seq Duplicated genes

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Smart Breeding Platform:A web-based tool for high-throughput population genetics,phenomics,and genomic selection

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Molecular Plant 2024年第5期17卷 677-681页

作者： Huihui Li Xin Li Peng Zhang Yingwei Feng Junri Mi Shang Gao Lele Sheng Mohsin Ali zikun yang Liang Li Wei Fang Wensheng Wang Qian Qian Fei Gu Wenbin Zhou State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural Sciences(CAAS)Beijing 100081China Nanfan Research Institute CAASSanyaHainan 572024China DAMO Academy Alibaba GroupHangzhou 310023China Hupan Lab Hangzhou 310023China Yazhouwan National Laboratory No.8 Huanjin RoadYazhou DistrictSanya CityHainan Province 572024China State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhouZhejiang 310006China

Dear Editor,In the era of big data and artificial intelligence,"smart breeding"has become a broad conceptual framework encompassing the paradigm shift of crop breeding to relying on analysis of high-throughput population genetics and phenomics data to conduct genomic selection,allowing identification and optimal use of the genetic potential in crop species(Xiao et al.,2022;Xu et al.,2022;Wang et al.,2023).Most existing tools for analyzing high-throughput breeding data require extensive computational power,complex installation processes,and command-line expertise and are therefore challenging and inconvenient for the majority of researchers and breeders(Brandies and Hogg,2021).

关键词： breeding artificial breed

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Study of Underground River Tracing System Based on SINS

Study of Underground River Tracing System Based on SINS

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第37届中国控制会议

作者： Hongwei Li Zhihua Li Fan Li zikun yang Pei Gao Wenqi Zhou School of Automation China University of Geosciences Hubei Key Laboratory of Advanced Contol and Intelligent Automation for Complex Systems

Underground rivers are underground flowing water that develop in carbonate areas or in water enrichment formations. At present, the underground river detection methods mainly include isotope tracer method, magnetotelluric method, and controllable source audio magnetotelluric method. In this paper, according to consulting a large amount of literature data, a new method of underground river tracing based on strapdown inertial navigation technology is proposed. The inertial device is installed on a floating carrier and the carrier is drifted with river water. The acceleration and angular velocity of the carrier are recorded, and the trajectory of the carrier is calculated by the attitude algorithm, then obtaining a concrete trace of the underground river. This method overcomes the disadvantages of the traditional measurement methods that are greatly affected by the environment and difficult to measure.

关键词： Underground river inertial navigation attitude solving algorithm

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Application of pseudo random correlation identification method based on LabVIEW in electromagnetic prospecting

Application of pseudo random correlation identification meth...

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第37届中国控制会议

作者： Fan Li ZhiHua Li Hongwei Li zikun yang Pei Gao Wenqi Zhou School of Automation China University of Geosciences Hubei Key Laboratory of Advanced Contol and Intelligent Automation for Complex Systems

Electromagnetic exploration plays an important role in the development of mineral, oil and gas resources and other minerals. With the improvement of exploration depth and longitudinal resolution, as well as the increasing humanistic interference in modern society, the electromagnetic exploration method put forward higher requirements. Improving the signal to noise ratio is the key to the development of electromagnetic exploration. Since the pseudo-random sequence is proposed as an artificial field source in the exploration of electromagnetic field, the pseudo-random technique based on correlation identification is also applied to the exploration of electromagnetic method in view of the good correlation characteristics and anti-jamming ability of pseudo-random sequence. In this paper, the principle of correlation identification is introduced, and the realization of correlation spectrum analysis in Lab VIEW is studied. The 2 n sequence pseudorandom signal is used as system excitation signal to verify the anti-jamming performance of correlation identification method in electromagnetic prospecting. It has great application prospect.

关键词： Related identification LabVIEW Spectral estimation Electromagnetic prospecting

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Design of Distributed Three Component Seismic Data Acquisition System Based on LoRa Wireless Communication Technology

Design of Distributed Three Component Seismic Data Acquisiti...

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第37届中国控制会议

作者： Pei Gao Zhihua Li Fan Li Hongwei Li zikun yang Wenqi Zhou School of Automation China University of Geosciences Hubei Key Laboratory of Advanced Control and Intelligent Automation for Complex Systems

The traditional wired seismograph has disadvantages such as cumbersome cable, complicated wiring, crosstalk and noise between adjacent transmission lines. In addition, the dynamic range of the instrument is small, and the observation of seismic signals is not intuitive, which can not meet the needs of high-precision seismic exploration. In order to solve these problems, the design of distributed three component seismic data acquisition system based on Lo Ra wireless communication technology is proposed. The system uses Lo Ra long-distance wireless communication technology and 24 bit Σ-Δ ADC, which realizes long-distance wireless communication and large dynamic range seismic data acquisition. Besides, the system has manmachine interaction interface, which can observe the parameters of seismic waveform in real time and adjust sampling rate directly. The whole circuit has the advantages of simple design, high precision and good stability. To sum up, the design has certain practical value and application prospect.

关键词： Seismic data acquisition system LoRa technology Human-computer interaction Σ-Δ ADC

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Research on diurnal variation of seabed magnetic field

Research on diurnal variation of seabed magnetic field

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第37届中国控制会议

作者： zikun yang Zhihua Li Fan Li Hongwei Li Pei Gao Wenqi Zhou School of Automation China University of Geosciences

Marine magnetic measurement survey is an important method of marine geophysical exploration. Geomagnetic diurnal change is an important part of marine magnetic survey. However, magnetic measurements in the far sea area are often caused by the lack of daily observation data near the survey area. The results are not properly corrected, in the deep water area layout of the submarine magnetic field observation system is one of the better ways to solve the problem. In this paper, the key technical problems such as low power consumption design and system recovery in submarine geomagnetic daily observation system are analyzed and studied, and the design scheme of submarine geomagnetic daily observation system is given. Experiments show that the new submarine geomagnetic daily observation system can meet the needs of magnetic field measurement.

关键词： Submarine magnetic field Low power consumption System recovery Magnetic field measurement

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年金保险危险选择策略研究

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保险职业学院学报 2010年第4期24卷 16-17页

作者：杨子昆中国人寿保险集团公司北京100000

本文对年金保险危险选择保单挂起时点从最优、战略和实际三方面提出了解决方法,进行了学术探讨。

关键词：年金保险危险选择研究

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水电开发及其对水文水资源特性的潜在影响——以云南省为例

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云南师范大学学报（哲学社会科学版） 2009年第3期41卷 37-41页

作者：黄英杨自坤刘新有云南省水文水资源局云南昆明650106

水文水资源特性的变化是影响河流生态健康及水资源开发利用的关键性因素。云南水能资源丰富且开发条件好,随着水电开发步伐的加快,水电开发对河流水文水资源特性的影响将进一步增大。从云南自然地理与水能资源特征出发,调查云南六大流... 详细信息

水文水资源特性的变化是影响河流生态健康及水资源开发利用的关键性因素。云南水能资源丰富且开发条件好,随着水电开发步伐的加快,水电开发对河流水文水资源特性的影响将进一步增大。从云南自然地理与水能资源特征出发,调查云南六大流域水电开发规划与开发现状,分析云南水电开发对河流径流、泥沙以及水文测验的潜在影响,可为云南电水开发影响客观评价的提供重要依据,有助于提出科学的应对措施。

关键词：水电开发径流泥沙水文测验六大流域

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转Bt基因南林895杨对美国白蛾抗虫性研究

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中南林业科技大学学报 2020年第7期40卷 107-118页

作者：孙伟博王璞杨梓堃李红岩诸葛强南京林业大学生物与环境学院江苏南京210037

【目的】通过对转Bt基因南林895杨进行虫试测定,分析转基因植物对不同世代美国白蛾Hyphantria cunea的杀虫活性、转基因杨树抗虫效果的时空动态、转基因杨树对不同龄期美国白蛾的杀虫效果,以及对美国白蛾幼虫生长发育的影响,为转基因杨... 详细信息

【目的】通过对转Bt基因南林895杨进行虫试测定,分析转基因植物对不同世代美国白蛾Hyphantria cunea的杀虫活性、转基因杨树抗虫效果的时空动态、转基因杨树对不同龄期美国白蛾的杀虫效果,以及对美国白蛾幼虫生长发育的影响,为转基因杨树的应用推广和美国白蛾防治提供相关依据。【方法】以实验室获得的转Bt(Cry1Ah1)基因南林895杨16个株系为材料,对美国白蛾幼虫进行室内喂养和田间套笼饲虫实验,实验分别采用不同世代、不同虫龄的美国白蛾幼虫为实验对象,测量其死亡率、化蛹率及取食量等指标,分析转Bt基因杨树对于美国白蛾生长的实际影响,并选取不同部位叶片分析杀虫效果的空间变化。【结果】16个转Bt基因南林895杨株系中,A-4-6、A-5-0、A-5-23和X-2-7株系具有良好的杀虫效果,幼虫取食叶片12 d后的校正死亡率为37.5%~68.9%。A-4-6株系的毒蛋白表达量最高达到11500.01 ng/g,占总蛋白表达量的0.0478%。其余5个杀虫活性较好株系的毒蛋白表达量均大于4669.38 ng/g,除Z-1-3株系,各株系毒蛋白量占总蛋白量均超过0.02%。抗虫效果明显的株系上部叶片的抗虫效果优于中部叶片,下部叶片的抗虫效果差。不同虫龄比对结果表明低龄幼虫的校正死亡率显著高于高龄幼虫(P<0.05),而化蛹率随着龄期的升高而升高,幼虫对杀虫性的敏感度随着龄期的升高逐渐下降。【结论】转基因南林895杨中Bt基因的表达能够有效的抑制美国白蛾的生长,并具有时空特异性,其中A-4-6、A-5-0及A-5-23三个株系具有良好的杀虫效果,适用于作为抗虫杨树树种进行推广。

关键词： Bt 南林895杨美国白蛾抗虫性

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首都机场T3航站楼施工工地灭鼠效果评价

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中国国境卫生检疫杂志 2006年第5期29卷 301-302页

作者：田洁曹建中杨自昆首都机场出入境检验检疫局北京100621 首都机场爱卫会

〔目的〕摸清T3航站楼施工工地的鼠密度本底情况,收集媒介生物控制基础资料,指导今后的灭鼠工作。〔方法〕2005年4￣5月,首都机场出入境检验检疫局在投药灭鼠前后,采用夹夜法对首都机场口岸T3航站楼施工工地的鼠密度进行了调查。〔结果... 详细信息

〔目的〕摸清T3航站楼施工工地的鼠密度本底情况,收集媒介生物控制基础资料,指导今后的灭鼠工作。〔方法〕2005年4￣5月,首都机场出入境检验检疫局在投药灭鼠前后,采用夹夜法对首都机场口岸T3航站楼施工工地的鼠密度进行了调查。〔结果〕4月,投药灭鼠前,鼠密度为5.33%。5月,在采取以投药为主的综合防治措施后,T3航站楼施工工地鼠密度下降至0.33%,灭鼠率为93.80%。〔结论〕以投药为主的综合防治措施较为有效,取得了良好的灭鼠效果。

关键词：鼠科航站楼灭鼠效果评价

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