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Artificial cilia for soft and stable surface covalent immobilization of bone morphogenetic protein-2

Bioactive Materials 2023年第6期24卷 551-562页

作者： Qi Gan Lina Chen Ho-Pan Bei Sze-Wing ng Han Guo Guoqiang Liu Hao Pan Changsheng Liu Xin zhao zijian zh.ng Laboratory for Advanced Interfacial Materials and Devices School of Fashion and TextilesThe Hong Kong Polytechnic UniversityKowloon99077Hong Kong Special Administrative Region of China Key Laboratory for Ultrafine Materials of Ministry of Education School of Materials Science and EngineeringEast China University of Science and TechnologyShanghai200237PR China Department of Biomedical Engineering The Hong Kong Polytechnic UniversityHung HomHong Kong Special Administrative Region of China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai201204PR China Department of Applied Biology and Chemical Technology Faculty of ScienceThe Hong Kong Polytechnic UniversityKowloon99077Hong Kong Special Administrative Region of China

Preservation of growth factor sensitivity and bioactivity(e.g.,bone morphogenetic protein-2(BMP-2))post-immobilization to tissue engineering scaffolds remains a great ***,we develop a stable and soft surface modification strategy to address this ***-2(a model growth factor)is covalently immobilized onto homogeneous poly(glycidyl methacrylate)(PGMA)polymer brushes which are grafted onto substrate surfaces(Au,quartz glass,silica wafer,or common biomaterials)via surface-initiated atom transfer radical *** surface modification method multiplies the functionalized interfacial area;it is simple,fast,gentle,and has little effect on the loaded protein owing to the cilia *** immobilized BMP-2(i-BMP-2)on the surface of homogeneous PGMA polymer brushes exhibits excellent bioactivity(-87%bioactivity of free BMP-2 in vitro and 20%-50%higher than scaffolds with free BMP-2 in vivo),with conformation and secondary structure well-preserved after covalent immobilization and ethanol ***,the osteogenic activity of i-BMP-2 on the nanoline pattern(PGMA-poly(N-isopropylacrylamide))shows-110%bioactivity of free *** is superior compared to conventional protein covalent immobilization strategies in terms of both bioactivity preservation and therapeutic *** polymer brushes can be used to modify surfaces of different tissue-engineered scaffolds,which facilitates in situ immobilization of growth factors,and accelerates repair of a wide range of tissue types.

关键词： Polymer brush Poly(glycidyl methacrylate) Surface modification Protein immobilization Bioactivity

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Determination of estrogens and estrogenic activities in water from three rivers in Tianjin, China

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Journal of Environmental Sciences 2013年第6期25卷 1164-1171页

作者： Kaifeng Rao Bingli Lei Na Li Mei Ma zijian Wang Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Institute of Environmental Pollution and Health College of Environmental and Chemical EngineeringShanghai University Key Laboratary for Biomedical Effects of Nanomaterials and Nanosafety Key Laboratory of Nuclear Analytical TechniquesInstitute of High Energy PhysicsChinese Academy of Sciences

Studies on estrogenic disrupting compounds （EDCs） occurrence and identification of main responsible compounds in river water discharged into the sea are of significance. In the present research, we screened estrogenic activities of 10 river water samples from 3 main rivers discharged into Bohai Sea in Tianjin using a recombinant two-hybrid yeast assay and chemical analysis by gas chromatography-mass spectrometry. All sample extracts induced significant estrogenic activity, with 17^-estradiol equivalents （EEQ） of raw water ranging from 5.72 to 59.06 ng/L. Six most concerned EDCs in the fiver water samples including estrone, 17（5-estradiol, 17ct-ethinylestradiol, estriol, diethylstilbestrol and estradiol valerate were determined, with their concentrations up to 50.70, 31.40, 24.40, 37.20, 2.56, and 8.47 ng/L, respectively. Through causality analysis by comparing the EEQ values of yeast assay and chemical analysis, 17ct-ethinylestradiol and 17[3-estradiol were identified as the main contributors to the estrogenic effects of the river samples, accounting for the whole estrogenic activities （62.99% to 185.66%）, and estrogen antagonistic compounds might presented in the heavy polluted water samples. The proposed approach using both chemical analysis and bioassay could be used for identification and evaluation of the estrogenic activity of EDCs in river water.

关键词： river water endocrine disrupting compounds (EDCs) yeast assay bioassay

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Assessment of source water contamination by estrogenic disrupting compounds in China

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Journal of Environmental Sciences 2012年第2期24卷 320-328页

作者： Weiwei Jiang YeYan Mei Ma Donghong Wang Qian Luo zijian Wang Senthil Kumaran Satyanarayanan Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China Unit of Toxicology Bharathiar University Coimbatore 641046 lndia

Detection of estrogenic disrupting compounds（EDCs） in drinking waters around China has led to rising concerns about health risks associated with these *** is,however,a paucity of studies on the occurrence and identification of the main compounds responsible for this pollution in the source *** fill this void,we screened estrogenic activities of 23 source water samples from six main river systems in China,using a recombinant two-hybrid yeast *** sample extracts induced significant estrogenic activity,with E2 equivalents（EEQ） of raw water ranging from 0.08 to 2.40 ng/***,16 samples were selected for chemical analysis by gas chromatography-mass *** EDCs of most concern,including estrone（E1）,17β-estradiol（E2）,17α-ethinylestradiol（EE2）,estriol（E3）,diethylstilbestrol（DES）,estradiol valerate（EV）,4-t-octylphenol（4-t-OP）,4-nonylphenols（4-NP） and bisphenol A（BPA）,were determined at concentrations of up to 2.98,1.07,2.67,4.37,2.52,1.96,89.52,280.19 and 710.65 ng/L,*** analysis,involving comparison of EEQ values from yeast assay and chemical analysis identified E2,EE2 and 4-NP as the main responsible compounds,accounting for the whole estrogenic activities（39.74% to 96.68%）.The proposed approach using both chemical analysis and yeast assay could be used for the identification and evaluation of EDCs in source waters of China.

关键词： source water estrogenic disrupting compounds yeast assay bioassay

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Simultaneous determination of ten taste and odor compounds in drinking water by solid-phase microextraction combined with gas chromatography-mass spectrometry

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Journal of Environmental Sciences 2013年第11期25卷 2313-2323页

作者： Xichao Chen Qian Luo Shengguang Yuan Zi Wei Hanwen Song Donghong Wang zijian Wang State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences

Taste and odor （T＆O） problems in drinking water frequently occur because of many compounds present in the water, of which trans-1,10-dimethyl-trans-9-decalol （geosrnin） and 2-methylisoborneol （MIB） are well-known. In this study, a fast and effective method was established for simultaneous determination of 10 T＆O compounds, including geosmin, MIB, 2,4,6-trichloroanisole （TCA）, 2-methylbenzofuran, 2-isopropyl-3-methoxypyrazine （IPMP）, 2-isobutyl-3-methoxypyrazine （IBMP）, cis-3-hexenyl acetate, trans,trans-2,4-heptadienal, trans, cis-2,6-nonadienal, and trans-2-decenal in water samples by headspace solid-phase microextraction （SPME） coupled with gas chromatography-mass spectrometry. An orthogonal array experimental design was used to optimize the effects of SPME fiber, extraction temperature, stirring rate, NaC1 content, extraction time, and desorption time. The limits of detection ranged from 0.1 to 73 ng/L were lower than or close to the odor threshold concentrations （OTCs）. All the 10 T＆O compounds were detected in the 14 water samples including surface water, treatment process water and tap water, taken from a waterworks in Lianyungang City, China. MIB and geosmin were detected in most samples at low concentration. Six T＆O compounds （IPMP, IBMP, trans,cis-2,6-nonadienal, 2-methylbenzofuran, trans-2-decenal, and TCA） were effectively decreased in water treatment process （sedimentation and filtration） that is different from cis-3-hexenyl acetate, MIB and geosmin. It is noted that the TCA concentrations at 15.9-122.3 ng/L and the trans,cis-2,6-nonadienal concentrations at 79.9-190.1 ng/L were over 10 times higher than their OTCs in tap water. The variation of the analytes in the all water samples, especially distribution system indicated that distribution system cannot be ignored as a T＆O compounds source.

关键词： taste and odor compound drinking water SPME-GC,rMS geosmin 2,4,6-trichloroanisole

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Evaluation of endocrine disruption and dioxin-like effects of organic extracts from sewage sludge in autumn in Beijing, China

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Frontiers of Environmental Science & Engineering 2014年第3期8卷 433-440页

作者： Cao LIU Yiping XU Mei MA Bingbin HUAng Jingdong WU Qingyi MEng zijian WAng Robert Alan GEARHEART State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China Beijing Water Science and Technology Institute Beijing 100048 China Environmental Engineering Department Humboldt State University Arcata CA95521 USA

Study on effective disposal and utilization of sewage sludge has recently been the target of growing interest in China. However, potential risks are associated with the use of sludge due to its contamination with toxic organics, heavy metals and pathogenic microorganisms. In this study, a screening assessment was conducted on sewage sludge samples collected from 17 different sewage treatment plants in Beijing, based on a batch of in vitro bioassays, including a set of recombinant gene yeast assays for endocrine disruption, and an ethoxy resorufin-O- deethylase （EROD） assay using H4IIE cells for aryl hydrocarbon receptor （Ah-R） agonistic activities. Our results suggested that moderate levels of estrogen receptor agonistic activities （0.9 ng E2. g-1 to 6.8 ng E2. gl, dw）, but relative higher androgen receptor antagonistic activ- ities （nd to 45%）, progestin receptor antagonistic activities （nd to 80%） and Ah-R agonistic activities （1390 to 6740 pg 2,3,7,8-tetrachlorodibenzo-p-dioxin （TCDD）·g-1, dw） were found in sewage sludge samples. However, there were no significant correlations between the toxic effects of sewage sludge and the sewage treatment processes. In addition, the 17β-estradiol （E2） equivalent quantity （EEQ） level of the sewage sludge was increased after the composting treatment, whereas the 2,3,7,8-tetrachlorodi- benzo-p-dioxin toxic equivalent quantity （TEQ） level of sewagesludge composted was much lower than that of sewage sludge.

关键词： sewage sludge in vitro bioassay endocrine disruption dioxin-like effect

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A gas chromatography/mass spectrometry method for the simultaneous analysis of 50 phenols in wastewater using deconvolution technology

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Chinese Science Bulletin 2011年第3期56卷 275-284页

作者： zh.ng WenJue WAng DongHong XU XiaoWei WAng BingYi LUO Qian SENTHIL KUMARAN Satyanarayanan WAng zijian State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China Unit of Toxicology Bharathiar University Coimbatore 641046 India

Phenolic compounds exist widely in the influents and effluents of sewage treatment plants (STPs) and most are *** this study,a gas chromatography-mass spectrometry (GC-MS) method for the simultaneous analysis of 50 phenolic compounds in wastewater was *** technology was used to identify contaminants that are covered by co-extracted materials.A mass spectral library containing all 50 silylated phenolic compounds was first established and used for *** typical phenolic compounds were selected to optimize the sample preparation ***-phase extraction using a C18 cartridge coupled with an HLB cartridge was used for pre-concentration and dichloromethane was used for *** solutes were derivatized and analyzed by *** blank and matrix spike recoveries ranged from 57.46% to 136.4% and 47.87% to 114.8%,*** detection limits ranged from 3.64 to 97.64 ng L–*** relative standard deviations of all the recovery experiments were lower than 13.6%.The instrument limits of quantification ranged from 0.7 to 87.7 *** method has been applied to analyze the influents and effluents of 5 Chinese *** for regulated phenolic compounds (phenol and 2,4,6-trichlorophenol),three un-regulated phenolic compounds,including 2-chlorophenol,2,5-dichlorophenol and 2,4-dichloro3-ethyl-6-nitrophenol were identified in the effluent *** detected concentrations of un-regulated phenolic compounds could possibly cause environmental effects,indicating that immediate attention is required to prevent complications.

关键词：酚类化合物废水利用气相色谱/质谱法解卷积时分技术污水处理厂气相色谱质谱法

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固相萃取-高效液相色谱串联质谱法同时测定鱼子酱中12种磺胺类药物残留

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质量安全与检验检测 2024年第1期34卷 25-31页

作者：陈玉娇江利兵谢俊郑婷朱子健许源衢州海关浙江衢州324003

首次建立了一种同时测定鱼子酱中磺胺嘧啶、磺胺二甲基嘧啶、磺胺二甲基异恶唑和磺胺间甲氧嘧啶等12种磺胺类药物残留的固相萃取-高效液相色谱-串联质谱分析方法。根据鱼子酱脂溶性的特点,试样采用正己烷溶剂预溶解,用乙酸乙酯提取2次... 详细信息

首次建立了一种同时测定鱼子酱中磺胺嘧啶、磺胺二甲基嘧啶、磺胺二甲基异恶唑和磺胺间甲氧嘧啶等12种磺胺类药物残留的固相萃取-高效液相色谱-串联质谱分析方法。根据鱼子酱脂溶性的特点,试样采用正己烷溶剂预溶解,用乙酸乙酯提取2次后经混合型阳离子交换固相萃取柱(MCX)净化,用InertSustain Swift C_(18)柱(150 mm×4.6 mm,5μm)分离,多反应监测(MRM)模式串联质谱进行测定,以内标法定量。结果显示,磺胺类药物在1.00~20.00 ng/mL范围内12种测定物均具有较好的线性关系,相关系数均≥0.99,方法定量限(S/N>10)为1.0μg/kg。对阴性鱼子酱在3个添加水平下(1.0μg/kg、2.0μg/kg和5.0μg/kg)分别进行加标回收试验,实际样品中12种测定物质的平均回收率在72.9%~119.0%之间,相对标准偏差(RSD,n=6)在0.9%~11.0%之间。应用本方法对2018—2020年采集的320个鱼子酱样品进行检测,结果表明均为未检出磺胺类药物残留。本方法快速、灵敏、准确,适用于日常大批量鱼子酱样品中磺胺类药物及其代谢物残留的定性、定量分析。

关键词：鱼子酱固相萃取高效液相色谱-串联质谱磺胺类药物残留量

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HPLC梯度洗脱法测定缩宫素注射液的含量

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今日药学 2024年第7期34卷 513-516页

作者：黎珊珊黄子健卢瑞芯黎芷明朱翠娴吴映晖周小琴广州白云山明兴制药有限公司广东广州510250

目的建立缩宫素注射液含量的高效液相色谱方法。方法采用Welch Xtimate^®C_(18)(150 mm×4.6 mm,3μm)色谱柱,检测波长为220 nm;流速为1.0 m L·min^(-1),柱温为40℃。以乙腈-水-缓冲盐溶液(0.2 mol·L^(-1)无水磷酸二氢钠,用氢氧化... 详细信息

目的建立缩宫素注射液含量的高效液相色谱方法。方法采用Welch Xtimate^®C_(18)(150 mm×4.6 mm,3μm)色谱柱,检测波长为220 nm;流速为1.0 m L·min^(-1),柱温为40℃。以乙腈-水-缓冲盐溶液(0.2 mol·L^(-1)无水磷酸二氢钠,用氢氧化钠调p H值至6.2,15∶70∶15)为流动相A,以乙腈-水-缓冲盐溶液(0.2 mol·L^(-1)无水磷酸二氢钠,用氢氧化钠调p H值至6.2,70∶15∶15)为流动相B,采用梯度洗脱。结果空白溶剂和空白辅料溶液对缩宫素的测定无干扰;缩宫素的线性范围为2.007 8~20.077 8 IU·m L^(-1)(r=0.999 9);平均回收率为98.54%(RSD为1.34%);缩宫素的定量限及检测限分别为8.393 0 ng·m L^(-1)和2.517 9 ng·m L^(-1);供试品溶液在室温放置24 h稳定。结论本方法准确、简便、可靠,可用于缩宫素注射液的含量测定。

关键词：缩宫素注射液含量测定高效液相色谱法

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Toxicokinetics and the related metabolites in rainbow trout(Oncorhynchus mykiss) after exposure to decabromodiphenyl ether

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Science China Chemistry 2010年第11期53卷 2379-2386页

作者： FEng ChengLian,XU YiPing,zhA JinMiao,LUO Qian,SHAN XiaoQuan & WAng zijian State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing China

Decabromodiphenyl ether(BDE209) is poorly absorbed by mammals,and little information is available on the toxicokinetics of BDE209 and its metabolites in *** the present study,rainbow trout(Oncorhynchus mykiss) were administered to 100 ng/g and 500 ng/g body wet weight of BDE209 via a single intraperitoneal injection and parent BDE209 and its metabolites were sequentially monitored for 28 *** results showed that toxicokinetic profiles of BDE209 could be described by the one-compartment *** the higher dose group(500 ng/g wet weight),the calculated half-life(t1/2) and elimination rate(ke) were 17.7 d and 0.039/d in the liver,and 100.3 d and 0.007/d in the muscle,*** major methoxylated brominated diphenyl ethers(MeO-BDEs) were detected with 2,2',4,4'-tetrabromo-5-methoxydiphenyl ether(5-MeO-BDE47) being detected in all tissue *** was no significant temporal change of 5-MeO-BDE47 concentration in the muscle,whereas an exponential increase was observed in the ***,the metabolism rate of BDE209 depended on the administered ***209 was hardly accumulated in the muscle of rainbow trout,while the liver was a primary metabolic ***-BDEs were formed via metabolism of BDE209,which probably played a significant role in fish toxicology as a potential indicator.

关键词： decabromodiphenyl ether(BDE209) polybrominated diphenyl ethers(PBDEs) methoxylated brominated diphenyl ethers(MeO-BDEs) toxicokinetics rainbow trout

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Debrominated and methoxylated polybrominated diphenyl ether metabolites in rainbow trout(Oncorhynchus mykiss) after exposure to decabromodiphenyl ether

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Journal of Environmental Sciences 2010年第9期22卷 1425-1434页

作者： Chenglian Feng,Yiping Xu,Yue He,Qian Luo,Jinmiao zha,zijian Wang State Key Laboratory of Environmental Aquatic Chemistry,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China. State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China

Decabromodiphenyl ether （BDE209） is the primary component in a commonly used flame retardant. Previous studies had proved that BDE209 itself was not toxic, while its metabolites including debrominated diphenyl ethers （De-BDEs） and methoxylated brominated diphenyl ethers （MeO-BDEs） posed a potential threat to organisms. Many studies had indicated that BDE209 could metabolize quickly in mammals, but lacking in the basic data about the metabolism of BDE209 in fish. In the present study, two replicate treatment groups of rainbow trout （Oncorhynchus rnykiss） were exposed to BDE209 via a single intraperitoneal injection approximately 100 and 500 ng/g, respectively. Muscle, liver and blood samples were collected to analyze the specific metabolites on day 1 and day 28 post injection. The highest concentration of BDE209 was detected in muscle tissues, from 796.1 ng/g wet weight （day 1） to 687.1 ng/g wet weight （day 28） in high dose group, suggesting that BDE209 could accumulate slightly in muscle tissues. However, BDE209 was not detected in the blood for all treatments. Most congeners of De-BDEs were found in muscle and liver tissues, with the highest concentration in the liver. The main De-BDEs were nona-, octa-, hepta- and penta-De-BDEs. A total of seven MeO-BDE metabolites were observed among different fish tissues. Blood had the highest contribution of the MeO-BDE metabolites. Each MeO-BDE congener increased over the 28 days. These results in contrast to other studies suggested possible species-specific differences in metabolic abilities.

关键词： decabromodiphenyl ether (BDE209) methoxylated brominated diphenyl ethers (MeO-BDEs) debrominated diphenyl ethers (De-BDEs) rainbow trout

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