Determination of estrogens and estrogenic activities in water from three rivers in Tianjin, China

作     者：Kaifeng Rao Bingli Lei Na Li Mei Ma Zijian Wang 

作者机构：Research Center for Eco-Environmental SciencesChinese Academy of Sciences Institute of Environmental Pollution and HealthCollege of Environmental and Chemical EngineeringShanghai University Key Laboratary for Biomedical Effects of Nanomaterials and NanosafetyKey Laboratory of Nuclear Analytical TechniquesInstitute of High Energy PhysicsChinese Academy of Sciences 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2013年第25卷第6期

页      面：1164-1171页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 082803[工学-农业生物环境与能源工程] 08[工学] 0828[工学-农业工程] 0804[工学-仪器科学与技术] 

基　　金：supported by the National High Technology Research and Development Program (863) of China (No.2009AA06Z402) the National Science and Technology Major Project of China (No. 2008ZX07209-010) the National Natural Science Foundation of China (No.20921063) 

主　　题：river water endocrine disrupting compounds (EDCs) yeast assay bioassay 

摘      要：Studies on estrogenic disrupting compounds （EDCs） occurrence and identification of main responsible compounds in river water discharged into the sea are of significance. In the present research, we screened estrogenic activities of 10 river water samples from 3 main rivers discharged into Bohai Sea in Tianjin using a recombinant two-hybrid yeast assay and chemical analysis by gas chromatography-mass spectrometry. All sample extracts induced significant estrogenic activity, with 17^-estradiol equivalents （EEQ） of raw water ranging from 5.72 to 59.06 ng/L. Six most concerned EDCs in the fiver water samples including estrone, 17（5-estradiol, 17ct-ethinylestradiol, estriol, diethylstilbestrol and estradiol valerate were determined, with their concentrations up to 50.70, 31.40, 24.40, 37.20, 2.56, and 8.47 ng/L, respectively. Through causality analysis by comparing the EEQ values of yeast assay and chemical analysis, 17ct-ethinylestradiol and 17[3-estradiol were identified as the main contributors to the estrogenic effects of the river samples, accounting for the whole estrogenic activities （62.99% to 185.66%）, and estrogen antagonistic compounds might presented in the heavy polluted water samples. The proposed approach using both chemical analysis and bioassay could be used for identification and evaluation of the estrogenic activity of EDCs in river water.