检索结果-南通市图书馆

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

腐败的实验研究法及其研究进展

心理技术与应用 2024年第5期12卷 296-307页

作者：魏子晗魏李帆刘洪志教育部人文社会科学重点研究基地天津师范大学心理与行为研究院天津300387 天津师范大学心理学部天津300387 学生心理发展与学习天津市高校社会科学实验室天津300387 南开大学社会学院天津300350

通过对近年来采用实验研究方法考察腐败行为的内容加以梳理,并对不同实验研究方法的优势与不足进行对比,以期促进我国腐败行为实验研究的发展。腐败行为的实验研究法可分为腐败情境模拟法、腐败博弈范式和田野实验法三类,借助这些实验... 详细信息

通过对近年来采用实验研究方法考察腐败行为的内容加以梳理,并对不同实验研究方法的优势与不足进行对比,以期促进我国腐败行为实验研究的发展。腐败行为的实验研究法可分为腐败情境模拟法、腐败博弈范式和田野实验法三类,借助这些实验研究方法,可以考察多种因素对腐败行为的影响,并检验相关反腐机制的有效性。在未来研究中,实验研究法可为评估反腐政策治理效果,开发腐败测量工具,分析腐败动态规律,探究集体腐败等多个方面提供有力的证据支持。

关键词：腐败研究贿赂研究贪污研究实验研究法

开放教育考试数字化转型:内涵特征、逻辑框架及推进路径

在线全文

学校读者我要写书评

暂无评论

终身教育研究 2024年第2期35卷 63-70页

作者：李凤霞王小军魏子涵张伟冯余佳江苏开放大学信息化建设处江苏开放大学信息工程学院

教育数字化转型是教育改革的趋势,也是实现教育现代化的必由之路。本研究结合开放教育发展需求,明晰了开放教育考试数字化转型的内涵特征,基于政策要求、社会需求等多元文化环境浸润下所具有的外延特征。针对目前开放教育考试数字化转... 详细信息

教育数字化转型是教育改革的趋势,也是实现教育现代化的必由之路。本研究结合开放教育发展需求,明晰了开放教育考试数字化转型的内涵特征,基于政策要求、社会需求等多元文化环境浸润下所具有的外延特征。针对目前开放教育考试数字化转型普遍存在目标不明确、路径不清晰、过程方法缺失等共性问题和挑战,构建了开放教育考试数字化转型的逻辑框架,并结合业务流程域规划了开放教育考试数字化转型的实践路径,以此为开放教育考试改革提供理论与实践参考,助力高质量全民终身教育体系的建设。

关键词：教育数字化转型开放教育考试数字化转型学习型社会建设

Dual-Emission Carbonized Polymer Dots Combined with Metal Ions as a Single-Component Fluorescence Sensor Array for Pattern Recognition of Glycosaminoglycans

在线全文

学校读者我要写书评

暂无评论

Journal of Analysis and Testing 2023年第3期7卷 285-294页

作者： Ziyang Lin Wenbang Yu Rong Hu Ruixuan Hu zihan wei Min Zhang Jinhua Polytechnic Jinhua 321000China Rizhao Polytechnic Rizhao 276800China School of Chemistry and Molecular Engineering Shanghai Key Laboratory for Urban Ecological Processes and Eco-RestorationEast China Normal UniversityShanghai 200241China

Herein,a novel dual-emission fluorescence sensor array of carbonized polymer dots(CPDs)-Cu^(2+)has been proposed,in which CPDs were prepared by one-pot hydrothermal method and Cu^(2+)acting as a quencher was combined with CPDs by electrostatic *** negatively charged glycosaminoglycans(GAGs)bearing different hydrophilic groups showed variable binding affinities towards CPDs-Cu^(2+).Upon reacting with these GAGs,the different fluorescence response signals of CPDs-Cu^(2+)can be further differentiated by principal component analysis(PCA).The CPDs-Cu^(2+)sensor array,not only allows the identification of four similarly structured GAGs,but also realizes the discrimination of different concentrations of the same GAGs and their ***,the identification of GAGs in biological fluids can also be achieved using our proposed single-component sensor array,validating its application *** new strategy avoids multiple sensing probes,broadens the application of tongue-mimic sensor arrays and provides a viable idea for the development of single component sensing platforms.

关键词： CPDs GAGs Single-component Dual-emission Sensor array Biological fluids

基于UPLC-Q-TOF-MS/MS及网络药理学探讨半枫荷治疗类风湿性关节炎的有效成分和作用机制

在线全文

学校读者我要写书评

暂无评论

湖北民族大学学报（医学版） 2024年第1期41卷 18-24页

作者：魏子涵晏晨李开斌张丽艳魏美琪马四补贵州中医药大学贵州贵阳550025

目的利用高效液相色谱-四极杆飞行时间串联质谱法(UPLC-Q-TOF-MS/MS)分析半枫荷醇提物的主要成分,结合网络药理学进一步探讨半枫荷抗风湿性关节炎有效物质及作用机制。方法依据UPLC-Q-TOFMS/MS表征分析半枫荷醇提物的主要化学成分,结合P... 详细信息

目的利用高效液相色谱-四极杆飞行时间串联质谱法(UPLC-Q-TOF-MS/MS)分析半枫荷醇提物的主要成分,结合网络药理学进一步探讨半枫荷抗风湿性关节炎有效物质及作用机制。方法依据UPLC-Q-TOFMS/MS表征分析半枫荷醇提物的主要化学成分,结合Pubchem数据库、Swiss Target Prediction数据库预测半枫荷的作用靶点。利用GeneCards数据库、OMIM数据库、PharmGkb数据库获得疾病靶点,用Venny图绘制平台获得成分和疾病的交集靶点;通过String数据库和Cytoscape 3.9.0软件构建化合物-靶点相互网络、蛋白质-蛋白质相互作用(PPI)网络,并筛选核心靶点,利用David数据库对潜在的核心靶点进行基因本体(GO)功能和京都基因与基因组百科全书(KEGG)通路富集分析,并制作GO双侧柱状图、富集结果分析图。用Cytoscape 3.9.0软件构建“活性成分-靶点-疾病-通路”网络图与活性成分与潜在靶点的网络图。用PyMOL和AutoDock Vina软件进行分子对接。结果从半枫荷醇提物中鉴定84个成分,从数据库筛选出43种活性成分,对应561个潜在靶点,其中与类风湿性关节炎交集靶点55个;经PPI网络分析后,获取核心靶点10个:IL-6、TNF、IL-1β、ALB、CCR5、PTGS2、TLR4、MMP9、NFKB1、STAT1。经有效成分-靶点网络得药物有效成分5个:光甘草内酯、泽泻醇C-23-醋酸酯、藁本内酯、洋川芎内酯A、积雪草酸。主要涉及通路:TNF信号通路、IL-17信号通路、Toll样受体信号通路、C型凝集素受体信号通路、脂质和动脉粥样硬化。分子对接结果有较好的结合能力。结论通过结合UPLC-Q-TOF-MS/MS和网络药理学及分子对接的方法初步阐明了半枫荷是通过多成分、多靶点、多途径发挥抗类风湿性关节炎的作用,为半枫荷的进一步质量评价及药理活性的研究提供参考。

关键词： UPLC-Q-TOF-MS/MS 半枫荷类风湿性关节炎网络药理学分子对接 Toll样受体信号通路藁本内酯积雪草酸

Spindle and Kinetochore-associated Family Genes are Prognostic and Predictive Biomarkers in Hepatocellular Carcinoma

在线全文

学校读者我要写书评

暂无评论

Journal of Clinical and Translational Hepatology 2022年第4期10卷 627-641页

作者： Chenhui Cai Ying Zhang Xu Hu Sizhen Yang Jiawen Ye zihan wei Tongwei Chu Department of Orthopedics Xinqiao HospitalThird Military Medical University(Army Medical University)ChongqingChina

Background and Aims:Hepatocellular carcinoma(HCC)is one of the most frequent malignant *** and kinetochore-associated(SKA)family genes are essential for the maintenance of the metaphase plate and spindle checkpoint silencing during *** studies have indicated that dysregulation of SKA family genes induces tumorigenesis,tumor progression,and chemoresistance via modulation of cell cycle and DNA ***,the differential transcription of SKAs in the context of HCC and its prognostic significance has not been ***:Bioinformatics analyses were performed using TCGA,ONCOMINE,HCCDB,Kaplan-Meier plotter,STRING,GEPIA ***-PCR,western blot,and functional as-says were utilized for in vitro ***:We found remarkable upregulation of transcripts of SKA family genes in HCC samples compared with normal liver samples on bioinformatics analyses and in vitro ***-action analysis and enrichment analysis showed that SKA family members were mainly related to microtubule motor activity,mitosis,and cell ***-infiltration analysis showed a correlation of all SKA family genes with various immune cell subsets,especially T helper 2(Th2)***-scriptional levels of SKA family members were positively as-sociated with histologic grade,T stage,andα-fetoprotein in HCC *** operating characteristic curve analy-sis demonstrated a strong predictive ability of SKA1/2/3 for *** expression of these SKAs was associated with unfavorable overall survival,progression-free survival,and disease-specific *** Cox proportional hazards regression analyses,SKA1 upregulation and pathological staging were independent predictors of overall survival and disease-specific survival of HCC ***,clinical tissue microarray validation and in vitro functional assays revealed SKA1 acts an important regulatory role in tumor malignant ***:SKA family members may potentially serve

关键词： Spindle and kinetochore-associated genes Liver hepatocellular carcinoma Prognostic value Immune infiltration Bioinformatics analysis

Gene variations of glutamate metabolism pathway and epilepsy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Epileptologica 2022年第2期5卷 105-119页

作者： Yan Feng Cuirong Zhang zihan wei Guoyan Li Yajing Gan Chao Liu Yanchun Deng Xi’an Medical University Xi’an710021China Department of Neurology Epilepsy Center of Xijing HospitalFourth Military Medical UniversityXi’an710032China Department of Neurology QinHuang HospitalXi’an710600China

Background Epilepsy is a paroxysmal disorder of the brain,caused by an imbalance of neuronal excitation and *** is the most important excitatory neurotransmitter in the brain and plays an important role in *** in genes at any step/component of the glutamate metabolic pathway may lead to the development of epilepsy or epileptic *** Clinical history of 3 epilepsy patients with genetic variations of the glutamate metabolism pathway was *** recording and magnetic resonance imaging were performed in each *** also reviewed recent literature for a variety of the genetic variations involved in *** Case 1 was a SLC1A2 mutation-carrier diagnosed with developmental and epileptic encephalopathy(DEE)41,whose seizures decreased after start of the ketogenic *** 2 carried a GRIN2A gene mutation and was seizure-free for three years after taking levetiracetam and vitamin *** 3 was a GRIN2B mutation-carrier diagnosed with DEE 27,who seizures diminished after taking *** Preclinical and clinical evidence supports the therapeutic potential of glutamatergic signaling-targeting treatments for *** studies are needed to discover novel DEE-related genetic mutations in the glutamate metabolic pathway.

关键词： Glutamate Epilepsy Developmental and epileptic encephalopathy

Disulfiram:A novel repurposed drug for cancer therapy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 2024年第12期137卷 1389-1398页

作者： Min Zeng Baibei Wu Wenjie wei zihan Jiang Peiqiang Li Yuanting Quan Xiaobo Hu Department of Biochemistry and Molecular Biology School of Basic MedicineHengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China The Key Laboratory of Ecological Environment and Critical Human Diseases Prevention of Hunan Province Department of Education Hengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China Institute of Biochemistry of Hengyang Medical School University of South ChinaHengyangHunan 421001China

Cancer is a major global health *** therapeutic strategies can prolong patients’survival and reduce the costs of *** repurposing,which identifies new therapeutic uses for approved drugs,is a promising approach with the advantages of reducing research costs,shortening development time,and increasing efficiency and ***(DSF),a Food and Drug Administration(FDA)-approved drug used to treat chronic alcoholism,has a great potential as an anticancer drug by targeting diverse human *** studies show the antitumor effects of DSF,particularly the combination of DSF and copper(DSF/Cu),on a wide range of cancers such as glioblastoma(GBM),breast cancer,liver cancer,pancreatic cancer,and *** this review,we summarize the antitumor mechanisms of DSF/Cu,including induction of intracellular reactive oxygen species(ROS)and various cell death signaling pathways,and inhibition of proteasome activity,as well as inhibition of nuclear factor-kappa B(NF-κB)***,we highlight the ability of DSF/Cu to target cancer stem cells(CSCs),which provides a new approach to prevent tumor recurrence and ***,DSF/Cu inhibits several molecular targets associated with drug resistance,and therefore it is becoming a novel option to increase the sensitivity of chemo-resistant and radio-resistant *** of DSF/Cu may shed light on its improved application to clinical tumor treatment.

关键词： Disulfiram Aldehyde dehydrogenase Reactive oxygen species Proteasome activity Cancer stem cells Drug resistance

在线全文

学校读者我要写书评

暂无评论

羊肚菌菌种出菇性状早期评价与田间验证

北京农学院学报 2024年第3期39卷 84-90页

作者：乔莹孔繁建左鑫陈青君张薇薇魏子涵曹博硕张国庆北京农学院植物科学技术学院农业应用新技术北京市重点实验室北京102206 北京市顺义区农业科学研究所北京101300

【目的】通过母种和原种生长特性、人工气候室出菇和田间栽培试验,建立羊肚菌菌种出菇性状早期评价体系,为栽培实践提供依据。【方法】以10个六妹羊肚菌商业菌种为材料,测定不同菌株母种和原种培养基菌丝生长速率、生物量等生长特性,观... 详细信息

【目的】通过母种和原种生长特性、人工气候室出菇和田间栽培试验,建立羊肚菌菌种出菇性状早期评价体系,为栽培实践提供依据。【方法】以10个六妹羊肚菌商业菌种为材料,测定不同菌株母种和原种培养基菌丝生长速率、生物量等生长特性,观测3种温度模式下人工气候室出菇过程中菌丝生长和菌核情况,记录田间栽培试验单菇鲜重、菌盖长度、菌盖直径、产量等农艺性状。【结果】各菌株在母种、原种和人工气候室出菇试验中存在显著差异,通过对三个试验进行分别赋分统计并求和,AK、G8和JYN菌株得分最高,表明具有潜在的高产能力。田间验证试验结果显示,AK、G8和JYN菌株在产量、单菇鲜重、子囊果长等农艺性状方面表现突出,与预测结果相符。【结论】利用母种和原种生长特性、人工气候室出菇表现,可以对羊肚菌菌种质量进行早期评价,为实践生产中菌种选择和质量评价提供技术支撑。

关键词：六妹羊肚菌生长特性原基密度田间栽培

梁漱溟乡村建设思想及其当代价值研究——基于A省D县的数字乡村建设实践

在线全文

学校读者我要写书评

暂无评论

云南农业大学学报（社会科学版） 2022年第3期16卷 56-63页

作者：魏子涵安徽大学社会与政治学院安徽合肥230601

梁漱溟提出的乡村建设思想,肯定了农村和农民对国家复兴的作用,强调通过社会组织再造、文化教育和多主体合作等途径解决乡村问题,对我国当前数字乡村建设具有巨大的价值。A省D县在数字乡村建设的实践过程中取得了阶段性成效,但同时也面... 详细信息

梁漱溟提出的乡村建设思想,肯定了农村和农民对国家复兴的作用,强调通过社会组织再造、文化教育和多主体合作等途径解决乡村问题,对我国当前数字乡村建设具有巨大的价值。A省D县在数字乡村建设的实践过程中取得了阶段性成效,但同时也面临着主体关系异化的问题。乡村建设思想的部分主张对整改现实问题、推进建设工作具有重要的理论指导作用。国家提出要"激活主体、激活要素、激活市场",既是实施数字乡村建设行动的三大路径,也是对梁漱溟乡村建设思想的当代价值的继承和印证。

关键词：梁漱溟乡村建设思想数字乡村建设当代价值