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Identification of four caspase genes from Spodoptera exigua (Lepidoptera: Noctuidae) and their regulations toward different apoptotic stimulations

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Insect Science 2020年第6期27卷 1158-1172页

作者： Huan Yu zi-qi li Yi-Yi Ou-Yang Guo-Hua Huang Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests Hunan Agricultural UniversityChangshaChina College of Plant Protection Hunan Agricultural UniversityChangshaChina

Apoptosis plays critical roles in multiple biological processes in multicellular *** are known as important participators and regulators of ***,four novel caspase genes of Spodoptera exigua were cloned and characterized which were designated as SeCasp-1,SeCasp-6,ScCasp-7 and *** of the putative encoded protein sequences of these SeCasps indicated that SeCasp-1 and ScCasp-7 were possible homologs of executor caspases;SeCasp-8 was a possible homolog of initiator caspases;and SeCasp-6 was a unique caspase of *** that shares low similarity with all the identified insect *** on baculovirus expression system analyses,SeCasp-1 exhibited similar caspase activity to human caspase-1,-3,-4,-6,-8 and-9;SeCasp-6 presented similar caspase activity to human caspase-2,-3,-4,-6,-8 and-9;SeCasp-7 exhibited similar caspase activity to human caspase-2,-3 and-6;and SeCasp-8 presented similar caspase activity only to human *** with diiTcrent chemicals revealed that SeCasp-1 showed extreme upregulation after 24 h in the treated fat body cell line(IOZCAS-Spex-II)of *** expression analysis revealed that SeCasp-1 was highly transcribed in the larval stages,while SeCasp-6,SeCasp-7,SeCasp-H were *** in vivo detection of the relative expression levels of SeCasps in *** larvae inoculated with different pathogens suggested that SeCasp-J was sensitive to Bacillus thuringiensis infection and that SeCasp-6 was sensitive to baculovirus ***-7 and SeCasp-H showed slight changes under either in vitro chemical apoptosis induction or in vivo pathogen infection.

关键词： apoptosis apoptotic inducing insect caspase SeCasp Spodoptera exigua

Melanization induced by Heliothis virescens ascovirus 3h promotes viral replication

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Insect Science 2021年第2期28卷 472-484页

作者： zi-qi li Xiao-Hui Song Min Wang Shu Wang Guo-Hua Huang Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests Hunan Agricultural UniversityChangshaChina College of Plant Protection Hunan Agricultural UniversityChangshaChina

Melanization is an important innate immune defense mechanism of insects,which can kill invading *** pathogens,for their survival and reproduction,inhibit the melanization of the ***,our results suggested that after infection with Heliothis virescens ascovirus 3h(HvAV-3h),the speed of melanization in infected Spodoptera exigiia larval hemolymph was accelerated and that the phenoloxidase(PO)activity of hemolymph in larvae infected with HvAV-3h increased significantly(1.20-fold at 96 hpi,1.52-fold at 120 hpi,1.23-fold at 144 hpi,1.12-fold at 168 hpi).The transcription level of the gene encoding *** prophenoloxidase-1(SePPO-1 gene)was upregulated dramatically in the fat body during the middle stage of *** addition,when melanization was inhibited or promoted,the replication of HvAV-3h was inhibited or promoted,*** conclusion,infection with HvAV-3h can markedly induce melanization in the middle stage of infection,and melanization is helpful for HvAV-3h viral replication.

关键词： Heliothis virescens ascovirus 3h melanization Spodoptera exigua viral replication

How to manage the malposition of deep vein catheterization into the artery?

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Journal of Geriatric Cardiology 2024年第6期21卷 682-685页

作者： Jun-Na SUN Hai DONG Peng CHEN zi-qi li li-You SUI Bin qi Quan-Min JING Department of Cardiovascular Diseases the First Affiliated Hospital of Henan University of Chinese MedicineZhengzhouChina Department of Cardiology General Hospital of Northern Theater CommandShenyangChina

Central venous catheterization establishes temporary,efficient,and rapid use of deep venous access in patients,which provides high flow rate fluid perfusion,enables measurement of central venous pressure,and acts as an important reference for clinical ***,various complications such as pneumothorax,hemothorax,hematoma,and puncture failure can easily occur during the puncture and catheterization process.

关键词： process enable catheter

Arthroplasty vs proximal femoral nails for unstable intertrochanteric femoral fractures in elderly patients: a systematic review and metaanalysis

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World Journal of Clinical Cases 2021年第32期9卷 9878-9888页

作者： Wen-Huan Chen Wen-Xuan Guo Shi-Hua Gao qiu-Shi Wei zi-qi li Wei He The Third Clinical Medical School Guangzhou University of Chinese MedicineGuangzhou 510405Guangdong ProvinceChina The First Clinical College Zhejiang Chinese Medical UniversityHangzhou 310000Zhejiang ProvinceChina The First Clinical Medical School Guangzhou University of Chinese MedicineGuangzhou 510405Guangdong ProvinceChina Department of Joint Surgery The Third Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510405Guangdong ProvinceChina

BACKGROUND Proximal femoral nails(PFNs)are the most common method for the treatment of unstable intertrochanteric femoral fractures(IFFs),but postoperative bed rest is *** is a large amount of blood loss during the *** in elderly patients may cause nonunion of fractures and other *** can give patients early weight bearing and reduce financial burden,but whether it can replace PFNs remains *** To compare the clinical outcomes of arthroplasty and PFNs in the treatment of unstable IFFs in elderly *** A search was conducted in the PubMed,Embase,and Cochrane library databases and included relevant articles comparing arthroplasty and *** search time was limited from January 1,2005 to November 1,*** investigators independently screened studies,extracted data and evaluated the quality according to the inclusion and exclusion *** to the research results,the fixed effect model or random effect model were selected for *** following outcomes were analyzed:Harris Hip score,mortality,complications,operation time,blood loos,hospital stay,weight-bearing time,fracture classification and type of *** We analyzed four randomized controlled trials that met the requirements.A total of 298 patients were included in these *** to the AO/OTA classification,there are 20 A1 types,136 A2 types,42 A3 types and 100 unrecorded *** outcome:The Harris Hip Score at the final follow-up of the PFN group was higher[mean difference(MD):9.01,95%confidence interval(CI):16.57 to 1.45),P=0.02].There was no significant difference between the two groups in the rate of overall mortality[risk ratio(RR):1.44,P=0.44]or the number of complications(RR:0.77,P=0.05).Secondary outcomes:blood loss of the arthroplasty group was higher(MD:241.01,95%CI:43.06–438.96,P=0.02);the operation time of the PFN group was shorter(MD:23.12,95%CI:10.46–35.77,P=0.0003);and the length of

关键词： Arthroplasty Proximal femoral nail Intertrochanteric femoral fracture Elderly Meta-analysis Systematic review

Novel strategies for the biocontrol of noctuid pests(Lepidoptera)based on improving ascovirus infectivity using Bacillus thuringiensis

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Insect Science 2021年第5期28卷 1452-1467页

作者： Huan Yu Chang-Jin Yang Ni li Ying Zhao Zhuang-Mei Chen Si-Jia Yi zi-qi li Michael J.Adang Guo-Hua Huang Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests Hunan Agricultural UniversityChangshaHunan410128China College of Plant Protection Hunan Agricultural UniversityChangshaHunan410128China Department of Entomology University of GeorgiaAthensGA30602USA

ldentifying novel biocontrol agents and developing new strategies are urgent goals in insect pest *** are potential competent insect viruses that may be developed into bioinsecticides,but this aim is impeded by their poor oral *** improve the per os infectivity of ascovirus,Bacillus thuringiensis kurstaki(Btk)was employed as a helper to damage the midgut of lepidopteran larvae(Helicoverpa armigera,Mythimna separata,Spodoptera frugiperda,and ***)in formulations with Heliothis virescens ascovirus isolates(HvAV-3h and HvAV-3j).Btk and ascovirus mixtures(Btk/HvAV-3h and B1k/HvAV-3j)were fed to insect larvae(3rd instar).With the exception of *** larvae,which exhibited low mortality after ingesting Btk,the larvae of the other tested species showed three types of response to feeding on the formulas:type I,the tested larvae(***)were killed by Btk infection so quickly that insufficient time and resources remained for ascoviral invasion;type II,both Btk and the ascovirus were depleted by their competition,such that neither was successfully released or colonized the tissue;type II,Btk was eliminated by the ascovirus,and the ascovirus achieved systemic infection in the tested *** feeding of Btk/ascovirus formulas led to a great reduction in larval diet consumption and resulted in a significant decrease in the emergence rate of ***,***,and *** larvae,which suggested that the formulas exerted marked oral control effects on both the contemporary individuals and the next generation of these tested pest species.

关键词： ascovirus Bacillus thuringiensis formulation Heliothis virescens ascovirus 3h(HvAV-3h) Heliothis virescens ascovirus 3j(HvAV-3j) per os infectivity

KNOEVENAGEL REACTION ON ZEOliTES

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Chinese Science Bulletin 1989年第10期34卷 832-837页

作者：陈子涛李齐芳 Department of Chemistry Nanjing University

Knoevenagel condensation of malonic acid with aldehyde or ketone is brought about by an organic base, such as pyridine, to give a-alkenyl carboxylic acid. Because the base must be separated and recovered, and the prod... 详细信息

关键词： Knoevenagel reaction zeolite synthesis α/β-alkenyl carboxylic acids.

Influence of Surrounding Acupuncture by Cranial MRI Location on Blood Rheology in Apoplectic Patients

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Journal of Acupuncture and Tuina Science 2003年第5期1卷 17-19页

作者： JIANG Gang-hui li Yan-hui ZHUANG zi-qi HUANG Yong li Jian-ping HUANG Guo-qi First Affiliated Hospital of Guangzhou University of TCM Guangzhou China 510405 First Affiliated Hospital of Guangzhou University of TCM Guangzhou China 510405 First Affiliated Hospital of Guangzhou University of TCM Guangzhou China 510405 First Affiliated Hospital of Guangzhou University of TCM Guangzhou China 510405 First Affiliated Hospital of Guangzhou University of TCM Guangzhou China 510405

Purpose: To study the function of surrounding acupuncture located by cranial MRI on ***: Forty cases of the patients diagnosed as cerebral infarction were divided into the group of surrounding acupuncture by MRI location and the group of traditional scalp acupuncture, 20 cases in each group, and were treated respectively with surrounding acupuneture by MRI location and traditional scalp acupuncture, to determine the indexes of blood rheology before and after ***: There was significant difference (Pling techniques have an improving function in various indexes of blood rheology in the apoplectic patients and surrounding acupuncture by MRI location was better than traditional scalp acupuncture in improving blood rheology. Key Words Acupuncture Therapy - Hemodynamics - Cerebral Infarction JIANG Gang-hui, associate professor, has mainly done the clinical study in treating diseases of brain and spinal cord with acupuncture.Translator: HUANG Guoqi

关键词： Acupuncture Therapy Hemodynamics Cerebral Infarction

Specifically targeting human telomeric dimeric G-quadruplexes by polyehter-tethered di-berberine derivatives

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Specifically targeting human telomeric dimeric G-quadruplexe...

Effect of Whole Body Vibration Therapy on Circulating Serotonin Levels in an Ovariectomized Rat Model of Osteoporosis

中国化学会第四届卟啉与酞菁学术研讨会

作者： zi-qi li Ting-Cong liao Wen-Hua Chen Chun-qiong Zhou Guangdong Provincial Key Laboratory of New Drug Screening School of Pharmaceutical SciencesSouthern Medical University

The design and synthesis of binders specifically multiple G-quadruplex units in the 3’-terminal overhang of human telomeric DNA has attracted considerable attention,which will promote to develop some selective anticancer agents with fewer side *** three polyether-tethered di-berberine compounds（1-3） have been prepared and studied on their binding affinity,selectivity and thermal stabilization towards dimeric quadruplex DNA by UV-Vis titrations,electrophoresis,fluorescence spectroscopy,CD spectroscopy and *** 1 with the shortest polyether linker has been found to have higher binding affinity,selectivity and thermal stabilization towards mixed-type dimeric quadruplexes over antiparallel dimeric and monomeric quadruplexes than compounds 2-3 with the longer polyether *** intriguingly,four molecules of compound 1 might externally stack on the end G-quartets of the two mixed-type G-quadruplex units on one dimeric *** addition,these diberberine derivatives displayed good inhibitory activities toward four tumor cell lines.

关键词： Di-berberine dimeric quadruplex DNA telomere binding selectivity

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Effect of Whole Body Vibration Therapy on Circulating Seroto...