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Long-term in vivo chimeric cells tracking in non-human primate

Protein & Cell 2024年第3期15卷 207-222页

作者： Junmo Wu Yu Kang Xiang Luo Shaoxing Dai Yuxi Shi zhuoyao li Zengli Tang Zhenzhen Chen Ran Zhu Pengpeng Yang Zifan li Hong Wang Xinglong Chen Ziyi Zhao Weizhi ji Yuyu Niu State Key Laboratory of Primate Biomedical Research Institute of Primate Translational MedicineKunming University of Science and TechnologyKunming 650500China Yunnan Key Laboratory of Primate Biomedical Research Kunming 650500China Faculty of life Science and Technology Kunming University of Science and TechnologyKunming 650500China

Non-human primates(NHPs)are increasingly used in preclinical trials to test the safety and efficacy of biotech-nology therapies.Nonetheless,given the ethical issues and costs associated with this model,it would be highly advantageous to use NHP cellular models in clinical studies.However,developing and maintaining the naive state of primate pluripotent stem cells(PSCs)remains difficult as does in vivo detection of PSCs,thus limiting biotech-nology application in the cynomolgus monkey.Here,we report a chemically defined,xeno-free culture system for culturing and deriving monkey PSCs in vitro.The cells display global gene expression and genome-wide hypometh-ylation patterns distinct from monkey-primed cells.We also found expression of signaling pathways components that may increase the potential for chimera formation.Crucially for biomedical applications,we were also able to integrate bioluminescent reporter genes into monkey PsCs and track them in chimeric embryos in vivo and in vitro.The engineered cells retained embryonic and extra-embryonic developmental potential.Meanwhile,we generated a chimeric monkey carrying bioluminescent cells,which were able to track chimeric cells for more than 2 years in living animals.Our study could have broad utility in primate stem cell engineering and in utilizing chimeric monkey models forclinical studies.

关键词： pluripotent stem cells bioluminescence imaging non-human primates

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Current state of stem cell research in non-human primates:an overview

Medical Review

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Medical Review 2023年第4期3卷 277-304页

作者： Junmo Wu Yuxi Shi Shanshan Yang Zengli Tang Zifan li zhuoyao li Jiawei Zuo Weizhi Ji Yuyu Niu Kunming University of Science and Technology KunmingYunnan ProvinceChina Yunnan Key Laboratory of Primate Biomedical Research KunmingYunnan ProvinceChina

The remarkable similarity between non-human primates(NHPs)and humans establishes them as essential models for understanding human biology and diseases,as well as for developing novel therapeutic strategies,thereby providing more comprehensive reference data for clinical treatment.Pluripotent stem cells such as embryonic stem cells and induced pluripotent stem cells provide unprecedented opportunities for cell therapies against intractable diseases and injuries.As continue to harness the potential of these biotechnological therapies,NHPs are increasingly being employed in preclinical trials,serving as a pivotal tool to evaluate the safety and efficacy of these interventions.Here,we review the recent advancements in the fundamental research of stem cells and the progress made in studies involving NHPs.

关键词： non-human primates cell therapy stem cell

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Direct observation of the plasmon-enhanced palladium catalysis with single-molecule fluorescence microscopy

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Nano Research 2023年第7期16卷 8817-8826页

作者： zhuoyao li Rajkumar Devasenathipathy Junjie Wang liuyingzi Yu Yan liang Huixiang Sheng Yameng Zhu Hai li Hiroshi Uji-i Xiao Huang Gang Lu School of Flexible Electronics(Future Technologies) Key Laboratory of Flexible Electronicsand Institute of Advanced MaterialsNanjing Tech University(NanjingTech)Nanjing 211816China Department of Chemistry KU LeuvenCelestijnenlaan 200FLeuven 3001Belgium Research Institute for Electronic Science Hokkaido UniversityN20W10Kita wardSapporoHokkaido 001-0020Japan National Laboratory of Solid State Microstructures Nanjing UniversityNanjing 210093China

Plasmonic nanostructures have been proved effective not only in catalyzing chemical reactions,but also in improving the activity of non-plasmonic photocatalysts.It is essential to reveal the synergy between the plasmonic structure and the non-plasmonic metal photocatalyst for expounding the underlying mechanism of plasmon-enhanced catalysis.Herein,the enhancement of resazurin reduction at the heterostructure of silver nanowire(AgNW)and palladium nanoparticles(PdNPs)is observed in situ by single-molecule fluorescence microscopy.The catalysis mapping results around single AgNW suggest that the catalytic activity of PdNPs is enhanced for~20 times due to the excitation of localized surface plasmon resonance(LSPR)in the vicinity of the AgNW.This catalysis enhancement is also highly related to the wavelength and polarization of the excitation light.In addition,the palladium catalysis is further enhanced by~10 times in the vicinity of a roughened AgNW or a AgNW-AgNW nanogap because of the improvement of catalytic hotspots.These findings clarify the contribution of plasmon excitation in palladium catalysis at microscopic scale,which will help to deepen the understanding of the plasmon-enhanced photocatalysis and provide a guideline for developing highly efficient plasmon-based photocatalysts.

关键词： surface plasmon palladium nanoparticles photocatalysis enhancement hot carrier super-resolution fluorescence microscopy

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Plasmon-generated hot holes for chemical reactions

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Nano Research 2020年第12期13卷 3183-3197页

作者： Chengyu Zhang Fucan Jia zhuoyao li Xiao Huang Gang Lu Key Laboratory of Flexible Electronics(KLOFE)and Institute of Advanced Materials(IAM) Nanjing Tech University(NanjingTech)30 South Puzhu RoadNanjing211816China

Plasmonic nanostructures have been widely used for photochemical conversions due to their unique and easy-tuning optical properties in visible and near-infrared range.Compared with the plasmon-generated hot electrons,the hot holes usually have a shorter lifetime,which makes them more difficult to drive redox reactions.This review focuses on the photochemistry driven by the plasmon-generated hot holes.First,we discuss the generation and energy distribution of the plasmon-generated hot carriers,especially hot holes.Then,the dynamics of the hot holes are discussed at the interface between plasmonic metal and semiconductor or adsorbed molecules.Afterwards,the utilization of these hot holes in redox reactions is reviewed on the plasmon-semiconductor heterostructures as well as on the surface of the molecule-adsorbed plasmonic metals.Finally,the remaining challenges and future perspectives in this field are presented.This review will be helpful for further improving the efficiency of the photochemical reactions involving the plasmon-generated hot holes and expanding the applications of these hot holes in varieties of chemical reactions,especially the ones with high conversion rate and selectivity.

关键词： surface plasmon hot hole photochemistry photocatalysis

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基于特征解耦表征学习的无监督混合失真图像复原方法

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计算机辅助设计与图形学学报 2024年第2期36卷 236-247页

作者：符颖李卓遥朱欣宇龚敏学周激流成都信息工程大学计算机学院成都610225 四川省2011图形图像与空间信息协同创新中心成都610225

针对真实场景下多种混合失真组合的多任务图像复原,结合生成对抗网络与编码器提出了一种基于无监督对偶学习的图像复原方法.该方法引入特征解耦模块,通过修正基于增益控制的归一化,将不同退化机制的特征表示分配到不同的特征通道中,使... 详细信息

针对真实场景下多种混合失真组合的多任务图像复原,结合生成对抗网络与编码器提出了一种基于无监督对偶学习的图像复原方法.该方法引入特征解耦模块,通过修正基于增益控制的归一化,将不同退化机制的特征表示分配到不同的特征通道中,使得不同退化特征表达相互独立,实现了通道上的特征解耦.同时,为了进一步过滤掉退化机制的特征表示并保持原图像内容信息的细节,利用通道注意力机制实现特征解耦后自适应地选择有用的特征表示,使其适用于真实场景下混合失真组合的图像复原任务.实验结果表明,所提算法在单一退化类型GoPro数据集上对比基于尺度循环网络的算法(scale-recurrent network,SRN),峰值信噪比和结构相似性2项指标值分别提高了0.499 dB和0.044;在混合退化类型DIV2K数据集上对比基于操作选择注意力网络的算法(operation-wise attention network,OWAN),峰值信噪比和结构相似性2项指标值分别提高了0.163 dB和0.015,在实现复原图像的同时,保留了图像的纹理和细节信息.

关键词：混合失真图像图像复原特征解耦注意力机制

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国内学界乡村休闲研究综述及展望——基于CiteSpace知识图谱的可视化分析

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成都大学学报（社会科学版） 2024年第2期 28-40页

作者：赵春艳李卓遥夏银环苏雯贵州财经大学工商管理学院贵州贵阳550000

新时代背景下,乡村休闲是实现乡村全面振兴并提升村民美好生活质量的重要方面。本文运用CiteSpace软件对中国知网(CNKI)文献总库1994—2023年刊发的176篇文献进行共现分析及聚类分析,研究发现:(1)研究阶段分为缓慢萌芽期(1994—2004年)... 详细信息

新时代背景下,乡村休闲是实现乡村全面振兴并提升村民美好生活质量的重要方面。本文运用CiteSpace软件对中国知网(CNKI)文献总库1994—2023年刊发的176篇文献进行共现分析及聚类分析,研究发现:(1)研究阶段分为缓慢萌芽期(1994—2004年)、波动增长期(2005—2023年);(2)研究主体多分散在综合性高等院校不同院系,缺乏专门的研究机构对乡村休闲的系统性关注;(3)研究内容主要集中在对乡村休闲价值、乡村休闲生活变迁、城乡休闲行为比较、乡村特殊人群休闲活动、乡村休闲文化建设五个方面;(4)研究现状呈现出内容丰富、视角多元、方法多样的同时,也存在系统性不足、理论性不够、融合性欠缺的问题。因此未来可以组建专门的学术研究机构,加强乡村休闲的理论探索,尝试研究方法的融合运用,以提升国内学界对乡村休闲的研究。

关键词：乡村休闲研究综述 CiteSpace 可视化分析

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Next‑Generation Sequencing‑Based Copy Number Variation Analysis in Chinese Patients with Primary Ciliary Dyskinesia Revealed Novel DNAH5 Copy Number Variations

Phenomics

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Phenomics 2024年第1期4卷 24-33页

作者： Weicheng Chen zhuoyao Guo Mengru li Wei Sheng Guoying Huang Cardiovascular Center Children’s Hospital of Fudan University399 Wanyuan RoadShanghai 201102China Respirology Department Children’s Hospital of Fudan UniversityShanghai 201102China

Primary ciliary dyskinesia(PCD)is a rare disorder characterized by extensive genetic heterogeneity.However,in the genetic pathogenesis of PCD,copy number variation(CNV)has not received sufcient attention and has rarely been reported,especially in China.Next-generation sequencing(NGS)followed by targeted CNV analysis was used in patients highly suspected to have PCD with negative results in routine whole-exome sequencing(WES)analysis.Quantitative real-time polymerase chain reaction(qPCR)and Sanger sequencing were used to confrm these CNVs.To further characterize the ciliary phenotypes,high-speed video microscopy analysis(HSVA),transmission electron microscopy(TEM),and immunofuorescence(IF)analysis were used.Patient 1(F1:II-1),a 0.6-year-old girl,came from a nonconsanguineous family-I.She presented with situs inversus totalis,neonatal respiratory distress,and sinusitis.The nasal nitric oxide level was markedly reduced.The respiratory cilia beat with reduced amplitude.TEM revealed shortened outer dynein arms(ODA)of cilia.chr5:13717907-13722661del spanning exons 71–72 was identifed by NGS-based CNV analysis.Patient 2(F2:IV-4),a 37-year-old man,and his eldest brother Patient 3(F2:IV-2)came from a consanguineous family-II.Both had sinusitis,bronchiectasis and situs inversus totalis.The respiratory cilia of Patient 2 and Patient 3 were found to be uniformly immotile,with ODA defects.Two novel homozygous deletions chr5:13720087_13733030delinsGTTTTC and chr5:13649539_13707643del,spanning exons 69–71 and exons 77–79 were identifed by NGS-based CNV analysis.Abnormalities in DNA copy number were confrmed by qPCR amplifcation.IF showed that the respiratory cilia of Patient 1 and Patient 2 were defcient in dynein axonemal heavy chain 5(DNAH5)protein expression.This report identifed three novel DNAH5 disease-associated variants by WES-based CNV analysis.Our study expands the genetic spectrum of PCD with DNAH5 in the Chinese population.

关键词： Primary ciliary dyskinesia DNAH5 gene Copy number variation Phenotypic heterogeneity

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Designing metal sulfide-based cathodes and separators for suppressing polysulfide shuttling in lithium-sulfur batteries

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Nano Research 2024年第4期17卷 2574-2591页

作者： Guoyin Zhu Qingzhu Wu Xianghua Zhang Yuwen Bao Xuan Zhang zhuoyao Shi Yizhou Zhang lianbo Ma Institute of Advanced Materials and Flexible Electronics(IAMFE) School of Chemistry and Materials ScienceNanjing University of Information Science&TechnologyNanjing 210044China School of Materials Science and Engineering Anhui University of TechnologyMaanshan 243002China School of Mechanical Engineering Jiangsu University of TechnologyChangzhou 213001China

lithium-sulfur(li-S)batteries,known for their high energy density,are attracting extensive research interest as a promising next-generation energy storage technology.However,their widespread use has been hampered by certain issues,including the dissolution and migration of polysulfides,along with sluggish redox kinetics.Metal sulfides present a promising solution to these obstacles regarding their high electrical conductivity,strong chemical adsorption with polysulfides,and remarkable electrocatalytic capabilities for polysulfide conversion.In this review,the recent progress on the utilization of metal sulfide for suppressing polysulfide shuttling in li-S batteries is systematically summarized,with a special focus on sulfur hosts and functional separators.The critical roles of metal sulfides in realizing high-performing li-S batteries have been comprehensively discussed by correlating the materials’structure and electrochemical performances.Moreover,the remaining issues/challenges and future perspectives are highlighted.By offering a detailed understanding of the crucial roles of metal sulfides,this review dedicates to contributing valuable knowledge for the pursuit of high-efficiency li-S batteries based on metal sulfides.

关键词： lithium-sulfur batteries metal sulfides polysulfide shuttling sulfur hosts separator coating layers

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汉江上游不同林龄麻栎林枯落物的水文功能评价

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生态环境学报 2022年第1期31卷 44-51页

作者：张淑兰韩勇杨盼闫育盈刘昭雪李卓瑶安康学院旅游与资源环境学院陕西安康725000 安康学院陕西省科协院士专家工作站陕西安康725000

麻栎林普遍存在于汉江上游,其涵养水源能力的发挥对维持汉江上游流域生态平衡具有重要的作用。于2020年12月对汉江上游天柱山3个不同林龄(15 a、25 a、33 a)的麻栎林样地进行了枯落物厚度和蓄积量调查,利用浸泡法测定了枯落物各水文功... 详细信息

麻栎林普遍存在于汉江上游,其涵养水源能力的发挥对维持汉江上游流域生态平衡具有重要的作用。于2020年12月对汉江上游天柱山3个不同林龄(15 a、25 a、33 a)的麻栎林样地进行了枯落物厚度和蓄积量调查,利用浸泡法测定了枯落物各水文功能指标,运用熵权法对3个林龄麻栎林枯落物的水源涵养能力进行了综合评价。结果表明:麻栎林枯落物总厚度在3.0—4.8 cm,蓄积量介于13.76—14.39 t·hm^(−2),二者在林龄之间差异不显著(P=0.591;P=0.993);不同林龄麻栎林内半分解层枯落物的瞬时持水量和吸水速率略大于未分解层的;二者的枯落物瞬时持水量均在10 min内快速增加,20 min后缓慢增加,其与浸泡时间呈对数关系,R^(2)为0.7646—0.9606;未分解层枯落物的瞬时持水量表现为15 a>33 a>25 a,而半分解层的则表现6 h之前25 a的略大于15 a的,之后二者的持水量几乎相等,但二者均大于33 a的;相应的不同林龄内未分解层和半分解的枯落物吸水速率均在20 min内很快降低,2 h之后吸水速率以接近0的趋势变小,其与浸泡时间呈幂函数关系,R2为0.9933—0.9943;未分解层的枯落物瞬时吸水速率表现为15 a>33 a>25 a,而半分解层的表现为25 a>15 a>33 a;不同林龄的枯落物自然持水率差异显著(P=0.017),总枯落物层、未分解层和半分解层的自然含水率的大小依次均为15 a>33 a>25 a,而自然持水量、最大持水量和最大持水率在林龄之间差异均不显著(P=0.508;P=0.428;P=0.997);不同林龄内枯落物最大拦蓄量介于21.22—26.03 t·hm^(−2),有效拦蓄量介于6.45—14.52 t·hm^(−2),二者在不同林龄之间差异不显著(P=0.331),主要受枯落物自然持水率影响;水源涵养能力综合评价大小依次为33 a(35.45)>25 a(34.10)>15 a(30.35)。

关键词：枯落物蓄积量水文功能林龄麻栎林汉江上游

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日本骨科协会腰痛评估量表的汉化与应用

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中国中医基础医学杂志 2023年第2期29卷 269-271页

作者：李桌瑶田子睿姚敏杨龙李振军王拥军崔学军张宇上海中医药大学附属龙华医院上海200032 教育部筋骨理论与治疗重点实验室上海200032 上海中医药大学上海201203 上海市第八人民医院上海200235 甘肃省中医院兰州730050 辽宁中医大学沈阳110847

日本骨科协会腰痛评估量表(Japanese Orthopaedic Association Back Pain Evaluation Questionnaire,JOABPEQ)是日本骨科协会临床评价委员会2007年研制的应用于检测腰痛程度的量表。目前该量表已翻译成多种语言,在国际上得到广泛使用。... 详细信息

日本骨科协会腰痛评估量表(Japanese Orthopaedic Association Back Pain Evaluation Questionnaire,JOABPEQ)是日本骨科协会临床评价委员会2007年研制的应用于检测腰痛程度的量表。目前该量表已翻译成多种语言,在国际上得到广泛使用。由于缺少简体中文版本,JOABPEQ尚未在中国广泛使用。本课题组采用翻译-合并-反译-专家委员会-预检验的方式对该量表进行了汉化,并纳入184名以汉语为母语的腰痛患者进行检测。经检测认为汉化版JOABPEQ具有良好的信度和效度。本文对汉化版JOABPEQ进行介绍并说明其使用方法,以期该量表在中国的相关研究中得到推广和应用。

关键词：日本骨科协会腰痛评估量表腰痛汉化信度

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