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Relationship between IFN-γ gene polymorphism and susceptibility to intrauterine HBV infection

World Journal of Gastroenterology 2006年第18期12卷 2928-2931页

作者： Hui Yu Qi-Rong zhu shao-qing Gu Lin-E Fei Department of Infectious Disease Children's Hospital of Fudan University Shanghai 200032 China

AIM： To explore the susceptibility of children to intrauterine HBV infection by studying the relationship between IFN-γ gene polymorphism, including IFN-γ＋874A/T single nucleotide polymorphism（SNP） and CA repeat microsatellite polymorphism and intrauterine HBV infection. METHODS： A TaqMan fluorescence polymerase chain reaction in the IFN-γ＋874A/T single nucleotide polymorphism was tested in the intrauterine HBV infection group（group Ⅰ） and the normal immune children group（group Ⅱ）. Capillary electrophoresis was performed in the above two groups to assay the IFN-γ, CA repeat microsatellite polymorphism. RESULTS： Frequencies of AA, AT and TT genotypes were 67.4%, 19.6% and 13.0% in the intrauterine HBV infection group, and 45.2%, 30.1% and 24.7% in the normal immune children group, respectively. A significant difference was found in the frequency distribution of IFN-γ＋874 genotype between the two groups （x^2 = 5.102, P = 0.02389）. In the intrauterine HBV infection group the AA genotype was more common than in the normal immune group. Frequency of IFN-γ＋874A allele was 77.17% in the intrauterine HBV infection group, and 60.27% in the normal immune children group. In the intrauterine HBV infection group the IFN-γ＋874A allele was more common than in normal immune group. A significant difference was found in the frequency distribution between the two groups （x^2= 7.238, P= 0.02389, OR = 2.228, 95% CI = 1.244-3.992）. （CA12）^＋/（CA12）^＋ of IFN-γ CA microsatellite polymorphism was 11.90% in the intrauterine HBV infection group and 26.47% in the normal immune children group. A significant difference was found in the frequency distribution between the two groups （x^2 = 5.64, P = 0.0176）. Frequency of IFN-γ CA repeat was 25% in the intrauterine HBV infection group and 43.38% in the normal immune children group. The frequency of IFN-γ CA repeat was less in the intrauterine HBV infection group than in normal immune group. A significant difference was found in the frequency dis

关键词： Interferon-γ Gene polymorphism Hepatitis B virus Intrauterine

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Uncovering the dynamic evolution of nucleotidebinding site-leucine-rich repeat(NBS-LRR) genes in Brassicaceae

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Journal of Integrative Plant Biology 2016年第2期58卷 165-177页

作者： Yan-Mei Zhang zhu-qing shao Qiang Wang Yue-Yu Hang Jia-Yu Xue Bin Wang Jian-Qun Chen State Key Laboratory of Pharmaceutical Biotechnology Center of Plant Genetics and Molecular EvolutionSchool of Life SciencesNanjing University Institute of Botany Jiangsu Province and Chinese Academy of Sciences

Plant genomes harbor dozens to hundreds of nucleotide-binding site-leucine-rich repeat （NBS-LRR） genes; however, the long-term evolutionary history of these resistance genes has not been fully understood, This study focuses on five Brassicaceae genomes and the Carica papaya genome to explore changes in NBS-LRR genes that have taken place in this Rosid II lineage during the past 72 million years. Various numbers of NBS-LRR genes were identified from Arabidopsis lyrata （198）, A. thaliana （165）, Brassica rapa （204）, Capsella rubella （127）, Thellungiella salsuginea （88）, and C. papaya （51）. In each genome, the identified NBS-LRR genes were found to be unevenly distributed among chromosomes and most of them were clustered together. Phylogenetic analysis revealed that, before and after Brassicaceae speciation events, both toll/interleukin-1 receptor-NBS-LRR （TNL） genes and non-toll/interleukin-1 receptor-NBS-LRR （nTNL） genes exhibited a pattern of first expansion and then contraction, suggesting that both subclasses of NBS-LRR genes were responding to pathogen pressures synchronically. Further, by examining the gain/loss of TNL and nTNL genes at different evolutionary nodes, this study revealed that both events often occurred more drastically in TNL genes. Finally, the phylogeny of nTNL genes suggested that this NBS-LRR subclass is composed of two separate ancient gene types： RPW8-NBS-LRR and Coiled-coiI-N BS-LRR.

关键词： Dynamic evolution nucleotide-binding site-leucine-rich repeat gene phylogenetic relationship plant disease resistance

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The RNAome landscape of tomato during arbuscular mycorrhizal symbiosis reveals an evolving RNA layer symbiotic regulatory network

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Plant Communications 2023年第1期4卷 148-166页

作者： Zhen Zeng Yang Liu Xing-Yu Feng Sai-Xi Li Xing-Mei Jiang Jian-Qun Chen zhu-qing shao State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjing 210023China

Arbuscular mycorrhizal symbiosis(AMS)is an ancient plant-fungus relationship that is widely distributed in terrestrial *** formation of symbiotic structures and bidirectional nutrient exchange requires the regulation of numerous ***,the landscape of RNAome during plant AMS involving different types of regulatory RNA is poorly *** this study,a combinatorial strategy utilizing multiple sequencing approaches was used to decipher the landscape of RNAome in tomato,an emerging AMS *** annotation of the tomato genome was improved by a multiple-platform sequencing strategy.A total of 3,174 protein-coding genes were upregulated during AMS,42%of which were alternatively ***-transcriptome analysis revealed that genes from 24 orthogroups were consistently induced by AMS in eight phylogenetically distant *** additional orthogroups were specifically induced by AMS in all surveyed dicot AMS host ***,these orthogroups were absent or not induced in monocots and/or non-AMS hosts,suggesting a continuously evolving AMS-responsive network in addition to a conserved core regulatory ***,we detected 587 lncRNAs,ten miRNAs,and 146 circRNAs that responded to AMS,which were incorporated to establish a tomato AMSresponsive,competing RNA-responsive endogenous RNA(ceRNA)***,a tomato symbiotic transcriptome database(TSTD,https://***/TSTD)was constructed to serve as a resource for deep deciphering of the AMS regulatory *** results help elucidate the reconfiguration of the tomato RNAome during AMS and suggest a sophisticated and evolving RNA layer responsive network during AMS processes.

关键词： tomato RNAome landscape arbuscular mycorrhizal symbiosis evolution ceRNA network

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An angiosperm NLR Atlas reveals that NLR gene reduction is associated with ecological specialization and signal transduction component deletion

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Molecular Plant 2021年第12期14卷 2015-2031页

作者： Yang Liu Zhen Zeng Yan-Mei Zhang Qian Li Xing-Mei Jiang Zhen Jiang Ji-Hong Tang Dijun Chen Qiang Wang Jian-Qun Chen zhu-qing shao State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjing 210023China Jiangsu Key Laboratory for the Research and Utilization of Plant Resources Institute of BotanyJiangsu Province and Chinese Academy of SciencesNanjing 210014China

Nucleotide-binding leucine-rich-repeat(NLR)genes comprise the largest family of plant disease-resis-tance *** NLR genes are phylogenetically divided into the TNL,CNL,and RNL *** copy numbers and subclass composition vary tremendously across angiosperm ***,the evolutionary associations between genomic NLR content and ecological adaptation,or between NLR content and signal transduction components,are poorly characterized because of limited genome *** this study,we established an angiosperm NLR atlas(ANNA,https://***/ANNA/)that includes NLR genes from over 300 angiosperm *** ANNA,we revealed that NLR copy numbers differ up to 66-fold among closely related species owing to rapid gene loss and ***,NLR contraction was associated with adaptations to aquatic,parasitic,and carnivorous *** convergent NLR reduction in aquatic plants resembles the lack of NLR expansion during the long-term evolution of green algae before the colonization of land.A co-evolutionary pattern between NLR subclasses and plant immune pathway components was also identified,suggesting that immune pathway deficiencies may drive TNL ***,we identified a conserved TNL lineage that may function independently of the EDS1-SAG101-NRG1 ***,these findings provide new insights into the evolution of NLR genes in the context of ecological adaptation and genome content variation.

关键词： NLR genes plant disease resistance EDS1 gene family gene family evolution angiosperms

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Comprehensive regulatory networks for tomato organ development based on the genome and RNAome of MicroTom tomato

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Horticulture Research 2023年第9期10卷 13-23页

作者： Jia-Yu Xue Hai-Yun Fan Zhen Zeng Yu-Han Zhou Shuai-Ya Hu Sai-Xi Li Ying-Juan Cheng Xiang-Ru Meng Fei Chen zhu-qing shao Yves Van de Peer College of Horticulture Academy for Advanced Interdisciplinary StudiesNanjing Agricultural UniversityNanjing 210095China State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjing 210023China College of Tropical Crops Sanya Nanfan Research InstituteHainan UniversityHaikou 570228China Hainan Yazhou Bay Seed Laboratory Sanya 572025China Department of Plant Biotechnology and Bioinformatics VIB-UGent Center for Plant Systems BiologyGhent UniversityB-9052 GhentBelgium Department of Biochemistry Genetics and MicrobiologyUniversity of PretoriaPretoria 0028South Africa

MicroTom has a short growth cycle and high transformation efficiency,and is a prospective model plant for studying organ development,metabolism,and plant–microbe ***,with a newly assembled reference genome for this tomato cultivar and abundant RNA-seq data derived from tissues of different organs/developmental stages/treatments,we constructed multiple gene co-expression networks,which will provide valuable clues for the identification of important genes involved in diverse regulatory pathways during plant growth,*** mycorrhizal symbiosis and fruit ***,non-coding RNAs,including miRNAs,lncRNAs,and circRNAs were also identified,together with their potential *** networks between different types of non-coding RNAs(miRNA-lncRNA),and non-coding RNAs and genes(miRNA-mRNA and lncRNA-mRNA)were constructed as *** results and data will provide valuable information for the study of organ differentiation and development of this important ***,we established a database(http://***/microTomBase/)with genomic and transcriptomic data,as well as details of gene co-expression and interacting networks on MicroTom,and this database should be of great value to those who want to adopt MicroTom as a model plant for research.

关键词： metabolism organs networks

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A chromosome-level genome assembly of rugged rose (Rosa rugosa) provides insights into its evolution, ecology, and floral characteristics

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Horticulture Research 2021年第1期8卷 1939-1951页

作者： Fei Chen Liyao Su Shuaiya Hu Jia-Yu Xue Hui Liu Guanhua Liu Yifan Jiang Jianke Du Yushan Qiao Yannan Fan Huan Liu Qi Yang Wenjie Lu zhu-qing shao Jian Zhang Liangsheng Zhang Feng Chen Zong-Ming(Max)Cheng College of Horticulture Nanjing Agricultural UniversityNanjing 210095China Academy for Advanced Interdisciplinary Studies Nanjing Agricultural UniversityNanjing 210095China BGI-Shenzhen Beishan Industrial ZoneYantian DistrictShenzhen 518083China Department of Biology University of CopenhagenCopenhagenDenmark Grandomics Biosciences Co. LtdWuhanChina School of Life Sciences Nanjing UniversityNanjingChina College of life science Nantong UniversityNantongChina Genomics and Genetic Engineering Laboratory of Ornamental Plants College of Agriculture and BiotechnologyZhejiang UniversityHangzhouChina Department of plant sciences University of TennesseeKnoxvilleTNUSA

Rosa rugosa,commonly known as rugged rose,is a perennial ornamental *** produces beautiful flowers with a mild fragrance and colorful seed *** many other cultivated roses,*** adapts to a wide range of habitat types and harsh environmental conditions such as salinity,alkaline,shade,drought,high humidity,and frigid ***,we produced and analyzed a high-quality genome sequence for *** to understand its ecology,floral characteristics and *** HiFi reads were initially used to construct the draft genome of ***,and then Hi-C sequencing was applied to assemble the contigs into 7 *** obtained a 382.6Mb genome encoding 39,704 protein-coding *** genome of *** appears to be conserved with no additional whole-genome duplication after the gamma whole-genome triplication(WGT),which occurred~100 million years ago in the ancestor of core *** on a comparative analysis of the high-quality genome assembly of *** and other high-quality Rosaceae genomes,we found a unique large inverted segment in the Chinese rose *** and a retroposition in strawberry caused by post-WGT *** also found that floral development-and stress response signaling-related gene modules were retained after the *** MADS-box genes involved in floral development and the stress-related transcription factors DREB2A-INTERACTING PROTEIN 2(DRIP2)and PEPTIDE TRANSPORTER 3(PTR3)were found to be positively selected in evolution,which may have contributed to the unique ability of this plant to adapt to harsh *** summary,the high-quality genome sequence of *** provides a map for genetic studies and molecular breeding of this plant and enables comparative genomic studies of Rosa in the near future.

关键词： breeding characteristics evolution

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基于一测多评法的大枣药材三萜酸类化学成分检测分析方法的建立

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中草药 2016年第21期47卷 3884-3889页

作者：郭盛段金廒朱邵晴钱大玮南京中医药大学江苏省中药资源产业化过程协同创新中心/中药资源产业化与方剂创新药物国家地方联合工程研究中心江苏南京210023

目的建立基于一测多评法(QAMS)同时测定大枣药材中多种三萜酸类成分的分析方法,为大枣药材的品质评价和质量控制提供技术支撑。方法采用HPLC-ELSD技术,以白桦脂酸对照品为内参物,测定其与齐墩果酸、熊果酸、桦木酮酸、齐墩果酮酸、熊果... 详细信息

目的建立基于一测多评法(QAMS)同时测定大枣药材中多种三萜酸类成分的分析方法,为大枣药材的品质评价和质量控制提供技术支撑。方法采用HPLC-ELSD技术,以白桦脂酸对照品为内参物,测定其与齐墩果酸、熊果酸、桦木酮酸、齐墩果酮酸、熊果酮酸的相对校正因子,采用相对校正因子计算此5种三萜酸类化学成分的量,实现一测多评;同时比较外标法所测得的大枣药材中该6种化学成分的实际值与经QAMS所得的计算值之间的差异,以验证QAMS的适用性、可行性和重复性。结果相对校正因子的重现性较好,15批大枣药材样品中6种三萜酸类化学成分的QAMS计算值与外标法实测值之间无显著性差异。结论以白桦脂酸为内参物建立的同时测定齐墩果酸、熊果酸、桦木酮酸、齐墩果酮酸、熊果酮酸的一测多评方法操作简便,测定结果准确,实现了采用1种对照品同步测定大枣样品中6种化学成分的量,可为大枣药材质量标准的制定和提升提供支撑,为中药多指标成分质量评价模式的建立提供借鉴。

关键词：大枣一测多评三萜酸分析方法标准制定

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Effect of pyrite content on chalcopyrite flotation under different regrinding conditions

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 49-57页

作者： Zejun Wang qing Shi Guofan Zhang Yuxuan zhu Binbin Li School of Minerals Processing and Bioengineering Central South UniversityChangsha 410083China Key Laboratory of Hunan Province for Clean and Efficient Utilization of Strategic Calcium-containing Mineral Resources Central South UniversityChangsha 410083China

This study aimed to investigate the effect of varying pyrite(Py)content on copper(Cu)in the presence of different regrinding conditions,which were altered using different types of grinding media:iron,ceramic balls,and their mixture,followed by flotation in the cleaner *** flotation performance of rough Cu concentrate can be improved by changing the regrinding conditions based on the Py *** electron microscopy,X-ray spectrometry,ethylenediaminetetraacetic acid disodium salt extraction,and X-ray photoelectron spectroscopy studies illustrated that when the Py content was high,the use of iron media in regrinding promoted the generation of hydrophilic Fe OOH on the surface of Py and improved the Cu *** ceramic medium with a low Py content prevented excessive Fe OOH from covering the surface of chalcopyrite(Cpy).Electrochemical studies further showed that the galvanic corrosion current of Cpy-Py increased with the addition of Py and became stronger with the participation of iron media.

关键词： chalcopyrite regrinding media pyrite galvanic corrosion flotation

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黄芪花营养成分分析与资源价值评价

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中草药 2018年第18期49卷 4408-4416页

作者：李园常相伟朱邵晴朱振华钱大玮朱悦王汉卿郭盛段金廒南京中医药大学江苏省中药资源产业化过程协同创新中心国家中医药管理局中药资源循环利用重点研究室中药资源产业化与方剂创新药物国家地方联合工程研究中心江苏南京210023 宁夏医科大学药学院宁夏银川750021

目的明晰黄芪花中资源性化学成分组成,探讨其资源潜在利用价值。方法采用分光光度法对黄芪花中总多糖、水溶性蛋白进行分析评价;采用HPLC-PDA/ELSD法对黄芪花中单糖寡糖组成及含量进行分析;采用GC-MS法对黄芪花中挥发性成分及脂肪酸类... 详细信息

目的明晰黄芪花中资源性化学成分组成,探讨其资源潜在利用价值。方法采用分光光度法对黄芪花中总多糖、水溶性蛋白进行分析评价;采用HPLC-PDA/ELSD法对黄芪花中单糖寡糖组成及含量进行分析;采用GC-MS法对黄芪花中挥发性成分及脂肪酸类成分和含量进行分析;采用UPLC-TQ-MS法对黄芪花中核苷类及氨基酸类组成及含量进行分析。结果黄芪花中含有多糖类(47.02 mg/g)、水溶性蛋白质(470.66 mg/g)、果糖(45.46 mg/g)、葡萄糖(8.71 mg/g)、蔗糖(1.05 mg/g)营养物质;检测出黄芪花中32种挥发性成分,其中以含氧衍生物为其主要组成成分;6种核苷类和15种游离氨基酸类资源性化学成分,总量分别达2.77和6.52 mg/g。在黄芪花中还检出8种脂肪酸类成分,其中肉豆蔻酸、棕榈酸和油酸为其主要组成成分。结论阐明了黄芪花中各类型营养成分的组成及含量,为黄芪花的系统利用与精细化开发提供了科学依据。

关键词：黄芪花营养类成分资源化价值 HPLC-PDA/ELSD 多糖水溶性蛋白质果糖葡萄糖蔗糖

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外墙外保温在电力建筑中的应用

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山西建筑 2009年第2期35卷 245-246页

作者：朱少青大同新电电力有限责任公司

以聚苯板为例,结合电力建筑特点,介绍了外墙外保温施工技术,从材料选用、施工操作要点等方面进行了详细阐述,并指出外墙外保温在电力建筑中有良好的推广前景。

关键词：聚苯板电力建筑外保温施工技术

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