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Functional protection of pentoxifylline against spinal cord ischemia/reperfusion injury in rabbits： necrosis and apoptosis effects

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Chinese Medical Journal 2008年第23期121卷 2444-2449页

作者： zhu dan-jie XIA Bing BI Qing ZHANG Shui-jun QIU Bin-song ZHAO Chen Department of Orthopedics Zhejiang Provincial People's Hospital Hangzhou Zhejiang 310014 China

Background Little is known about neuronal death mechanisms following spinal cord ischemia. The present study aimed to investigate the protective effect of pentoxifylline （PTX） against spinal cord ischemia/reperfusion （I/R） injury. Methods Rabbits sustained spinal cord ischemia following 45 minutes cross-clamping of the infrarenal aorta. Experimental groups were as follows： the first group of animals （sham, n=-8） underwent laparotomy alone and served as the sham group; the second group （I/R, n=20） received carrier （3 ml saline solution） and served as the control group; the third group （PTX-A, n=20） received PTX intravenously 10 minutes prior to ischemia; and the fourth group （PTX-B, n=20） received PTX intravenously at the onset of reperfusion. Rabbits were evaluated for hind-limb motor function with the Tarlov scoring system at 48 hours. Serum was assayed with enzyme-linked immunosorbent assay for tumor necrosis factor α （TNF-α） and spinal cords were harvested for myeloperoxidase （MPO） activity, histopathological analysis, terminal deoxynucleotidyl transferase （TdT）-rnediated dUTP nick end labeling staining, platelet/endothelial ceU adhesion molecule-1 （PECAM-1） and caspase-3 immunohistochemistry, and the number of necrotic and apoptotic neuron were counted and data analyzed at 12, 24, 48 and 72 hours of reperfusion. Spinal cords were studied by electron microscopy. Results Improved Tarlov scores were seen in PTX-treated rabbits as compared with ischemic control rabbits at 48 hours. A significant reduction was found in TNF-α in serum, activity of MPO and immunoreactivity of the PECAM-1 and caspase-3 in PTX-treated rabbits. There were fewer apoptotic neurons than necrotic neurons （P 〈0.05）. A significant decrease in both necrotic and apoptotic neurons was observed in the PTX-treated groups （PTX-A and PTX-B） compared with the I/R group （P 〈0.05）. Both necrotic and apoptotic neurons were found with the electron microscope. Conclusions PTX may induce protection aga

关键词： spinal cord injury pentoxifylline necrosis apoptosis

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Increased excitatory amino acid transporter 2 levels in basolateral amygdala astrocytes mediate chronic stress–induced anxiety-like behavior

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Neural Regeneration Research 2025年第6期20卷 1721-1734页

作者： Xirong Xu Shoumin Xuan Shuai Chen dan Liu Qian Xiao jie Tu Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological Diseases Shenzhen-Hong Kong Institute of Brain ScienceShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina CAS Key Laboratory of Brain Connectome and Manipulation Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Brain Connectome and Behavior the Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina University of Chinese of Academy of Sciences BeijingChina Faculty of Life and Health Sciences Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina

The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with ***, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal *** knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was ob

关键词： anxiety astrocytes basolateral amygdala behavior dihydrokainic acid excitatory amino acid transporter 2 fiber photometry glutamate LDN-212320 transporter

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The emerging role of nitric oxide in the synaptic dysfunction of vascular dementia

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Neural Regeneration Research 2025年第2期20卷 402-415页

作者： Xiaorong Zhang Zhiying Chen Yinyi Xiong Qin Zhou Ling-Qiang zhu dan Liu Department of Pathology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Jiujiang Clinical Precision Medicine Research Center JiujiangJiangxi ProvinceChina Center for Cognitive Science and Transdisciplinary Studies Jiujiang UniversityJiangxi ProvinceChina Department of Neurology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Department of Rehabilitation Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Department of Pathophysiology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

With an increase in global aging,the number of people affected by cerebrovascular diseases is also increasing,and the incidence of vascular dementia-closely related to cerebrovascular risk-is increasing at an epidemic ***,few therapeutic options exist that can markedly improve the cognitive impairment and prognosis of vascular dementia *** in Alzheimer’s disease and other neurological disorders,synaptic dysfunction is recognized as the main reason for cognitive *** oxide is one of the ubiquitous gaseous cellular messengers involved in multiple physiological and pathological processes of the central nervous ***,nitric oxide has been implicated in regulating synaptic plasticity and plays an important role in the pathogenesis of vascular *** review introduces in detail the emerging role of nitric oxide in physiological and pathological states of vascular dementia and summarizes the diverse effects of nitric oxide on different aspects of synaptic dysfunction,neuroinflammation,oxidative stress,and blood-brain barrier dysfunction that underlie the progress of vascular ***,we propose that targeting the nitric oxide-sGC-cGMP pathway using certain specific approaches may provide a novel therapeutic strategy for vascular dementia.

关键词： endoplasmic reticulum stress endothelial nitric oxide synthase gene therapy nitric oxide NO-sGC-cGMP pathway synaptic dysfunction vascular dementia

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Effect of combination therapy with alginate dressing and mouse epidermal growth factor on epidermal stem cells in patients with refractory wounds

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Chinese Medical Journal 2012年第2期125卷 257-261页

作者： Bi Qing Zhang Qiong Ma Jun Xu Ming Zhang Shui-jun Qiu Bin-song Xia Bing Gu Hai-feng Hong Jian-fei Zhao Chen zhu dan-jie Zhejiang Prov Peoples Hosp Dept Orthoped Hangzhou 310014 Zhejiang Peoples R China Zhejiang Prov Peoples Hosp Dept Operating Room Hangzhou 310014 Zhejiang Peoples R China

Background The aim of this research was to determine the efficacy of combination therapy using an alginate dressing and mouse epidermal growth factor （mEGF） on proliferation and differentiation of epidermal stem cells （ESCs） in patients with refractory *** Eighteen patients （12 males and 6 females,aged from 18 to 61 years （mean 36.4 years）） with various skin wounds,were treated by dressing changing for one *** wounds were located in the foot （11）,calf （3）,thigh （2）and forearm （2）.The patients were randomly divided into 3 groups：alginate dressing and mEGF （group A; n=6）,mEGF （group B; n=6） and control （group C; n=6）.Wound closure indexes were measured at 7,14,21 and 28 *** were harvested for pathologic examination,at 7 and 14 days following *** 10 （CK10） and cytokeratin 15 （CK15） positive cells were evaluated using the super-sensitivity （SP） immunohistochemical staining *** Wound healing was promoted in groups A and *** group A,the wound closure index was increased significantly （P 〈0.05）,and in one case the maximum cure area reached 102 *** examination identified a thicker epidermis,active angiogenesis and enhanced granulation in group A compared with groups B and *** the SP immunohistochemical staining technique,we showed that ESCs in group A were bigger in size and larger in number than in groups B and ***,there was a significant difference in ESCs proliferation and differentiation between group A and group B （or C）.Conclusions Combination therapy using an alginate dressing and mEGF shows increased proliferation and differentiation of ESCs in patients with refractory wounds compared with those treated with mEGF alone.

关键词： alginates epidermal growth factor stem cells wound healing

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Cyano migration-mediated radical-polar crossover cyclopropanation

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Science China Chemistry 2025年第01期 241-248页

作者： Ziqiang Wang Yasu Chen jie Li Chen zhu Key Laboratory of Organic Synthesis of Jiangsu Province College of Chemistry Chemical Engineering and Materials ScienceSoochow University Frontiers Science Center for Transformative Molecules Zhangjiang Institute for Advanced Study and Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs Shanghai Jiao Tong University

We report a novel cyano migration-mediated radical-polar crossover process, whereby both the sulfonylcyanation of aliphatic alkenes and the formation of cyclopropanes are concurrently achieved. This transformation yields a diverse array of multisubstituted cyclopropanes under mild photocatalytic conditions. Mechanistic studies elucidate that the reaction proceeds via intramolecular nucleophilic substitution, leading to the formation of the cyclopropane derivatives.

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Hengqin:Gateway for Going Global

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China Today 2025年第1期74卷 50-53页

作者： zhu jie zhuhai Dahengqin Company Limited

The synergy and complementary strengths between Macao and Hengqin can create an important channel for Chinese companies to *** enterprises have placed much emphasis on going global in recent years,from traditional manufacturing giants to emerging tech unicorns,and from traditional sectors to emerging industries like electric vehicles,lithium batteries,and photovoltaic *** enthusiasm to internationalize reached unprecedented levels in 2024.

关键词： companies Gateway lithium

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固相萃取-气相色谱法测定水果、蔬菜中的氯丹

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化学试剂 2017年第3期39卷 263-265,316页

作者：郑俊旦朱杰丽浙江省林产品质量检测站国家林业局林产品检验检测中心(杭州) 浙江杭州310023

建立一种简单、灵敏的用于测定水果、蔬菜中氯丹残留量的气相色谱检测方法。样品用乙腈提取,经Florisil(弗罗里硅土)SPE小柱净化,由配有ECD检测器的气相色谱(GC-ECD)进行测定。该方法对水果、蔬菜中氯丹检出限为0.0002 mg/kg,工作曲线在... 详细信息

建立一种简单、灵敏的用于测定水果、蔬菜中氯丹残留量的气相色谱检测方法。样品用乙腈提取,经Florisil(弗罗里硅土)SPE小柱净化,由配有ECD检测器的气相色谱(GC-ECD)进行测定。该方法对水果、蔬菜中氯丹检出限为0.0002 mg/kg,工作曲线在0.001~0.08μg/mL范围内呈良好的线性关系。在该线性范围内,苹果、柿子、西红柿和鲜笋中回收率为82.6%~93.1%。

关键词：固相萃取氯丹残留分析气相色谱

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CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system

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Neural Regeneration Research 2025年第8期20卷 2420-2432页

作者： Lin Yin Yalan Xu jie Mu Yu Leng Lei Ma Yu Zheng Ruizhi Li Yin Wang Peifeng Li Hai zhu Dong Wang Jing Li Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina School of Basic Medicine Qingdao UniversityQingdaoShandong ProvinceChina College of Life Sciences and School of PharmacyMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina Department of Urology Qingdao Municipal Hospital Affiliated to Qingdao UniversityQingdaoShandong ProvinceChina

The protein connector enhancer of kinase suppressor of Ras 2(CNKSR2),present in both the postsynaptic density and cytoplasm of neurons,is a scaffolding protein with several protein-binding *** of the CNKSR2 gene have been implicated in neurodevelopmental disorders,particularly intellectual disability,although the precise mechanism involved has not yet been fully *** has demonstrated that CNKSR2 plays a role in facilitating the localization of postsynaptic density protein complexes to the membrane,thereby influencing synaptic signaling and the morphogenesis of dendritic ***,the function of CNKSR2 in the cytoplasm remains to be *** this study,we used immunoprecipitation and high-resolution liquid chromatography-mass spectrometry to identify the interactors of *** a combination of bioinformatic analysis and cytological experiments,we found that the CNKSR2 interactors were significantly enriched in the proteome of the *** also showed that CNKSR2 interacted with the microtubule protein DYNC1H1 and with the centrosome marker *** colocalization analysis confirmed the centrosomal localization of *** we downregulated CNKSR2 expression in mouse neuroblastoma cells(Neuro 2A),we observed significant changes in the expression of numerous centrosomal *** manipulation also affected centrosome-related functions,including cell size and shape,cell proliferation,and ***,we found that CNKSR2 interactors were highly enriched in de novo variants associated with intellectual disability and autism spectrum *** findings establish a connection between CNKSR2 and the centrosome,and offer new insights into the underlying mechanisms of neurodevelopmental disorders.

关键词： autism spectrum disorder centrosome CNKSR2 intellectual disability interactome mass spectrometry microtubule neurodevelopmental disease protein complexes protein-protein interactions

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逆行性可膨胀髓内钉治疗肱骨干骨折的临床应用

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中国骨伤 2009年第4期22卷 309-310页

作者：朱丹杰夏冰毕擎洪剑飞浙江省人民医院骨科浙江杭州310014

肱骨干骨折是临床常见的一种骨折类型，约占全身骨折的1．31％。肱骨干骨折的保守治疗已取得了满意的疗效，但随着内固定物材料的发展以及手术操作的日益完善，采用手术方法治疗肱骨干骨折已得到广泛认可。尤其对于多发骨折、开放骨折... 详细信息

肱骨干骨折是临床常见的一种骨折类型，约占全身骨折的1．31％。肱骨干骨折的保守治疗已取得了满意的疗效，但随着内固定物材料的发展以及手术操作的日益完善，采用手术方法治疗肱骨干骨折已得到广泛认可。尤其对于多发骨折、开放骨折、脊髓损伤（高位截瘫）或臂丛损伤、骨折伴有神经血管损伤、漂浮肘和闭合复位不满意的患者，手术治疗已经成为首选的治疗方法。白2005年9月至2007年10月，

关键词：肱骨骨折骨折固定术,髓内外科手术

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红树林植物叶片形态和解剖特征对叶肉导度、叶片导水率的影响

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植物科学学报 2016年第6期34卷 909-919页

作者：田尚青朱师丹朱俊杰申智骅曹坤芳广西大学林学院亚热带农业生物资源保护与利用国家重点实验室广西南宁530004

叶肉导度和叶片导水率是影响光合作用的两个重要过程,叶肉导度通过影响从气孔下腔到Rubisco酶位点的二氧化碳浓度梯度直接影响光合作用,而叶片导水率则通过影响水分供应或气孔行为来影响光合作用,然而对这两个生理过程之间的协同性研究... 详细信息

叶肉导度和叶片导水率是影响光合作用的两个重要过程,叶肉导度通过影响从气孔下腔到Rubisco酶位点的二氧化碳浓度梯度直接影响光合作用,而叶片导水率则通过影响水分供应或气孔行为来影响光合作用,然而对这两个生理过程之间的协同性研究较少。本研究选择9种红树林植物为研究对象,探讨盐生环境下植物叶肉导度和叶片导水率的协同性及其与叶片解剖结构特征之间的相关性。结果表明,9种红树林植物叶片导水率(0.78~5.83 mmol·m^(-2)·s^(-1)·MPa-1)、叶肉导度(0.06~0.36 mol·m^(-2)·s^(-1))、最大光合速率(7.23~23.71μmol·m^(-2)·s^(-1))等特征的差别较大;叶肉导度与最大光合速率呈显著正相关,而与比叶重无显著相关性,其原因是由于比叶重与叶片厚度、叶片密度不存在相关性;叶脉密度与气孔密度呈较强的相关性,说明红树林植物叶片水分运输与散失相关的叶片结构之间存在协同关系;叶片导水率不受叶脉密度影响,并且与叶肉导度、最大光合速率也不存在相关性,这很可能与红树林植物叶片的肉质化、有发达的储水组织有关,体现了红树林植物叶片结构和功能的特殊性。

关键词：红树林叶肉导度叶片导水率解剖特征光合速率

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