Effect of combination therapy with alginate dressing and mouse epidermal growth factor on epidermal stem cells in patients with refractory wounds

作     者：Bi Qing Zhang Qiong Ma Jun Xu Ming Zhang Shui-jun Qiu Bin-song Xia Bing Gu Hai-feng Hong Jian-fei Zhao Chen Zhu Dan-jie 

作者机构：Zhejiang Prov Peoples Hosp Dept Orthoped Hangzhou 310014 Zhejiang Peoples R China Zhejiang Prov Peoples Hosp Dept Operating Room Hangzhou 310014 Zhejiang Peoples R China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2012年第125卷第2期

页      面：257-261页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

主　　题：alginates epidermal growth factor stem cells wound healing 

摘      要：Background The aim of this research was to determine the efficacy of combination therapy using an alginate dressing and mouse epidermal growth factor （mEGF） on proliferation and differentiation of epidermal stem cells （ESCs） in patients with refractory *** Eighteen patients （12 males and 6 females,aged from 18 to 61 years （mean 36.4 years）） with various skin wounds,were treated by dressing changing for one *** wounds were located in the foot （11）,calf （3）,thigh （2）and forearm （2）.The patients were randomly divided into 3 groups：alginate dressing and mEGF （group A; n=6）,mEGF （group B; n=6） and control （group C; n=6）.Wound closure indexes were measured at 7,14,21 and 28 *** were harvested for pathologic examination,at 7 and 14 days following *** 10 （CK10） and cytokeratin 15 （CK15） positive cells were evaluated using the super-sensitivity （SP） immunohistochemical staining *** Wound healing was promoted in groups A and *** group A,the wound closure index was increased significantly （P 〈0.05）,and in one case the maximum cure area reached 102 *** examination identified a thicker epidermis,active angiogenesis and enhanced granulation in group A compared with groups B and *** the SP immunohistochemical staining technique,we showed that ESCs in group A were bigger in size and larger in number than in groups B and ***,there was a significant difference in ESCs proliferation and differentiation between group A and group B （or C）.Conclusions Combination therapy using an alginate dressing and mEGF shows increased proliferation and differentiation of ESCs in patients with refractory wounds compared with those treated with mEGF alone.