检索结果-南通市图书馆

A Comparative Study of Fibroblast Behaviors under Cyclic Stress Stimulus and Static Culture on 3D Patterned Matrix

在线全文

学校读者我要写书评

暂无评论

Journal of Bionic Engineering 2013年第2期10卷 148-155页

作者： Mingyan Zhao Lihua Li Bo Li Di Wu changren zhou Ye Tian Department of Materials Science and Engineering Jinan University Guangzhou 510630 P. R. China Engineering Research Center of Artificial Organs and Materials (Ministry of Education China) Guangzhou 510630 P. R. China Guangdong Medical Devices and Test Institute Guangzhou 510632 P. R. China

Mechanical stress and patterned surface of the scaffolds has been recognized as a crucial factor in determining cell func- tionality and tissue development, which in turn can direct the cell responses. In this study, fibroblasts M-3T3 in three-dimensional （3D） honeycomb patterning Chitosan/Poly（L-Lactic Acid）（CS/PLLA） composites was stimulated by a 15% sinusoidal （1 Hz） strain applied by a biodynamic test instrument. The effects of mechanical stimulus on the cell proliferation and basic Fibroblast Growth Factor （bFGF） secretion were studied in comparison to the non-strain groups and blank control. Results show that fibroblasts are able to sense the mechanical stimulation and respond, resulting in a time dependent increase of bFGF secretion and promoting cell proliferation. Moreover, the cells seeded in the scaffolds showed a higher cell proliferation and bFGF secretion. These findings support the hypothesis that suitable mechanical stimulus has positive effect on fibroblasts, and such a 3D honeycomb patterned scaffold may play a positive role in regulating cell behaviors in vitro.

关键词： polymer composites bFGF mechanical stimulus fibroblast

Subcellular localization of chitosan oligosaccharides in living cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2014年第20期59卷 2449-2454页

作者： Xian Li changren zhou Xiaofeng Chen Mingyan Zhao School of Materials Science and Engineering South China University of Technology National Engineering Research Center for Human Tissue Restoration and Reconstruction Department of Materials Science and Engineering Jinan University

Chitosan oligosaccharides(COS)is derived from the chemical or enzymatic decomposition of *** exact mechanisms underlying many biological functions of COS have not been *** subcellular localization is very important in determining biological activity in a number of molecules,we used fluorescein isothiocyanate-labeled chitosan oligosaccharides(FITCCOS)and investigated the localization of COS in living cells by laser scanning *** found that COS entered cells in a dose-and time-dependent manner,and we first showed that COS may enter cells by facilitated passive diffusion and was preferentially localized in the *** in high concentration,it was also found in the cytoplasm and nucleus and was enriched in the nucleolus and *** different subcellular localization of COS suggested that they played different *** of COS subcellular localization in cells is important to help us understand the potent mechanisms underlying its multiple functions.

关键词：亚细胞定位活细胞壳聚糖寡糖脱乙酰壳多糖激光扫描显微镜 COS 生物学功能

Protein adsorption behaviors on chitosan/poly(ε-caprolactone) blend films studied by quartz crystal microbalance with dissipation monitoring(QCM-D)

在线全文

学校读者我要写书评

暂无评论

Science China(Technological Sciences) 2009年第8期52卷 2275-2279页

作者： ZENG Rong ZHANG Yi LIANG ZhiHong TU Mei zhou changren Department of Materials Science and Engineering College of Science and EngineeringJinan UniversityGuangzhou 510632China Engineering Research Center of Artificial Organs and Materials Ministry of EducationGuangzhou 510632China Experiment and Technology Center Jinan UniversityGuangzhou 510632China

Chitosan/poly(ε-caprolactone) (PCL) blend films in different mass ratios were prepared using the chitosan/PCL mixture solutions in 80 vol.-% acetic acid by spin coating. Their surface micromorphologies were assessed by atomic force microscopy (AFM). It was found that the micromorphology of chitosan/PCL blend films was in large extent related to the mass ratio of chitosan. 25 wt% chitosan/PCL blend film presented microphase separation. The protein adsorption of bovine serum albumin (BSA) onto chitosan/PCL blend films was investigated by using quartz crystal microbalance with dissipation monitoring (QCM-D) in real time. The results suggested that the amount of adsorbed BSA on the chitosan/PCL blend films decreased with the addition of chitosan, but the structure and viscoelastic properties of the adsorbed BSA layers were greatly affected by the surface micromorphology of chitosan/PCL blend films. BSA absorbed on the 25 wt% chitosan/PCL blend film with microphase separa- tion showed larger adsorption reversibility, and preferred to form a loose, dissipative layer in comparison with those on other chitosan/PCL blend films without microphase separation.

关键词： protein adsorption chitosan poly(ε-caprolactone) quartz crystal microbalance with dissipation monitoring(QCM-D) surface micromorphology

Synthesis and Characterization of a Silica-Based Drug Delivery System for Spinal Cord Injury Therapy

在线全文

学校读者我要写书评

暂无评论

Nano-Micro Letters 2019年第2期11卷 61-80页

作者： Guodong Sun Shenghui Zeng Xu Liu Haishan Shi Renwen Zhang Baocheng Wang changren zhou Tao Yu College of Chemistry and Material Sciences Jinan UniversityGuangzhou 510632People’s Republic of China Department of Orthopedics First Affiliated HospitalJinan UniversityGuangzhou 510632People’s Republic of China College of Traditional Chinese Medicine Jinan UniversityGuangzhou 510632People’s Republic of China Tsinghua-Berkeley Shenzhen Institute Tsinghua UniversityShenzhen 518055People’s Republic of China

Acute inflammation is a central component in the progression of spinal cord injury(SCI).Anti-inflammatory drugs used in the clinic are often administered systemically at high doses,which can paradoxically increase inflammation and result in drug toxicity.A cluster-like mesoporous silica/arctigenin/CAQK composite(MSN-FC@ARCG)drug delivery system was designed to avoid systemic side effects of high-dose therapy by enabling site-specific drug delivery to the spinal *** this nanosystem,mesoporous silica was modified with the FITC fluorescent molecule and CAQK peptides that target brain injury and SCI *** size of the nanocarrier was kept at approximately 100 nm to enable penetration of the blood–brain ***,a Chinese herbal medicine,was loaded into the nanosystem to reduce *** in vivo results showed that MSN-FC@ARC-G could attenuate inflammation at the injury *** and morphology experiments suggested that MSN-FC@ARC-G could diminish local microenvironment damage,especially reducing the expression of interleukin-17(IL-17) and IL-17-related inflammatory factors,inhibiting the activation of astrocytes,thus protecting neurons and accelerating the recovery of *** study demonstrated that this novel,silica-based drug delivery system has promising potential for clinical application in SCI therapy.

关键词： Silica Drug delivery Spinal cord injury Arctigenin Astrocytes

Bio-inspired Cell Membrane Ingredient Cholesterol-conjugated Chitosan as a Potential Material for Bone Tissue Repair

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2016年第3期32卷 406-413页

作者： LI Zhenzhen WEN Jianhua JIA Weijian DING Shan XIA Xiaohui zhou changren HUANG Yadong Department of Materials Science & Engineering School of Science & Engineering Jinan University Guangzhou 510632 P. R. China Engineering Research Centre of Artificial Organs & Materials Jinan University Guangzhou 510632 P. R. China Institute of Biomedicine & National Engineering Research Centre of Genetic Medicine Jinan University Guangzhou 510632 P. R. China

We prepared a cholesterol-conjugated chitosan（CHCS） material and evaluated its potential application as a bone tissue repair material by in vitro cell experiments. Cell proliferation, differentiation and morphology on CHCS membrane surfaces with different graft degrees were assessed in mouse pre-osteoblasts MC3T3-E1 cells. The results indicate that CHCS materials could promote the proliferation of MC3T3-E1 cells at low graft degrees, but the CHCS material with high graft degree inhibits the proliferation of cells in contrast to the pure chitosan membrane. However, the alkaline phosphatase（ALP） activity of MC3T3-E1 ceils on different CHCS membrane surface increased with in- creasing graft degrees of cholesterol. The area of cells stretched onto the surface of CHCS materials was larger than on the surface of CS materials, and more microfilaments and stress fibers in cells were observed on CHCS materials than on the pure chitosan material surface. After 7 d, the expression of related osteogenic marker genes, such as rum-related transcription factor 2（Runx2）, osterix（OSX）, osteocalcin（OCN）, osteopontin（OPN）, ALP and collagen I（COL-I） were all up-regulated in CHCS materials to different degrees compared to pure chitosan material, which in- dicated that the CHCS materials facilitated MC3T3-EI cell differentiation and maturation, Characterizing CHCS materials is useful in designing and developing strategies for bone tissue engineering.

关键词： Bone repair material Chitosan Cholesterol MC3T3-E1 Cell differentiation

A thermostability perspective on enhancing physicochemical and cytological characteristics of octacalcium phosphate by doping iron and strontium

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2021年第5期6卷 1267-1282页

作者： Haishan Shi Xiaoling Ye Jing Zhang Tingting Wu Tao Yu changren zhou Jiandong Ye School of Stomatology Jinan UniversityGuangzhou510632China National Engineering Research Center for Tissue Restoration and Reconstruction South China University of TechnologyGuangzhou510006China School of Materials Science and Engineering South China University of TechnologyGuangzhou510640China College of Chemistry and Materials Science Jinan UniversityGuangzhou510632China National Engineering Research Center for Healthcare Devices Guangdong Institute of Medical InstrumentsGuangdong Academy of SciencesGuangzhou510500China

Investigation of thermostability will lead the groundbreaking of unraveling the mechanism of influence of ion-doping on the properties of calcium *** this work,octacalcium phosphate(OCP),a metastable precursor of biological apatite,was used as a stability model for doping ions(Fe^(3+)and Sr^(2+))with different ionic charges and *** treated under hot air at different temperatures(110-200◦C),the phase,morphology,structure,physicochemical properties,protein affinity,ions release,and cytological responses of the ion-doped OCPs were investigated *** results showed that the collapse of OCP crystals gradually occurred,accompanying with the dehydration of hydrated layers and the disintegration of plate-like crystals as the temperature *** collapsed crystals still retained the typical properties of OCP and the potential of conversion into *** to the undoped OCP,Fe-OCP,and Sr-OCP had lower and higher thermostability respectively,leading to different material surface properties and ions *** adjusted thermostability of Fe-OCP and Sr-OCP significantly enhanced the adsorption of proteins(BSA and LSZ)and the cytological behavior(adhesion,spreading,proliferation,and osteogenic differentiation)of bone marrow mesenchymal stem cells to a varying extent under the synergistic effects of corresponding surface characteristics and early active ions *** work paves the way for understanding the modification mechanism of calcium phosphates utilizing ion doping strategy and developing bioactive OCP-based materials for tissue repair.

关键词： Octacalcium phosphate Thermostability Iron Strontium Osteogenesis

Protein adsorption on the poly(L-lactic acid) surface modified by chitosan and its derivatives

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2009年第18期54卷 3167-3173页

作者： JIAO YanPeng zhou changren LI LiHua DING Shan LU Lu LUO BingHong LI Hong Department of Materials Science and Engineering Jinan University Guangzhou China 510632

Surface modification of biomaterials has been adopted over the years to improve their biocompatibility. In this study, aiming to promote hydrophilicity and to introduce natural recognition sites onto poly(L-lactic acid) (PLLA) films, chitosan and its derivatives, carboxymethyl chitosan(CMC) and N-methylene phosphonic chitosan (NPC), were used to modify the surface of PLLA films by an entrapment method. Radiolabeled (125I) proteins were used to measure the amount of protein adsorbed to PLLA surfaces. Fibronectin (Fn) was used to study the protein adsorption on the modified PLLA surfaces, including isotherm adsorption and adsorption kinetics of single protein, competitive adsorption of binary proteins system and serum multi-proteins and the desorption behavior in serum solution. The results showed that in the isotherm adsorption, Fn had a larger adsorption capacity on the CS-modified surface at lower concentrations, but had a high adsorption capacity at CMC-modified surface at higher concentrations. In the study of absorption kinetics, Fn had a fastest adsorption equilibrium and a highest equilibrium adsorption capacity at the CS-modified surface, while it was opposite at the PCS-modified surface. When BSA and serum were added, it had the greatest effect on the adsorption of Fn on the PCS-modified surface. After 6 hours soaking in the desorption study, Fn had reached desorption equilibrium on all the modified surfaces, which had different effects on the desorption rate and the remaining percentage of Fn.

关键词：材料表面改性蛋白质吸附改性壳聚糖聚乳酸衍生物羧甲基壳聚糖 125I标记纤维连接蛋白

Photothermal effective CeO_(2)NPs combined in thermosensitive hydrogels with enhanced antibacterial,antioxidant and vascularization performance to accelerate infected diabetic wound healing

在线全文

学校读者我要写书评

暂无评论

Regenerative Biomaterials 2023年第1期10卷 1372-1387页

作者： Zexiang Zheng Xing Yang Min Fang Jinhuan Tian Shuyun Zhang Lu Lu changren zhou Changpeng Xu Yong Qi Lihua Li College of Chemistry and Materials Science Engineering Research Center of Artificial Organs and MaterialsJinan UniversityGuangzhou 511486China Guangdong Second Provincial General Hospital Postdoctoral Research Station of Basic MedicineSchool of MedicineJinan UniversityGuangdong 510632PR China Department of Orthopaedics Guangdong Second Provincial General HospitalFaculty of Medical ScienceJinan UniversityGuangzhou 510317China

Chronic diabetic wound healing remains a formidable challenge due to susceptibility to bacterial infection,excessive oxidative stress,and poor *** address these issues,a sodium alginate(SA)based photothermal hydrogel dressing with multifunction was fabricated to facilitate wound *** nanoparticles(CeO_(2)NPs)was synthesized,and their antibacterial performance by near-infrared light triggered photothermal effects was first studied and verified in this *** addition,to release CeO_(2)NPs to achieve antioxidation and pro-vascularization,thermosensitive gelatin(Gel)was utilized to embed the nanoparticles in advance and then composited in SA hydrogel *** network was finally strengthened by acid soaking to form partially crystalline regions to act as natural *** showed that the Gel/SA/CeO_(2)hydrogel displayed temperature-responsive release of CeO_(2)NPs,significant antibacterial and antioxidative activity,as well as the ability to remove without injury and promote infected diabetic wound healing with low cytotoxicity,according to antibacterial investigations,cell studies,and in vivo animal *** research offers not only a successful method for quickening the healing of diabetic wounds but also a fresh approach to the general use of CeO_(2)NPs.

关键词： photothermal hydrogel sodium alginate cerium dioxide nanoparticle gelatin infected diabetic wound healing

Effect of chitooligosaccharides on cyclin D1,bcl-xl and bcl-2 mRNA expression in A549 cells using quantitative PCR

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2011年第15期56卷 1629-1632页

作者： LI Xian WANG Ju CHEN XiaoJia TIAN JinHuan LI LiHua ZHAO MingYan JIAO YanPeng zhou changren Department of Materials Science and Engineering Jinan University Guangzhou 510632 China1 Bioengineering Institute Jinan University Guangzhou 510632 China

Chitooligosaccharide (COS) is derived from the chemical and enzymatic hydrolysis of chitosan and has been reported to have potent antitumor *** study investigated the effects of five chitooligomers ranging from the dimer form to the hexamer form (chitobiose,chitotriose,chitotetraose,chitopentaose,chitohexaose) on the expression of cyclin D1,bcl-2 and bcl-xl mRNA in A549 cells using reverse transcription quantitative real-time *** demonstrated that,of the five chitooligomers used,chitohexaose (COS6) had the most potent inhibitory effect on A549 cell ***6 also significantly down regulated cyclin D1 and bcl-xl mRNA expression *** data suggested that COS6 exerts its antitumor activity by two different mechanisms:(1) COS6-mediated inhibition of Cyclin D1 levels leads to suppression of tumor cell proliferation;and (2) COS6-mediated down-regulation of the pro-survival protein,Bcl-xl,promotes the apoptosis of tumor cells.

关键词：细胞周期蛋白D1 mRNA表达水平 A549细胞壳寡糖定量PCR 肿瘤细胞凋亡抗肿瘤活性 cyclin