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Differential expression and function prediction of miRNAs in Hepatocellular carcinoma based on bioinformatics

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Journal of Hainan Medical University 2020年第10期26卷 38-41页

作者： Cheng-zhou pa Shan-Min Wu Department of Hepatobiliary Surgery Renmin Hospital of Wuhan University wuhan 430060 China

Objective:To analyze the differential expression and function prediction of miRNAs in Hepatocellular carcinoma (HCC) based on Bioinformatics, and to provide new ideas for early diagnosis and late intervention of ***: The expression dataset of HCC non-coding RNA was obtained by GEO database, and differential miRNAs were screened and visualized by limma R package and pheatmap R package using R (v 3.6.0). Transcriptional factors of differential miRNAs were predicted, signaling pathway analysis and GO enrichment analysis were performed and ***: A total of 37 miRNAs were selected in HCC tissues with differential expressions, and the significant transcription factors were EGR1, POU2F1, SP1, NKX6-1, MEF2A, FOXA1, SP4, NFIC, RREB1, ARID3A, HOXA5, HNF4A, HOXD8, RORA, LHX3, POU3F2, FOXJ2, POU5F1, NKX2-1, SOX1. Differential miRNAs were found to be mainly located in the Nucleus and Cytoplasm, It has molecular functions such as Protein serine/threonine kinase activity, Receptor signaling complex scaffold activity, Transcription regulator activity and Transcription factor activity, Involved in Nucleic acid metabolism and Signal transduction and Cell communication. Through The epidermal growth factor receptor (EGFR), IGF-1,γ-IFN, Plasma membrane estrogen receptor signaling pathways play a role, and differential miRNAs is associated with VEGF, VEGFR, PDGF, Hepatocyte Growth Factor Receptor (c-Met), Protein serine/threonine kinase mediated signaling ***:Differential mirnas in hepatocellular carcinoma are closely related to tumor development and progression, and differential miRNAs can be used as potential markers for early diagnosis of HCC, providing new ideas for late intervention.

关键词： Hepatocellular carcinoma MiRNA GEO

A matrix metalloproteinase-responsive hydrogel system controls angiogenic peptide release for repair of cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2025年第2期20卷 503-517页

作者： Qi Liu Jianye Xie Runxue zhou Jin Deng Weihong Nie Shuwei Sun Haiping Wang Chunying Shi Department of Human Anatomy Histology and EmbryologySchool of Basic MedicineQingdao UniversityQingdaoShandong ProvinceChina Department of Neurology The Affiliated Hospital of Qingdao UniversityQingdaoShandong ProvinceChina Department of General Practice The Affiliated Hospital of Qingdao UniversityQingdaoShandong ProvinceChina

Vascular endothelial growth factor and its mimic peptide KLTWQELYQLKYKGI(QK)are widely used as the most potent angiogenic factors for the treatment of multiple ischemic ***,conventional topical drug delivery often results in a burst release of the drug,leading to transient retention(inefficacy)and undesirable diffusion(toxicity)in ***,a drug delivery system that responds to changes in the microenvironment of tissue regeneration and controls vascular endothelial growth factor release is crucial to improve the treatment of ischemic *** metalloproteinase-2(MMP-2)is gradually upregulated after cerebral ***,vascular endothelial growth factor mimic peptide QK was self-assembled with MMP-2-cleaved peptide PLGLAG(TIMP)and customizable peptide amphiphilic(pa)molecules to construct nanofiber hydrogel ***-TIMP-QK was found to control the delivery of QK by MMP-2 upregulation after cerebral ischemia/reperfusion and had a similar biological activity with vascular endothelial growth factor in *** results indicated that pa-TIMP-QK promoted neuronal survival,restored local blood circulation,reduced blood-brain barrier permeability,and restored motor *** findings suggest that the self-assembling nanofiber hydrogel pa-TIMP-QK may provide an intelligent drug delivery system that responds to the microenvironment and promotes regeneration and repair after cerebral ischemia/reperfusion injury.

关键词： angiogenesis biomaterial blood-brain barrier cerebral ischemia/reperfusion injury control release drug delivery inflammation QK peptides matrix metalloproteinase-2 neuroprotection self-assembling nanofiber hydrogel

Comprehensive profiling of CTP-binding proteins using a biotinylated CTP affinity probe

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Chinese Chemical Letters 2021年第11期32卷 3479-3482页

作者： Mengting pa Yunming Liu Xiaofang Zheng Meijuan zhou Changjun You Xiaoxia Dai State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringMolecular Science and Biomedicine LaboratoryHunan Provincial Key Laboratory of Biomacromolecular Chemical BiologyHunan UniversityChangsha 410082China

Recent studies have shown that CTP may act as a ligand to regulate the activity of its target proteins in many biological ***,proteome-wide identification of CTP-binding proteins remains ***,we employed a biotinylated CTP affinity probe coupled with stable isotope labeling by amino acids in cell culture(SILAC)-based quantitative proteomics approach to capture,identify and quantify CTP-binding proteins in human *** performing two types of competitive SILAC experiments with high *** concentrations of CTP probe(100 vs.10µmol/L)or with CTP probe in the presence of free CTP,we identified 90 potential CTP-binding proteins which are involved in a variety of biological processes,including protein folding,nucleotide binding and cell-cell ***,we developed a chemical proteomic method for uncovering the CTP-binding proteins in human cells,which could be widely applicable for profiling CTP-binding proteins in other biological samples.

关键词： CTP-binding proteins CTP affinity probe Mass spectrometry SILAC Quantitative proteomics

Design of Slant-Form Tool in Precision Reuse of Digital paper Display

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Journal of Materials Science & Technology 2011年第9期27卷 846-850页

作者： pai-Shan pa Department of Digital Content Design Graduate School of Toy and Game Design National Taipei University of Education Taipei 10667 Taiwan China

A reuse fabrication module using micro electroetching as a precision machining process with a new design of a slant-form tool to remove the defective indium-tin-oxide （ITO） nanostructure from the optical polyethyleneterephthalate （PET） surfaces of digital paper display is presented in current studies. The low yield of ITO thin film deposition is an important factor in optoelectronic semiconductor production. The adopted precision reuse process requires only a short period of time to remove the ITO nanostructure easily and cleanly, which is based on technical and economical considerations and is highly efficient. In the current experiment, a large inclined angle of the cathode and a small end radius of the anode take less time for the same amount of ITO removal. A higher feed rate of the optical PET diaphragm combines with enough electric power to drive fast micro electroetching. A small rotational diameter of the anode accompanied by a small width of the cathode corresponds to a higher removal rate for the ITO nanostructure. A pulsed direct current can improve the effect of dreg discharge and is advantageous to couple this current with the fast feed rate of the workpiece. This improvement is associated with an increase in current rating. High rotational speed of the slant-form tool can improve the effect of dregs discharge and is advantageous to associate with the fast feed rate of the workpiece （optical PET diaphragm）.

关键词： Reuse fabrication Slant-Form Digital paper display Optical polyethyleneterephthalate (PET) diaphragm Indium-tin-oxide (ITO) nanostructure Micro electroetching

Selective removal technology using chemical etching and excimer assistance in precision recycle of color filter

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Transactions of Nonferrous Metals Society of China 2011年第A01期21卷 210-214页

作者： pai-shan pa Digital Content Design Graduate School of Toy and Game Design National Taipei University of Education Taipei City 10671 China

Color filters are produced using semiconductor production techniques although problems with low yield remain to be addressed. This study presents a new means of selective removal using excimer irradiation, chemical etching, or electrochemical machining on the fifth generation TFT LCDs. The selective removal of microstructure layers from the color filter surface of an optoelectronic flat panel display, as well as complete removal of the ITO thin-films, RGB layer, or resin black matrix (BM) layer from the substrate is possible. Individual defective film layers can be removed, or all films down to the Cr layer or bare glass can be completely eliminated. Experimental results demonstrate that defective ITO thin-films, RGB layers, or the resin BM layer can now be recycled with a great precision. When the ITO or RGB layer proves difficult to remove, excimer light can be used to help with removal. During this recycling process, the use of 225 nm excimer irradiation before chemical etching, or electrochemical machining, makes removal of stubborn film residues easy, effectively improving the quality of recycled color filters and reducing fabrication cost.

关键词： chemical etching excimer light selective removal technology display color filter

An effective round ball tool in electrochemistry process as novel reuse fabrication technology

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中国有色金属学会会刊：英文版 2012年第S3期22卷 825-830页

作者： P.S.pa Department of Digital Content Design Graduate School of Toy and Game DesignNational Taipei University of Education

A reuse fabrication module using micro electrochemistry (MECM) with a round-ball tool to remove the defective In2O3 SnO2 thin film from the surfaces of digital paper display was *** etching effect improves that the number of the round-balls decreases for promoting the concentration of electric power and increasing discharge *** a small size of the round-ball tool takes less time for the same amount of In2O3 SnO 2 layer removal since the effect of MECM is easily developed for supplying of sufficient electrochemical power.A higher feed rate of the poly ethylene terephthalate (PET) diaphragm combines with enough electric power to drive fast etching rate.A pulsed direct current can improve the effect of dreg discharge and is advantageous to couple this current with the fast feed rate of the *** the ultra-precise etching of In2O 3 SnO2,the optoelectronic semiconductor industry can effectively reuse the defective products,reducing production *** precision etching process is of high efficiency and requires only a short period of time to remove the In2O3 SnO2 nanostructures.

关键词： thin film round-ball tool In2O3SnO2 micro electrochemistry digital paper display

Effective tool design of three-rank form as precision removal-process of ITO thin-films

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中国有色金属学会会刊：英文版 2009年第B09期19卷 232-237页

作者： pai-shan pa Digital Content Design Graduate School of Toy and Game DesignNational Taipei University of Education

A new effective tool design of three-rank form of electroremoval was present using a precision recycle system offering faster performance in removing the indium-tin-oxide(ITO) thin-films on color filter surface of displays. Higher electric power is not required since the three-rank form tool is adopted as a feeding mode to reduce the response area. The low yield of ITO persists throughout the entire semiconductor production process. By establishing a recycle process of ultra-precise removal of the thin-film nanostructure, defective products in the optoelectronic semiconductors industry can be effectively recycled, decreasing both production costs and pollution. A 5th generation TFT-LCD was used. The design features of the removal processes for the thin-films and the tool design of three-rank form were of major interest. For the precision removal processes, a pulsed current can improve the effect of dreg discharge and contributes to the achievement of a fast workpiece (displays' color filter) feed rate, but raises the current rating. High flow velocity of the electrolyte with a high rotational speed of the tool electrodes elevates the ITO removal effect. A displays' color filter with a fast feed rate is combined with enough electric power to provide highly effective removal. A small thickness of the rank and a small arc angle of the negative-electrode correspond to a higher removal rate for ITO-film. An effective three-rank form negative-electrode provides larger discharge mobility and better removal effect. It only needs a short period of time to remove the ITO easily and cleanly.

关键词：工具设计薄膜材料 ITO

Fusion expression of pedA gene to obtain biologically active pediocin pa-1 in Escherichia coli

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第1期12卷 65-71页

作者： Shan-na LIU Ye HAN Zhi-jiang zhou School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China

Two heterologous expression systems using thioredoxin (trxA) as a gene fusion part in Escherichia coli were developed to produce recombinant pediocin *** pa-1 structural gene pedA was isolated from Pediococcus acidilactici pa003 by the method of polymerase chain reaction (PCR),then cloned into vector pET32a(+),and expressed as thioredoxin-PedA fusion protein in the host strain *** BL21 (DE3).The fusion protein was in the form of inclusion body and was refolded before purification by nickel-iminodiacetic acid (Ni-IDA) agarose resin *** activity of recombinant pediocin pa-1 was analyzed after cleavage of the fusion protein by *** diffusion test revealed that 512-arbitrary unit (AU) recombinant pediocin pa-1 was obtained from 1 ml culture medium of *** (ppa003PED1) using Listeria monocytogenes as the indicator ***-PedA fusion gene was further cloned into pET20b(+).Thioredoxin-PedA fusion protein was detected in both the periplasmic and cytoplasmic *** recombinant pediocin pa-1 from the soluble fraction attained 384 AU from 1 ml culture medium of *** (ppa003PED2).Therefore,biologically active pediocin pa-1 could be obtained by these two hybrid gene expression methods.

关键词： Bacteriocin Fusion expression Inclusion body Pediocin pa-1 Thioredoxin

On the oxidation states of metal elements in MO-3(M=V,Nb,Ta,Db,Pr,Gd,pa)anions

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Science China Chemistry 2016年第4期59卷 442-451页

作者： Jing Su Shuxian Hu Wei Huang Mingfei zhou Jun Li Key Laboratory of Organic Optoelectronics and Molecular Engineering of Ministry of Education Department of ChemistryTsinghua UniversityBeijing 100084China Collaborative Innovation Center of Chemistry for Energy Materials Shanghai Key Laboratory of Molecular Catalysts and Innovative Materials Department of ChemistryFudan UniversityShanghai 200433China

Relativistic quantum chemistry investigations are carried out to tackle the puzzling oxidation state problem in a series of MO3 trioxide anions of all d- and f-block elements with five valence electrons. We have shown here that while the oxidation states of V, Nb, Ta, Db, pa are, as usual, all ＋V with divalent oxygen O（-II） in MO3- anions, the lanthanide elements Pr and Gd cannot adopt such high ＋V oxidation state in similar trioxide anions. Instead, lanthanide element Gd retains its usual ＋III oxi- dation state, while Pr retains a ＋IV oxidation state, thus forcing oxygen into a non-innocent ligand with an uncommon mono- valent radical （O＇） of oxidation state -I. A unique Pr＂- ＂（0）3 biradical with highly delocalized unpairing electron density on Pr（IV） and three O atoms is found to be responsible for stabilizing the monovalent-oxygen species in PRO3- ion, while GdO3 ion is in fact an OGd＋（O22-） complex with Gd（III）. These results show that a naive assignment of oxidation state of a chemical element without electronic structure analysis can lead to erroneous conclusions.

关键词： oxidation state non-innocent ligand biradical lanthanide trioxide anion