检索结果-南通市图书馆

Folding patterns of chromatin in chicken erythrocyte studied by using AFM and TEM

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1998年第4期43卷 289-291页

作者： Liu, ZX zhou, my Xie, HY Qian, RL Zhang, Y Hu, J Li, MQ Chinese Acad Sci Shanghai Inst Cell Biol Shanghai 200031 Peoples R China Chinese Acad Sci Shanghai Inst Nucl Res Shanghai 201800 Peoples R China

Four folding patterns (～15, ～30-40, ～60 and ～90-110 nm) of the higher_order structure of chromatin in chicken erythrocytes have been observed with an atomic force microscope (AFM) under near natural conditions. Results showed that the chromatin in the interphase nucleus of chicken erythrocyte was not in a disorganized form but represented a higher_order conformation. The thin sections of the chromatin in the nucleus of chicken erythrocyte were examined with a transmission electron microscope (TEM). Compared with TEM, AFM had the advantages that the samples did not need the preparation procedures such as fixing, sectioning and staining, and thus were closer to their native condition.

关键词： chromatin structure nucleus of chicken erythrocyte AFM TEM.

Changing trends of surgical treatment of hilar bile duct cancer:clinical and experimental perspectives

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2000年第6期6卷 777-782页

作者： Huang ZQ zhou NX Wang DD Lu JG Chen my Gen Hosp PLA Res Inst Gen Surg Beijing 100853 Peoples R China

HISTORICAL POINT OF VIEWCarcinoma of the confluence of the hepatic ductshas been thought to be a rare disease until *** of the large hepatic ducts was rarelydiagnosed correctly *** of itsdeeply seated location,resecti... 详细信息

关键词： bile duct neoplasms/surgery liver neoplasms/surgery bile duct neoplasms/pathology gene suppressor tumor immunohistochemistry polymerase chain reaction gene therapy gene expression portoenterostomy hepatic

Stabilization of an axially moving tape system with boundary disturbances via the ADRC approach

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Control Theory and Technology 2022年第3期20卷 349-360页

作者： my Driss Aouragh Mohamed Nahli Maths Department AM2CSI GroupMSISI LaboratoryFST ErrachidiaMoulay Ismail University of MeknesPO.BOX 509 BoutalamineErrachidia52000Drâa-TafilaletMorocco AM2CSI Group MSISI LaboratoryFST ErrachidiaMoulay Ismail University of MeknesPO.BOX 509 BoutalamineErrachidia52000Drâa-TafilaletMorocco

In this paper,the boundary stabilization problem of an axially moving tape system is *** tape moves between two sets of rollers,where the right roller is fixed and the left roller,with its mass taken into account,is free to move,rotate,and subject to external *** active disturbance rejection control approach is adopted in the ***,extended state observers are designed to estimate the disturbances,and then,feedback controllers are proposed to cancel the effect of the *** well-posedness of the closed-loop system is proved by the semigroup ***,the exponential stability is achieved by constructing a suitable Lyapunov ***,numerical simulations are given to support these results.

关键词： Axially moving beam Hybrid system Active disturbance rejection control Boundary disturbances Exponential stabilization

Cloning of murine BRI3 gene and study on its function for inducing cell death

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2002年第5期47卷 375-378,441页

作者： Lin, LZ Yu, H Ying, my Tang, J zhou, W Zhao, SY Li, CB Fudan Univ Sch Life Sci Inst Genet State Key Lab Genet Engn Shanghai 200433 Peoples R China

To understand the molecular mechanism of TNFα effects, the cDNA of murine BRI3 gene was cloned from the total RNA of murine brain endothelial cells (bEnd.3) treated with hTNFa by using the suppression subtractive hybridization (SSH) and the RT-PCR method. The fusion expression vector harbouring BRI3 gene and enhanced green fluorescence protein (EGFP) thus obtained were designated as pEGFP/I3. Then pEGFP/I3 was transiently transfected into L929 cells and the fusion protein EGFP/I3 was localized in cytoplasm. It is found that the expression of EGFP/I3 could induce cell death in L929 cells detected by TUNEL method and flow cytometry. And the overexpression of Bcl-2 in L929 cells can block cell death induced by EGFP/I3, indicating that murine BRI3 gene might related to the TNFα mediated cytotoxicity.

关键词： tumor necrosis factor α BRI3 fusion protein cell death.

A tribute to my supervisor Professor Zhengyi Wu

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2018年第10期9卷 827-831页

作者： Zhekun zhou Key Laboratory of Tropical Forest Ecology Xishuangbanna Tropical Botanical Garden Chinese Academy of Sciences Menglun Mengla 666303 Yunnan China Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of Botany Chinese Academy of Sciences Kunming 650201 China

I was saddened and quite shocked when I received the phone called informing me that Professor Zhengyi Wu had died. It was the evening of June 20th, 2013. I had just got off a plane from Tibet and turned on my mobile phone. Prof. Wu had been unwell for neady a year. I knew that this would happen one day, but refused to believe it would really come to pass. A few days ago, some students and I were on a field trip in Tibet. Sitting in our jeep, I watched the mountains and roadside as we drove, and tried to identify the plants along the route. The mountain roads in Tibet seem to always make my students sleepy. I woke them up.

关键词：

ENDOTOXIN REMOVAL BY MEMBRANE AFFINITY CHROMATOGRAPHY

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1995年第10期6卷 919-922页

作者： Guo, W Shang, ZH Yu, YN zhou, LM Zun, ZY Ma, LR Chen, my Chinese Acad Sci Dalian Inst Chem Phys Dalian 116012 Peoples R China Acad Mil Med Sci Inst Radiat Med Beijing 100850 Peoples R China

Endotoxin was removed by membrane affinity chromatography. Macropore cellulose membrane was prepared from filter paper by alkaline treatment and chemical crosslinking, and was used as stationary phase for affinity chromatography. The matrix membrane was derived by hexamethylenediamine and activated by glutaraldehyde before histidine was immobilized as affinity ligand. A membrane cartridge containing 40 sheets of affinity membrane was also prepared, which can be used to remove pyrogen from aqueous solutions.

关键词：

A graph-based approach for the structural analysis of road and building layouts

在线全文

学校读者我要写书评

暂无评论

Geo-Spatial Information Science 2019年第1期22卷 59-72页

作者： Mathieu Domingo Rémy Thibaud Christophe Claramunt Department of Computer Science University of Pau and Pays de l’AdourPauFrance Naval Academy Research Institute Brest CedexFrance

A better understanding of the relationship between the structure and functions of urban and suburban spaces is one of the avenues of research still open for geographical information *** research presented in this paper develops several graph-based metrics whose objective is to characterize some local and global structural properties that reflect the way the overall building layout can be cross-related to the one of the road *** structural properties are modeled as an aggregation of parcels,buildings,and road *** introduce several computational measures(Ratio Minimum Distance,Minimum Ratio Minimum Distance,and Metric Compactness)that respectively evaluate the capability for a given road to be connected with the whole road *** measures reveal emerging sub-network structures and point out differences between less-connective and moreconnective parts of the *** on these local and global properties derived from the topological and graph-based representation,and on building density metrics,this paper proposes an analysis of road and building layouts at different levels of *** metrics developed are applied to a case study in which the derived properties reveal coherent as well as incoherent neighborhoods that illustrate the potential of the approach and the way buildings and roads can be relatively connected in a given urban ***,and by integrating the parcels and buildings layouts,this approach complements other previous and related works that mainly retain the configurational structure of the urban network as well as morphological studies whose focus is generally limited to the analysis of the building layout.

关键词： Urban and suburban spaces graph-based modeling structural analysis GIS

High Manganese Steel Alloying Process and Its Influence on Microstructure and Properties of the Steel

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 1995年第2期11卷 102-108页

作者： SHI, ZL GU, my LIU, JY YIN, YS HEBEI INST MECH ELECT DEPT MAT SCI & ENGNSHIJIAZHUANGPEOPLES R CHINA DALIAN UNIV TECHNOL DEPT MAT SCI & ENGNDALIAN 116023PEOPLES R CHINA

The influence of two kinds of alloying processes, adding Nb (or Ti) and N-Mn alloy as well as adding Nb (or Ti) and spraying Nz, on microstructures and properties of a high manganese steel has been studied. It has been found that adding Nb(or Ti), accompanying with N-Mn alloy, is unfavourable to microstructure compactness of the high manganese steel, but adding Nb (or Ti) and spraying N-2 into the melt is good for refining austenitic grain, forming a lot of hard particles and improving microstructure compactness. The mechanical properties of the high manganese steel have relation to the content of elements Nb or Ti. Its fracture mode will turn ductile fracture into brittle cleavage fracture gradually By X-ray and TEM analysis, it is proved that the austenite can be transformed to deformation-induced cr martensite after adding a certain amount of element Nb (or Ti). The microstructure transformation of alloying high manganese steels through deformation is one of methods for strengthening austenite matrix acid increasing the work-hardening rate as well as improving antiwear property.

关键词： Alloying

Development of a Peristaltic Micropump for Bio-Medical Applications Based on Mini LIPCA

在线全文

学校读者我要写书评

暂无评论

Journal of Bionic Engineering 2008年第2期5卷 135-141页

作者： Thanh Tung Nguyen my Pham Nam Seo Goo Department of Aerospace Engineering Konkuk University Department of Advanced Technology Fusion Konkuk University

This paper presents the design, fabrication, and experimental characterization of a peristaltic micropump. The micropump is composed of two layers fabricated from Polydimethylsiloxane （PDMS） material. The first layer has a rectangular channel and two valve seals. Three rectangular mini lightweight piezo-composite actuators are integrated in the second layer, and used as actuation parts. Two layers are bonded, and covered by two Polymethyl Methacrylate （PMMA） plates, which help increase the stiffness of the micropump. A maximum flow rate of 900μ*** 1 and a maximum backpressure of 1.8 kPa are recorded when water is used as pump liquid. We measured the power consumption of the micropump. The micropump is found to be a promising candidate for bio-medical application due to its bio-compatibility, portability, bidirectionality, and simple effective design.

关键词： peristaltic micropump LIPCA PDMS bio-medical applications