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Improvement of Film Cooling Design for Turbine Vane Leading Edge Considering Combustor Outflow

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Journal of Thermal Science 2024年第1期33卷 311-327页

作者： WANG Xinyu LIU Cunliang FU zhongyi LI yang ZHU Huiren School of Power and Energy Northwestern Polytechnical UniversityXi'an 710072China AECC Hunan Aviation Powerplant Research Institute Zhuzhou 412002China

As the interaction between the combustor and the turbine in the aero-engine continues to increase,the film cooling design considering the combustor swirling outflow has become the research focus.The swirling inflow and high-temperature gas first affect the vane leading edge(LE).However,no practical improved solution for the LE cooling design has been proposed considering the combustor swirling outflow.In this paper,the improved scheme of showerhead cooling is carried out around the two ways of adopting the laid-back-fan-shaped hole and reducing the coolant outflow angle.The film cooling effectiveness(η) and the coolant flow state are obtained by PSP(pressure-sensitive-paint) and numerical simulation methods,respectively.The research results show that the swirling inflow increases the film distribution inhomogeneity by imposing the radial pressure gradient on the vane to make the film excessively gather in some positions.The showerhead film cooling adopts the laid-back-fan-shaped hole to reduce the momentum when the coolant flows out.Although this cooling scheme improves the film attachment and increases the surface-averaged film cooling effectiveness(η_(sur)) by as much as15.4%,the film distribution inhomogeneity increases.After reducing the coolant outlet angle,the wall-tangential velocity of the coolant increases,and the wall-normal velocity decreases.Under the swirl intake condition,both ηand the film distribution uniformity are significantly increased,and the growth of η_(sur) is up to 16.5%.This paper investigates two improved schemes to improve the showerhead cooling under the swirl intake condition to provide a reference for the vane cooling design.

关键词： turbine vane showerhead cooling swirling inflow laid-back-fan-shaped hole film hole inclination

Design of an automated robotic microinjection system for batch injection of zebrafish embryos and larvae

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Microsystems & Nanoengineering 2024年第1期10卷 89-103页

作者： zhongyi Guo Nana Ai Wei Ge Qingsong Xu Department of Electromechanical Engineering Faculty of Science and TechnologyUniversity of MacaoMacaoChina Department of Biomedical Sciences and Centre of Reproduction Development and Aging(CRDA)Faculty of Health SciencesUniversity of MacaoMacaoChina

This paper presents the design of a vision-based automated robotic microinjection system for batch injection of both zebrafish embryos and larvae.A novel visual recognition algorithm based on an automatic threshold and excessive dilatation is introduced to accurately identify the center of zebrafish embryos and larval yolks.A corresponding software system is developed using the producer-consumer model as the framework structure,and a friendly user interface is designed to allow operators to choose from a range of desired functions according to their different needs.In addition,a novel microstructural agarose device is designed and fabricated to simultaneously immobilize mixed batches of embryos and larvae.Moreover,a prototype microinjection system is fabricated by integrating hardware devices with visual algorithms.An experimental study is conducted to verify the performance of the robotic microinjection system.The results show that the reported system can accurately identify zebrafish embryos and larvae and efficiently complete batch microinjection tasks of the mixtures with an injection success rate of 92.05%in 13.88 s per sample.Compared with manual and existing microinjection systems,the proposed system demonstrates the merits of versatility,excellent efficiency,high success rate,high survival rate,and sufficient stability.

关键词： zebra automated hardware

An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica

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Journal of Oceanology and Limnology 2024年第1期42卷 216-225页

作者： zhongyi ZHANG Hang LIU Xiaohui PAN Yanan ZONG Leili FENG Lixian LIU Li GUO Guanpin yang College of Marine Life Sciences Ocean University of ChinaQingdao 266003China Institutes of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of ChinaQingdao 266003China

Insertional mutation,phenotypic evaluation,and mutated gene cloning are widely used to clone genes from scratch.Exogenous genes can be integrated into the genome during non-homologous end joining(NHEJ)of the double-strand breaks of DNA,causing insertional mutation.The random insertional mutant library constructed using this method has become a method of forward genetics for gene cloning.However,the establishment of a random insertional mutant library requires a high transformation efficiency of exogenous genes.Many microalgal species show a low transformation efficiency,making constructing random insertional mutant libraries difficult.In this study,we established a highly efficient transformation method for constructing a random insertional mutant library of Nannochloropsis oceanica,and tentatively tried to isolate its genes to prove the feasibility of the method.A gene that may control the growth rate and cell size was identified.This method will facilitate the genetic studies of N.oceanica,which should also be a reference for other microalgal species.

关键词： Nannochloropsis oceanica genetic transformation random insertional mutant library zeocin pretreatment forward genetics

RepBoTNet-CESA:An Alzheimer’s Disease Computer Aided Diagnosis Method Using Structural Reparameterization BoTNet and Cubic Embedding Self Attention

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Computers, Materials & Continua 2024年第5期79卷 2879-2905页

作者： Xiabin Zhang zhongyi Hu Lei Xiao Hui Huang College of Computer Science and Artificial Intelligence Wenzhou UniversityWenzhou325035China Key Laboratory of Intelligence Image Processing and Analysis Wenzhou UniversityWenzhou325035China

Various deep learning models have been proposed for the accurate assisted diagnosis of early-stage Alzheimer’s disease(AD).Most studies predominantly employ Convolutional Neural Networks(CNNs),which focus solely on local features,thus encountering difficulties in handling global features.In contrast to natural images,Structural Magnetic Resonance Imaging(sMRI)images exhibit a higher number of channel dimensions.However,during the Position Embedding stage ofMulti Head Self Attention(MHSA),the coded information related to the channel dimension is disregarded.To tackle these issues,we propose theRepBoTNet-CESA network,an advanced AD-aided diagnostic model that is capable of learning local and global features simultaneously.It combines the advantages of CNN networks in capturing local information and Transformer networks in integrating global information,reducing computational costs while achieving excellent classification performance.Moreover,it uses the Cubic Embedding Self Attention(CESA)proposed in this paper to incorporate the channel code information,enhancing the classification performance within the Transformer structure.Finally,the RepBoTNet-CESA performs well in various AD-aided diagnosis tasks,with an accuracy of 96.58%,precision of 97.26%,and recall of 96.23%in the AD/NC task;an accuracy of 92.75%,precision of 92.84%,and recall of 93.18%in the EMCI/NC task;and an accuracy of 80.97%,precision of 83.86%,and recall of 80.91%in the AD/EMCI/LMCI/NC task.This demonstrates that RepBoTNet-CESA delivers outstanding outcomes in various AD-aided diagnostic tasks.Furthermore,our study has shown that MHSA exhibits superior performance compared to conventional attention mechanisms in enhancing ResNet performance.Besides,the Deeper RepBoTNet-CESA network fails to make further progress in AD-aided diagnostic tasks.

关键词： Alzheimer CNN structural reparameterization multi head self attention computer aided diagnosis

Two-Dimensional Conductive Metal-Organic Framework Reinforced Spinterface in Organic Spin Valves

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CCS Chemistry 2024年第1期6卷 208-217页

作者： Xiaoyu Song Chao Jin Hongliang Chen Shuaishuai Ding zhongyi Liu yang Li Xiaoli Yan Wenbo Mi Long Chen Wenping Hu Department of Chemistry Tianjin Key Laboratory of Molecular Optoelectronic ScienceTianjin UniversityTianjin 300072 School of Chemistry and Chemical Engineering Yantai UniverisityYantaiShandong 264005 Tianjin Key Laboratory of Low Dimensional Materials Physics and Processing Technology School of ScienceTianjin UniversityTianjin 300072 State Key Laboratory of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchunJilin 130012

Interface engineering in device fabrication is a significant but complicated issue.Although great successes have been achieved by conventional physical in situ or ex situ methods,it still suffers from complicated procedures.In this work,we present a facile method for fabricating phthalocyanine(Pc)-based two-dimensional conductive metal–organic framework(MOF)films.Based on PcM-Cu(M=Ni,Cu,H_(2))MOF films,spin valves with a vertical configuration of La_(0.67)Sr_(0.33)MnO_(3)/PcM-Cu MOFs/Co were constructed successfully,and exhibited notably high negative magnetoresistance(MR)up to -22% at 50 K.The penetrated Co atoms coordinated with the dehydrogenated hydroxy groups in the MOFs resulting in an antiferromagnetic layer of the PcM-Cu-Co hybrid structure.Interestingly,a significant exchange bias effect was demonstrated at the PcM-Cu MOF/Co interface,beneficial for the MR behavior.Thus,our present study provides new insights into developing high-performance organic spin valves via de novo molecular design.

关键词： two-dimensional conductive metal-organic framework two-dimensional polymer phthalocyanine spintronics organic spin valve

Dendrimer-induced synthesis of porous organosilica capsules for enzyme encapsulation

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Frontiers of Chemical Science and Engineering 2024年第4期18卷 41-48页

作者： Ziyi Chu Boyu Zhang Zhenhua Wu Jiaxu Zhang Yiran Cheng Xueying Wang Jiafu Shi zhongyi Jiang School of Environmental Science&Engineering Tianjin UniversityTianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering Tianjin 300072China State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China Key Laboratory for Green Chemical Technology of Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China

Organic matter-induced mineralization is a green and versatile method for synthesizing hybrid nanostructured materials,where the material properties are mainly influenced by the species of natural biomolecules,linear synthetic polymer,or small molecules,limiting their diversity.Herein,we adopted dendrimer poly(amidoamine)(PAMAM)as the inducer to synthesize organosilica-PAMAM network(OSPN)capsules for mannose isomerase(MIase)encapsulation based on a hard-templating method.The structure of OSPN capsules can be precisely regulated by adjusting the molecular weight and concentration of PAMAM,thereby demonstrating a substantial impact on the kinetic behavior of the MIase@OSPN system.The MIase@OSPN system was used for catalytic production of mannose from Dfructose.A mannose yield of 22.24% was obtained,which is higher than that of MIase in organosilica network capsules and similar to that of the free enzyme.The overall catalytic efficiency(kcat/Km)of the MIase@OSPN system for the substrate D-fructose was up to 0.556 s^(-1)·mmol^(-1)·L.Meanwhile,the MIase@OSPN system showed excellent stability and recyclability,maintaining more than 50% of the yield even after 12 cycles.

关键词： enzyme immobilization enzyme catalysis organosilica networks capsules sugar biosynthesis

Contrasting response of rice rhizosphere microbiomes to in situ cadmium-contaminated soil remediation

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Soil Ecology Letters 2024年第2期6卷 149-162页

作者： zhongyi Cheng Qinyu Han Yan He Jiachun Shi Laosheng Wu Jianming Xu Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment Institute of Soil and Water Resources and Environmental ScienceCollege of Environmental and Resource SciencesZhejiang UniversityHangzhou310058China Key Laboratory of Environment Remediation and Ecological Health Ministry of EducationHangzhou310058China Department of Environmental Sciences University of CaliforniaRiversideCA92521USA

Changes in soil properties and microbial communities regulated rhizosphere protistan assemblages. • Bacterial community was more sensitive to soil amendments than protists and fungi. • Soil amendments trigger the role of specific protistan taxa Cercozoa on microbial interactions. Understanding the responses of different rhizosphere microbial lineages to soil amendments during in situ remediation of Cd-contaminated soil is of great importance in the assessment of the restoration and crop health. Here, we evaluated the effects of lime (LM), biochar (BC), pig manure (PM), and a commercial Mg-Ca-Si conditioner (CMC) on the rice rhizosphere soil physicochemical properties and community assembly of bacteria, fungi, and protists in a six-year consecutive application of soil amendments field trial. Our results indicated that among the four amendments, the BC and CMC had the best efficiency in increasing soil pH, which were 5.2% and 16.2%, respectively. Despite the differences in soil Cd concentrations is not noticeable, all the soil amendment treatments significantly decreased the proportion of available Cd in total Cd compared to the control. Soil amendments significantly altered the diversity of bacterial community, while they had no effect on fungal and protistan communities. Linear discriminant analysis effect size (LEfSe) showed that the bacteria was more sensitive to soil amendment-induced changes. For protists, treatments with LM and BC changed the groups of protistan consumers, while treatments with PM and CMC significantly increased the relative abundances of protistan phototrophs. Co-occurrence network analysis revealed that soil amendments increased microbial network complexity and triggered the role of protists, especially for the predatory protists Cercozoa, on microbial trophic interactions. Further variation partitioning analysis revealed that edaphic properties, bacterial and fungal communities compositions together explained the 77% of the total variation in protistan community, and the stronger correlat

关键词： cadmium soil amendments soil protist community microbial interactions

The emerging tumor microbe microenvironment: From delineation to multidisciplinary approach-based interventions

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1560-1591页

作者： Yu Fu Jia Li Wenyun Cai Yulan Huang Xinlong Liu zhongyi Ma Zhongjie Tang Xufei Bian Ji Zheng Jiayun Jiang Chong Li Medical Research Institute College of Pharmaceutical SciencesSouthwest UniversityChongqing 400715China Institute of Hepatobiliary Surgery Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Department of Urology Urologic Surgery CenterXinqiao HospitalThird Military Medical University(Army Medical University)Chongqing 400037China

Intratumoral microbiota has become research hotspots,and emerges as a non-negligent new component of tumor microenvironments(TME),due to its powerful infuence on tumor initiation,metas-tasis,immunosurveillance and prognosis despite in low-biomass.The accumulations of microbes,and their related components and metabolites within tumor tissues,endow TME with additional pluralistic features which are distinct from the conventional one.Therefore,it's definitely necessary to comprehen-sively delineate the sophisticated landscapes of tumor microbe microenvironment,as well as their func-tions and related underlying mechanisms.Herein,in this review,we focused on the fields of tumor microbe microenvironment,including the heterogeneity of intratumor microbiota in different types of tu-mors,the controversial roles of intratumoral microbiota,the basic features of tumor microbe microenvi-ronment(i.e.,pathogen-associated molecular patterns(PAMPs),typical microbial metabolites,autophagy,infammation,multi-faceted immunomodulation and chemoresistance),as well as the multi-disciplinary approach-based intervention of tumor microbiome for cancer therapy by applying wild-type or engineered live microbes,microbiota metabolites,antibiotics,synthetic biology and rationally de-signed biomaterials.We hope our work will provide valuable insight to deeply understand the interplay of cancer-immune-microbial,and facilitate the development of microbes-based tumor-specific treatments.

关键词： Tumor microbe microenvironment Microbiota metabolites Immunomodulation Chemoresistance Multidisciplinary approach Genetically engineered microbiota Autophagy Outer membrane vesicles

GmFtsH25 overexpression increases soybean seed yield by enhancing photosynthesis and photosynthates

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Journal of Integrative Plant Biology 2023年第4期65卷 1026-1040页

作者： Li Wang Yuming yang zhongyi yang Wenlong Li Dezhou Hu Huilian Yu Xiao Li Hao Cheng Guizhen Kan Zhijun Che Dan Zhang Hengyou Zhang Hui Wang Fang Huang Deyue Yu National Center for Soybean Improvement National Key Laboratory of Crop Genetics and Germplasm EnhancementJiangsu Collaborative Innovation Center for Modern Crop ProductionNanjing Agricultural UniversityNanjing 210095China Collaborative Innovation Center of Henan Grain Crops College of AgronomyHenan Agricultural UniversityZhengzhou 450046China School of Agriculture Ningxia UniversityYinchuan 750021China Northeast Institute of Geography and Agroecology Key Laboratory of Soybean Molecular Design Breedingthe Chinese Academy of SciencesHarbin 150081China

Increasing plant photosynthetic capacity is a promising approach to boost yields,but it is particularly challenging in C3crops,such as soybean(Glycine max(L.)Merr.).Here,we identified GmFtsH25,encoding a member of the filamentation temperature-sensitive protein H protease family,as a major gene involved in soybean photosynthesis,using linkage mapping and a genome-wide association study.Overexpressing GmFtsH25 resulted in more grana thylakoid stacks in chloroplasts and increased photosynthetic efficiency and starch content,while knocking out GmFtsH25 produced the opposite phenotypes.GmFtsH25 interacted with photosystem I light harvesting complex 2(GmLHCa2),and this interaction may contribute to the observed enhanced photosynthesis.GmFtsH25 overexpression lines had superior yield traits,such as yield per plant,compared to the wild type and knockout lines.Additionally,we identified an elite haplotype of GmFtsH25,generated by natural mutations,which appears to have been selected during soybean domestication.Our study sheds light on the molecular mechanism by which GmFtsH25 modulates photosynthesis and provides a promising strategy for improving the yields of soybean and other crops.

关键词： chloroplast FtsH photosynthesis QTL seed yield soybean