Design of an automated robotic microinjection system for batch injection of zebrafish embryos and larvae

作     者：Zhongyi Guo Nana Ai Wei Ge Qingsong Xu 

作者机构：Department of Electromechanical EngineeringFaculty of Science and TechnologyUniversity of MacaoMacaoChina Department of Biomedical Sciences and Centre of ReproductionDevelopment and Aging(CRDA)Faculty of Health SciencesUniversity of MacaoMacaoChina 

出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)

年 卷 期：2024年第10卷第1期

页      面：89-103页

核心收录：

学科分类：080202[工学-机械电子工程] 08[工学] 0804[工学-仪器科学与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0802[工学-机械工程] 

基　　金：supported in part by the National Natural Science Foundation of China under Grant 52175556 the Macao Science and Technology Development Fund under Grants 0102/2022/A2 and 0004/2022/AKP the University of Macao under Grants MYRG2022-00068-FST,MYRG2022-00219-FHS and MYRG-CRG2022-00004-FST-ICI the Guangdong Basic and Applied Basic Research Foundation under Grant 2023A1515011178 

主　　题：zebra automated hardware 

摘      要：This paper presents the design of a vision-based automated robotic microinjection system for batch injection of both zebrafish embryos and larvae.A novel visual recognition algorithm based on an automatic threshold and excessive dilatation is introduced to accurately identify the center of zebrafish embryos and larval yolks.A corresponding software system is developed using the producer-consumer model as the framework structure,and a friendly user interface is designed to allow operators to choose from a range of desired functions according to their different *** addition,a novel microstructural agarose device is designed and fabricated to simultaneously immobilize mixed batches of embryos and ***,a prototype microinjection system is fabricated by integrating hardware devices with visual *** experimental study is conducted to verify the performance of the robotic microinjection *** results show that the reported system can accurately identify zebrafish embryos and larvae and efficiently complete batch microinjection tasks of the mixtures with an injection success rate of 92.05%in 13.88 s per *** with manual and existing microinjection systems,the proposed system demonstrates the merits of versatility,excellent efficiency,high success rate,high survival rate,and sufficient stability.