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Electrophoretic Determination of Aqueous and Serum Neuron-specific Enolase in the Diagnosis of Retinoblastoma

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眼科学报 1997年第1期13卷 12-16页

作者： zhongyao wu, huasheng yang, Suhua Pan, Zhicong Chenzhongshan ophthalmic center, sun yat-sen university of medical sciences , guangzhou 510060 , china zhongshan ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China.

Purpose: Neuron-specific enolase (NSE) of containing γ-enolase is considered valuable in the diagnosis of tumours of neuroectodermal *** : We used rapid electrophoretic method on cellulose acetate plate to determine the pattern of enolase isoenzymes in 21 aqueous humor and 23 serum specimens from retinoblastoma (Rb) and 21 aqueous and 25 serum specimens from 25 control cases to evaluate NSE in the diagnosis of Rb. The assay allowed assessment of all three major isoenzymes (aa,aγ and γγ),and NSE relative activity and its percentage in the total relative activity of the three enolase isoenzymes were assessed by means of ***: Aqueous from all patients with Rb contained aa,ar and rr isoenzymes and presented strong postitive, the positive rate of NSE being 100% and its relative activity accounting for 45 ± 9% of the total relative activity of the 3 enolase isoenzymes; No enolase was detectable in control aqueous with cataract, glaucoma and Coats's diseases (4 cases),but in two

关键词：眼免疫电泳水状体血清成视网膜细胞瘤神经元性烯醇酶

A Clinical Analysis of Idiopathic Orbital Inflammatory Pseudotumor

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眼科学报 2000年第3期16卷 208-213页

作者： Jianhua Yan, zhongyao wu, Yongping Lizhongshan ophthalmic center, sun yat-sen university of medical sciences. guangzhou 510060 china zhongshan ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

Purpose: To observe the clinical findings and response to treatment in patients with a diagnosis of idiopathic orbital inflammatory ***: 209 idiopathic orbital inflammatory pseudotumor cases seen between Jan 1, 1978 and Dec 31, 1999 in our hospital were evaluated retrospectively. Results: Of the 209 cases, 118 were male and 91 were female; there were 90 in the right eye, 81 left eye and 38 both eyes. Patients age ranged from 4 to 80 years (mean 44.4). Proptosis (66%), palpable mass (65%), swollen eyelid (55%), increased orbital pressure (55%) and motility restriction (48%) were the five most common presenting signs. According to radiologic and surgical findings, focal mass within orbit was the most frequent subtype (43% ), followed by lacrimal inflammatory pseudotumor (32%), diffuse orbital inflammation (10%), myositis (8%) . Perineuritis (2%), periscleritis(2%), acute inflammation (2%) and eyelid pseudotumor (1%) were rare clinical findings. The response to treatment (with a mean

关键词：炎性假瘤眼眶肿瘤自发性临床分析

A Model for Blindness Prevention

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眼科学报 1997年第3期13卷 162-163,147页

作者： Jingjing Xu, Wenshu Mao, Shaozhen Li, Lezheng wu, zhongyao wu Siping Zhu, Qiang Yu, Mingguang Hezhongshan ophthalmic center, sun yat-sen university of medical sciences, guangzhou 510060, china zhongshan ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

Objective :To introduce the work of the prevention and treatment of blindness of zhongshan ophthalmic center, to find out the effective model of blindness prevention and treatment in ***; 1. To provide high quality clinical service to rural people. 2. To conduct epidemio-logical survey *** train local ophthalmic professionals *** promote international ***:40 000 outpatients cases, 4 500 cataract surgeries have been accomplished. Thousands cataract blind have been rehabilitated. 9 papers concerning epidemiological survey have been published. After the international training courses, 50 local ophthalmic workers have been trained and 4 cataract surgery rehabilitation centers have been founded. Conclusion : An effective model for blindness prevention and treatment should be based on clinical service, population based epidemiological survey and local ophthalmic professionals training. International cooperation is also an important promoter. Eye Science 1997; 13 : 162 -163.

关键词：失明预防方法白内障

Retinoma and Phthisis Bulbi of Retinoblastoma *** and Genetic Analysis

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眼科学报 1992年第3期8卷 117-121页

作者： Qingjiong Zhang Youzhao Chen zhongyao wu Qiaoyun Ma Ruiping Zeng Xiangming Guo Danyang Li National Ophthalmological Laboratories,Ministry of Public Health,china zhongshan ophthalmic center,sun yat-sen university of medical sciences,guangzhou 510060,china National Ophthalmological Laboratories Ministry of Public Health China.

Retinoma and phthisis bulbi of retinoblastoma are rare entitiesfound in retinoblastoma patients and their *** cases of phthisisbulbi of retinoblastoma and 9 cases of retinoma were identified from 1966 to1991 in our *** clinic data show that retinoma and phthisis bulbi areclosely related to the retinoblastoma *** should be carried outas soon as possible without hesitation for the phthisis of eyes with *** counseling and frequent observation should be p...

关键词： Retinoma Retinoblastoma phthisis bulbi spontaneous regression

Exon-by-exon Screening for RB Germline Mutations Using Heteroduplex-SSCP Analysis

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眼科学报 1997年第1期13卷 5-11页

作者： Qingjiong Zhang, Kensei Minoda, Ruiping Zeng, zhongyao wu Xueshan Xiao, Shiqiang Li, Fengshen Zhangzhongshan ophthalmic center Department of Medica Geneticssun yat-sen university of medical sciences , guangzhou 510060 , chinaDepartment of Ophthalmology, Teikyo university Ichihara Hospital, Ichihara , Chiba 299 - 01, Japan. zhongshan ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China.

Objective: To disclose the nature of RBI germline mutations in Chinese and to develop a practical and effective way for mutational screening. Methods: Leukocyte DNA was prepared from 8 Chinese patients with hereditary retinoblastoma. PCR combined with nonisotopic heteroduplex-SSCP analysis was used to screen leukocyte DNA for RBI germline mutations, exon-by-exon, without the use of restriction endonuclease digestion. The mutations were finally identified by sequencing. In order to testify the effectiveness of this method, the same method was used to detect other 17 samples which have been previously analyzed by other methods. Results: Heterozygous germline mutations were detected in the leukocyte DNA of 6 out of 8 Chinese patients;G del/codon 46,T del/codon 131 ,CAGAA del/condon 257 -258, GCAgta→GCAgca/donor of exon 16, C@T/codon 661, and C→T/codon 787. Heteroduplex-SSCP analysis may detect RBI germline mutations in 68% (177 25) unselected patients, which is more effective than SSCP(56%) or

关键词：成视网膜细胞瘤突变 SSCP 遗传因素非遗传因素

Graves'眼病患者血清可溶性白细胞介素-2受体测定的临床意义

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眼科学报 1999年第2期15卷 78-80页

作者：杨华胜吴中耀黄祥坤谢楚芳杨培增中山医科大学中山眼科中心广州510060

目的:探讨血清中可溶性白细胞介素-2受体(sIL-2R)水平与Graves’眼病的临床表现严重程度和治疗效果的关系。方法:用双抗体夹心ELISA法检测甲状腺功能正常Graves’眼病患者41例和正常对照组49例血清sIL-2R。结果:Graves’眼病患者未用糖... 详细信息

目的:探讨血清中可溶性白细胞介素-2受体(sIL-2R)水平与Graves’眼病的临床表现严重程度和治疗效果的关系。方法:用双抗体夹心ELISA法检测甲状腺功能正常Graves’眼病患者41例和正常对照组49例血清sIL-2R。结果:Graves’眼病患者未用糖皮质激素治疗前血清sIL-2R水平明显高于正常对照组(P<0.001);甲亢型Graves’眼病组与眼型Graves’病组其sIL-2R水平无显著性差异;其治疗前血清SIL-2R在糖皮质激素治疗显效的病例高于无明显治疗效果的病例(P<0.001);sIL-2R的水平与按NOSPECS分级的疾病严重程度无相关。12例患者治疗3个月后复查其sIL-2R水平较治疗前明显降低(P<0.001)。结论:Graves’眼病血清sIL-2R升高可能与眼眶局部淋巴细胞活化有关,可作为评价Graves’眼病的疾病活动性和糖皮质激素治疗效果的参考指标。眼科学报1999;15:78—80。

关键词： Graves'眼病白细胞介素-2 受体突眼性甲状腺肿

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牵牛花综合征B超检查所见

眼科学报 1998年第2期14卷 103-104页

作者：吴京红邹丽娟莫燕琼吴中耀中山医科大学中山眼科中心

病例1:患儿,男,4岁。发现右眼视物不见1月,当地医院临床检查、B超、CT诊断为“右眼视神经占位性病变(视神经胶质瘤)”。患儿遂于1997年1月到本院就诊。眼部检查:右眼视力无光感。外观较对侧细小,眼前段无异常,眼底检查发现视乳头边界不... 详细信息

病例1:患儿,男,4岁。发现右眼视物不见1月,当地医院临床检查、B超、CT诊断为“右眼视神经占位性病变(视神经胶质瘤)”。患儿遂于1997年1月到本院就诊。眼部检查:右眼视力无光感。外观较对侧细小,眼前段无异常,眼底检查发现视乳头边界不清,视乳头周围可见大片灰白色隆起物,血管扭曲,周边视网膜平伏。左眼未见异常。B超检查:右眼轴 20.5mm,左眼轴 22.8mm,右眼视乳头向后凹陷,凹陷后方相当于正常视神经部位见一圆形无回声区,大小约 8.97mm×9.88mm。

关键词：牵牛花综合征 B超诊断

The Experiment Research of Subconjunctival Injections with Etoposide for the Treatment of Retinoblastoma Mice

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Eye Science 2005年第3期21卷 169-174页

作者： Yuxiang Mao zhongyao wu huasheng yang Jianliang Zheng Jianfeng He Wei Li zhongshan ophthalmic center Sun Yat-sen University Guangzhou 510060 China

Purpose: To evaluate the efficacy of subconjunctival injections of Etoposide for retinoblastoma(RB) ***: Twenty-five RB mice were divided into 5 groups for the study(G0～G4). GO for control group received 0.9% sodium chloride injection subconjunctival. G1: 5 RB mice for systemic delivery received 6 systemic administration of Etoposide 150 μg and Cyclosporin A (CSA)1.0mg at 3 days intervals, respectively. G2～G4:5 RB mice in each group received 6 subconjunctival delivery of Etoposide in 50、75、150μg at 3 days intervals, *** observe the changes of the volume of RB in anterior chamber and the part of conjunctiva for injections of chemotherapeutic drugs. All eyes and conjunctiva and scleras of the part for drugs injections were obtained at 6 weeks after experiment for histopathologic examination. The findings under the light microscope were analysed with software to measure the areas of RB in anterior chambers and the tumor inhibition ***:The volume of RB in anterior chambers for G1 and G2 increase quickly and none of the mice exhibited tumor control. The RB volume increase slowly in G3 and exhibit tumor control in G4. The areas of tumors in G0～G4 are 0.710,0.659,0.634,0.515,0.235 mm2, respectively. The rate for tumor inhibition in G0～G4 are 0.0%,7.18%, 10.70% ,27.43% ,66.90%, respectively. There are statistical significant difference between the G1 and G4 for the volumes of tumors and the rate of tumor *** pathologic examination of subconjunctiva and sclera for the injective sites showed no pathologic ***: There are significant effects for the method of subconjunctival delivery of Etoposide in RB control, demonstrating a positive dose-related relationship. Subconjunctival injection of Etoposide reached better efficacy in controlling and inhibiting RB cells than systemic delivery of Etoposide for the same dose. The pathologic results showed the subconjunctival delivery of etoposide were safety for conjunctiva and scle

关键词：医学实验注射剂眼癌小鼠动物实验治疗方法

血清烯醇化酶定量检测对视网膜母细胞瘤诊断和监测的应用价值

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中华眼科杂志 1998年第2期34卷 117-120页

作者：吴中耀毛羽翔杨华胜潘苏华陈智聪中山医科大学中山眼科中心

目的探讨血清烯醇化酶，尤其是神经元特异性烯醇化酶（ｎｅｕｒｏｎ－ｓｐｅｃｉｆｉｃｅｎｏｌａｓｅ，ＮＳＥ）含量测定对视网膜母细胞瘤（ｒｅｔｉｎｏｂｌａｓｔｏｍａ，Ｒｂ）诊断和病情监测的价值。方法应用醋酸纤维薄膜快速电泳... 详细信息

目的探讨血清烯醇化酶，尤其是神经元特异性烯醇化酶（ｎｅｕｒｏｎ－ｓｐｅｃｉｆｉｃｅｎｏｌａｓｅ，ＮＳＥ）含量测定对视网膜母细胞瘤（ｒｅｔｉｎｏｂｌａｓｔｏｍａ，Ｒｂ）诊断和病情监测的价值。方法应用醋酸纤维薄膜快速电泳＋荧光分光光度法，对３５例经病理检查确诊的Ｒｂ患者，分别于眼球摘除时，术后３、６、１２和≥１８个月后及３１例对照组进行血清烯醇化酶总量和ＮＳＥ含量定量检测，观察比较血清烯醇化酶总量和ＮＳＥ含量的变化。结果Ｒｂ患者血清烯醇化酶总量为２０７．０±６５．９Ｕ／Ｌ，ＮＳＥ含量为９８．４±４０．３Ｕ／Ｌ，与对照组血清测定值（分别为１４０．７±４６．０Ｕ／Ｌ和４１．０±１０．９Ｕ／Ｌ）比较明显升高，两组间差异有显著性（Ｐ＜０．００１）。８２．６％Ｒｂ患者血清ＮＳＥ含量明显高于对照组上限水平。Ｒｂ患儿术后６、１２和≥１８个月血清烯醇化酶总量和ＮＳＥ含量逐渐下降（ＮＳＥ含量下降较缓慢），与治疗前的测定值相比差异有显著性；至≥１８个月后血清ＮＳＥ含量和烯醇化酶总量降至对照组测定值水平。结论血清烯醇化酶尤其ＮＳＥ含量测定对Ｒｂ患者有一定的辅助诊断价值。

关键词：血清烯醇化酶定量检测视网膜母细胞瘤 NSE 肿瘤磷酸丙酮酸水合酶