Electrophoretic Determination of Aqueous and Serum Neuron-specific Enolase in the Diagnosis of Retinoblastoma

作     者：Zhongyao Wu, Huasheng Yang, Suhua Pan, Zhicong ChenZhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences , Guangzhou 510060 , China 

作者机构：Zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China. 

出 版 物：《眼科学报》 (Eye Science)

年 卷 期：1997年第13卷第1期

页      面：12-16页

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 100214[医学-肿瘤学] 10[医学] 

基　　金：2020B1515020038 

主　　题：眼 免疫电泳 水状体 血清 成视网膜细胞瘤 神经元性烯醇酶 

摘      要：Purpose: Neuron-specific enolase (NSE) of containing γ-enolase is considered valuable in the diagnosis of tumours of neuroectodermal *** : We used rapid electrophoretic method on cellulose acetate plate to determine the pattern of enolase isoenzymes in 21 aqueous humor and 23 serum specimens from retinoblastoma (Rb) and 21 aqueous and 25 serum specimens from 25 control cases to evaluate NSE in the diagnosis of Rb. The assay allowed assessment of all three major isoenzymes (aa,aγ and γγ),and NSE relative activity and its percentage in the total relative activity of the three enolase isoenzymes were assessed by means of ***: Aqueous from all patients with Rb contained aa,ar and rr isoenzymes and presented strong postitive, the positive rate of NSE being 100% and its relative activity accounting for 45 ± 9% of the total relative activity of the 3 enolase isoenzymes; No enolase was detectable in control aqueous with cataract, glaucoma and Coats s diseases (4 cases),but in two