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Inhibitory effects of prostaglandin E₁ on activation of hepatic stellate cells in rabbits with schistosomiasis

Hepatobiliary & Pancreatic Diseases International 2007年第2期6卷 176-181页

作者： WeiLong Zou Zhen Yang YunJin Zang DongJian Li ZhiPeng Liang zhongyang shen Institute of Transplantation General Hospital of the Chinese People's Armed Police Forces Beijing China. wlzouah@***

BACKGROUND: Liver fibrosis is the result of an imbalance between synthesis and degradation of extracellular matrix proteins of the liver. At the cellular and molecular levels, this progressive process is mainly characterized by activation of hepatic stellate cells (HSCs). Schistosoma japonica is one of the most prevalent causes of liver fibrosis in China. It is characterized by hepatocyte damage, inflammation, and chronic parasite egg-induced granuloma formation leading to fibrosis. This study aimed to investigate the inhibitory effects of prostaglandin E1 (PGE1) on activation of HSCs and the alteration of type Ⅰ and Ⅲ collagen in rabbits with schistosomiasis. The study may promote the clinical application of praziquantel and PGE1 as a combined therapy to reverse hepatic fibrosis caused by schistosomiasis. METHODS: Rabbits were percutaneously infected with cercaria of S. japonicum. Seven rabbits were subjected to intravenous injections of PGE1 (2.5 μg/kg daily) from days 60 to 120 after infection. The ultrastructural changes in activated HSCs were observed under transmission electron microscopy. The expression of α-smooth muscle actin (α-SMA) was detected by immunohistochemistry. Fibril-forming collagens were detected by picrosirius staining. RESULTS: Activation of HSCs was a characteristic alteration in schistosome-induced hepatic fibrosis. The expression of contraction-related α-SMA and thecontent of collagens were increased. Exogenous PGE1 markedly inhibited the activation of HSCs and reduced the expression of α-SMA around the hepatic sinusoids (P<0.01). The contents of type Ⅰ and Ⅲ collagens were significantly attenuated. The ratio of staining area to the whole field (10×3.3) under a polarized light microscope in the untreated and treated groups was 37.25±9.71 vs. 13.38±4.24 (P<0.01) and 9.66±3.52 vs. 6.23±1.81 (P<0.05), respectively. CONCLUSIONS: Activation of HSCs may play a key role in the progress of schistosome-induced hepatic fibrosis. PGE1 effectively protects rabbit liver from fibrosis, at le

关键词： hepatic fibrosis schistosome hepatic stellate cells prostaglandin E1 fibril-forming collagen

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Micro-nanofiber composite biomimetic conduits promote long-gap peripheral nerve regeneration in canine models

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Bioactive Materials 2023年第12期30卷 98-115页

作者： Xianhao Dong Yueyue Yang Zheheng Bao Adam C.Midgley Feiyi Li Shuxin Dai Zhuangzhuang Yang Jin Wang Lihua Liu Wenlei Li Yayuan Zheng Siyang Liu Yang Liu Weijian Yu Jun Liu Meng Fan Meifeng Zhu zhongyang shen Gu Xiaosong Deling Kong State Key Laboratory of Medicinal Chemical Biology The Key Laboratory of Bioactive MaterialsMinistry of EducationCollege of Life ScienceNankai UniversityTianjinChina Department of Orthopaedics Tianjin First Central HospitalSchool of MedicineNankai UniversityTianjinChina Department of Radiology Tianjin First Central HospitalTianjin Medical Imaging InstituteSchool of MedicineNankai UniversityTianjinChina Institute of Transplantation Medicine NHC Key Laboratory for Critical Care MedicineTianjin First Central HospitalSchool of MedicineNankai UniversityTianjinChina Haihe Laboratory of Sustainable Chemical Transformations Keyan West RoadTianjin300192China Jiangsu Key Laboratory of Neuroregeneration Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury RepairCo-innovation Center ofNeuroregenerationNMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology ProductsNantong UniversityNantongChina Outpatient Department Tianjin First Central HospitalSchool of MedicineNankai UniversityTianjinChina Clinical School/College of Orthopedics Tianjin Medical UniversityTianjinChina Department of Joint Tianjin HospitalTianjinChina

Peripheral nerve injuries may result in severe long-gap interruptions that are challenging to repair.Autografting is the gold standard surgical approach for repairing long-gap nerve injuries but can result in prominent donor-site complications.Instead,imitating the native neural microarchitecture using synthetic conduits is expected to offer an alternative strategy for improving nerve regeneration.Here,we designed nerve conduits composed of high-resolution anisotropic microfiber grid-cordes with randomly organized nanofiber sheaths to interrogate the positive effects of these biomimetic structures on peripheral nerve regeneration.Anisotropic microfiber-grids demonstrated the capacity to directionally guide Schwann cells and neurites.Nanofiber sheaths conveyed adequate elasticity and permeability,whilst exhibiting a barrier function against the infiltration of fibroblasts.We then used the composite nerve conduits bridge 30-mm long sciatic nerve defects in canine models.At 12 months post-implant,the morphometric and histological recovery,gait recovery,electrophysiological function,and degree of muscle atrophy were assessed.The newly regenerated nerve tissue that formed within the composite nerve conduits showed restored neurological functions that were superior compared to sheaths-only scaffolds and Neurolac nerve conduit controls.Our findings demonstrate the feasibility of using synthetic biophysical cues to effectively bridge long-gap peripheral nerve injuries and indicates the promising clinical application prospects of biomimetic composite nerve conduits.

关键词： composite fiber injuries

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Treatment of early duodenal fistula after orthotopic liver transplantation: a case report

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Hepatobiliary & Pancreatic Diseases International 2003年第1期2卷 155-157页

作者： Yun Lu LiQun Wu BingYuan Zhang Bin Zhang JingYu Cao zhongyang shen Department of Hepatobiliary Vascular Surgery Affiliated Hospital of Medical College Qingdao University Qingdao 266003 China. cloudylucn@***

Gastrointestinal fistula as a serious complicationcould lead to imbalance of nutrition or death.Duodenal fistula after orthotopic liver transplanta-tion is rare and its treatment is complicated. OnApril 28, 2000, we performed orthotopic livertransplantation for a patient at our hospital. Eightdays after operation duodenal fistula developed,but cured after 13-day treatment.Case reportA 46-year-old woman weighing 50 kg was admittedto our hospital because of emaciation and yellowishbody skin for over half a year. In 1994, she hadundergone cholecystectomy and choledochoduode-nostomy for cholangitis elsewhere. One month be-

关键词： of is in as case a case report Treatment of early duodenal fistula after orthotopic liver transplantation

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IRF-1在肝脏疾病调控中的关键作用：进展与展望（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第6期25卷 451-471页

作者：陈涛李世朋邓德文张玮晔张建军沈中阳天津医科大学第一中心临床学院河南省人民医院肝胆外科天津市第一中心医院器官移植重点实验室天津市第一中心医院器官移植科南开大学移植医学研究院

干扰素调节因子1(IRF-1)是干扰素调节因子家族的重要一员，其主要功能是能够与靶基因上的干扰素刺激反应元件（ISRE）结合，进而在干扰素诱导的信号通路中发挥重要作用。IRF-1作为一种重要的转录因子，在细胞周期调控、细胞死亡信号转... 详细信息

干扰素调节因子1(IRF-1)是干扰素调节因子家族的重要一员，其主要功能是能够与靶基因上的干扰素刺激反应元件（ISRE）结合，进而在干扰素诱导的信号通路中发挥重要作用。IRF-1作为一种重要的转录因子，在细胞周期调控、细胞死亡信号转导、增强免疫监视等方面具有重要作用。当前研究表明，IRF-1在多种肝脏疾病的发生和发展中发挥至关重要的作用，包括抑制肝炎病的复制、缓解肝纤维化的进展和加重肝缺血再灌注损伤（HIRI）等。IRF-1还作为一种重要的肿瘤抑制因子，在临床上可作为预测肝癌预后和复发的潜在指标。此外，最新的研究还揭示了IRF-1在介导其他肝脏疾病中的潜在作用，如预防酒精性/非酒精性脂肪性肝病，抑制胆管细胞癌的生物学进展，预防细胞排斥反应等。虽然当前的研究极少涉及IRF-1的临床转化领域，但几种化合物和药物似乎可以通过激活IRF-1信号通路，在动物体特定肝脏疾病模型中发挥保护功能。在本文中，我们将综述既往文献以帮助解读IRF-1在肝脏疾病治疗中的研究进展，并提供未来转化医学应用的理论基础。

关键词：干扰素调节因子1(IRF-1) 肝炎病毒肝纤维化肝缺血再灌注损伤(HIRI) 肝癌

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Liver retransplantation:report of 80 cases and review of literature

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Hepatobiliary & Pancreatic Diseases International 2006年第2期5卷 180-184页

作者： zhongyang shen ZhiJun Zhu YongLin Deng Hong Zheng Cheng Pan YaMing Zhang Rui Shi WenTao Jiang JianJun Zhang Orient Organ Transplant Center First Central Hospital Tianjin 300192 China. shenzy@***.

BACKGROUND: Because the orthotopic liver transplantation (OLT) was performed widely in recent 5 years throughout China, the proportion of recipients whose graft function deteriorated to be retransplantation candidates increased gradually. This study was undertaken to analyze clinical experience of orthotopic liver retransplantation (re-OLT) at our center. METHODS: The medical records of 80 patients who had undergone liver retransplantation at our center from January 1999 to July 2005 were analyzed retrospectively, including indications and timing of retransplantation, surgical techniques, and the causes of death. RESULTS: The commonest cause leading to hepatic graft loss and subsequent retransplantation was biliary complications in 36 patients (45%). The patients underwent retransplantation more than 30 days after their primary transplant recovered better than those who underwent retransplantation within 8-30 days after primary transplantation (peri-operative mortality 19.6% versus 70%). Sepsis (12 of 22 patients, 54.5%) and multiple organ failure (4 of 22 patients, 18.2%) were leading causes of re-OLT recipient deaths. CONCLUSIONS: Proper indications and optimal operative time, surgical procedures, perioperative monitoring and appropriate postoperative treatment contribute to the improvement of the survival rate of patients after liver retransplantation.

关键词： liver retransplantation

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谷朊粉对大豆分离蛋白膜理化性质的影响

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中国粮油学报 2024年第5期39卷 79-85页

作者：江睿钊石林凡任中阳杨燊张玉苍翁武银集美大学海洋食品与生物工程学院厦门361021 厦门市海洋功能食品重点实验室厦门361021

为利用枧水处理的谷朊粉(WG)改良大豆分离蛋白(SPI)膜性质,考察了m_(WG)∶m_(SPI)分别为0∶10、1∶9、2∶8、3∶7、4∶6时对复合膜理化性质的影响,测定膜的微观结构、蛋白结构、机械性能等理化性质。扫描电子显微镜结果显示添加的WG主... 详细信息

为利用枧水处理的谷朊粉(WG)改良大豆分离蛋白(SPI)膜性质,考察了m_(WG)∶m_(SPI)分别为0∶10、1∶9、2∶8、3∶7、4∶6时对复合膜理化性质的影响,测定膜的微观结构、蛋白结构、机械性能等理化性质。扫描电子显微镜结果显示添加的WG主要聚集在复合膜下表面,当m_(WG)∶m_(SPI)超过2∶8时膜的下表面网络结构会遭到破坏。根据傅里叶变换红外光谱和十二烷基硫酸钠-聚丙烯酰氨凝胶电泳的结果,发现WG会阻碍SPI分子之间的交联。SPI膜的抗拉伸强度和断裂伸长率分别为6.60 MPa和54.91%,伴随WG的添加抗拉伸强度逐渐下降而断裂伸长率逐渐上升。复合膜的水蒸气阻隔能力和上表面接触角随着WG比例的增加而增大。当m_(WG)∶m_(SPI)增加到2∶8时,WG/SPI复合膜的b*值达到了18.72的高值。差示扫描量热法的结果表明,在m_(WG)∶m_(SPI)为1∶9时,复合膜的热稳定性最高。研究结果表明适当添加枧水预处理的WG能提高SPI膜的部分物理性质,这将为SPI膜理化性质改良提供新的途径。

关键词：大豆分离蛋白谷朊粉枧水复合膜水蒸气透过率

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器官移植适应证:横观与纵览

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实用器官移植电子杂志 2024年第1期12卷 1-2页

作者：沈中阳薛武军天津市器官移植研究中心南开大学移植医学研究院天津300192 西安交通大学第一附属医院肾脏病医院肾移植科西安交通大学器官移植研究所陕西西安710061

适应证是熟知未必详知、常用未必用准的医学概念,是通用名词——指征(indication)在医药领域的专属化用语,其融汇指示与征象等基本词义,用以指向性表述某一临床措施的具体适用情形、证据或理由。适应证具有指示性与适当性双重基本内涵,... 详细信息

适应证是熟知未必详知、常用未必用准的医学概念,是通用名词——指征(indication)在医药领域的专属化用语,其融汇指示与征象等基本词义,用以指向性表述某一临床措施的具体适用情形、证据或理由。适应证具有指示性与适当性双重基本内涵,同时具有复杂性、时代性两项基本特征。适应证通常是其所属时代背景下科技进步程度和医药发展水平的生动写照,追溯适应证变迁历程可发掘医学进步的内在动力与演进规律。

关键词：器官移植基本词义医学进步临床措施科技进步指示性适用情形变迁历程

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肝移植适应证的变迁

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实用器官移植电子杂志 2024年第1期12卷 48-54页

作者：李俊杰杨健郑虹沈中阳天津市第一中心医院肝脏移植科天津300000

自1963年美国医生Starzl施行世界首例人体原位肝移植以来,临床肝移植历经60年的发展,取得了巨大进步,各种类型的终末期肝病均已纳作肝移植适应证^([1])。适应证通常是指适合于采用某一治疗措施的疾病或情况,而伴随时代与科技的进步,适... 详细信息

自1963年美国医生Starzl施行世界首例人体原位肝移植以来,临床肝移植历经60年的发展,取得了巨大进步,各种类型的终末期肝病均已纳作肝移植适应证^([1])。适应证通常是指适合于采用某一治疗措施的疾病或情况,而伴随时代与科技的进步,适应证常经过悄然变迁而促成历史性跨越。回眸肝移植发展历程,同样可见证其适应证变迁的细节与成果。

关键词：终末期肝病肝移植适应证临床肝移植治疗措施历史性跨越发展历程

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pH对大黄鱼肌原纤维蛋白结构性质及乳化性的影响

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水产学报 2024年第3期48卷 164-173页

作者：任中阳崔雅清陈玉峰石林凡郝更新杨燊邱绪建刘淑集翁武银集美大学海洋食品与生物工程学院集美大学厦门市海洋功能食品重点实验室浙江工业大学浙江省深蓝渔业资源高效开发利用重点实验室浙江工业大学食品科学与工程学院福建省水产研究所

为深入探究大黄鱼肌原纤维蛋白(MP)的乳化性，以满足大黄鱼蛋白质高值化开发利用的需求。实验探究了不同pH对大黄鱼MP结构性质及其乳化性的影响。采用pH分别为2、4、6、8、10、12的低盐磷酸缓冲液处理大黄鱼MP，通过SDS-PAGE分析大黄鱼M... 详细信息

为深入探究大黄鱼肌原纤维蛋白(MP)的乳化性，以满足大黄鱼蛋白质高值化开发利用的需求。实验探究了不同pH对大黄鱼MP结构性质及其乳化性的影响。采用pH分别为2、4、6、8、10、12的低盐磷酸缓冲液处理大黄鱼MP，通过SDS-PAGE分析大黄鱼MP降解情况，利用接触角测量仪和荧光分光光度计考察其疏水性变化。将不同pH下大黄鱼MP溶液与大豆油以体积比为1∶1的比例经高速均质制备乳液，并对其乳滴电位、粒径以及乳液的乳析指数进行测定，综合分析在不同pH下乳液的稳定性。SDS-PAGE电泳结果表明，在pH 4时，大黄鱼MP条带颜色较浅，在pH 6～12时，肌球蛋白重链条带基本消失，肌动蛋白条带颜色逐渐加深，且在浓缩胶顶部出现了高分子聚集物。对接触角和荧光光谱的综合分析表明，大黄鱼MP疏水性随pH增加而增加。对不同pH下MP乳液特性分析显示，在pH 8时，乳滴带负电且Zeta电位绝对值大[(49.63±1.52) mV]、粒径较小、乳液稳定性较好，而pH 12条件下容易出现破乳，不利于乳液的稳定。在pH 8时，大黄鱼MP结构更有利于提升乳液稳定性。研究表明，在弱碱性条件下更有利于大黄鱼MP形成稳定的乳液体系。pH对大黄鱼MP结构性质和乳化性的影响探究，有望为大黄鱼MP在食品工业中的开发利用提供理论依据。

关键词：大黄鱼肌原纤维蛋白 pH 疏水性乳化性

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Innate immune imprints in SARS-CoV-2 Omicron variant infection convalescents

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Signal Transduction and Targeted Therapy 2022年第12期7卷 4392-4404页

作者： Zhiqing Li Xiaosu Chen Junyan Dan Tianju Hu Ye Hu Shuxun Liu Yangyang Chai Yansong Shi Jian Wu Hailai Ni Jiaqi Zhu Yanfeng Wu Nan Li Yizhi Yu Zhongfang Wang Jincun Zhao Nanshan Zhong Xianwen Ren zhongyang shen Xuetao Cao National Key Laboratory of Medical Immunology Institute of ImmunologyNaval Medical UniversityShanghai200433China Frontier Research Center for Cell Response Institute of ImmunologyCollege of Life SciencesNankai UniversityTianjin300071China Department of Immunology Institute of Basic Medical ResearchPeking Union Medical CollegeChinese Academy of Medical SciencesBeijing100005China The Health Care Department Shanghai Changhai HospitalShanghai200433China Department of Cardiology Shanghai Changhai HospitalShanghai200433China Guangzhou Laboratory Guangzhou510300China Changping Laboratory Beijing102206China Organ Transplant Center Tianjin First Central HospitalNankai UniversityTianjin300192China

SARS-CoV-2 Omicron variant infection generally gives rise to asymptomatic to moderate COVID-19 in vaccinated people.The immune cells can be reprogrammed or“imprinted”by vaccination and infections to generate protective immunity against subsequent challenges.Considering the immune imprint in Omicron infection is unclear,here we delineate the innate immune landscape of human Omicron infection via single-cell RNA sequencing,surface proteome profiling,and plasma cytokine quantification.We found that monocyte responses predominated in immune imprints of Omicron convalescents,with IL-1β-associated and interferon(IFN)-responsive signatures with mild and moderate symptoms,respectively.Low-density neutrophils increased and exhibited IL-1β-associated and IFN-responsive signatures similarly.Mild convalescents had increased blood IL-1β,CCL4,IL-9 levels and PI3+neutrophils,indicating a bias to IL-1βresponsiveness,while moderate convalescents had increased blood CXCL10 and IFN-responsive monocytes,suggesting durative IFN responses.Therefore,IL-1β-or IFN-responsiveness of myeloid cells may indicate the disease severity of Omicron infection and mediate post-COVID conditions.

关键词： infection protective immunity

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