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Fibroblast-localized lncRNA CFIRL promotes cardiac fibrosis and dysfunction in dilated cardiomyopathy

Science China(Life Sciences) 2024年第6期67卷 1155-1169页

作者： Shuai Yuan Xudong Zhang Jiabing Zhan Rong Xie Jiahui Fan Beibei Dai Yanru Zhao zhongwei yin Qian Liu Dao Wen Wang Huaping Li Chen Chen Division of Cardiology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Hubei Key Laboratory of Genetics and Molecular Mechanisms of Cardiological Disorders Wuhan 430030China Department of Forensic Medicine Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

CFIRL is a long noncoding RNA(lnc RNA),we previously identified as the most significantly upregulated lnc RNA in the failing hearts of patients with dilated cardiomyopathy(DCM).In this study,we determined the function of CFIRL and its role in DCM.Real-time polymerase chain reaction and in situ hybridization assays revealed that CFIRL was primarily localized in the nucleus of cardiac fibroblasts and robustly increased in failing hearts.Global knockdown or fibroblast-specific knockout of CFIRL attenuated transverse aortic constriction(TAC)-induced cardiac dysfunction and fibrosis in vivo.Overexpression of CFIRL in vitro promoted fibroblast proliferation and aggravated angiotensin II-induced differentiation to myofibroblasts.CFIRL knockdown attenuated these effects.Mechanistically,RNA pull-down assay and gene expression profiling revealed that CFIRL recruited ENO1,a newly identified noncanonical transcriptional factor,to activate IL-6transcription.IL-6 exerted a paracrine effect on cardiomyocytes to promote cardiac hypertrophy,which can be prevented by CFIRL knockdown.These findings uncover the critical role of CFIRL,a fibroblast-associated lnc RNA,in heart failure by facilitating crosstalk between fibroblasts and cardiomyocytes.CFIRL knockdown might be a potent strategy to prevent cardiac remodeling in heart failure,particularly in DCM.

关键词： lncRNA fibroblast fibrosis dilated cardiomyopathy heart failure

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The specificity of ten non-digestible carbohydrates to enhance butyrate-producing bacteria and butyrate production in vitro fermentation

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Food Science and Human Wellness 2023年第6期12卷 2344-2354页

作者： Jingjing Xu Ruyue Wang Weibao Liu zhongwei yin Jianrong Wu Xun Yu Wen Wang Hongtao Zhang Zhitao Li Minjie Gao Li Zhu Xiaobei Zhan Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China Wuxi Second People's Hospital Wuxi 214125China L&F Biotech.Ltd.7144 Collister Dr.Burnaby BC V5A3P6 Canada

Butyrate and butyrate-producing bacteria are important indicators of gut microbial metabolism in human health.Ten non-digestible carbohydrates(NDCs),including inulin,fructooligosaccharide(FOS),oatsβ-glucans(OGS),oatsβ-glucan oligosaccharides(OGOS),Astragalus polysaccharides(APS),Astragalus oligosaccharides(AOS),xanthan gum oligosaccharides(XGOS),gellan gum oligosaccharides(GGOS),curdlan oligosaccharides(COS),and pullulan oligosaccharides(POS)were used to investigate NDC specifi city in modulating butyrate-producing bacteria and butyrate production in 48 h in vitro fermentation studies in combination with fecal inocula from 7 healthy donors and 11 patients with type 2 diabetes(T2D).We observed that the amount of these ten NDCs utilized depended on NDC structure and inter-individual gut microbial differences.XGOS and GGOS fermentations signifi cantly increased butyrate-producing bacteria(especially f_Lachnospiraceae)and butyric acid production.Furthermore,XGOS and GGOS fermentations showed a better ability to consistently modulate gut microbiota composition and metabolic properties between individuals of healthy donors or T2D patients when compared to inulin,FOS,APS,AOS,OGS,OGOS,COS and POS fermentation.This research indicated that xanthan gum and gellan gum oligosaccharides have strong specifi city to enhance butyrate-producing bacteria and butyrate production.

关键词： Non-digestible carbohydrates Gut microbiota Butyric acid Butyrate-producing bacteria

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A Kaposi’s sarcoma-associated herpes virus-encoded microRNA contributes to dilated cardiomyopathy

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Signal Transduction and Targeted Therapy 2023年第7期8卷 3443-3454页

作者： Yanru Zhao Huaping Li Hengzhi Du zhongwei yin Mengying He Jiahui Fan Xiang Nie Yang Sun Huiying Hou Beibei Dai Xudong Zhang Yuanyuan Cai Kunying Jin Nan Ding Zheng Wen Jiang Chang Chen Chen Dao Wen Wang Division of Cardiology Department of Internal MedicineTongji HospitalTongji Medical CollegeHuazhong University of Science and Technology430030 WuhanChina Hubei Key Laboratory of Genetics and Molecular Mechanisms of Cardiological Disorders 430030 WuhanChina Division of Cardiology Department of Internal MedicineHubei Provincial Renmin Hospital430030 WuhanChina Division of Cardiology Department of Internal MedicineThe First People’s Hospital of Anqing246004 AnqingChina Department of Epidemiology and Biostatistics Key Laboratory for Environment and HealthSchool of Public HealthTongji Medical CollegeHuazhong University of Science and Technology430030 WuhanChina

Dilated cardiomyopathy(DCM)is the leading cause of heart transplantation.By microRNA(miRNA)array,a Kaposi’s sarcoma-associated herpes virus(KSHV)-encoded miRNA,kshv-miR-K12-1-5p,was detected in patients with DCM.The KSHV DNA load and kshv-miR-K12-1-5p level in plasma from 696 patients with DCM were measured and these patients were followed-up.

关键词： Kaposi cardiomyopathy sarcoma

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Human Vγ9Vδ2-T cells efficiently kill influenzavirus-infected lung alveolar epithelial cells

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Cellular & Molecular Immunology 2013年第2期10卷 159-164页

作者： Hong Li Zheng Xiang Ting Feng Jinrong Li yinping Liu yingying Fan Qiao Lu zhongwei yin Meixing Yu Chongyang Shen Wenwei Tu joint Research Center of West China Second University Hospital of Sichuan University and Department of Paediatrics and Adolescent Medicine of University of Hong Kong Sichuan University Chengdu 610041 China Department of Paediatrics and Adolescent Medicine University of Hong Kong Hong Kong China HL and ZX contributed equally to this study

γδ-T cells play an indispensable role in host defense against different viruses, including influenza A virus. However, whether these cells have cytotoxic activity against influenza virus-infected lung alveolar epithelial cells and subsequently contribute to virus clearance remains unknown. Using influenza virus-infected A549 cells, human lung alveolar epithelial cells, we investigated the cytotoxic activity of aminobisphosphonate pamidronate （PAM）-expanded human Vγ9Vδ2-T cells and their underlying mechanisms. We found that PAM could selectively activate and expand human Vγ9Vδ2-T cells. PAM-expanded human Vγ9Vδ2-T cells efficiently killed influenza virus-infected lung alveolar epithelial cells and inhibited virus replication. The cytotoxic activity of PAM-expanded Vγ9Vδ2-T cells was dependent on cell-to-cell contact and required NKG2D activation. Perforin-granzyme B, tumor-necrosis factor-related apoptosis-inducing ligand （TRAIL） and Fas-Fas ligand （FasL） pathways were involved in their cytotoxicity. Our study suggests that targeting γδ2-T cells by PAM can potentially offer an alternative option for the treatment of influenza virus.

关键词： A549 cells influenza A virus PAM Vγ9Vδ2 T cell

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Vγ9Vδ2-T lymphocytes have impaired antiviral function in small-for-gestational-age and preterm neonates

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Cellular & Molecular Immunology 2013年第3期10卷 253-260页

作者： Jinrong Li Hong Li Huawei Mao Meixing Yu Ting Feng Fan Yang yingying Fan Qiao Lu Chongyang Shen zhongwei yin Wenwei Tu Meng Mao The Joint Research Center of West China Second University Hospital of Sichuan University and Faculty of Medicine of the University of Hong Kong Chengdu China Department of Pediatric West China Second University Hospital Sichuan University Chengdu China Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education West China Second University Hospital Sichuan University Chengdu China Department of Pediatrics & Adolescent Medicine Li Ka Shing Faculty of Medicine The University of Hong Kong Hong Kong China These authors contributed equally to this work.

Preterm and small-for-gestational-age （SGA） neonates are vulnerable groups that are susceptible to various microbial infections. Vγ9Vδ2-T cells are critical components of the host immune system and have been demonstrated to play an important role in the defense against viral infection in adults. However, the characteristics of Vγ9Vδ2-T cells in children, especially the preterm and SGA populations, are poorly understood. Here, we examined the frequency and antiviral function of Vγ9Vδ2-T cells in neonates, including preterm, SGA and full-term babies. When compared to adults, neonates had a significantly lower percentage of Vγ9Vδ2-T cells in the blood. Upon influenza virus stimulation, neonatalVγ9Vδ2-T cells, especially from preterm and SGA babies, showed markedly decreased and delayed antiviral cytokine responses than those of adults. In addition, the antiviral responses of neonatal Vγ9Vδ2-T cells were positively correlated with gestational age and birth weight. Finally, a weaker expansion ofVγ9Vδ2-T cells by isopentenyl pyrophosphate （IPP） was shown in neonates than the expansion in adults. Our data suggest that the depressed antiviral activity and decreased frequency of Vγ9Vδ2-T cells may likely account for the high susceptibility to microbial infection in neonates, particularly in preterm and SGA babies. Improving Vγ9Vδ2-T -cell function of neonates may provide a new way to defend against virus infection.

关键词： cord blood influenza A virus preterm small for gestational age Vγ9Vδ2 T cell

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mpaired NK cell antiviral cytokine response against nfluenza virus in small-for-gestational-age neonates

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Cellular & Molecular Immunology 2013年第5期10卷 437-443页

作者： Jinrong Li Hong Li Huawei Mao Meixing Yu Fan Yang Ting Feng yingying Fan Qiao Lu Chongyang Shen zhongwei yin Meng Mao Wenwei Tu The Joint Research Center of West China Second University Hospital of Sichuan University and Faculty of Medicine of the University of Hong Kong Sichuan University Chengdu China Department of Pediatric West China Second University Hospital Sichuan University Chengdu China Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education West China Second University Hospital Sichuan University Chengdu China Department of Pediatrics & Adolescent Medicine Li Ka Shing Faculty of Medicine University of Hong Kong Pokfulam Hong Keng SAR. China These authors contributed equally to this work

The neonates, particularly small-for-gestational-age （SGA） ones, are susceptible to various microbial infections. Natural killer （NK） cells are critical components of host innate immunity system and the main source of the inflammatory cytokines, which provide critical protection during the early phase of viral infections before the development of an appropriate adaptive immune response. However, little is known about the antiviral effects of NK cells in neonates especially the SGA population. Herein, a prospective descriptive study was performed to determine the differences of NK cell immunity among adults, appropriate-for gestational-age （AGA） and SGA neonates. Adults have much higher NK cell number in peripheral blood than that in cord blood from neonates. In response to influenza virus stimulation, neonatal NK cells, especially SGA baby cells, expressed significantly lower antiviral cytokines including perforin, interferon （IFN）-7 and tumor-necrosis factor （TNF）-α responses than adult NK cells. In addition, the antiviral cytokine responses of NK cells were positively correlated with neonatal birth weight. Our data suggested that the depressed antiviral activity and less frequency of NK cells are likely to be responsible for the high susceptibility to microbial infection in neonates, at least in part. Improving the function of innate immunity may provide a new way to defend virus infection.

关键词： influenza A virus neonates NK cells SGA

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Real-time UWOC miniaturized system based on FPGA and LED arrays and its application in MIMO

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Chinese Optics Letters 2024年第2期22卷 70-75页

作者：黄安殷洪玺梁彦军王建英沈众卫 School of Information and Communication Engineering Dalian University of TechnologyDalian 116024China

In order to alleviate the impact of turbulence on the performance of underwater wireless optical communication(UWOC)in real time,and achieve high-speed real-time transmission and low cost and miniaturization of equipment,a 2×2 real-time multiple-input and multiple-output(MIMO)high-speed miniaturized UWOC system based on a field-programmable gate array(FPGA)and a high-power light-emitting diode(LED)array is designed in this Letter.In terms of multiplexing gain,the imaging MIMO spatial multiplexing and high-order modulation for the first time are combined and the real-time high-speed transmission of PAM-4 signal based on the LED array light source in 12 m underwater channel at 100 Mbps rate is implemented,which effectively improves the throughput of the UWOC system with a high-power commercial LED light source.In light of diversity gain,the system employs the diversity of repeated coding scheme to receive two identical non-return-to-zero on-off keying(NRZ-OOK)signals,which can compensate the fading or flickering sublinks in real time under the bubble-like simulated turbulence condition,and has high robustness.To our knowledge,this is the first instance of a high rate and long-distance implementation of a turbulence-resistant real-time MIMO miniaturized UWOC system based on FPGA and high-power LED arrays.With spatial diversity or spatial multiplexing capabilities,its low cost,integrity,and high robustness provide the system with important practical prospects.

关键词： underwater wireless optical communication MIMO spatial diversity spatial multiplexing

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蒸馏米酒蒸馏残液中多糖的分离及其益生活性研究

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食品与发酵工业 2022年第16期48卷 72-78页

作者：孙武徐静静尹忠伟朱莉詹晓北糖化学与生物技术教育部重点实验室(江南大学) 江苏无锡214122 A&F生物技术有限公司加拿大本拿比V5A3P6

蒸馏米酒在蒸馏过程中会产生大量的蒸馏残液,而蒸馏残液具有巨大的经济潜力。该研究从蒸馏米酒的蒸馏残液中分离纯化出一种分子质量为8554 Da的杂多糖并命名为CRWPs,CRWPs主要由葡萄糖、半乳糖、甘露糖、葡萄糖醛酸、半乳糖醛酸、岩藻... 详细信息

蒸馏米酒在蒸馏过程中会产生大量的蒸馏残液,而蒸馏残液具有巨大的经济潜力。该研究从蒸馏米酒的蒸馏残液中分离纯化出一种分子质量为8554 Da的杂多糖并命名为CRWPs,CRWPs主要由葡萄糖、半乳糖、甘露糖、葡萄糖醛酸、半乳糖醛酸、岩藻糖和阿拉伯糖组成,质量占比分别为91.56%、2.13%、1.61%、1.84%、1.34%、0.67%和0.12%。流变学分析表明CRWPs溶液表现出典型的剪切变稀行为,同时具有假塑性流体的特征。采用原子力显微镜(atomic force microscope,AFM)观察CRWPs在溶液-云母片表面的表观形貌,发现多糖链间聚合堆积而形成点状或棒状结构,推测该多糖具有多分支的结构。通过2株双歧杆菌和2株乳杆菌的静态发酵对CRWPs的益生功能进行评价。结果表明,与空白组比,CRWPs能够显著促进这4株菌的生长和短链脂肪酸的产生,其中短双歧杆菌的总酸产量从(105.62±2.70)mmol/mL提升到(145.55±5.36)mmol/mL,表明CRWPs具有一定的益生活性。该研究对从蒸馏残液中提取的多糖CRWPs的益生元潜力进行了报道,为其在环境经济型食品行业中的潜在应用奠定了基础。

关键词：蒸馏米酒蒸馏残液多糖益生元短链脂肪酸

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UHMWPE复合材料水润滑轴承润滑状态转变性能研究

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润滑与密封 2022年第5期47卷 98-104页

作者：王艳真王晔钟涛尹忠慰中国船舶及海洋工程设计研究院上海200011 上海交通大学设计学院上海200240

水润滑轴承润滑介质的黏度较低,轴承动压润滑难以形成。研究水润滑轴承润滑状态转变特性,可为水润滑复合材料轴承的设计和优化提供依据。建立水润滑轴承流固耦合计算模型,研究轴承承载力、水膜压力、轴承变形量随工况的变化关系,提出水... 详细信息

水润滑轴承润滑介质的黏度较低,轴承动压润滑难以形成。研究水润滑轴承润滑状态转变特性,可为水润滑复合材料轴承的设计和优化提供依据。建立水润滑轴承流固耦合计算模型,研究轴承承载力、水膜压力、轴承变形量随工况的变化关系,提出水膜厚度测试方法,研究轴承摩擦因数、水膜厚度随转速、负载的变化规律。研究结果表明:随偏心率和转速增大,轴承承载力、最大水膜压力和最大变形量均逐渐增大;随转速增大,轴承承载力、最大水膜压力和最大变形量的增幅逐渐减小。试验发现随着负载增大,改性UHMWPE轴承从混合润滑向动压润滑转变的膜厚比逐渐减小。

关键词： UHMWPE 水润滑轴承润滑状态动压润滑

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基于仿生胃肠道消化技术的不饱和脂肪酸体外发酵特性及其对肠道菌群影响研究

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食品与发酵工业 2023年第21期49卷 45-51页

作者：朱凌尹忠伟黎欢李志涛朱莉詹晓北教育部糖化学与生物技术重点实验室江南大学生物工程学院江苏无锡214122

脂肪酸是维持人体生存的必需营养物之一。国内外一致认为二十二碳六烯酸(docosahexaenoic acid,DHA)和二十碳四烯酸(arachidonic acid,AA)对大脑的发育具有重要的意义。肠道微生物与人体健康息息相关,然而对于不同浓度、不同饱和度的脂... 详细信息

脂肪酸是维持人体生存的必需营养物之一。国内外一致认为二十二碳六烯酸(docosahexaenoic acid,DHA)和二十碳四烯酸(arachidonic acid,AA)对大脑的发育具有重要的意义。肠道微生物与人体健康息息相关,然而对于不同浓度、不同饱和度的脂肪酸对肠道菌群的影响尚无相关研究。该研究从omega-3、6族中各挑选出代表性的脂肪酸,以DHA、AA、椰子油作为研究对象,通过油脂的乳化技术和体外动态仿生大肠反应器,对健康成人的肠道菌群进行体外厌氧发酵培养,探究了不同浓度、不同饱和度的脂肪酸对肠道菌群及代谢的影响。结果表明,2 mL/L DHA组中短链脂肪酸乙酸、丙酸、丁酸浓度分别达到了54.79、8.34、1.45 mmol/L,比2 mL/L阳性对照组分别变化了11.48%、-1.41%和66.98%,并且16S rDNA测序显示2 mL/L DHA组还稳定了肠道菌群,上调了不动杆菌属和双歧杆菌属等有益菌属。此外,低不饱和度脂肪酸较高不饱和度脂肪酸表现出更高的益生活性。该研究结果表明2 mL/L低饱和度的脂肪酸DHA对健康成人的肠道菌群具有正向影响。

关键词：不饱和脂肪酸饱和脂肪酸肠道菌群体外发酵厌氧发酵

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