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Abundance of antibiotic resistance genes and their association with bacterial communities in activated sludge of wastewater treatment plants: Geographical distribution and network analysis

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Journal of Environmental Sciences 2019年第8期31卷 24-38页

作者： Haihan zhang Huiyan He Shengnan Chen Tinglin Huang Kuanyu Lu zhonghui zhang Rong Wang Xueyao zhang Hailong Li Key Laboratory of Northwest Resource Environment and Ecology MOE Xi'an University of Architecture and Technology Shaanxi Key Laboratory of Environmental Engineering Xi'an University of Architecture and Technology Xi'an Environmental Monitoring Center Research Institute of Applied Biology Shanxi University

Wastewater treatment plants(WWTPs) are deemed reservoirs of antibiotic resistance genes(ARGs). Bacterial phylogeny can shape the resistome in activated sludge. However, the co-occurrence and interaction of ARGs abundance and bacterial communities in different WWTPs located at continental scales are still not comprehensively understood. Here, we applied quantitative PCR and Miseq sequence approaches to unveil the changing profiles of ARGs(sul1, sul2, tet W, tet Q, tet X), int I1 gene, and bacterial communities in 18 geographically distributed WWTPs. The results showed that the average relative abundance of sul1 and sul2 genes were 2.08 × 10^(-1) and 1.32 × 10^(-1) copies/16 S rRNA copies, respectively. The abundance of tet W gene was positively correlated with the Shannon diversity index(H′), while both studied sul genes had significant positive relationship with the int I1 gene. The highest average relative abundances of sul1, sul2, tet X, and int I1 genes were found in south region and oxidation ditch system. Network analysis found that 16 bacterial genera co-occurred with tet W gene. Co-occurrence patterns were revealed distinct community interactions between aerobic/anoxic/aerobic and oxidation ditch systems. The redundancy analysis model plot of the bacterial community composition clearly demonstrated that the sludge samples were significant differences among those from the different geographical areas,and the shifts in bacterial community composition were correlated with ARGs. Together,these findings from the present study will highlight the potential risks of ARGs and bacterial populations carrying these ARGs, and enable the development of suitable technique to control the dissemination of ARGs from WWTPs into aquatic environments.

关键词： Antibiotic resistance genes Activate sludge Bacterial community Network analysis Mobile genetic elements

A widely targeted metabolite modificomics strategy for modified metabolites identification in tomato

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Journal of Integrative Plant Biology 2024年第4期66卷 810-823页

作者： Jun Yang Ridong Chen Chao Wang Chun Li Weizhen Ye zhonghui zhang Shouchuang Wang School of Breeding and Multiplication(Sanya Institute of Breeding and Multiplication) Hainan UniversitySanya 572025China School of Tropical Agriculture and Forestry Hainan UniversityHaikou 572208China

The structural and functional diversity of plant metabolites is largely created via chemical modification of a basic ***,metabolite modifications in plants have still not been thoroughly investigated by metabolomics *** this study,a widely targeted metabolite modificomics(WTMM)strategy was developed based on ultra-high performance liquid chromatography-quadrupole-linear ion trap(UHPLC-Q-Trap)and UHPLC-Q-Exactive-Orbitrap(UHPLC-QE-Orbitrap),which greatly improved the detection sensitivity and the efficiency of identification of modified metabolites.A metabolite modificomics study was carried out using tomato as a model,and over 34,000 signals with MS2 information were obtained from approximately 232 neutral loss *** metabolite profiling was also performed by utilizing high-resolution mass spectrometry data to annotate a total of 2,118 metabolites with 125 modification types;of these,165 modified metabolites were identified in this ***,the WTMM database was used to assess diseased tomato tissues and 29 biomarkers were *** summary,the WTMM strategy is not only capable of large-scale detection and quantitative analysis of plant-modified metabolites in plants,but also can be used for plant biomarker development.

关键词： LC-MS metabolic diversity metabolite modificomics modified groups tomato

A Highly Stable Two-Photon Ratiometric Fluorescence Probe for Real-Time Biosensing and Imaging of Nitric Oxide in Brain Tissues and Larval Zebrafish

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CCS Chemistry 2022年第6期4卷 2020-2030页

作者： Zhiwen Gong Zhichao Liu zhonghui zhang Yuxiao Mei Yang Tian Shanghai Key Laboratory of Green Chemistry and Chemical Processes School of Chemistry and Molecular EngineeringEast China Normal UniversityShanghai 200241

Because nitric oxide(NO)plays important roles in nerve conduction,signal regulation,and immune protection,analysis of NO is of great significance for understanding the physiological and pathological processes related to neurological ***,a highly stable and selective two-photon ratiometric fluorescent probe was developed for real-time sensing and imaging of NO in neurons,brain tissues,and larval zebrafish,in which a Rhodamine B derivative(RBD)was designed for specific recognition of NO and gold nanoclusters(AuNCs)were synthesized as reference *** developed organic-inorganic nanoprobe exhibited high stability against biological thiol compounds and high selectivity against other reactive oxygen and nitrogen species,metal ions,and *** addition,the response time of the present nanoprobe was less than∼55 *** using the developed nanoprobe,we proved that hypoxia-induced neuronal death was regulated by ***,it was found that the hypoxia-induced NO increase in different brain regions was various and that the NO burst contributed to hypoxia-induced death of zebrafish.

关键词： nitric oxide two-photon fluorescence hypoxia brain neuron imaging

A New Micropropagation Technology of Tilia amurensis:In VitroMicropropagation of Mature Zygotic Embryos and the Establishment of a PlantRegeneration System

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Phyton-International Journal of Experimental Botany 2024年第2期93卷 277-289页

作者： Shijie Lin Zimo Wang Hongbo Zhu Conghui Wang Hongfeng Wang Dawei zhang Tianbing Gou Guangdao Bao Ye Luo Huaijiang He zhonghui zhang Jilin Provincial Academy of Forestry Sciences(Jilin Forestry Biological Control Central Station) Changchun130033China Institute of Forestry Inventory and Planning of Jilin Province Changchun130022China Jilin Forest Tree and Seeding Management Station Changchun130022China

Tilia amurensis is an economically valuable broadleaf tree species in Northeast *** production of *** varieties at commercial scales has been greatly limited by the low germination *** thus a pressing need to develop an organogenesis protocol for in vitro propagation of *** to alleviate ashortage of high-quality *** ***,we established a rapid in vitro propagation system *** from mature zygotic embryos and analyzed the effects of plant growth regulators and culture mediain different *** found that Woody plant medium(WPM)was the optimal primary culture medium formature zygotic *** highest callus induction percentage(68.76%)and number of axillary buds induced(3.2)were obtained in WPM+0.89μmol/L 6-benzyladenine(6-BA)+0.46μmol/L kinetin(KT)+0.25μmol/Lindole-3-butryic acid(IBA)+1.44μmol/L gibberellin A_(3)(GA_(3)).The multiple shoot bud development achievedthe highest percentage(83.32%)in the Murashige and Skoog(MS)+2.22μmol/L 6-BA+0.25μmol/L IBA+1.44μmol/L GA_(3).The rooting percentage(96.70%)was highest in 1/2 MS medium+1.48μmol/L *** percentage of transplanting plantlets was 82.22%in soil:vermiculite:perlite(5:3:1).Our study is the firstto establish an effective organogenesis protocol for *** using mature zygotic embryos.

关键词： Tilia amurensis Rupr. mature zygotic embryo micropropagation adventitious bud multiplication rooting

Core Competitiveness as the Fundamental Guarantee for Sustainable Development of Geo-parks

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Asian Agricultural Research 2013年第9期5卷 108-110,115页

作者： Chenxia SHI zhonghui zhang Henan Institute of Geological Survey Henan Shanshui Geological Tourism Resources Development Limited Company

With the booming development over the past years,geo-parks have achieved impressive progress together with a series of *** importantly,geological tourism is not in *** to prosper geological tourism and achieve the sustainable development of geoparks is the top concern of workers engaged in tourism geology in *** article discusses the problem from the perspective of core competitiveness of geo-parks,particularly from three aspects of subject,object and medium,and puts forward methods to form the core competitiveness.

关键词： Geo-park Geological tourism Core competitiveness S

OGG1 inhibition suppresses African swine fever virus replication

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Virologica Sinica 2023年第1期38卷 96-107页

作者： Jie Fan Xinqian Lv Saixia Yang Shuxian Geng Jifei Yang Yaru Zhao zhonghui zhang Zhijie Liu Guiquan Guan Jianxun Luo Qiaoying Zeng Hong Yin Qingli Niu College of Veterinary Medicine Gansu Agricultural UniversityLanzhou730070China African Swine Fever Regional Laboratory of China(Lanzhou) State Key Laboratory of Veterinary Etiological BiologyKey Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou730046China Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis Yangzhou UniversityYangzhou225009China

African swine fever virus(ASFV)is an important pathogen that causes a highly contagious and lethal disease in swine,for which neither a vaccine nor treatment is *** DNA repair enzyme 8-oxoguanine DNA glycosylase 1(OGG1),which excises the oxidative base lesion 8-oxo-7,8-dihydroguanine(8-oxoG),has been linked to the pathogenesis of different diseases associated with viral ***,the role of OGG1-base excision repair(BER)in ASFV infection has been poorly *** study aimed to characterize the alteration of host reactive oxygen species(ROS)and OGG1 and to analyse the role of OGG1 in ASFV *** found that ASFV infection induced high levels and dynamic changes in ROS and 8-oxoG and consistently increased the expression of *** yield,transcription level,and protein synthesis were reduced in ASFV-infected primary alveolar macrophages(PAMs)treated by TH5487 or SU0268 inhibiting *** expression of BER pathway associated proteins of ASFV was also suppressed in OGG1-inhibited ***,OGG1 was found to negatively regulate interferonβ(IFN-β)production during ASFV infection and IFN-βcould be activated by OGG1 inhibition with TH5487 and SU0268,which blocked OGG1 binding to ***,the interaction of OGG1 with viral MGF360-14-L protein could disturb IFN-βproduction to further affect ASFV *** results suggest that OGG1 plays the crucial role in successful viral infection and OGG1 inhibitors SU0268 or TH5487 could be used as antiviral agents for ASFV infection.

关键词： African swine fever virus(ASFV) 8-oxoguanine DNA glycosylase 1(OGG1) 8-oxo-7,8-dihydroguanine(8-oxoG) Inhibitors Antiviral effect

Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats

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Burns & Trauma 2023年第1期11卷 375-392页

作者： Taotao Hu Shusen Chang Fang Qi zhonghui zhang Jiayin Chen Lingli Jiang Dali Wang Chengliang Deng Kaiyu Nie Guangchao Xu Zairong Wei Department of Burns and Plastic Surgery Affiliated Hospital of Zunyi Medical UniversityNo.149 Dalian RoadHuichuan DistrictZunyiGuizhou 563003China The Collaborative Innovation Center of Tissue Damage Repair and Regeneration Medicine of Zunyi medical University No.6West Xuefu RoadXinpu DistrictZunyiGuizhou563003China

Background:Schwann cell-like cells(SCLCs),differentiated from mesenchymal stem cells,have shown promising outcomes in the treatment of peripheral nerve injuries in preclinical ***,certain clinical obstacles limit their ***,the primary aim of this study was to investigate the role of exosomes derived from SCLCs(SCLCs-exo)in peripheral nerve ***:SCLCs were differentiated from human amniotic mesenchymal stem cells(hAMSCs)in vitro and validated by immunofluorescence,real-time quantitative PCR and western blot *** derived from hAMSCs(hAMSCs-exo)and SCLCs were isolated by ultracentrifugation and validated by nanoparticle tracking analysis,WB analysis and electron microscopy.A prefab-ricated nerve graft was used to deliver hAMSCs-exo or SCLCs-exo in an injured sciatic nerve rat *** effects of hAMSCs-exo or SCLCs-exo on rat peripheral nerve injury(PNI)regeneration were determined based on the recovery of neurological function and histomorphometric *** effects of hAMSCs-exo or SCLCs-exo on Schwann cells were also determined via cell prolifer-ation and migration ***:SCLCs significantly expressed the Schwann cell markers glial fibrillary acidic protein and *** to hAMSCs-exo,SCLCs-exo significantly enhanced motor function recov-ery,attenuated gastrocnemius muscle atrophy and facilitated axonal regrowth,myelin forma-tion and angiogenesis in the rat ***,hAMSCs-exo and SCLCs-exo were effi-ciently absorbed by Schwann ***,compared to hAMSCs-exo,SCLCs-exo signifi-cantly promoted the proliferation and migration of Schwann ***-exo also significantly upregulated the expression of a glial cell-derived neurotrophic factor,myelin positive regulators(SRY-box transcription factor 10,early growth response protein 2 and organic cation/carnitine transporter 6)and myelin proteins(myelin basic protein and myelin protein zero)in Schwann ***:These findings

关键词： Schwann cell-like cells Exosomes Peripheral nerve injury Mesenchymal stem cells Nerve regeneration

Two gene clusters and their positive regulator SlMYB13 that have undergone domestication-associated negative selection control phenolamide accumulation and drought tolerance in tomato

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Molecular Plant 2024年第4期17卷 579-597页

作者： Peng Cao Jun Yang Linghao Xia zhonghui zhang Zeyong Wu Yingchen Hao Penghui Liu Chao Wang Chun Li Jie Yang Jun Lai Xianggui Li Meng Deng Shouchuang Wang School of Breeding and Multiplication(Sanya Institute of Breeding and Multiplication) Hainan UniversitySanya 572025China College of Tropical Agriculture and Forestry Hainan UniversityHaikou 572208China Collaborative Innovation Center of Nanfan and High-Efficiency Tropical Agriculture Hainan UniversityHaikou 572208China

Among plant metabolites,phenolamides,which are conjugates of hydroxycinnamic acid derivatives and polyamines,play important roles in plant adaptation to abiotic and biotic ***,the molecular mechanisms underlying phenolamide metabolism and regulation as well as the effects of domestication and breeding on phenolamide diversity in tomato remain largely *** this study,we performed a metabolite-based genome-wide association study and identified two biosynthetic gene clusters(BGC7 and BGC11)containing 12 genes involved in phenolamide metabolism,including four biosynthesis genes(two 4CL genes,one C3H gene,and one CPA gene),seven decoration genes(five AT genes and two UGT genes),and one transport protein gene(DTX29).Using gene co-expression network analysis we further discovered that SlMYB13 positively regulates the expression of two gene clusters,thereby promoting phenolamide *** and physiological analyses showed that BGC7,BGC11 and SlMYB13 enhance drought tolerance by enhancing scavenging of reactive oxygen species and increasing abscisic acid content in *** variation analysis suggested that BGC7,BGC11 and SlMYB13 were negatively selected during tomato domestication and improvement,leading to reduced phenolamide content and drought tolerance of cultivated ***,our study discovers a key mechanism of phenolamide biosynthesis and regulation in tomato and reveals that crop domestication and improvement shapes metabolic diversity to affect plant environmental adaptation.

关键词： tomato phenolamides gene cluster drought tolerance natural variation evolution

Fluorometric determination of mercury（Ⅱ） by using thymine-thymine mismatches as recognition elements,toehold binding,and enzyme-assisted signal amplification

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Fluorometric determination of mercury（Ⅱ） by using thymine...