Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats

作     者：Taotao Hu Shusen Chang Fang Qi Zhonghui Zhang Jiayin Chen Lingli Jiang Dali Wang Chengliang Deng Kaiyu Nie Guangchao Xu Zairong Wei 

作者机构：Department of Burns and Plastic SurgeryAffiliated Hospital of Zunyi Medical UniversityNo.149 Dalian RoadHuichuan DistrictZunyiGuizhou 563003China The Collaborative Innovation Center of Tissue Damage Repair and Regeneration Medicine of Zunyi medical UniversityNo.6West Xuefu RoadXinpu DistrictZunyiGuizhou563003China 

出 版 物：《Burns & Trauma》 (烧伤与创伤（英文）)

年 卷 期：2023年第11卷第1期

页      面：375-392页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：supported by the InnovationGroup Major Research Project of Guizhou Province Education Department(No.Qianjiaohe KY043) the Science and Technology Support Project of Guizhou Province(2020-5012) the PhD Fund of Scientific Research Foundation of the Affiliated Hospital of ZunyiMedical University(2020-03) the National Nature Science Foundation of China(81660325) the Collaborative Innovation Center of the Chinese Ministry of Education(2020-39) the Master Fund of Scientific Research Foundation of the Affiliated Hospital of Zunyi Medical University(2016-35) 

主　　题：Schwann cell-like cells Exosomes Peripheral nerve injury Mesenchymal stem cells Nerve regeneration 

摘      要：Background:Schwann cell-like cells(SCLCs),differentiated from mesenchymal stem cells,have shown promising outcomes in the treatment of peripheral nerve injuries in preclinical ***,certain clinical obstacles limit their ***,the primary aim of this study was to investigate the role of exosomes derived from SCLCs(SCLCs-exo)in peripheral nerve ***:SCLCs were differentiated from human amniotic mesenchymal stem cells(hAMSCs)in vitro and validated by immunofluorescence,real-time quantitative PCR and western blot *** derived from hAMSCs(hAMSCs-exo)and SCLCs were isolated by ultracentrifugation and validated by nanoparticle tracking analysis,WB analysis and electron microscopy.A prefab-ricated nerve graft was used to deliver hAMSCs-exo or SCLCs-exo in an injured sciatic nerve rat *** effects of hAMSCs-exo or SCLCs-exo on rat peripheral nerve injury(PNI)regeneration were determined based on the recovery of neurological function and histomorphometric *** effects of hAMSCs-exo or SCLCs-exo on Schwann cells were also determined via cell prolifer-ation and migration ***:SCLCs significantly expressed the Schwann cell markers glial fibrillary acidic protein and *** to hAMSCs-exo,SCLCs-exo significantly enhanced motor function recov-ery,attenuated gastrocnemius muscle atrophy and facilitated axonal regrowth,myelin forma-tion and angiogenesis in the rat ***,hAMSCs-exo and SCLCs-exo were effi-ciently absorbed by Schwann ***,compared to hAMSCs-exo,SCLCs-exo signifi-cantly promoted the proliferation and migration of Schwann ***-exo also significantly upregulated the expression of a glial cell-derived neurotrophic factor,myelin positive regulators(SRY-box transcription factor 10,early growth response protein 2 and organic cation/carnitine transporter 6)and myelin proteins(myelin basic protein and myelin protein zero)in Schwann ***:These findings