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SARS coronavirus papain-like protease inhibits the type I interferon signaling pathway through interaction with the STING-TRAF3- TBK1 complex

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Protein & Cell 2014年第5期5卷 369-381页

作者： Xiaojuan chen Xingxing Yang Yang Zheng Yudong Yang Yaling Xing zhongbin chen Division of Infection and Immunity Department of Electromagnetic and Laser Biology Beijing Institute of Radiation Medicine_ Beijing 100850 China Anhui Medical University Hefei 230032 China

SARS coronavirus （SARS-CoV） develops an antagonis- tic mechanism by which to evade the antiviral activities of interferon （IFN）. Previous studies suggested that SARS-CoV papain-like protease （PLpro） inhibits activa- tion of the IRF3 pathway, which would normally elicit a robust IFN response, but the mechanism（s） used by SARS PLpro to inhibit activation of the IRF3 pathway is not fully known. In this study, we uncovered a novel mechanism that may explain how SARS PLpro effi- ciently inhibits activation of the IRF3 pathway. We found that expression of the membrane-anchored PLpro domain （PLpro-TM） from SARS-CoV inhibits STING/ TBKl/IKKE-mediated activation of type I IFNs and dis- rupts the phosphorylation and dimerization of IRF3, which are activated by STING and TBKI. Meanwhile, we showed that PLpro-TM physically interacts with TRAF3, TBK1, IKK~, STING, and IRF3, the key components that assemble the STING-TRAF3-TBK1 complex for activa- tion of IFN expression. However, the interaction between the components in STING-TRAF3-TBK1 complex is dis- rupted by PLpro-TM. Furthermore, SARS PLpro-TM reduces the levels of ubiquitinated forms of RIG-I, STING, TRAF3, TBK1, and IRF3 in the STING-TRAF3- TBK1 complex. These results collectively point to a new mechanism used by SARS-CoV through which PLpro negatively regulates IRF3 activation by interaction withSTING-TRAF3-TBK1 complex, yielding a SARS-CoV countermeasure against host innate immunity.

关键词： SARS coronavirus papain-like protease,interferon deubiquitinase STING-TRAF3-TBK1 complex

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Coronavirus membrane-associated papain-like proteases induce autophagy through interacting with Beclinl to negatively regulate antiviral innate immunity

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Protein & Cell 2014年第12期5卷 912-927页

作者： Xiaojuan chen Kai Wang Yaling Xing Jian Tu Xingxing Yang Qian Zhao Kui Li zhongbin chen Division of Infection and Immunity Department of Electromagnetic and Laser Biology Beijing Institute of Radiation Medicine Beijing 100850 China Department of Immunology Bengbu Medical College Bengbu 233030 China Prince of Wales Clinical School The University of New South Wales Sydney NSW 2052 Australia Department of Microbiology Immunology and Biochemistry University of Tennessee Health Science Center Memphis TN 38163 USA

Autophagy plays important roles in modulating viral replication and antiviral immune response. Coronavirus infection is associated with the autophagic process, however, little is known about the mechanisms of autophagy induction and its contribution to coronavirus regulation of host innate responses. Here, we show that the membrane-associated papain-like protease PLP2 （PLP2-TM） of coronaviruses acts as a novel autophagy- inducing protein. Intriguingly, PLP2-TM induces incom- plete autophagy process by increasing the accumula- tion of autophagosomes but blocking the fusion of autophagosomes with lysosomes. Furthermore, PLP2- TM interacts with the key autophagy regulators, LC3 and Beclinl, and promotes Beclinl interaction with STING, the key regulator for antiviral IFN signaling. Finally, knockdown of Beclinl partially reverses PLP2-TM＇s inhibitory effect on innate immunity which resulting in decreased coronavirus replication. These results sug- gested that coronavirus papain-like protease induces incomplete autophagy by interacting with Beclinl, which in turn modulates coronavirus replication and antiviral innate immunity.

关键词： coronavirus papain-like proteaseautophagy antiviral immunity Beclinl STING

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Effect of antisense oligonucleotides targeting telomerase catalytic subunit on tumor cell proliferation in vitro

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Chinese Science Bulletin 2002年第12期47卷 993-997页

作者： Shengqi Wang Li Lin zhongbin chen Ruxian Lin Suhong chen Wei Guan Xiaohong Wang Beijing Inst Radiat Med Beijing 100850 Peoples R China

To screen specific antitumor drugs targeting telomerase catalytic subunit (hEST2), 12 different hEST2 antisense oligonucleotides were designed based on hEST2 mRNA second structure and transfected into tumor cell lines by the lipofectin-mediated method. Cell growth activity was evaluated by MTT assay. HEST212 was picked out and its specificity, antitumor tree and continuous effect were analyzed. The results showed that hEST212 had promising anti-tumor activity in vitro, hEST2 can be used as a pratical target and an antisense drug candidate for cancer.

关键词： telomerase HEST2 tumor antisense oligonucleotides.

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The Life-Cycle Model with Optimal Individual Years of Schooling and Simulation

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数学计算（中英文版） 2014年第2期3卷 44-48页

作者： Donghan Cai Huan Yang zhongbin chen College of Math. & Stat. Wuhan Univ. Wuhan 430072 PR. China School of Econ. & Management Wuhan Univ. Wuhan 430072 P.R. China

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A New Type Power Converter and Its Application Prospect in Industry

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Journal of Electromagnetic Analysis and Applications 2011年第3期3卷 94-100页

作者： Wenhua Hu Pinggang Song zhongbin chen Qianzhi Zhou 不详

A new type power convertsion mode is structured by combineing switch power conversion mode with linear amplifica-tion mode, so as to acquire their integrated optimal performances. The theoretical analyses and experimental results are presented in this paper. They are proved that the new type converter can match many kinds of loads, including nonlinear loads, with strong robustness, good waveforms and high efficiency. Its application prospect in industry can be expected, particularly for the conditions where serious static and dynamic performances are required.

关键词： Power Converter SLH Performances Nonlinear Loads Industry

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Pore shrinkage capacity of Shajiang black soils(Vertisols)on the North China Plain and its influencing factors

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Pedosphere 2024年第3期34卷 620-630页

作者： Yueming chen zhongbin ZHANG Lei GAO Zichun GUO Peng XIONG Fahui JIANG Xinhua PENG State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008(China) University of Chinese Academy of Sciences Beijing 100049(China) Jiangxi Agricultural University Nanchang 330045(China)

Different pore sizes present different pore shrinkage capacities in a nonrigid soil.However,the shrinkage capacities of different pore sizes and their influencing factors are not clear.We aimed to quantify the shrinkage capacities of different pore sizes(large pores,>50μm;medium pores,0.2-50μm;fine pores,<0.2μm)and determine how soil properties impact soil shrinkage capacity at the regional scale.Two sampling transects from west to east(360 km long,35 samples)and from north to south(190 km long,29 samples)were selected to investigate soil shrinkage capacity and physicochemical properties of at0-20 cm depth in the Vertisol(locally known as Shajiang black soil)region of the North China Plain.The results showed that soil total shrinkage capacity,indicated by the coefficient of linear extensibility(COLE),had a mean value of 0.041-0.051 in the west-east and north-south transects.Large pores had higher pore shrinkage index(PSI)values(0.103-0.109)than medium(0.077-0.096)and fine(0.087-0.091)pores.The PSI of fine pores showed a fluctuating increasing trend from northwest to southeast,and the fine pore shrinkage capacity determined the COLE(r^(2)=0.789,P<0.001).The PSI of large pores had a significant relationship with soil bulk density(r=0.281,P<0.05)and organic carbon(r=-0.311,P<0.05),whereas those of medium and fine pores were correlated with soil clay content(r=0.381 and 0.687,respectively,P<0.001).In addition,the PSI of fine pores was also correlated with montmorillonite content(r=0.387,P<0.01).It can be concluded that the PSI of large pores is related to anthropogenically influenced soil properties with low stability,whereas those of medium and fine pores are related to pedogenic properties.The high variability in anthropogenic and pedogenic factors explains the spatial pattern of Vertisol shrinkage capacity on the North China Plain.

关键词： clay content coefficient of linear extensibility pore shrinkage index soil structure spatial distribution swelling

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Proteolytic Processing,Interferon antagonist and Deubiquitinase Activies of the Papain-like Protease of the New Emerging Middle East Respiratory Syndrome Coronavirus

Proteolytic Processing,Interferon antagonist and Deubiquitin...

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第八届全国医学生物化学与分子生物学第五届全国临床应用生...

作者： Xingxing Yang Xiaojuan chen Yaling Xing zhongbin chen Division of Infection and Immunity Beijing Institute of Radiation Medicine Beijing Institute of Radiation Medicine Anhui Medical University

The newly emergent Middle East respiratory syndrome coronavirus(MERS-CoV)causes severe pulmonary disease in human beings,represents the second example of a highly pathogenic coronavirus followed by the first of SARS-CoV.Genomic studies revealed that only two viral proteases,papain-like protease(PLpro)and 3C-like protease(3CLpro),which process the polyproteins encoded by the MERS-CoV genomic RNA.We previously showed that SARS-CoV

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A general and facile calcination method to synthesize single-site catalysts for highly efficient electrochemical CO_(2) reduction

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Nano Research 2024年第5期17卷 3895-3901页

作者： Rui Sui Bingyan Wang Yongsheng Wang Jiajing Pei Wei Zhu Wenxing chen Chunhui Li Ailing Sun zhongbin Zhuang Engineering Research Center of Advanced Rare Earth Materials Department of ChemistryTsinghua UniversityBeijing 100084China College of Chemistry and Green Catalysis Center Zhengzhou UniversityZhengzhou 450001China State Key Lab of Organic-Inorganic Composites and Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical TechnologyBeijing 100029China Beijing Synchrotron Radiation Facility Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China Energy&Catalysis Center School of Materials Science and EngineeringBeijing Institute of TechnologyBeijing 100081China

The electrochemical CO_(2) reduction reaction(CO_(2)RR)has received widespread attention as a promising method for producing sustainable chemicals and mitigating the global warming.Here,we demonstrate a general and facile synthetic route for the metal-nitrogen-carbon(M-N-C)type catalyst by simply calcinating metal acetate and urea with commercial carbon black,which have potential application in CO_(2)RR.The synthesized Ni-NC-600 catalyst has the structure of single Ni atom coordinated with one N atom and three C atoms(Ni-N_(1)C_(3)),which is suggested by X-ray absorption spectroscopy.The Ni-NC-600 catalyst exhibits high CO_(2)RR catalytic performance and a high CO Faraday efficiency above 98%in a wide potential range from-0.7 to-1.3 V(vs.reversible hydrogen electrode(RHE)),superior to most of the reported Ni-N-C catalysts.This work has developed a facile strategy to synthesize high performance CO_(2)RR catalyst.

关键词： nickel single atom catalyst CO_(2)reduction reaction CO production electrocatalyst

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大型可变速发电电动机定转子绕组端部电动力仿真分析

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大电机技术 2024年第3期 16-22页

作者：王小建胡金明温占营陈忠宾肖先照李桂芬孙玉田江苏国信溧阳抽水蓄能发电有限公司江苏常州213300 哈尔滨电机厂有限责任公司哈尔滨150040 河北抚宁抽水蓄能有限公司河北秦皇岛066300

大型可变速发电电动机定转子绕组端部结构复杂,当电机出现突然短路等故障工况时,会在定转子端部绕组上产生强大的冲击电流,以致在绕组线棒上产生很大的电磁力。若线棒端部绑扎不牢,可能会引起线棒位移或变形,甚至出现绝缘损坏等故障。... 详细信息

大型可变速发电电动机定转子绕组端部结构复杂,当电机出现突然短路等故障工况时,会在定转子端部绕组上产生强大的冲击电流,以致在绕组线棒上产生很大的电磁力。若线棒端部绑扎不牢,可能会引起线棒位移或变形,甚至出现绝缘损坏等故障。本文对一台414MW可变速发电电动机的定转子绕组端部电动力进行了仿真分析,首先建立可变速发电电动机三维端部磁场的有限元仿真模型;然后计算得到可变速发电电动机在额定发电工况下发生两相突然短路、三相突然短路时的定转子电流,并将其作为有限元仿真的求解输入源;最后仿真计算可变速发电电动机定转子绕组端部电动力,并获得定转子绕组端部电动力在短路故障工况下的分布规律。

关键词：可变速发电电动机绕组电动力仿真短路工况

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Coronavirus papain-like proteases induce autophagy but block autophagosome fusion with lysosomes through interacting with Beclinl

Coronavirus papain-like proteases induce autophagy but block...

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细胞—生命的基础——中国细胞生物学学会2013年全国学...

作者： zhongbin chen Kai Wang Xiaojuan chen Yaling Xing Xingxing Yang Beijing Institute of Radiation Medicine

Autophagy is a cellular catabolic process that sequesters cargo in double-membraned autophagosome and delivers the cargo to lysosomes to be degraded for recycling resource for cells.Autophagy plays important roles in modulating viral replication and antiviral innate and adaptive immune response. Previous studies demonstrated that coronavirus infection is associated with the autophagic process,

关键词： Coronavirus NL63 Papain-like protease Autophagy Beclinl STING

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