Effect of antisense oligonucleotides targeting telomerase catalytic subunit on tumor cell proliferation in vitro

作     者：Shengqi Wang Li Lin Zhongbin Chen Ruxian Lin Suhong Chen Wei Guan Xiaohong Wang 

作者机构：Beijing Inst Radiat Med Beijing 100850 Peoples R China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2002年第47卷第12期

页      面：993-997页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This work was supported in part by the National Natural Science Foundation of China (Grant No. 39870879) the State "863" High-Tech Project (Grant Nos.102-08-04-01 and 2001AA215261) the Key Project from the Committee of Beijing Science Technology (Gra 

主　　题：telomerase HEST2 tumor antisense oligonucleotides. 

摘      要：To screen specific antitumor drugs targeting telomerase catalytic subunit (hEST2), 12 different hEST2 antisense oligonucleotides were designed based on hEST2 mRNA second structure and transfected into tumor cell lines by the lipofectin-mediated method. Cell growth activity was evaluated by MTT assay. HEST212 was picked out and its specificity, antitumor tree and continuous effect were analyzed. The results showed that hEST212 had promising anti-tumor activity in vitro, hEST2 can be used as a pratical target and an antisense drug candidate for cancer.