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Impact of clay stabilizer on the methane desorption kinetics and isotherms of Longmaxi Shale,China

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Petroleum Science 2022年第1期19卷 234-244页

作者： zhong-hua liu Jia-Chun Wang Bao-Jun Bai Yan-Ling Wang School of Petroleum Engineering China University of Petroleum(East China)Qingdao266580ShandongChina Exploration and Development Research Institute of Daqing Oilfield Co Ltd. Daqing163712HeilongjiangChina Geosciences and Geological and Petroleum Engineering Missouri University of Science and TechnologyRollaMO65409USA School of Petroleum and Natural Gas Engineering Chongqing University of Science and TechnologyChongqing401332China

Knowing methane desorption characteristics is essential to define the contribution of adsorbed gas to gas well *** evaluate the synthetic effect of a clay stabilizer solution on methane desorption kinetics and isotherms pertaining to Longmaxi shale,an experimental setup was designed based on the volumetric *** objective was to conduct experiments on methane adsorption and desorption kinetics and isotherms before and after clay stabilizer *** experimental data were a good fit for both the intraparticle diffusion model and the Freundlich isotherm *** analyzed the effect of the clay stabilizer on desorption kinetics and *** show that clay stabilizer can obviously improve the diffusion rate constant and reduce the methane adsorption ***,we analyzed the desorption efficiency before and after treatment as well as the adsorbed methane *** results show that a higher desorption efficiency after treatment can be observed when the pressure is higher than 6.84 ***,the adsorbed methane content before and after treatment all increase when the pressure decreases,and clay stabilizer can obviously promote the adsorbed methane to free gas when the pressure is lower than 19 *** can also be applied to the optimization formulation of slickwater and the design of gas well production.

关键词： Shale gas Clay stabilizer Kinetics Isotherms Adsorption Desorption efficiency

Resolution and Crystal Structure of 9α-(3-Azabicyclo[3,3,1]nonanyl)- 2′-cyclopentyl-2′-hydroxyl-2′-thienylacetate

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Chinese Journal of Structural Chemistry 2006年第5期25卷 547-551页

作者： liu He HAN Xiang-Yu ZHANG Yan-Ping zhong Bo-hua liu Ke-Liang Beijing Institute of Pharmacology and Toxicology 27 Taiping Road Beijing 100850 China

The crystal structure of 9α-（3-azabicyclo[3,3,1]nonanyl）-2′-cyclopentyl-2′-hydro- xyl-2′-thienylacetate （C16H27NO3S, Mr = 349.48） has been determined by single-crystal X-ray diffraction analysis. The crystal belongs to orthorhombic, space group P212121 with a = 14.937（3）, b = 8.1673（16）, c = 15.423（3） ,A, V = 1881.5（6） ,A^3, Z = 4, Dc =1.234 g/cm^3, μ = 0.188 mm^-1, F（000） = 752, the final R = 0.0468 and wR = 0.1251. The bicyclo[3,3,1]nonane ring system adopts the most favored twin-chair conformation. The crystal structure shows the existence of intramolecular O-H…O hydrogen bonds by which a one-dimensional chain structure is formed.

关键词： optical resolution crystal structure muscarinic receptor antagonist

Perioperative artificial nutrition in malnourished gastrointestinal cancer patients

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World Journal of Gastroenterology 2006年第15期12卷 2441-2444页

作者： Guo-Hao Wu zhong-hua liu Zhao-Han Wu Zhao-Guang Wu Department of General Surgery Z-hongshan-HospitalFudan University Shanghai 200032 China

AIM： To investigate the potential role of perioperative nutrition in reducing complications and mortality in malnourished gastrointestinal cancer patients. METHODS： Four hundred and sixty-eight elective moderately or severely malnourished surgical patients with gastric or colorectal cancers defined by the subjective global assessment （SGA） were randomly assigned to 7 d preoperative and 7 d postoperative parenteral or enteral nutrition vs a simple control group. The nutrition regimen included 24.6±5.2 kcal /kg per d non-protein and 0.23±0.04 g nitrogen /kg per d. Control patients did not receive preoperative nutrition but received 600±100 kcal non-protein plus or not plus 62 ± 16 g crystalline amino acids postoperatively. RESULTS： Complications occurred in 18.3% of the patients receiving nutrition and in 33.5% of the control patients （P= 0.012）. Fourteen patients died in the control group and 5 in those receiving nutrition. There were significant differences in the mortality between the two groups （2.1% vs 6.0%, P=0.003）. The total length of hospitalization and postoperative stay of control patients were significantly longer （29 vs 22 d, P=0.014） than those of the studied patients （23 vs 12 d, P= 0.000）. CONCLUSION： Perioperative nutrition support is beneficial for moderately or severely malnourished gastrointestinal cancer patients and can reduce surgical complications and mortality.

关键词： Malnutrition Perioperative nutrition Parenteral nutrition Enteral nutrition Mortality Complications

Rapid conversion of human ESCs into mouse ESC-like pluripotent state by optimizing culture conditions

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Protein & Cell 2012年第1期3卷 71-79页

作者： Qi Gu Jie Hao Xiao-yang Zhao Wei Li Lei liu liu Wang zhong-hua liu Qi Zhou State Key Laboratory of Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China College of Life Science Northeast Agricultural University of ChinaHarbin 150030China

The pluripotent state between human and mouse embryonic stem cells is *** state of human embryonic stem cells(ESCs)is believed to be primed and is similar with that of mouse epiblast stem cells(EpiSCs),which is different from the naïve state of mouse *** ESCs could be converted into a naïve state through exogenous expression of defined transcription factors(Hanna et al.,2010).Here we report a rapid conversion of human ESCs to mouse ESC-like naïve states only by modifying the culture *** converted human ESCs,which we called mhESCs(mouse ESC-like human ESCs),have normal karyotype,allow single cell passage,exhibit domed morphology like mouse ESCs and express some pluripotent markers similar with mouse *** the rapid conversion established a naïve pluripotency in human ESCs like mouse ESCs,and provided a new model to study the regulation of pluripotency.

关键词： human embryonic stem cells(hESCs) mouse ESCs naïve pluripotent state

Protection effect of Xuanfudaizhetang on reflux esophagitis in rats

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Asian Pacific Journal of Tropical Medicine 2014年第4期7卷 267-270页

作者： Bing Liang zhong-hua liu Hai-Tao Wei Zhi Zhang Gong-Ning Shi Department of Cardiothoracic Surgery Huahe HospitalHenan University Medical College Henan University

Objective:To study protection effect of Xuanfudaizhetang on reflux esophagitis in ***:A total of 50 Wistar rats were randomly divided into groups A,B,C,D and E with 10 in *** esophagitis model in rats was established by incomplete helicobacter seam+lower esophagus *** rats were divided into 5 groups:group A as control group,group B as model group,group C with saline lavage treatment,group D with motilium treatment,group E with Xuanfudaizhetang lavage *** of esophageal,gastric mucosa and pH changes of rats were compared between ***:Weight gain in group D and E was significantly higher in than group C;the esophageal mucosa grades and esophagus tissue pathological morphology grades of group D and E were higher than that of group B and C with significant difference between groups(P<0.05);pH of lower esophageal mucosa in group D and E increased significantly than that in the group B and C(P<0.05),and the distal mucosal pH dropped significantly in the group B and C(P<0.05).Conclusions:Xuanfudaizhetang can obviously improve the pH of lower esophageal mucosa in rats with reflux esophagitis,decrease pH value of gastric mucosal,thus improve esophageal mucosa pathological conditions to achieve therapeutic effect on reflux esophagitis.

关键词： Xuanfudaizhetang Reflux esophagitis Esophageal mucosa PH value Treatment

Effect of sevoflurane on tissue permeability of lung ischemia-reperfusion injury in rats

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Asian Pacific Journal of Tropical Medicine 2014年第4期7卷 276-279页

作者： Xiao-Hui Li zhong-hua liu Hong-Bing Ma Yong Li Hui Zhao Jian-Bo Che Wei-Chao liu Gong-Ning Shi huaihe River Hospital Henan University Medical School Henan University

Objective:To investigate the effect of sevoflurane on tissue permeability of lung ischemiareperfusion injury(LIRI)in ***:A total of 45 wistar rats were randomly divided into3 groupsⅠ,Ⅱ,Ⅲ.Modified Eppinger method was adopted to establish the rat lung ischemiareperfusion injury ***Ⅰserved as the control group,groupⅡas ischemia reperfusion group,groupⅢas sevoflurane ischemia-reperfusion *** gas index,lung permeability index(LPI)change,lung tissue pathology change and lung water content were observed and compared between groups of rats at different time ***:During ischemia reperfusion,all rats kept balance of the MAP during different time points,SPO_2 of groupⅡandⅢdecreased significantly thanⅠgroup(P<0.05);after reperfusion lung permeability index in GroupⅡandⅢwas higher than the control group significantly(P<0.05),120 min after reperfusion LPI change and iujury of groupⅢwas significantly lower thanⅡgroup(P<0.05);interstitial and alveolar cavity effusion in of groupⅢwere lower than that of groupⅡ.Conclusions:Sevoflurane pretreatment can reduce the lung tissue permeability,and LIRI plays a protective role in LIRI.

关键词： Sevoflurane Rats Lung ischemia-reperfusion Protection

Influence of As_2O_3 combined with ginsenosides Rg3 on inhibition of lung cancer NCI-H1299 cells and on subsistence of nude mice bearing hepatoma

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Asian Pacific Journal of Tropical Medicine 2014年第10期7卷 772-775页

作者： Jian-Bo Che zhong-hua liu Hong-Bing Ma Yong Li Hui Zhao Xiao-Hui Li Wei-Chao liu Gong-Ning Shi huaihe River Hospital Henan University

Objective:To study the effect of arsenic trioxide（As2O3）combined with ginsenosides Rg3 on inhibiting the NCI-H1299 lung cancer cells and subsistence in nude mice bearing ***:MTT method was used to measure the inhibition effect of（As2O3）combined Rg3 on NC1-H1299 cells,and the proliferation inhibiting effect was observed via establishing the transplanted tumor model in vitro.A total of 40 tumor-bearing nude mice were randomly divided into normal saline group,（As2O3）,Rg3 and As2O3+Rg3 *** tumor model of lung cancer in nude mice was constructed,followed by injection of certain concentrations of normal saline,As2O3,ginseng saponin Rg3 and As2O3+Rg3 every *** survival duration and the tumors size of the mice were recorded and the Kaplan-Meier curve was made;microscopic observation of apoptosis of tumor cells in vivo was done using TUNEL ***:After 72 h of *** rate of tumor cell in normal saline group,As2O3 ***3 group and As2O3+Rg3 group was（5.66±0.31）%,（65.58±4.75）%,（44.69±3.32）%and（82.67±5.43）%,*** rate of tumor cell in As2O3 ***3 groap and As2O3+Rg3 group was significantly higher than that of normal saline group（P<0.01）;inhibition rate of tumor cells of As2O3+Rg3 group was significantly higher than that of the two groups given As2O3 or Rg3 alone（P<0.01）.The tumor volume of As2O3 group,Rg3 group and As2O3+Rg3 group shrank to（65.38±3.25）%,（77.68±3.43）%and（42.65±3.55）%of the original,tumor volume of saline group was 1.21 times of the original size（P<0.01）;Median survival of saline group,Rg3 group,As2O3 group were significantly shorter than that of As2O3+Rg3 group（P<0.01）;co-ordinated intervention ability of As2O3+Rg3 on NCI-H1299 cell was significantly higher than that of As2O3 or Rg3,***:As2O3 combined with Rg3 can significantly inhibit prolifaration of NCI-H1299 cells in lung cancer,prolong survival fo tumor-bearing nude mice,and promote tumor cell

关键词： As_2O_3 Rg3 Lung cancer Combination therapy

Controlling stationary one-way steering in a three-level atomic ensemble

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Chinese Physics B 2023年第12期32卷 249-255页

作者：彭洁徐俊刘华忠赖章丽 Department of Basic Courses Wuhan Donghu UniversityWuhan 430071China College of Physical Science and Technology Central China Normal UniversityWuhan 430079China College of Mathematics and Physics Jinggangshan UniversityJi'an 343009China

We propose a scheme for establishing the stationary one-way quantum steering in a three-level Λ-type atomic ensemble. In our system, the cavity modes are generated from two atomic dipole-allowed transitions, which are in turn driven by two external classical fields. The atomic ensemble can act as an engineered reservoir to put two cavity modes into a squeezed state by two Bogoliubov dissipation pathways. When the damping rates of the two cavity modes are different,the steady-state one-way quantum steering of the intracavity and output fields is presented by adjusting the normalized detuning. The physical mechanism is analyzed based on a dressed state representation and Bogoliubov mode *** achieved optical one-way quantum steering scheme has potential applications in quantum secret information sharing protocols.

关键词： one-way quantum steering entanglement quantum correlation reservoir-engineered method

The construction of the siRNA expression vector and the silencing influence on FAK gene of Tca8113 cell line

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中国口腔颌面外科杂志 2008年第B5期6卷 154-155页

作者： Xing Shu zhong liu hua Lian Oral and Maxillofacial Surgery Stomatological College NanjingMedical University Hanzhong Lu 136Nanjing JiangSu210029China