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KAI1 is a potential target for anti-metastasis in pancreatic cancer cells

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World Journal of Gastroenterology 2008年第7期14卷 1126-1132页

作者： Jian-Hua Xu Xiao-zhong guo Li-Nan ren Li-Chun Shao Min-Pei Liu Department of Gastroenterology The General Hospital of Shenyang Military Command Shenyang 110016 Liaoning Province China

AIM: To investigate whether KAI1, as a metastasis suppressor gene, is associated with invasive and metastatic ability of pancreatic cancer ***: KAI1 gene was transfected into pancreatic cancer cell line MiaPaCa Ⅱ by liposomes selected with G418. Expression of transfected cells was measured by Western blotting, immunofluorescence and immunocytochemistry. Tumor cell invasion and metastatic ability were detected through gelatinase activity and reconstituted basement membrane (Matrigel) assay. pCMV-KAI1 was directly injected into the heterotopic human pancreatic adenocarcinoma successfully established in the groin of BALB/C nude mice, by subcutaneous injection of MiaPaCa Ⅱ pancreatic cancer cells. The statistical analysis between groups was determined by Student's two tailed t ***: By Western blotting, MiaPaCa Ⅱ cells transfected by KAI1 gene indicated KAI1 expression at approximately 29.1 kDa. Cytoplasm staining was positive and uniformly spread in transfected cancer cells, using immunohistochemistry and immunofluorescence. The most obvious difference was present after 30 h (MiaPaca Ⅱ 43.6 ± 9.42, pCMV-MiaPaca Ⅱ 44.8 ± 8.56, pCMV-KAI1-MiaPaca Ⅱ 22.0 ± 4.69, P rent in liver and lung between controls 1.62 ± 0.39, 0.45 ± 0.09, pCMV-KAI 1.01 ± 0.27, 0.33 ± 0.09 and KAI1-Lipofectamine 0.99 ± 0.21, 0.30 ± 0.09 respectively (P < 0.05).CONCLUSION: High expression of KAI1 gene was found in transfected MiaPaCa Ⅱ human pancreatic cancer cells with lower metastatic ability. KAI1 gene plays an important role in inhibiting metastasis of

关键词： KAI1 Pancreatic cancer cell line Transfection Immunocytochemistry Western blotting Immunofluorescence Gelatinolysis

Superconductivity of bilayer titanium/indium thin film grown on SiO2/Si(001)

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Chinese Physics B 2018年第6期27卷 429-433页

作者： Zhao-Hong Mo Chao Lu Yi Liu Wei Feng Yun Zhang Wen Zhang Shi-Yong Tan Hong-Jun Zhang Chun-Yu guo Xiao-Dong Wang Liang Wang Rui-Zhu Yang zhong-guo ren Xie-Gang Zhu zhong-Hua Xiong Qi An Xin-Chun Lai Institute of Materials China Academy of Engineering PhysicsJiangyou 621908China Department of Engineering and Applied Physics School of Physical SciencesUniversity of Science and Technology of ChinaHefei 230026China Zhejiang University Hangzhou 310058China China Academy of Engineering Physics Mianyang 621000China

Bilayer superconducting films with tunable transition temperature（Tc） are a critical ingredient to the fabrication of high-performance transition edge sensors. Commonly chosen materials include Mo/Au, Mo/Cu, Ti/Au, and Ti/Al systems. Here in this work, titanium/indium（Ti/In） bilayer superconducting films are successfully fabricated on SiO2/Si（001）substrates by molecular beam epitaxy（MBE）. The success in the epitaxial growth of indium on titanium is achieved by lowering the substrate temperature to-150？C during indium evaporation. We measure the critical temperature under a bias current of 10 μA, and obtain different superconducting transition temperatures ranging from 645 m K to 2.7 K by adjusting the thickness ratio of Ti/In. Our results demonstrate that the transition temperature decreases as the thickness ratio of Ti/In increases.

关键词： titanium/indium thin film molecular beam epitaxy (MBE) proximity effect

Hydrolysis of Phosphorodithioates with a Seven-membered Ring( Ⅰ )——Kinetics and Mechanism of Acidic Hydrolysis

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Chemical Research in Chinese Universities 1991年第3期7卷 188-192页

作者： zhong Diyuan, guo Hongyou, ren Xingang, Cao Weiliang, Xue Shilei and Wang Zouxin (Department of Applied Chemistry, Beijing Institute of Chemical Technology, Beijing)

Introduction The phosphorodithioate is an important organophosphate pesticide. The hydrolysis reactions are very important to the metabolism of the pesticides. There have been many papers published in this domain concerning the hydrolysis of organophosphate esters. Studies on the hydrolysis of phosphorodithioate esters with a seven-membered ring, however, have rarely been reported. O, O-biphenyl S-alkyl phosphorothiolothionates with notable biological activities have recently been synthesized. In the present pater, UV spectroscopic method and molecular orbital calculation are used to carry out a research into the kinetics and-mechanisms of their hydrolyses.

关键词： Kinetics Mechanism Acidic hydrolysis Phosphorodithioates

Morphological Mechanism and Experimental Verification of Self-healing of Basalt-fiber Modified Water-soaked Asphalt

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 171-186页

作者： XIAO Minmin DONG Jinyong LI Chunyan guo Xu ren Jianguo School of Urban Construction and Safety Engineering Shanghai Institute of TechnologyShanghai 201418China

This study investigates the mechanism of action of representative molecules of basalt fibers on the healing of water-soaked *** parameters,morphological characteristics,interfacial healing energy,and interfacial healing strength were analyzed using molecular dynamics and macroscopic tests under different time,temperature,and water conditions to evaluate the specific states and critical conditions involved in *** results indicate that basalt-fiber molecules can induce rearrangement and a combination of water-soaked asphalt at the healing *** groups with different bonding states increase the interfacial adsorption capacity of water-soaked *** interaction between basalt fiber molecules and water molecules leads to a"hoop"phenomenon,while aromatics-2 molecules exhibit a"ring band aggregation"*** former reduces the miscibility of water and asphalt molecules,while the latter causes slow diffusion of the ***,a micro-macro dual-scale comparison of interfacial healing strength was conducted at temperatures of 297.15 and 312.15 K to identify the strength transition point and critical temperature of 299.4 K during the self-healing process of basalt-fiber modified water-soaked asphalt.

关键词： water-soaked asphalt basalt-fibers self-healing properties moisture status molecular characterization

Exosomes:the next-generation therapeutic platform for ischemic stroke

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Neural Regeneration Research 2025年第5期20卷 1221-1235页

作者： Wenjing Yin Hongyin Ma Yang Qu Jiaxin ren Yingying Sun Zhen-Ni guo Yi Yang Stroke Center Department of NeurologyFirst Hospital of Jilin UniversityChangchunJilin ProvinceChina Neuroscience Research Center Department of NeurologyFirst Hospital of Jilin UniversityChangchunJilin ProvinceChina

Current therapeutic strategies for ischemic stroke fall short of the desired objective of neurological functional ***,there is an urgent need to develop new methods for the treatment of this *** are natural cell-derived vesicles that mediate signal transduction between cells under physiological and pathological *** have low immunogenicity,good stability,high delivery efficiency,and the ability to cross the blood–brain *** physiological properties of exosomes have the potential to lead to new breakthroughs in the treatment of ischemic *** rapid development of nanotechnology has advanced the application of engineered exosomes,which can effectively improve targeting ability,enhance therapeutic efficacy,and minimize the dosages *** in technology have also driven clinical translational research on *** this review,we describe the therapeutic effects of exosomes and their positive roles in current treatment strategies for ischemic stroke,including their antiinflammation,anti-apoptosis,autophagy-regulation,angiogenesis,neurogenesis,and glial scar formation reduction ***,it is worth noting that,despite their significant therapeutic potential,there remains a dearth of standardized characterization methods and efficient isolation techniques capable of producing highly purified *** optimization strategies should prioritize the exploration of suitable isolation techniques and the establishment of unified workflows to effectively harness exosomes for diagnostic or therapeutic applications in ischemic ***,our review aims to summarize our understanding of exosome-based treatment prospects in ischemic stroke and foster innovative ideas for the development of exosome-based therapies.

关键词： blood–brain barrier electroacupuncture engineering exercise exosomes ischemic stroke mesenchymal stem cells microglia neuroprotection stents

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芳纶复合材料对球形弹丸的抗贯穿性能研究

兵工学报 2010年第4期31卷 458-463页

作者：虢忠仁杜文泽钟蔚华金子明曲志敏宫平中国兵器工业第53研究所山东济南250031 装甲兵驻济南地区军事代表室山东济南250031

以芳纶纤维织物为增强材料、聚氨酯为树脂基体的芳纶复合材料,进行了2.05 g钨合金球、1.03 g钢球和4.50 g钢球的抗贯穿性能研究,并分析了芳纶复合材料对以上3种典型球形弹丸的抗弹性能差异。试验结果表明：芳纶复合材料的抗贯穿性能与... 详细信息

以芳纶纤维织物为增强材料、聚氨酯为树脂基体的芳纶复合材料,进行了2.05 g钨合金球、1.03 g钢球和4.50 g钢球的抗贯穿性能研究,并分析了芳纶复合材料对以上3种典型球形弹丸的抗弹性能差异。试验结果表明：芳纶复合材料的抗贯穿性能与测试过程中采用的弹丸种类有关,采用2.05 g钨球时较低,采用1.03 g钢球时较高,采用4.50 g钢球时居中。进而提出了一种新的芳纶复合材料抗贯穿性能表征方法,即直接作用面积比吸收能（PSEA）法。PSEA值在芳纶复合材料受到材质相同的球形弹丸冲击时基本相同,据此在芳纶复合材料对某种球形弹丸的弹道极限速度已知时,可计算出对其它球形弹丸的弹道极限速度。验证结果表明：弹道极限速度的计算值和实测值相差1%~6%.

关键词：爆炸力学芳纶复合材料钨合金球钢球抗弹机理直接作用面积比吸收能

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植物AP2/ERF类转录因子研究进展

遗传 2012年第7期34卷 835-847页

作者：张计育王庆菊郭忠仁江苏省中国科学院植物研究所南京210014 辽宁农业职业技术学院园林系营口115009

植物AP2/ERF是一个庞大的转录因子基因家族,含有由60~70个氨基酸组成的AP2/ERF结构域而得名,存在于所有的植物中。AP2/ERF转录因子参与多种生物学过程,包括植物生长、花发育、果实发育、种子发育、损伤、病菌防御、高盐、干旱等环境胁... 详细信息

植物AP2/ERF是一个庞大的转录因子基因家族,含有由60~70个氨基酸组成的AP2/ERF结构域而得名,存在于所有的植物中。AP2/ERF转录因子参与多种生物学过程,包括植物生长、花发育、果实发育、种子发育、损伤、病菌防御、高盐、干旱等环境胁迫响应等。AP2/ERF类转录因子参与水杨酸、茉莉酸、乙烯、脱落酸等多种信号转导途径,而且是逆境信号交叉途径中的连接因子。文章对国内外近年来有关植物AP2/ERF类转录因子的分类、生物学功能、基因调控等方面的研究进行了综述。

关键词： AP2/ERF转录因子生物学功能研究进展

低温诱导湖北海棠病程相关蛋白基因家族MhPRs的表达

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上海农业学报 2013年第1期29卷 20-23页

作者：张计育郭忠仁江苏省中国科学院植物研究所南京210014

以湖北海棠组培苗为材料,利用半定量RT-PCR研究了低温4℃处理后湖北海棠MhNPR 1、MhT-GA2以及病程相关蛋白基因家族成员(MhPR 1、MhPR 5、MhPR8和Mhchit 1)基因的表达量。结果表明:低温处理可以诱导这些基因的表达,并且所有基因的表达量... 详细信息

以湖北海棠组培苗为材料,利用半定量RT-PCR研究了低温4℃处理后湖北海棠MhNPR 1、MhT-GA2以及病程相关蛋白基因家族成员(MhPR 1、MhPR 5、MhPR8和Mhchit 1)基因的表达量。结果表明:低温处理可以诱导这些基因的表达,并且所有基因的表达量在48 h达到最大。可以推测:(1)低温作为一种逆境条件,可能使植物体内SA含量提高,诱导MhNPR1基因表达,使NPR1蛋白从细胞质进入细胞核,与TGA类转录因子相互作用,诱导下游病程相关蛋白基因表达;(2)低温可能直接诱导病程相关蛋白基因表达。

关键词：湖北海棠低温病程相关蛋白基因表达

氟化铅晶体的生长新技术及其Cherenkov辐射效应

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物理 2001年第8期30卷 496-500页

作者：沈定中任国浩中国科学院上海硅酸盐研究所上海201800

氟化铅晶体是一种性能优异的Cherenkov辐射材料 ,在实验高能物理领域被用作Cherenkov探测器 ,有广泛的应用前景 .但传统的生长方法不仅生长成本高 ,而且晶体性能差 ,难以满足实用要求 .文章介绍的掺脱氧剂非真空生长新技术既摆脱了真空... 详细信息

氟化铅晶体是一种性能优异的Cherenkov辐射材料 ,在实验高能物理领域被用作Cherenkov探测器 ,有广泛的应用前景 .但传统的生长方法不仅生长成本高 ,而且晶体性能差 ,难以满足实用要求 .文章介绍的掺脱氧剂非真空生长新技术既摆脱了真空法中设备投资大和工艺复杂的缺点 ,而且生长出的晶体性能优越 ,其透光率、吸收边、透光均匀性、能量分辨率和抗辐照损伤能力均可满足实用要求 ,处于世界领先水平 .

关键词：氟化铅 Cherenkov效应晶体生长非真空 Cherenkov探测器