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SARS-CoV-2 infection causes immunodeficiency in recovered patients by downregulating CD19 expression in B cells via enhancing B-cell metabolism

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Signal Transduction and Targeted Therapy 2021年第10期6卷 3119-3131页

作者： Yukai Jing Li Luo Ying Chen Lisa S.Westerberg Peng Zhou Zhiping Xu Andres A.Herrada Chan-Sik Park Masato Kubo Heng Mei Yu Hu Pamela Pui-Wah Lee Bing Zheng zhiwei sui Wei Xiao Quan Gong Zhongxin Lu Chaohong Liu Department of Medical Laboratory The Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and TechnologyWuhanPeople's Republic of China Department of Emergency Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalThird Hospital of Shanxi Medical UniversityTaiyuanPeople's Republic of China Tongji Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanPeople's Republic of China Department of Pathogen Biology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhanPeople's Republic of China Center for Biosafety Mega Science Wuhan Institute of VirologyChinese Academy of SciencesWuhanPeople's Republic of China Department of Microbiology Tumor and Cell Biology Karolinska InstituteStockholmSweden Wuhan Metware Biotechnology Co. LtdWuhanPeople's Republic of China Lymphatic and Inflammation Research Laboratory Facultad de Ciencias de la SaludInstituto de Ciencias BiomOdicasUniversidad Autdnoma de ChileTalcaChile Department of Pathology Asan Medical CenterUniversity of Ulsan College of MedicineSeoulKorea Laboratory for Cytokine Regulation Center for Integrative Medical Science(IMS)RIKEN Yokohama InstituteKanagawaJapan Institute of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanPeople's Republic of China Department of Paediatrics and Adolescent Medicine LKS Faculty of MedicineThe University of Hong KongHong KongPeople's Republic of China Department of Immunology School of MedicineYangtze UniversityJingzhouHubei ProvincePeople's Republic of China Clinical Molecular Immunology Center School of MedicineYangtze UniversityJingzhouHubei ProvincePeople's Republic of China Center for Advanced Measurement Science National Institute of MetrologyBeijingPeople's Republic of China Department of Respiratory The First Affiliated Hospital of Yangtze UniversityJingzhouHubei ProvincePeople's Republic of China

The SARS-CoV-2 infection causes severe immune ***,it is unclear if disrupted immune regulation still exists and pertains in recovered COVID-19 *** our study,we have characterized the immunephe no type of B cells from 15 recovered COVID-19 patients,and found that healthy controls and recovered patients had similar B-cell populations before and after BCR stimulation,but the frequencies of PBC in patients were significantly increased when compared to healthy controls before ***,the percentage of unswitched memory B cells was decreased in recovered patients but not changed in healthy controls upon BCR ***,we found that CD19 expression was significantly reduced in almost all the B-cell subsets in recovered ***,the BCR signaling and early B-cell response were disrupted upon BCR ***,we found that the reduced CD19 expression was caused by the dysregulation of cell *** conclusion,we found that SARS-CoV-2 infection causes immunodeficiency in recovered patients by downregulating CD19 expression in B cells via enhandng B-cell metabolism,which may provide a new intervention target to cure COVID-19.

关键词： patients metabolism CD19

ARTICLE OPEN Viral dynamics and antibody responses in people with asymptomatic SARS-CoV-2 infection

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Signal Transduction and Targeted Therapy 2021年第6期6卷 1788-1794页

作者： zhiwei sui Xinhua Dai Qingbin Lu Yulan Zhang Min Huang Shufen Li Tao Peng Jie Xie Yongzhuo Zhang Chunchen Wu Jianbo Xia Lianhua Dong Jiayi Yang Wenfeng Huang Siyuan Liu Ziquan Wang Ke Li Qingfang Yang Xi Zhou Ying Wu Wei Liu Xiang Fang Ke Peng Center for Advanced Measurement Science National Institute of MetrologyBeijingChina Department of Laboratorial Science and Technology School of Public HealthPeking UniversityBeijingChina State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhanHubeiChina Joint Laboratory of Infectious Diseases and Health Wuhan Institute of Virology and Wuhan Jinyintan HospitalWuhanChina Department of Laboratory Medicine Huazhong University of Science and TechnologyTongji HospitalTongji Medical CollegeHUSTWuhanHubeiChina Department of Laboratory Medicine Maternal and Child Health Hospital of Hubei ProvinceTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Shenzhen Mindray Bio-Medical Electronics Co. Ltd.ShenzhenChina University of Chinese Academy of Sciences BeijingChina State Key Laboratory of Virology/Institute of Medical Virology and School of Basic Medical Sciences Wuhan UniversityWuhanChina Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijingChina

Over 40% of the coronavirus disease 2019(COVID-19)COVID-19 patients were asymptomatically infected with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and the immune responses of these asymptomatic individuals is a critical factor for developing the strategy to contain the COVID-19 ***,we determined the viral dynamics and antibody responses among 143 asymptomatic individuals identified in a massive screening of more than 5 million people in eight districts of Wuhan in May *** individuals were admitted to the government-designated centralized sites in accordance with *** incidence rate of asymptomatic infection is〜2.92/100,*** individuals had low viral copy numbers(peaked at 315 copies/mL)and short-lived antibody responses with the estimated diminish time of 69 *** antibody responses in individuals with persistent SARS-CoV-2 infection is much longer with the estimated diminish time of 257 *** results imply that the immune responses in the asymptomatic individuals are not potent enough for preventing SARS-CoV-2 re-infection,which has recently been reported in recovered COVID-19 *** casts doubt on the efficacy of forming"herd-immunity"through natural SARS-CoV-2 infection and urges for the development of safe and effective vaccines.

关键词： asymptomatic admitted immunity

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X线图像的边缘增强和假彩色编码综合技术

杭州大学学报（自然科学版） 1989年第4期16卷 412-417页

作者：田志伟隋成华杭州大学物理学系

本文提出一种具有边缘效果的假彩色编码新方法,不仅能对黑白图像进行假彩色化处理,同时又能使图像边缘获得增强。并且处理的图像面积较大,可使用白光信息处理系统,操作方法简便。用以处理黑白X线胸片,对某些不易确诊的病症,如癌症等的... 详细信息

本文提出一种具有边缘效果的假彩色编码新方法,不仅能对黑白图像进行假彩色化处理,同时又能使图像边缘获得增强。并且处理的图像面积较大,可使用白光信息处理系统,操作方法简便。用以处理黑白X线胸片,对某些不易确诊的病症,如癌症等的鉴别诊断有很好的效果。

关键词： X射线图象边缘增强假彩色编码

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装载机驾驶室非线性减振系统试验与优化

中国机械工程 2022年第13期33卷 1529-1536页

作者：章新睢志伟李占龙秦园王瑶董荻赵钧铎太原科技大学机械工程学院太原030024

以某型装载机驾驶室减振系统为研究对象,通过优化解决了驾驶室振动过大和驾驶舒适性差的问题。以实测的车架振动信号为输入激励,根据驾驶室实际结构简化了驾驶室-座椅-人体的非线性减振系统模型,驾驶室和座椅的垂向振动加速度均方根值... 详细信息

以某型装载机驾驶室减振系统为研究对象,通过优化解决了驾驶室振动过大和驾驶舒适性差的问题。以实测的车架振动信号为输入激励,根据驾驶室实际结构简化了驾驶室-座椅-人体的非线性减振系统模型,驾驶室和座椅的垂向振动加速度均方根值的模型输出值和实车试验值最大误差不超过6%。以驾驶室和座椅的垂向加速度均方根值为目标,使用遗传算法进行多目标优化,将优化结果代入模型来验证优化前后的驾驶室与座椅的减振性能。实车试验表明,改进后驾驶室垂向加速度的均方根值减小16%,座椅垂向加速度的均方根值减小53%。

关键词：装载机驾驶室减振优化遗传算法

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转基因定量检测用质粒分子标准物质研究进展

生物技术通报 2012年第2期28卷 48-52页

作者：李亮王晶隋志伟臧超余笑波中国计量科学研究院北京100013 中国计量学院生命科学学院杭州310018

近10年来,应用于转基因植物产品定量检测的质粒分子标准物质凭借其易于富集,快捷高效等优点,成为各国检测机构的研究热点。对国内外转基因植物核酸定量检测用的质粒分子标准物质研究进展进行总结和评述,并且对该类标准物质在研制过程中... 详细信息

近10年来,应用于转基因植物产品定量检测的质粒分子标准物质凭借其易于富集,快捷高效等优点,成为各国检测机构的研究热点。对国内外转基因植物核酸定量检测用的质粒分子标准物质研究进展进行总结和评述,并且对该类标准物质在研制过程中的问题进行分析,同时展望了该类标准物质的发展与应用。

关键词：质粒分子标准物质均匀性稳定性不确定度核酸定量

流式分析技术快速定量检测牛乳中大肠杆菌O157:H7

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食品科学 2018年第6期39卷 302-306页

作者：刘思渊古少鹏隋志伟刘思章薛蕾霍乃蕊山西农业大学动物科技学院山西太谷030801 中国计量科学研究院北京100013

建立一种基于流式分析技术的快速定量检测牛乳中大肠杆菌O157:H7的方法。用偶联有异硫氰酸荧光素的大肠杆菌O157单克隆抗体对大肠杆菌O157:H7进行特异性标记,通过优化抗体反应条件,建立流式检测方法,然后对磷酸盐缓冲溶液(phosphate buf... 详细信息

建立一种基于流式分析技术的快速定量检测牛乳中大肠杆菌O157:H7的方法。用偶联有异硫氰酸荧光素的大肠杆菌O157单克隆抗体对大肠杆菌O157:H7进行特异性标记,通过优化抗体反应条件,建立流式检测方法,然后对磷酸盐缓冲溶液(phosphate buffer saline,PBS)和人工污染牛乳样品中不同浓度的大肠杆菌O157:H7进行定量检测。本研究建立的流式检测方法的在PBS中的检测范围为2.57×10~3~1.12×10~8 CFU/m L,灵敏度达到2.57×10~3 CFU/m L。将所建立的流式检测方法应用于牛乳样品检测,当人工污染牛乳样品中大肠杆菌O157:H7的浓度在2.31×10~4~1.48×10~8 CFU/m L之间时,流式检测方法与平板计数方法检测结果基本一致,方法的灵敏度为2.31×10~4 CFU/m L,检测时间为35 min。该方法能快速、定量地检测出牛乳样品中的大肠杆菌O157:H7,在食源性致病菌的快速筛查和监控中具有重要的应用价值。

关键词：流式分析技术快速检测大肠杆菌O157:H7 牛乳

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液压减振器节流孔与拖拉机减振特性研究

中国农机化学报 2022年第7期43卷 57-62页

作者：章新董荻睢志伟李占龙李建伟太原科技大学机械工程学院太原市030024

以某型拖拉机悬架系统液压减振器为研究对象,分析其复原阀与压缩阀节流孔截面积比对整车减振性能的影响。建立162个自由度的拖拉机整车Recurdyn动力学仿真模型和液压减振器AMEsim模型,并将两者进行联合仿真,在高速和低速工况,对比分析... 详细信息

以某型拖拉机悬架系统液压减振器为研究对象,分析其复原阀与压缩阀节流孔截面积比对整车减振性能的影响。建立162个自由度的拖拉机整车Recurdyn动力学仿真模型和液压减振器AMEsim模型,并将两者进行联合仿真,在高速和低速工况,对比分析等效阻尼系数相等条件下的节流孔截面积比大于、等于及小于1的3种方案对整车减振性能的影响。结果表明:为获得更好的整车舒适性,对于常在低速工况作业的农用车辆,液压减振器节流孔截面积比值适宜小于1;常在较高和高速工况行驶的车辆,节流孔截面积比值适宜大于1。该设计原则可应用于拖拉机和其他车辆液压减振器节流孔截面积比的选取。

关键词：拖拉机液压减振器节流孔截面积比联合仿真 AMEsim 组合优化

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转基因油菜Ms8质粒分子的制备

浙江农业学报 2017年第5期29卷 694-700页

作者：余笑波沙跃兵张江东赵雷陈怡隋志伟浙江省计量科学研究院浙江杭州310018 中国计量科学研究院北京100013

随着转基因产品的大规模商业化和我国大规模进口油菜籽,有效管控存在的风险显得非常必要和有意义,试验研究了质粒标准分子pMs8的研制过程,包括质粒分子的构建、纯度分析、特异性检测、均匀性检验、稳定性考察、定值。结果表明,该质粒分... 详细信息

随着转基因产品的大规模商业化和我国大规模进口油菜籽,有效管控存在的风险显得非常必要和有意义,试验研究了质粒标准分子pMs8的研制过程,包括质粒分子的构建、纯度分析、特异性检测、均匀性检验、稳定性考察、定值。结果表明,该质粒分子标准物质的内源基因和外源基因的均匀性良好,没有扩增出所选的其他特异性基因,短期稳定性在14 d(处理温度大于25℃)发生显著变化,长期稳定性大于6个月,两种方法对外源基因拷贝数的定值结果相近,因此,p Ms8是有准确量值的质粒标准分子。可替代性研究结果表明,其可替代基因组作为转基因油菜Ms8定量的阳性标准。

关键词：转基因油菜数字PCR 质粒标准分子 Ms8

The regulators of BCR signaling during B cell activation

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Blood Science 2019年第2期1卷 119-129页

作者： Yue Wen Yukai Jing Lu Yang Danqing Kang Panpan Jiang Na Li Jiali Cheng Jingwen Li Xingbo Li Zican Peng Xizi Sun Heather Miller zhiwei sui Quan Gong Boxu Ren Wei Yin Chaohong Liu Department of Pathogen Biology School of Basic MedicineHuazhong University of Science and TechnologyWuhanChina Department of Medical Imaging Technology Clinical and Molecular Immunology Research CenterMedical school of Yangtze UniversityJingzhouHubeiChina Department of Intracellular Pathogens National Institute of Allergy and Infectious DiseasesMT 59840USA Division of Medical and Biological Measurement National Institute of MetrologyBeijingChina Wuhan Children’s Hospital Tongji Medical CollegeHuazhong University of Science&TechnologyWuhanChina

B lymphocytes produce antibodies under the stimulation of specific antigens,thereby exerting an immune effect.B cells identify antigens by their surface B cell receptor(BCR),which upon stimulation,directs the cell to activate and differentiate into antibody generating plasma *** of B cells via their BCRs involves signaling pathways that are tightly controlled by various *** this review,we will discuss three major BCR mediated signaling pathways(the PLC-g2 pathway,PI3K pathway and MAPK pathway)and related regulators,which were roughly divided into positive,negative and mutual-balanced regulators,and the specific regulators of the specific signaling pathway based on regulatory effects.

关键词： BCR MAPK PI3K PLC-g2 Regulator Signaling pathways