检索结果-南通市图书馆

Comparison of concentration methods for detection of hepatitis A virus in water samples

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Virologica Sinica 2016年第4期31卷 331-338页

作者： Yuting Qiao zhiwei sui Guoliang Hu Huabin Cao Guoxiang Yang Yong Li Yongsong Lei Lihua Zhao Quanjiao Chen CAS Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China National Institute of Metrology Beijing 100013 China College of Animal Science and Technology Institute of Animal Population Health Jiangxi Agricultural University Nanchang 330045 China Hubei Wildlife Epidemic Focus and Disease Surveillance Center Wuhan 430075 China

Hepatitis A virus is a pathogen associated with water pollution.Contaminated drinking water can cause hepatitis A outbreaks,lead to economic losses,and even threaten human lives.It is difficult to detect low levels of hepatitis A virus in water,so the virus must be concentrated in order to quantify it accurately.Here,we present a simple,rapid,efficient technique for the concentration and detection of hepatitis A virus in water.Our data showed that adding phosphate-buffered saline to the water,pre-filtering the water,and adding Trizol reagent directly to the filtration membrane can significantly improve concentration efficiency.Of three types of filtration membranes studied(mixed cellulose ester membrane,polyvinylidene fluoride membrane,and nylon membrane),the concentration efficiency using mixed cellulose ester membrane with a 0.1-μm pore size was the highest,reaching 92.62 ± 5.17%.This method was used to concentrate hepatitis A virus in water samples from Donghu Lake.Using SYBR Green real-time reverse transcription polymerase chain reaction analysis,the detection sensitivity of this method reached 10~1 copies/μL and its concentration efficiency reached 79.45 ± 9.88%.

关键词： concentration filtration membrane hepatitis A virus mixed cellulose ester(MCE) membrane water samples

维普期刊数据库

机器学习在超临界二氧化碳制膜技术教学中的应用实践

在线全文

学校读者我要写书评

暂无评论

电镀与涂饰 2024年第6期43卷 93-101页

作者：眭志强李树华林璟杨伟梁红蔡史佳乔智威广州大学化学化工学院广东广州510006

[目的]探索将超临界二氧化碳(CO_(2))辅助制备金属-有机框架(MOF)膜与机器学习技术相结合的创新教学模式,以提高本科生在实验数据处理、分析和预测方面的能力。[方法]在教学过程中,学生首先使用数据提取工具从复杂的实验图表中提取CO_(2... 详细信息

[目的]探索将超临界二氧化碳(CO_(2))辅助制备金属-有机框架(MOF)膜与机器学习技术相结合的创新教学模式,以提高本科生在实验数据处理、分析和预测方面的能力。[方法]在教学过程中,学生首先使用数据提取工具从复杂的实验图表中提取CO_(2)在MOF材料上的吸附等温线数据,构建包含数据点和材料性能参数的数据集。随后,学生应用极致梯度提升(XGB)和随机森林(RF)两种机器学习算法对提取的数据集进行训练和测试,建立材料性能预测模型。[结果]数据提取工具显著提高了数据处理的效率和准确性,XGB模型在材料性能参数预测方面表现出良好的准确性和可解释性。通过这种结合数据提取和机器学习的教学模式,学生在数据处理和预测方面取得了显著进步,增强了他们的数据分析和科研能力。[结论]本工作提出的教学模式为化学工程和材料科学领域的实验数据处理和分析提供了有效的解决方案,具有较高的应用价值。

关键词：金属-有机框架超临界二氧化碳制膜技术机器学习数据提取

维普期刊数据库博看期刊

在线全文

学校读者我要写书评

暂无评论

双轴扫描声呐在桥墩冲刷监测中的应用

自动化与仪器仪表 2024年第4期 266-270页

作者：张龙隋海琛孙辉崔志伟交通运输部天津水运工程科学研究所天津300456 天津水运工程勘察设计院有限公司天津市水运工程测绘技术重点实验室天津300456

针对潜水探摸费用高、作业时间受限,传统光栅监测不直观及多波束测深监测在实时性连续性上存在缺陷,采用定点静态监测方法,将两台双轴扫描声呐设备固定安装在指定桥墩上,声呐探头以一定的水平角度和垂直角度步进旋转,在水平、垂直旋转... 详细信息

针对潜水探摸费用高、作业时间受限,传统光栅监测不直观及多波束测深监测在实时性连续性上存在缺陷,采用定点静态监测方法,将两台双轴扫描声呐设备固定安装在指定桥墩上,声呐探头以一定的水平角度和垂直角度步进旋转,在水平、垂直旋转指定角度区间形成一幅完整的三维点云图像。采集的数据由串口服务器转为网络数据后通过4G DTU远程传输给数据接收终端进行处理、汇总、分析、成图、显示。通过软硬件集成系统实现大范围、长时间、高频次、高精度的无人值守冲刷监测,避免了探头中途拆装等操作对前后所测数据的一致性产生的可能影响。同时,固定安装的声呐系统克服了其他监测手段数据不直观、不及时等缺陷,可按照指定时间获取面状的全覆盖海底地形数据,对桥墩周边海床基础数据进行实时冲刷监测,监测系统运行稳定,远程采集控制及回传数据正常,数据精度可达0.1 m,数据点密度可达1 m×1 m。数学模型对冲刷坑的冲刷深度和范围与实测值相比,结果吻合。

关键词：桥墩冲刷局部冲刷双轴扫描声呐监测系统杭州湾大桥

维普期刊数据库

The regulators of BCR signaling during B cell activation

在线全文

学校读者我要写书评

暂无评论

Blood Science 2019年第2期1卷 119-129页

作者： Yue Wen Yukai Jing Lu Yang Danqing Kang Panpan Jiang Na Li Jiali Cheng Jingwen Li Xingbo Li Zican Peng Xizi Sun Heather Miller zhiwei sui Quan Gong Boxu Ren Wei Yin Chaohong Liu Department of Pathogen Biology School of Basic MedicineHuazhong University of Science and TechnologyWuhanChina Department of Medical Imaging Technology Clinical and Molecular Immunology Research CenterMedical school of Yangtze UniversityJingzhouHubeiChina Department of Intracellular Pathogens National Institute of Allergy and Infectious DiseasesMT 59840USA Division of Medical and Biological Measurement National Institute of MetrologyBeijingChina Wuhan Children’s Hospital Tongji Medical CollegeHuazhong University of Science&TechnologyWuhanChina

B lymphocytes produce antibodies under the stimulation of specific antigens,thereby exerting an immune effect.B cells identify antigens by their surface B cell receptor(BCR),which upon stimulation,directs the cell to activate and differentiate into antibody generating plasma cells.Activation of B cells via their BCRs involves signaling pathways that are tightly controlled by various regulators.In this review,we will discuss three major BCR mediated signaling pathways(the PLC-g2 pathway,PI3K pathway and MAPK pathway)and related regulators,which were roughly divided into positive,negative and mutual-balanced regulators,and the specific regulators of the specific signaling pathway based on regulatory effects.

关键词： BCR MAPK PI3K PLC-g2 Regulator Signaling pathways

SARS-CoV-2 infection causes immunodeficiency in recovered patients by downregulating CD19 expression in B cells via enhancing B-cell metabolism

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2021年第10期6卷 3119-3131页

作者： Yukai Jing Li Luo Ying Chen Lisa S.Westerberg Peng Zhou Zhiping Xu Andres A.Herrada Chan-Sik Park Masato Kubo Heng Mei Yu Hu Pamela Pui-Wah Lee Bing Zheng zhiwei sui Wei Xiao Quan Gong Zhongxin Lu Chaohong Liu Department of Medical Laboratory The Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and TechnologyWuhanPeople's Republic of China Department of Emergency Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalThird Hospital of Shanxi Medical UniversityTaiyuanPeople's Republic of China Tongji Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanPeople's Republic of China Department of Pathogen Biology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhanPeople's Republic of China Center for Biosafety Mega Science Wuhan Institute of VirologyChinese Academy of SciencesWuhanPeople's Republic of China Department of Microbiology Tumor and Cell Biology Karolinska InstituteStockholmSweden Wuhan Metware Biotechnology Co. LtdWuhanPeople's Republic of China Lymphatic and Inflammation Research Laboratory Facultad de Ciencias de la SaludInstituto de Ciencias BiomOdicasUniversidad Autdnoma de ChileTalcaChile Department of Pathology Asan Medical CenterUniversity of Ulsan College of MedicineSeoulKorea Laboratory for Cytokine Regulation Center for Integrative Medical Science(IMS)RIKEN Yokohama InstituteKanagawaJapan Institute of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanPeople's Republic of China Department of Paediatrics and Adolescent Medicine LKS Faculty of MedicineThe University of Hong KongHong KongPeople's Republic of China Department of Immunology School of MedicineYangtze UniversityJingzhouHubei ProvincePeople's Republic of China Clinical Molecular Immunology Center School of MedicineYangtze UniversityJingzhouHubei ProvincePeople's Republic of China Center for Advanced Measurement Science National Institute of MetrologyBeijingPeople's Republic of China Department of Respiratory The First Affiliated Hospital of Yangtze UniversityJingzhouHubei ProvincePeople's Republic of China

The SARS-CoV-2 infection causes severe immune disruption.However,it is unclear if disrupted immune regulation still exists and pertains in recovered COVID-19 patients.In our study,we have characterized the immunephe no type of B cells from 15 recovered COVID-19 patients,and found that healthy controls and recovered patients had similar B-cell populations before and after BCR stimulation,but the frequencies of PBC in patients were significantly increased when compared to healthy controls before stimulation.However,the percentage of unswitched memory B cells was decreased in recovered patients but not changed in healthy controls upon BCR stimulation.Interestingly,we found that CD19 expression was significantly reduced in almost all the B-cell subsets in recovered patients.Moreover,the BCR signaling and early B-cell response were disrupted upon BCR stimulation.Mechanistically,we found that the reduced CD19 expression was caused by the dysregulation of cell metabolism.In conclusion,we found that SARS-CoV-2 infection causes immunodeficiency in recovered patients by downregulating CD19 expression in B cells via enhandng B-cell metabolism,which may provide a new intervention target to cure COVID-19.

关键词： patients metabolism CD19

Hyperparameter Optimization for Capsule Network Based Modified Hybrid Rice Optimization Algorithm

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Intelligent Automation & Soft Computing 2023年第8期37卷 2019-2035页

作者： zhiwei Ye Ziqian Fang Zhina Song Haigang sui Chunyan Yan Wen Zhou Mingwei Wang School of Computer Science and Technology Hubei University of TechnologyWuhan430068China State Key Laboratory of Information Engineering in Surveying Mapping and Remote SensingWuhan UniversityWuhan430079China

Hyperparameters play a vital impact in the performance of most machine learning algorithms.It is a challenge for traditional methods to con-figure hyperparameters of the capsule network to obtain high-performance manually.Some swarm intelligence or evolutionary computation algorithms have been effectively employed to seek optimal hyperparameters as a com-binatorial optimization problem.However,these algorithms are prone to get trapped in the local optimal solution as random search strategies are adopted.The inspiration for the hybrid rice optimization(HRO)algorithm is from the breeding technology of three-line hybrid rice in China,which has the advantages of easy implementation,less parameters and fast convergence.In the paper,genetic search is combined with the hybrid rice optimization algorithm(GHRO)and employed to obtain the optimal hyperparameter of the capsule network automatically,that is,a probability search technique and a hybridization strategy belong with the primary HRO.Thirteen benchmark functions are used to evaluate the performance of GHRO.Furthermore,the MNIST,Chest X-Ray(pneumonia),and Chest X-Ray(COVID-19&pneumonia)datasets are also utilized to evaluate the capsule network learnt by GHRO.The experimental results show that GHRO is an effective method for optimizing the hyperparameters of the capsule network,which is able to boost the performance of the capsule network on image classification.

关键词： Hyperparameter optimization hybrid rice optimization algorithm genetic algorithm capsule network image classification

基于文献计量学的国家重大科研仪器研制项目资助成效研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

计量科学与技术 2024年第5期68卷 110-120页

作者：李晓萌商学棽范雯隋志伟李大博吴爱华刘玉兰刘羿彤中国计量科学研究院中国仪器仪表学会分析仪器分会

科研仪器是科技创新的基础条件和成果，在新一轮科技革命和产业变革的形势下，已成为包括我国在内的世界各国科技创新竞争的焦点。以国家重大科研仪器研制项目（以下简称“仪器研制项目”）中的科研产出作为研究对象，采用文献计量学分... 详细信息

科研仪器是科技创新的基础条件和成果，在新一轮科技革命和产业变革的形势下，已成为包括我国在内的世界各国科技创新竞争的焦点。以国家重大科研仪器研制项目（以下简称“仪器研制项目”）中的科研产出作为研究对象，采用文献计量学分析，通过科技论文的产出数量、出版期刊、被引频次、产出机构等不同维度揭示其数量、质量特征和内在规律。同时，结合高被引论文与热点论文，对2011—2022年度仪器研制项目的产出情况、产出影响力、投入产出比等多个维度进行资助成效分析。以期为科学基金项目的成果产出绩效评价提供依据，为了解仪器基金在推进我国基础研究前沿突破中发挥的重要作用提供参考价值。

关键词：计量学重大科研仪器文献计量学科研成果科技论文资助成效

ARTICLE OPEN Viral dynamics and antibody responses in people with asymptomatic SARS-CoV-2 infection

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2021年第6期6卷 1788-1794页

作者： zhiwei sui Xinhua Dai Qingbin Lu Yulan Zhang Min Huang Shufen Li Tao Peng Jie Xie Yongzhuo Zhang Chunchen Wu Jianbo Xia Lianhua Dong Jiayi Yang Wenfeng Huang Siyuan Liu Ziquan Wang Ke Li Qingfang Yang Xi Zhou Ying Wu Wei Liu Xiang Fang Ke Peng Center for Advanced Measurement Science National Institute of MetrologyBeijingChina Department of Laboratorial Science and Technology School of Public HealthPeking UniversityBeijingChina State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhanHubeiChina Joint Laboratory of Infectious Diseases and Health Wuhan Institute of Virology and Wuhan Jinyintan HospitalWuhanChina Department of Laboratory Medicine Huazhong University of Science and TechnologyTongji HospitalTongji Medical CollegeHUSTWuhanHubeiChina Department of Laboratory Medicine Maternal and Child Health Hospital of Hubei ProvinceTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Shenzhen Mindray Bio-Medical Electronics Co. Ltd.ShenzhenChina University of Chinese Academy of Sciences BeijingChina State Key Laboratory of Virology/Institute of Medical Virology and School of Basic Medical Sciences Wuhan UniversityWuhanChina Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijingChina

Over 40% of the coronavirus disease 2019(COVID-19)COVID-19 patients were asymptomatically infected with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and the immune responses of these asymptomatic individuals is a critical factor for developing the strategy to contain the COVID-19 pandemic.Here,we determined the viral dynamics and antibody responses among 143 asymptomatic individuals identified in a massive screening of more than 5 million people in eight districts of Wuhan in May 2020.Asymptomatic individuals were admitted to the government-designated centralized sites in accordance with policy.The incidence rate of asymptomatic infection is〜2.92/100,000.These individuals had low viral copy numbers(peaked at 315 copies/mL)and short-lived antibody responses with the estimated diminish time of 69 days.The antibody responses in individuals with persistent SARS-CoV-2 infection is much longer with the estimated diminish time of 257 days.These results imply that the immune responses in the asymptomatic individuals are not potent enough for preventing SARS-CoV-2 re-infection,which has recently been reported in recovered COVID-19 patients.This casts doubt on the efficacy of forming"herd-immunity"through natural SARS-CoV-2 infection and urges for the development of safe and effective vaccines.

关键词： asymptomatic admitted immunity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

柔性蒙皮变体机翼多电机分布式驱动系统研究

机械科学与技术 2023年第9期42卷 1551-1558页

作者：随涛徐志伟南京航空航天大学机械结构力学与控制国家重点实验室南京210016

针对可变弯度柔性波纹蒙皮后缘模型结构,采用了多电机驱动技术,设计并制备了机翼模型、驱动及测控系统;采用改进的偏差耦合算法,实现了驱动系统中3台电机的角度同步控制;完成了系统软、硬件的设计,并对后缘结构模型驱动系统进行了空载... 详细信息

针对可变弯度柔性波纹蒙皮后缘模型结构,采用了多电机驱动技术,设计并制备了机翼模型、驱动及测控系统;采用改进的偏差耦合算法,实现了驱动系统中3台电机的角度同步控制;完成了系统软、硬件的设计,并对后缘结构模型驱动系统进行了空载、地面加载及风洞等多组实验研究,结果表明:测控系统可以实时控制电机间的角度偏差在允许的误差范围内,在有无载荷情况下驱动系统工作无显著差异;承受风洞载荷情况下,柔性后缘结构偏转速度可达1.7°/s,超调量小于0.3°,电机启动阶段角度差小于20°,匀速运行阶段角度差小于10°。

关键词：自适应机翼变弯度机翼多电机驱动系统偏差耦合控制

维普期刊数据库