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Surface electrocardiogram and action potential in mice lacking urea transporter UT-B

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Science China(Life Sciences) 2009年第5期52卷 474-478页

作者： MENG Yan1, ZHAO ChunYan2, ZHANG XueXin2, ZHAO HuaShan1, GUO LiRong1, Lü Bin1, ZHAO XueJian1& YANG BaoXue1,3 1 Department of Pathophysiology, school of Basic medicine, Jilin university, Changchun 130021, China 2 Department of Physiology, school of Basic medicine, Jilin university, Changchun 130021, China 3 Department of medicine, university of california, San Francisco CA 94143, USA Department of Pathophysiology School of Basic Medicine Jilin University Changchun 130021 China Department of Physiology School of Basic Medicine Jilin University Changchun 130021 China Department of medicine University of California San Francisco CA 94143 USA

UT-B is a urea transporter protein expressed in the kidney and in many non-renal tissues including erythrocytes, brain, heart, bladder and the testis. The objective of this study was to determine the phenotype of UT-B deletion in the heart. UT-B expression in the heart was studied in wild-type mice vs UT-B null mice by utilizing RT-PCR and Western blot. A surface electrocardiogram (ECG) recording (lead II) was measured in wild-type mice and UT-B null mice at the ages of 6, 16 and 52 weeks. For the action potential recording, the ventricular myocytes of 16 w mice were isolated and recorded by floating microelectrode method. The sodium current was recorded by the patch clamp technique. RT-PCR and Western blot showed the UT-B expression in the heart of wild-type mice. No UT-B transcript and protein was found in UT-B null mice. The ECG recording showed that the P-R interval was significantly prolonged in UT-B null mice ((43.5 ± 4.2), (45.5 ± 6.9) and (43.8 ± 7.6) ms at ages of 6, 16 and 52 weeks) vs wild-type mice ((38.6 ± 2.9), (38.7 ± 5.6) and (38.2 ± 7.3) ms, P<0.05). The atrial ventricular heart block type II and III only appeared in the aging UT-B null mice (52 w old). The amplitude of action potential and Vmax decreased significantly in UT-B null mice ((92.17 ± 10.56) and (101.89 ± 9.54) mV/s) vs those in wild-type mice (vs (110.51 ± 10.38) and (109.53 ± 10.64) mV/s, P<0.05). The action potential duration at 50% and 90% (APD50 and APD90) was significantly prolonged in UT-B null mice ((123.83 ± 11.17) and (195.43 ± 16.41) ms) vs that in wild-type mice ((108.27 ± 10.85) and (171.00 ± 15.53) ms, P<0.05). The maximal sodium current decreased significantly in UT-B null mice (-8.80 ± 0.92) nA vs that in wild-type mice ((-5.98 ± 1.07) nA, P<0.05). These results provide the first evidence that UT-B deletion causes progressive heart block in mice.

关键词： urea transporter heart block electrocardiogram

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Histone methyltransferase NSD2 mediates the survival and invasion of triple-negative breast cancer cells via stimulating ADAM9-EGFR-AKT signaling

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Acta Pharmacologica Sinica 2019年第8期40卷 1067-1075页

作者： Jun-Jian Wang June X.Zou Hong Wang Zhi-Jian Duan Hai-Bin Wang Peng Chen Pei-Qing Liu Jian-Zhen Xu Hong-Wu Chen school of Pharmaceutical Sciences Sun Yat-Sen University Department of Biochemistry and Molecular medicine School of MedicineUniversity of California First Affiliated Hospital of Guangzhou university of Chinese medicine Computational Systems Biology Lab Shantou University Medical College Comprehensive Cancer Center University of CaliforniaDavisSchool of Medicine

Triple-negative breast cancer(TNBC)is a heterogeneous disease with a poor prognosis due to the lack of an effective targeted *** lysine methyltransferases(KMTs)have emerged as attractive drug targets for cancer ***,the function of the majority of KMTs in TNBC has remained largely *** the current study,we found that KMT nuclear receptor binding SET domain protein 2(NSD2)is overexpressed in TNBC tumors and that its overexpression is associated with poor survival of TNBC ***2 regulates TNBC cell survival and invasion and is required for tumorigenesis and tumor ***,NSD2 directly controls the expression of EGFR and ADAM9,a member of the ADAM(a disintegrin and metalloproteinase)family that mediates the release of growth factors,such as *** its methylase activity,NSD2 overexpression stimulates EGFR-AKT signaling and promotes TNBC cell resistance to the EGFR inhibitor ***,our results identify NSD2 as a major epigenetic regulator in TNBC and provide a rationale for targeting NSD2 alone or in combination with EGFR inhibitors as a targeted therapy for TNBC.

关键词： NSD2 Cell survival Cell invasion Histone methyltransferase Triple-negative breast cancer EGFR

CT Perfusion For Ischemic Stroke

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CT Perfusion For Ischemic Stroke

中华医学会第十八次全国放射学学术会议

作者： Yanle Zhao Keck school of medicine,university of South california,USA

CT is an established tool for the diagnosis of ischemic *** CT can exclude hemorrhage and detect early sighs of infarction but cannot reliably demonstrate irreversible damaged brain tissue in the hyperacute stage of ischemic *** evaluation of patients with ischemic stroke must also include perfusion CT and CT angiography for a satisfactory imaging *** plays a key role in current

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MACROPHAGE-MEDIATED TUMOR CYTOTOXICITY IS UPREGULATED BY CISPLATINUM (CDDP)

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西南医科大学学报 1991年第4期 307-308页

作者： Gan Xiaohu et al Department of Microbiology and Immunology, UCLA school of medicine,university of california at Los Angeles

Recent studies in our laboratory have demonstrated that drug mediated cytotoxicity against tumor cells can be potentiated by recombinant TNF-α. Since TNF-α is a factor secreted by activated monocytes, it is possible that cytotoxic drugs which activate monocytes to secrete TNF-α may augment their cytotoxicity in vivo. Thus we examined the effect of a widely used chemotherapeutic drug CDDP on human blood monocytes (PBM) mediated cytotoxicity against U937 and A2780(ovarian carcinoma)tumor target cells and TNF-α secretion. The cell mediated cytotoxicity to these cell lines was determined by an 18h Cr release assay. An augmented effect was observed

关键词： CDDP TNF MACROPHAGE-MEDIATED TUMOR CYTOTOXICITY IS UPREGULATED BY CISPLATINUM PBM

Galectins and allergic inflammation

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Galectins and allergic inflammation

中华医学会第十二次全国皮肤性病学术会议

作者： Fu-Tong Liu Department of Dermatology, university of california, Davis, school of medicine, Sacramento, CA 95817

Galectins are -galactoside-binding animal lectins;presently fifteen members have been identified in mammals. Members are defined by shared consensus amino acid sequences in the carbohydrate-recognition domain (CRD) and affinity for -galactosides. The family can be subdivided into the one-CRD type, which are monomers or homodimers of one CRD;the two-CRD type, which contain two distinct but homologous CRDs in a single polypeptide chain. Among the one-CRD type, galectin-3 is unique in that it contains a nonlectin part made of proline-, glycine-rich short tandem repeats connected to a CRD.

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Heat activation of TRP channels

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Heat activation of TRP channels

第九届全国钙信号和细胞功能研讨会

作者： Jie Zheng university of california Davis school of medicine,Department of Physiology and Membrane Biology,Davis CA 95616,USA

Selected Ca-permeable TRP channels serve as cellular sensors for temperature and *** of Cathrough activated TRP channels leads to membrane excitation and triggers intracellular *** of TRPV 1-4 channels is exquisitely heat-sensitive,due to large and balanced changes in entropy and enthalpy. Identification of channel structures underlying these changes is a prerequisite for understanding the molecular mechanism of heat activation and its physiological effects. We approach this goal through a combination of *** use improved heating methods(e.g.,infrared laser)to accurately control temperature-dependent activation,

关键词： ion channel temperature sensing gating pain

Exploring the common pathogenesis pathways in autoimmune diseases by integrate data and literatures from genetics,genomics and epigenetic modification of autoimmunity

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Exploring the common pathogenesis pathways in autoimmune dis...

The 8th International Congress on SLE

作者： Hai-Tao Yang Department of medicine Division of Rheumatology & Immunology Keck school of medicine university of Southern california 2011 Zonal Ave.HMR Room#704 Los Angeles california 90033 USA

＜正＞Identification of common determinants that underlie pathogenesis of autoimmune diseases will open a window onto the pathways that lead to develop new strategies for diseases *** progress of autoimmune diseases r...

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环硅酸锆钠散治疗慢性心力衰竭患者高钾血症的临床效果观察

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心电与循环 2024年第1期43卷 59-62,66页

作者：刘波程志群俞海峰湖州市中心医院(浙江大学医学院附属湖州医院)肾内科 313000 湖州市中心医院(浙江大学医学院附属湖州医院)心内科 313000

目的观察环硅酸锆钠散对慢性心力衰竭患者高钾血症的治疗效果及安全性。方法选择2021年11月至2022年11月在湖州市中心医院接受环硅酸锆钠散治疗高钾血症的慢性心力衰竭患者42例,其中男24例,女18例,年龄45~85(65.0±8.9)岁。所有患者均接... 详细信息

目的观察环硅酸锆钠散对慢性心力衰竭患者高钾血症的治疗效果及安全性。方法选择2021年11月至2022年11月在湖州市中心医院接受环硅酸锆钠散治疗高钾血症的慢性心力衰竭患者42例,其中男24例,女18例,年龄45~85(65.0±8.9)岁。所有患者均接受10g、3次/d的硅酸锆钠散治疗,分析药物降钾效果及不良反应。结果环硅酸锆钠散治疗2 h后,血钾水平由5.68(5.46,5.85)mmol/L降至5.11(4.97,5.24)mmol/L,下降0.60(0.49,0.72)mmol/L。用药24h后血钾水平为4.34(4.03,4.50)mmol/L,下降1.35(1.24,1.62)mmol/L。与治疗前相比,治疗2、24h后血钾水平均明显降低(均P<0.05)。用药24h后发生低钾血症8例(19.0%),根据治疗后血钾水平分为低钾血症组、非低钾血症组,两组患者治疗前血钾水平[5.47(5.35,5.62)mmol/L比5.78(5.50,5.90)mmol/L]比较差异有统计学意义(Z=2.499,P=0.011)。所有患者未见其他药物相关不良反应。结论环硅酸锆钠散可在短时间内有效降低慢性心力衰竭合并的高血钾。轻度高钾血症患者治疗时有发生低钾血症风险。

关键词：慢性心力衰竭高钾血症环硅酸锆钠散

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Fluorescence resonance energy transfer analysis of subunit assembly of the ASIC channel

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Fluorescence resonance energy transfer analysis of subunit a...

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Ying GAO~1,Shuang-Shuang LIU~1,Shuang QIU~1,Wei CHENG~2,Jie ZHENG~2,Jian-Hong LUO~(1*) 1Department of Neurobiology,Institute for Neuroscience,Zhejiang university school of medicine,Hangzhou 310058, China 2 Department of Physiology and Membrane Biology,university of california,Davis,school of medicine,Davis, CA 95616,USA

＜正＞Acid-sensing ion channels（ASICs） are believed to be homo- or heteromeric complexes,which have been verified by classical methods such as co-immunoprecipitation or electrophysiological ***,the exact subunit combinations of ASICs in living cells have not been established ***,we apply assays based on fluorescence resonance energy transfer（FRET） between GFP color mutants CFP and YFP to investigate ASIC assembly directly in living *** as well as heteromerization of different combinations of ASIC subunits were *** addition,our results suggest the formation of heteromeric 1a/2a channels of stoichiometry consisting of at least two la subunits and two 2a *** stoichiometry was observed from heteromeric 1a/2b and 2a/2b *** results imply that these heteromeric ASIC channels contain at least four subunits.

关键词： FRET ASIC assembly homomer heteromer stoichiometry CHO