Fluorescence resonance energy transfer analysis of subunit assembly of the ASIC channel

作     者：Ying GAO~1,Shuang-Shuang LIU~1,Shuang QIU~1,Wei CHENG~2,Jie ZHENG~2,Jian-Hong LUO~(1*) 1Department of Neurobiology,Institute for Neuroscience,Zhejiang University School of Medicine,Hangzhou 310058, China 2 Department of Physiology and Membrane Biology,University of California,Davis,School of Medicine,Davis, CA 95616,USA 

会议名称：《中国神经科学学会第四次会员代表大会暨第七届全国学术会议》

会议日期：2007年

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

关 键 词：FRET ASIC assembly homomer heteromer stoichiometry CHO 

摘      要：正Acid-sensing ion channels（ASICs） are believed to be homo- or heteromeric complexes,which have been verified by classical methods such as co-immunoprecipitation or electrophysiological ***,the exact subunit combinations of ASICs in living cells have not been established ***,we apply assays based on fluorescence resonance energy transfer（FRET） between GFP color mutants CFP and YFP to investigate ASIC assembly directly in living *** as well as heteromerization of different combinations of ASIC subunits were *** addition,our results suggest the formation of heteromeric 1a/2a channels of stoichiometry consisting of at least two la subunits and two 2a *** stoichiometry was observed from heteromeric 1a/2b and 2a/2b *** results imply that these heteromeric ASIC channels contain at least four subunits.