检索结果-南通市图书馆

Integrated gradient tissue-engineered osteochondral scaffolds:Challenges,current efforts and future perspectives

Bioactive Materials 2023年第2期20卷 574-597页

作者： Xiaolian Niu Ning Li zhipo du Xiaoming Li Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education Beijing Advanced Innovation Center for Biomedical EngineeringSchool of Biological Science and Medical EngineeringBeihang UniversityBeijing100083China Department of Orthopedics The Fourth Central Hospital of Baoding CityBaoding072350China

The osteochondral defect repair has been most extensively studied due to the rising demand for new therapies to diseases such as osteoarthritis.Tissue engineering has been proposed as a promising strategy to meet the demand of simultaneous regeneration of both cartilage and subchondral bone by constructing integrated gradient tissue-engineered osteochondral scaffold(IGTEOS).This review brought forward the main challenges of establishing a satisfactory IGTEOS from the perspectives of the complexity of physiology and microenvironment of osteochondral tissue,and the limitations of obtaining the desired and required scaffold.Then,we comprehensively discussed and summarized the current tissue-engineered efforts to resolve the above challenges,including architecture strategies,fabrication techniques and in vitro/in vivo evaluation methods of the IGTEOS.Especially,we highlighted the advantages and limitations of various fabrication techniques of IGTEOS,and common cases of IGTEOS application.Finally,based on the above challenges and current research progress,we analyzed in details the future perspectives of tissue-engineered osteochondral construct,so as to achieve the perfect reconstruction of the cartilaginous and osseous layers of osteochondral tissue simultaneously.This comprehensive and instructive review could provide deep insights into our current understanding of IGTEOS.

关键词： Osteochondral tissue engineering Integrated gradient tissue-engineered osteochondral scaffold(IGTEOS) Tissue-engineered strategies Fabrication techniques Evaluation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fabrication and biological evaluation of polyether ether ketone(PEEK)/bioceramic composites

引用

Progress in Natural Science:Materials International 2022年第3期32卷 334-339页

作者： Junfeng Chen Guangxiu Cao Ying Li Ning Li zhipo du Xiaoming Li Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education Beijing Advanced Innovation Center for Biomedical Engineering School of Biological Science and Medical Engineering Beihang University Department of Orthopedics The Fourth Central Hospital of Baoding City

The repair of vascularized bone defects represents a significantly clinical challenge, and vascular regeneration is one of the necessary factors to promote bone tissue regeneration. To effectively repair large bone defects, new bone tissue must regenerate with a rich vascular network. Therefore, the development of biomaterials that can promote the regeneration of vascularized bone tissue is currently receiving attention from researchers. In this study, Li–Ca–Si bioceramics(LCS) containing Li, Ca, and Si elements was developed, then LCS was compounded with PEEK to prepare PEEK+10% LCS, PEEK+20% LCS, PEEK+30% LCS, and the effect of LCS-PEEK composite biomaterials on the proliferation and angiogenic ability of human umbilical vascular endothelial cells(HUVECs)further explored by Cell Counting Kit-8(CCK-8), scanning electron microscope(SEM), quantitative real-time PCR(QPCR), Western Blotting and enzyme linked immunosorbent assay(ELISA). The results showed that HUVECs inoculated on 30%LCS + PEEK material exhibited the best proliferation ability. And the adhesion ability of endothelial cells on PEEK gradually increased with the increase of LCS contents. Furthermore, the angiogenic ability of HUVECs on LCS-PEEK composites was examined using QPCR and Western blotting, and the results showed that the expression of angiogenic-related genes and proteins of HUVECs on PEEK composites gradually increased with increasing LCS concentration. These results demonstrated that the angiogenic ability of HUVECs was effectively stimulated by LCS-modified PEEK materials. The present results indicate that the PEEK material can be modified with bioceramics to promote angiogenesis, and this study lay the foundation for the subsequent development of scaffolds that promote vascularized bone tissue regeneration.

关键词： Polyether ether ketone(PEEK) Li–Ca–Si bioceramics (LCS) Human umbilical vascular endothelial cells (HUVECs) Angiogenesis Bone tissue engineering

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The effect of carbon nanotubes on osteogenic functions of adipose-derived mesenchymal stem cells in vitro and bone formation in vivo compared with that of nano-hydroxyapatite and the possible mechanism

引用

Bioactive Materials 2021年第2期6卷 333-345页

作者： zhipo du Xinxing Feng Guangxiu Cao Zhending She Rongwei Tan Katerina E.Aifantis Ruihong Zhang Xiaoming Li Department of Orthopedics The Fourth Central Hospital of Baoding CityBaoding072350China Endocrinology and Cardiovascular Disease Centre Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100037China Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education School of Biological Science and Medical EngineeringBeihang UniversityBeijing100083China Beijing Advanced Innovation Center for Biomedical Engineering Beihang UniversityBeijing100083China Guangdong Engineering Research Center of Implantable Medical Polymer Shenzhen Lando Biomaterials Co.Ltd.Shenzhen518107China Department of Mechanical and Aerospace Engineering University of FloridaGainesvilleFL32611USA Department of Research and Teaching The Fourth Central Hospital of Baoding CityBaoding072350China

It has been well recognized that the development and use of artificial materials with high osteogenic ability is one of the most promising means to replace bone grafting that has exhibited various negative effects.The biomimetic features and unique physiochemical properties of nanomaterials play important roles in stimulating cellular functions and guiding tissue regeneration.But efficacy degree of some nanomaterials to promote specific tissue formation is still not clear.We hereby comparatively studied the osteogenic ability of our treated multiwalled carbon nanotubes(MCNTs)and the main inorganic mineral component of natural bone,nano-hydroxyapatite(nHA)in the same system,and tried to tell the related mechanism.In vitro culture of human adiposederived mesenchymal stem cells(HASCs)on the MCNTs and nHA demonstrated that although there was no significant difference in the cell adhesion amount between on the MCNTs and nHA,the cell attachment strength and proliferation on the MCNTs were better.Most importantly,the MCNTs could induce osteogenic differentiation of the HASCs better than the nHA,the possible mechanism of which was found to be that the MCNTs could activate Notch involved signaling pathways by concentrating more proteins,including specific bone-inducing ones.Moreover,the MCNTs could induce ectopic bone formation in vivo while the nHA could not,which might be because MCNTs could stimulate inducible cells in tissues to form inductive bone better than nHA by concentrating more proteins including specific bone-inducing ones secreted from M2 macrophages.Therefore,MCNTs might be more effective materials for accelerating bone formation even than nHA.

关键词： Multi-walled carbon nanotubes(MCNTs) Bone repair material Protein adsorption Osteogenic differentiation Bone formation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Microstructure and Mechanical Properties of Ti–Ta Composites Prepared Through Cold Spray Additive Manufacturing

引用

Acta Metallurgica Sinica(English Letters) 2022年第9期35卷 1465-1476页

作者： Junrong Tang Naeem ul Haq Tariq zhipo Zhao Mingxiao Guo Hanhui Liu Yupeng Ren Xinyu Cui Yanfang Shen Jiqiang Wang Tianying Xiong Shi-changxu Innovation Center for Advanced Materials Institute of Metal ResearchChinese Academy of SciencesShenyang110016China School of Materials Science and Engineering University of Science and Technology of ChinaShenyang110016China Department of Metallurgy and Materials Engineering Pakistan Institute of Engineering and Applied SciencesNiloreIslamabadPakistan

In this study, an innovative approach was used to fabricate Ti–Ta composite biomaterials through cold spray additive manufacturing followed by a diff usion treatment. The microstructure and mechanical properties of the composites were investigated in detail using field emission scanning electron microscopy, electron backscatter diff raction, 3D X-ray computed tomography, tensile test, nanohardness test and resonance vibration test. The obtained results indicated that the prepared composites have inhomogeneity in their microstructure and composition. A unique microstructure, composed of Ti-rich, Ta-rich and diff usion regions, was evolved in the composites due to incomplete diff usion between Ti and Ta splats. Further, Kirkendall pores were formed in the composites due to uneven diff usion of the two phases(of Ti and Ta) during high-temperature heat treatment. The prepared composites simultaneously showed low elastic modulus and high tensile strength which is required for a good biomaterial. Low elastic modulus was associated with the residual pores and the alloying eff ect of Ta in Ti, while high tensile strength was related to the solid solution strengthening eff ects. The obtained results indicated that the prepared Ti–Ta composites have a great potential to become a new candidate for biomedical applications.

关键词： Ti–Ta composites Cold spray additive manufacturing(CSAM) Biomaterials Mechanical properties X-ray computed tomography(XCT)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Microstructure and bioactivity of a cold sprayed rough/porous Ta coating on Ti6Al4V substrate

引用

Science China(Technological Sciences) 2020年第5期63卷 731-739页

作者： TANG JunRong ZHAO zhipo CUI XinYu WANG JiQiang XIONG TianYing Institute of Metal Research Chinese Academy of SciencesShenyang 110016China School of Materials Science and Engineering University of Science and Technology of ChinaShenyang 110016China

To improve the bioactivity and biocompatibility of titanium implants,a rough/porous tantalum(Ta)coating was firstly prepared on Ti6Al4V substrate by cold spraying.The results indicated that the surface of Ta coating is extremely rough with lots of visible holes,because of poor deposition quality.Microstructure and microhardness results showed that different layers appeared in the inner and outer parts of coatings,corresponding to porosity,due to lack of subsequent compaction.The simulated body fluid(SBF)soaking test showed that spherical apatite sediments were mineralized on rough/porous surface in SBF after 2–4 weeks,which demonstrated that the cold sprayed Ta coating had good bioactivity.This was mainly attributed to the rough/porous surface of Ta coating obtained by cold spraying,which is conducive to the heterogeneous nucleation of apatite on it.

关键词： cold spray tantalum coatings SBF soaking test bioactivity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

ChatGPT类生成式人工智能的法律风险及治理(笔谈)

引用

天津师范大学学报（社会科学版） 2024年第3期 21-38页

作者：王新王宣亿黄晓亮赵毅沈维清谭波赵智张志坡朱沛智北京大学法学院北京100871 北京师范大学法学院北京100875 苏州大学王健法学院苏州215000 海南大学党内法规研究中心海口570028 海南大学法学院南开大学法学院天津300350 天津师范大学法学院天津300387

以ChatGPT(Chat Generative Pre-trained Transformer)为代表的生成式人工智能技术自诞生以来,引发了社会各领域深度变革,生成式人工智能为大数据时代提供了高智能性分析工具和技术应用,但同时亦引发了关于数据安全、技术伦理、知识产... 详细信息

以ChatGPT(Chat Generative Pre-trained Transformer)为代表的生成式人工智能技术自诞生以来,引发了社会各领域深度变革,生成式人工智能为大数据时代提供了高智能性分析工具和技术应用,但同时亦引发了关于数据安全、技术伦理、知识产权保护等争端。为保障生成式人工智能的健康发展和规范应用,我国近期先后出台了《生成式人工智能服务管理暂行办法》《新一代人工智能伦理规范》等法律法规,这对引导我国生成式人工智能的科学发展具有基础性保障作用和重要指导意义。为深化生成式人工智能的理论研究,全面解构其存在的法律风险并探求解决路径,本刊以“ChatGPT类生成式人工智能的法律风险及治理”为题发表系列笔谈文章。专家们从数据安全、虚假信息规制、知识产权保护等多维度,以不同法学学科的多视角系统论证了以ChatGPT为代表的生成式人工智能存在的法律风险,并针对性提出解决方案及完善路径。

关键词：知识产权保护完善路径暂行办法法学学科人工智能信息规制数据安全笔谈文章

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

DCE-MRI参数联合IFIT1基因对腰椎间盘突出患者椎间盘退变程度的评估价值

引用

影像科学与光化学 2022年第1期40卷 11-16页

作者：杜志坡马淑芳李警王建波张朝崔坤坤保定市第四中心医院河北保定072350

探讨DCE-MRI参数及IFIT1基因对腰椎间盘突出症(LDH)患者椎间盘退变(IDD)程度的评估价值。随机选取确诊的66例LDH患者作为研究对象,根据改良Pfirrmann分级法将患者分为2组:轻中度IDD(Ⅰ~Ⅲ级)组(46例)和重度IDD(Ⅳ~Ⅴ级)组20例。通过DCE-... 详细信息

探讨DCE-MRI参数及IFIT1基因对腰椎间盘突出症(LDH)患者椎间盘退变(IDD)程度的评估价值。随机选取确诊的66例LDH患者作为研究对象,根据改良Pfirrmann分级法将患者分为2组:轻中度IDD(Ⅰ~Ⅲ级)组(46例)和重度IDD(Ⅳ~Ⅴ级)组20例。通过DCE-MRI检查考察患者的K-trans值,采用日本骨科学会评分(JOA)评估腰椎功能,采用视觉模拟评分法(VAS)评估腰部疼痛,采用RT-PCR检测外周血单个核细胞(PBMC)中IFIT1的mRNA表达。轻中度IDD组的K-trans值显著低于重度IDD组。轻中度IDD组患者PBMC中的IFIT1 mRNA相对表达量显著低于重度IDD组。K-trans值与PBMC中的IFIT1 mRNA水平和VAS评分正相关,与JOA评分负相关(P<0.05)。PBMC中的IFIT1 mRNA水平与VAS评分正相关,与JOA评分负相关(P<0.05)。K-trans值和PBMC中的IFIT1 mRNA水平联合诊断重度IDD的AUC值、敏感性和特异性分别为0.991、91.3%和100.0%,AUC值和特异性均高于单独诊断。DCE-MRI检查中的K-trans值联合PBMC中的IFIT1 mRNA水平可用于LDH患者IDD程度的诊断。

关键词：动态对比增强磁共振成像参数 IFIT1基因腰椎间盘突出症椎间盘退变日本骨科学会评分视觉模拟评分法

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

南水北调工程彰显综合效益打造天津高质量发展引擎

引用

水利发展研究 2022年第5期22卷 6-9页

作者：张志颇天津市水务局天津300074

天津是资源型缺水的特大城市,南水北调中线工程是继引滦入津工程之后的又一条城市供水生命线,为保障天津城市供水安全发挥了重要作用。在此基础上,南水北调来水极大地完善了天津的供水格局,带动了天津水生态环境的整体改善,催生出更加... 详细信息

天津是资源型缺水的特大城市,南水北调中线工程是继引滦入津工程之后的又一条城市供水生命线,为保障天津城市供水安全发挥了重要作用。在此基础上,南水北调来水极大地完善了天津的供水格局,带动了天津水生态环境的整体改善,催生出更加符合高质量发展需求的水资源管理体系,显现出巨大的综合效益,促进了天津经济社会高质量发展,更为今后推动南水北调后续工程高质量发展,更好地发挥引江供水效益积累了宝贵经验。

关键词：南水北调工程综合效益城市供水天津市

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

如何构建独立、有效的监事会?

引用

经贸法律评论 2022年第2期 68-88页

作者：张志坡南开大学法学院

《公司法》规定的监事会制度存在缺陷,实践中监督效果不理想,监事会制度需要重构。监事会的功能发挥取决于监事独立性、监督可能性和监督有效性。改革监事的选任和解任规则,可以实现监事独立性。提升监事能力、扩大监事职权、保护职工... 详细信息

《公司法》规定的监事会制度存在缺陷,实践中监督效果不理想,监事会制度需要重构。监事会的功能发挥取决于监事独立性、监督可能性和监督有效性。改革监事的选任和解任规则,可以实现监事独立性。提升监事能力、扩大监事职权、保护职工监事权益可以确保监督可能性。强化监事会组织、改革监事行使职权方式、明确监事义务和责任、营造良好公司文化等可以提高监督有效性。有效的监事会治理将提升中国公司的竞争力。

关键词：公司治理监事会独立性监事职权有效性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

动物的著作权?——兼论人工智能生成物的性质与归属

引用

辽宁师范大学学报（社会科学版） 2022年第4期45卷 54-60页

作者：张志坡南开大学法学院天津300350

法律主体具有伦理性特征。动物“创作”成果仅具有作品的形式,并不符合作品的构成要件。动物与人相似,具有一定的意志,但其不是法律主体,不是著作权主体。动物“创作”成果的出版或利用以权属明确为前提。动物“创作”成果的归属应考虑... 详细信息

法律主体具有伦理性特征。动物“创作”成果仅具有作品的形式,并不符合作品的构成要件。动物与人相似,具有一定的意志,但其不是法律主体,不是著作权主体。动物“创作”成果的出版或利用以权属明确为前提。动物“创作”成果的归属应考虑到人与动物关系的复杂性,单纯归投资人享有并不妥当。根据法律和法理,动物“创作”成果的归属应分为三种类型加以处理才能较好地实现利益平衡。人工智能同样并非法律主体,当其构成人创作的工具时,其生成物可能构成作品,适用著作权规则;其他情形则不产生著作权,其利益归属适用孳息规则。

关键词：动物“创作” 人工智能生成物作品著作权利益归属

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：