The effect of carbon nanotubes on osteogenic functions of adipose-derived mesenchymal stem cells in vitro and bone formation in vivo compared with that of nano-hydroxyapatite and the possible mechanism

作     者：Zhipo Du Xinxing Feng Guangxiu Cao Zhending She Rongwei Tan Katerina E.Aifantis Ruihong Zhang Xiaoming Li 

作者机构：Department of OrthopedicsThe Fourth Central Hospital of Baoding CityBaoding072350China Endocrinology and Cardiovascular Disease CentreFuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100037China Key Laboratory for Biomechanics and Mechanobiology of Ministry of EducationSchool of Biological Science and Medical EngineeringBeihang UniversityBeijing100083China Beijing Advanced Innovation Center for Biomedical EngineeringBeihang UniversityBeijing100083China Guangdong Engineering Research Center of Implantable Medical PolymerShenzhen Lando Biomaterials Co.Ltd.Shenzhen518107China Department of Mechanical and Aerospace EngineeringUniversity of FloridaGainesvilleFL32611USA Department of Research and TeachingThe Fourth Central Hospital of Baoding CityBaoding072350China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2021年第6卷第2期

页      面：333-345页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 07[理学] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0836[工学-生物工程] 0702[理学-物理学] 

基　　金：The authors acknowledge the financial supports from the National Natural Science Foundation of China(No.31771042) Fok Ying Tung Education Foundation(No.141039) State Key Laboratory of New Ceramic and Fine Processing Tsinghua University,Fund of Key Laboratory of Advanced Materials of Ministry of Education(No.2020AML10) International Joint Research Center of Aerospace Biotechnology and Medical Engineering,Ministry of Science and Technology of China,and the 111 Project(No.B13003) 

主　　题：Multi-walled carbon nanotubes(MCNTs) Bone repair material Protein adsorption Osteogenic differentiation Bone formation 

摘      要：It has been well recognized that the development and use of artificial materials with high osteogenic ability is one of the most promising means to replace bone grafting that has exhibited various negative *** biomimetic features and unique physiochemical properties of nanomaterials play important roles in stimulating cellular functions and guiding tissue *** efficacy degree of some nanomaterials to promote specific tissue formation is still not *** hereby comparatively studied the osteogenic ability of our treated multiwalled carbon nanotubes(MCNTs)and the main inorganic mineral component of natural bone,nano-hydroxyapatite(nHA)in the same system,and tried to tell the related *** vitro culture of human adiposederived mesenchymal stem cells(HASCs)on the MCNTs and nHA demonstrated that although there was no significant difference in the cell adhesion amount between on the MCNTs and nHA,the cell attachment strength and proliferation on the MCNTs were *** importantly,the MCNTs could induce osteogenic differentiation of the HASCs better than the nHA,the possible mechanism of which was found to be that the MCNTs could activate Notch involved signaling pathways by concentrating more proteins,including specific bone-inducing ***,the MCNTs could induce ectopic bone formation in vivo while the nHA could not,which might be because MCNTs could stimulate inducible cells in tissues to form inductive bone better than nHA by concentrating more proteins including specific bone-inducing ones secreted from M2 ***,MCNTs might be more effective materials for accelerating bone formation even than nHA.