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Two Retroviruses Packaged in One Cell Line can Combined Inhibit the Replication of HIV-1 in TZM-bl Cells

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Virologica Sinica 2012年第6期27卷 339-344页

作者： zhipin.Liang Zhiyuan Guo Xin wang Xiaohong Kong Chang Liul School of Medicine Nankai University Tianjin 300071 China Tianjin Centers for Disease Control and Prevention Tianjin 300171 China

The cellular protein tetherin tethers the HIV-1 viral particles on the cellular membrane to inhibit the replication of HIV-1. However, the HIV-1 accessory protein Vpu counteracts the antiviral function of tetherin. In this study, two retroviral vector plasmids were constructed. One inhibited the vpu gene expression; the other one over-expressed the tetherin. Both retroviral vector plasmids could be packaged in the packaging cell line PT67 to obtain the corresponding retroviruses. The retroviral vector plasmids＇ functions of tetherin over-expression or vpu-RNAi were detected at the cell level. Retroviral vector plasmids were transfected to PT67 cells at different ratios from 0T3V to 3TOV, and then mixed retroviruses were harvested. The antiviral functions of mixed retroviruses were detected in HIV-1 infected TZM-bi cells. The results showed that packaged mixed retroviruses could repress the replication of HIV-1 in TZM-bl cells.

关键词： HIV- 1 Vpu Tetherin Gene therapy

FREPD: A Robust Federated Learning Framework on Variational Autoencoder

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Computer Systems Science & Engineering 2021年第12期39卷 307-320页

作者： zhipin.Gu Liangzhong He Peiyan Li Peng Sun Jiangyong Shi Yuexiang Yang National University of Defense Technology Changsha410000China China Mobile(Suzhou)Software Technology Co.Ltd. Suzhou215000China Eindhoven University of Technology Eindhoven5641BZNetherlands

Federated learning is an ideal solution to the limitation of not preser-ving the users’privacy information in edge *** federated learning,the cloud aggregates local model updates from the devices to generate a global *** protect devices’privacy,the cloud is designed to have no visibility into how these updates are generated,making detecting and defending malicious model updates a challenging *** existing works that struggle to tolerate adversarial attacks,the paper manages to exclude malicious updates from the glo-bal model’s *** paper focuses on Byzantine attack and backdoor attack in the federated learning *** propose a federated learning frame-work,which we call Federated Reconstruction Error Probability Distribution(FREPD).FREPD uses a VAE model to compute updates’reconstruction *** with higher reconstruction errors than the average reconstruction error are deemed as malicious updates and ***,we apply the Kolmogorov-Smirnov test to choose a proper probability distribution function and tune its parameters to fit the distribution of reconstruction errors from observed benign *** then use the distribution function to estimate the probability that an unseen reconstruction error belongs to the benign reconstruction error *** on the probability,we classify the model updates as benign or *** benign updates are used to aggregate the global *** is tested with extensive experiments on independent and identically distributed(IID)and non-IID federated benchmarks,showing a competitive performance over existing aggregation methods under Byzantine attack and backdoor attack.

关键词： Federated learning reconstruction error probability distribution

The Comparison of Genetic Variation in the Envelope Protein Between Various Immunodeficiency Viruses and Equine Infectious Anemia Virus

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Virologica Sinica 2012年第4期27卷 241-247页

作者： Qing Yuan Chang Liu zhipin.Liang Xueqing Chen Danhong Diao Xiaohong Kong Laboratory of Medical Molecular Virology School of MedicineNankai UniversityTianjin 300071China

The envelope protein （Env） of lentiviruses such as HIV, SIV, FIV and EIAV is larger than that of other retroviruses. The Chinese EIAV attenuated vaccine is based on Env and has helped to successfully control this virus, demonstrating that envelope is crucial for vaccine. We compared Env variation of the four kinds of lentiviruses. Phylogenetic analysis showed that the evolutionary relationship of Env between HIV and SIV was the closest and they appeared to descend from a common ancestor, and the relationship of HIV and EIAV was the furthest. EIAV had the shortest Env length and the least number of potential N-linked glyeosylation sites （PNGS） as well as glyeosylation density compared to various immunodefieiency viruses. However, HIV had the longest Env length and the most PNGS. Moreover, the alignment of HIV and SIV showed that PNGS were primarily distributed within extraeellular membrane protein gp120 rather than transmembrane gp41. It implies that the size difference among these viruses is associated with a lentivirus specific function and also the diversity of env. There arc low levels of modification of glycosylation sites of Env and selection of optimal protective epitopes might be useful for development of an effective vaccine against HIV/AIDS.

关键词： HIV Simian immunodeficiency virus (SIV) Feline immunodeficiency virus (FIV) Equine infectious anemia virus (EIAV) PotentialN-linked glycosylation sites (PNGS) Envelope protein (Env)

Cyclin G2,a novel target of sulindac to inhibit cell cycle progression in colorectal cancer

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Genes & Diseases 2021年第3期8卷 320-330页

作者： Hongyou Zhao Bin Yi zhipin.Liang Ches’Nique Phillips Hui-Yi Lin Adam I.Riker Yaguang Xi Department of Genetics and Stanley S.Scott Cancer Center Louisiana State University Health Sciences CenterNewOrleans70112USA School of Public Health Louisiana State University Health Sciences CenterNew Orleans70112USA Geaton and JoAnn DeCesaris Cancer Institute Anne Arundel Medical CenterLuminis HealthAnnapolisMD21401USA

Sulindac has shown significant clinical benefit in preventing colorectal cancer pro-gression,but its mechanism of action has not been fully *** have found that sulin-dac sulfide(SS)is able to inhibit cell cycle progression in human colorectal cancer cells,particularly through G1 *** understand the underlying mechanisms of sulindac inhibitory activity,we have demonstrated that Cyclin G2 up-regulation upon SS treatment can substan-tially delay cell cycle progression by enhancing the transcriptional activity of FOXO3a in human colorectal tumor ***-182,an oncogenic microRNA known to inhibit FOXO3a gene expres-sion,is also involved in the suppressive effect of SS on cell cycle *** process be-gins with the down-regulation of miR-182,followed by the enhancement of FOXO3a transcriptional activity and the up-regulation of Cyclin *** further determine the clinical utility of this axis,we analyzed the expression of miR-182/FOXO3a/Cyclin G2 in human colo-rectal tumor *** results show not only that there are significant dfferences in miR-182/FOXO3a/Cyclin G2 between tumors and normal tissues,but also that the synergetic effect of miR-182 and FOXO3a is associated with predicting tumor *** study dem-onstrates a novel mechanistic axis consisting of miR-182/FOXO3a/Cyclin G2 that mediates su-lindac inhibition of cell cycle progression.

关键词： Cell cycle Colorectal cancer Gene regulation miRNA Sulindac

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芍陂的兴废及原因

历史教学 2004年第8期 65-67页

作者：刘治品山东省蓬莱二中，山东蓬莱265612

春秋战国时期水利灌溉事业有很大发展,出现了许多著名的水利工程,其中有春秋时楚相孙叔敖修的芍陂.那么为什么要修建芍陂?芍陂在历史上产生过哪些作用?芍陂在历史上发生过什么变化?变化的原因是什么?我们应该总结哪些经验,吸取哪些教训?

关键词：芍陂楚国水利灌溉事业自然环境

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庄子技术论及其指向

现代哲学 2009年第3期 129-132页

作者：曹智频华南理工大学新闻与传播学院

庄子对技术问题提出了个性化的理解,分析了技术的功能表现,认为技术伤害了人类本性,希望通过技术手段和超技术方式来解决技术影响。

关键词：庄子技术论技术形式技术超越

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媒介偏向与文化变迁:从伊尼斯到麦克卢汉

学术研究 2010年第8期 129-133页

作者：曹智频华南理工大学新闻与传播学院

媒介偏向影响着人类文明的走向。媒介将信息转化为知识体系,并生成一种文化机制,引导着文化的发展路向。在诸多媒介演化中,口语媒介阶段是最圆融自在的历史时期,这与当下数字媒介的状况相类似——3G表现为逻辑上的口语回归,但其主体隐... 详细信息

媒介偏向影响着人类文明的走向。媒介将信息转化为知识体系,并生成一种文化机制,引导着文化的发展路向。在诸多媒介演化中,口语媒介阶段是最圆融自在的历史时期,这与当下数字媒介的状况相类似——3G表现为逻辑上的口语回归,但其主体隐没和经验缺失的交流情境给世界文化建设带来了诸多弊端。从文化安全视角看,回归文化传统和民族价值观能够有效规避新媒体时期的文化困境。

关键词：媒介偏向文化变迁口语文化伊尼斯麦克卢汉

论道家思想的传播与文化走向--对李锦全先生道家研究的梳理

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广东社会科学 2009年第2期 51-55页

作者：曹智频华南理工大学新闻与传播学院副教授广州510003

道家文化在几千年的传播中分化为其意义演化和功能表达,前者是一个学理化的提升过程,后者是一个生活化的过程。在与主流文化的激荡过程中,道家文化的传播逐步潜层化。

关键词：道家符号学意义功能传播

Analysis of Food Safety Supervision and Spot-Check in Sichuan Province in 2019

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Agricultural Biotechnology 2022年第2期11卷 70-74页

作者： Pengyu CHEN Wei wang Xueli WU Lili JI Jiamin ZHANG zhipin.ZHAO Rui ZHANG Ting BAI Bo HOU Yixi YANG Lin CHEN Key Lab of Meat Processing of Sichuan Province Chengdu UniversityChengdu 610106China Chengdu institute of Product Quality Inspection Co. Ltd.Chengdu 610106China

Through the analysis on 211614 batches of samples from 35 categories of food safety supervision and inspection in Sichuan Province by Administration for Market Regulation of Sichuan Province in 2019,four suggestions were finally put *** analysis results showed that in 2019,the overall trend of food safety in Sichuan Province was stable and improved.A total of 30 categories of unqualified samples were detected,and a total of 12135 batches of unqualified samples were detected,with an average sample unqualified rate of 5.73%.Among them,the main problems of unqualified samples were as follows:the use of food additives beyond the scope and limit,accounting for 40.17%of the total number of unqualified samples,microbial contamination in food,accounting for 23.61%of the total unqualified samples,the residue index of agricultural and veterinary drugs in food not meeting the standard,accounting for 15.61%of the total unqualified samples,the quality index in food not meeting the standard,accounting for 8.51%of the total number of unqualified samples,and heavy metals and other pollutants in food exceeding the standard,accounting for 7.10%of the total unqualified samples.

关键词： Sichuan Food safety Supervision and spot-check Recommendations