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MicroRNA-93 mediates cabergoline-resistance by targeting ATG7 in prolactinoma

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MicroRNA-93 mediates cabergoline-resistance by targeting ATG...

2018年浙江省神经外科学学术年会

作者： Zerui Wu Lin Cai Jianglong Lu zhipeng su First Affiliated Hospital of Wenzhou Medical University

Objective Our previous study found that expression of miRNA-93 increased obviously in DA-resistant prolactinomas, however, the role of miRNA-93 in DA-resistance was largely unexplored. This study is to find regulatory mechanism underlyingmiRNA-93 inhibits CAB *** The expression of miRNAs in human pituitary tumors was analyzed by microarray and RT-PCR. Cell viability and proliferation was determined by MTS assays.

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RBM17 controls apoptosis and proliferation to promote Glioma progression

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RBM17 controls apoptosis and proliferation to promote Glioma...

2018年浙江省神经外科学学术年会

作者： Chengde wang Jianglong Lu zhipeng su Qun Li The first affiliated hospital of wenzhou medical university

Objective The splicing factor SPF45 (RBM17) is a well-known component of the spliceosome that is involved in alternative splicing. RBM17 is frequently overexpressed in many tumors and plays a crucial role in cancer progression and drug resistance. However, the role of RBM17 in the development of glioma has not been thoroughly elucidated to date.

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Construction of lentiviral vector encoding human vascular endothelial growth factor165 gene and transfection of human neural stem cells

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Construction of lentiviral vector encoding human vascular en...

2014浙江省神经外科学学术年会

作者： Yu Zhang zhipeng su Huajun Jing Xiaobing Wang Qichuan Zhuge Department of Neurosurgery First Affiliated Hospital of Wenzhou Medical University

Background:Despite neural stem cells(NSCs)transplantation can promote function recovery of intracerebral hemorrhage(ICH)rats,low survival rate of grafted NSCs in ICH rats is a grave *** studies show that vascular endothelial growth factor(VEGF)play key roles in proliferation,differentiation and survival of ***:We construct a lentiviral vector carrying VEGF165 gene,which was used to transfect human NSCs,so as to produce therapeutic seed *** amplified VEGF165gene from human breast cancer cell line MCF-7 using RT-PCR and cloned the target gene into the pCDH-CMV-MCS-EF1-copGFP,a lentiviral expressing *** transformation,Enzyme digestion got a correct length of VEGF165 gene,DNA sequencing analysis confirmed that VEGF165 gene sequence was exactly same as that reported by *** then transfected the recombinant lentivirus produced by 293T cells after cotransfection of pCDH-VEGF165 and packaging plasmids into passage fourth human *** efficiency is 63%,tested by flow cytometry(FCM),VEGF165 mRNA and protein stable expression in transfected NSCs(VEGF165-NSCs)from 1 week after infection,and continue to 2 ***:Proliferation and VEGF secretion ability of NSCs significantly increased in pCDH-VEGF165 transfection group compared with empty vector transfected group and bland control ***-VEGF165 transfected NSCs still keep their characteristics and multiplex differentiation ***:Our results indicate that we have constructed the lentiviral vector carrying human VEGF165 gene successfully and accomplished transfection of human NSCs,which may be used as a gene therapeutic tool to promote proliferation,differentiation and survival of NSCs in treatment of ICH.

关键词： VEGF Lentiviral vector Neural stem cell(NSC) Transfection

Genome-wide association with transcriptomics reveals a shade-tolerance gene network in soybean

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The Crop Journal 2024年第1期12卷 232-243页

作者： Yanzhu su Xiaoshuai Hao Weiying Zeng Zhenguang Lai Yongpeng Pan Can Wang Pengfei Guo zhipeng Zhang Jianbo He Guangnan Xing Wubin Wang Jiaoping Zhang Zudong sun Junyi Gai Soybean Research Institute/MARA National Center for Soybean Improvement MARA Key Laboratory of Biology and Genetic Improvement of Soybean/State Key Laboratory for Crop Genetics and Germplasm Enhancement/State Innovation Platform for Integrated Production and Education in Soybean Bio-breeding/Jiangsu Collaborative Innovation Center for Modern Crop Production Nanjing Agricultural UniversityNanjing 210095JiangsuChina Guangxi Academy of Agricultural Sciences Nanning 530007GuangxiChina

Shade tolerance is essential for soybeans in inter/relay cropping systems.A genome-wide association study(GWAS)integrated with transcriptome sequencing was performed to identify genes and construct a genetic network governing the trait in a set of recombinant inbred lines derived from two soybean parents with contrasting shade *** improved GWAS procedure,restricted two-stage multi-locus genome-wide association study based on gene/allele sequence markers(GASM-RTM-GWAS),identified 140 genes and their alleles associated with shade-tolerance index(STI),146 with relative pith cell length(RCL),and nine with *** of these genes by biological categories allowed the construction of a protein–protein interaction network by 187 genes,of which half were differentially expressed under shading and non-shading conditions as well as at different growth *** the identified genes,three ones jointly identified for both traits by both GWAS and transcriptome and two genes with maximum links were chosen as beginners for entrance into the ***,both STI and RCL gene systems worked for shade-tolerance with genes interacted each other,this confirmed that shadetolerance is regulated by more than single group of interacted genes,involving multiple biological functions as a gene network.

关键词： Soybean(Glycine max(L.)Merr.) Shade-tolerance Restricted two-stage multi-locus genomewide association study based on gene/allele sequence markers(GASM-RTM-GWAS) Shade-tolerance index(STI) Relative cell length(RCL) Transcriptome

MicroRNA-326 increase the sensitivity of GH3rat pituitary adenoma cells to dopamine agonists treatment through targeting nerve growth factor receptor

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MicroRNA-326 increase the sensitivity of GH3rat pituitary ad...

2014浙江省神经外科学学术年会

作者： Lu Jianglong Cai Lin Lin Shao Jian su zhipeng Wu Zhebao Department of Neurosurgery First Affiliated Hospital of Wenzhou Medical University Department of Neurosurgery Ruijin HospitalShanghai Jiao Tong University

Prolactinoma is the most common type of pituitary adenomas and dopamine receptor agonists(DAs)are the first-line choice of ***,approximate 5%to 18%cases are DA-resistant *** of dopamine-resistant prolactinomas remains...

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Micro-source Inverters With Capacitive Equivalent Output Impedance for Improving Micro-grid Voltages

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中国电机工程学报 2013年第9期33卷 I0001-I0001页

作者： LU zhipeng LIU Haitao su Jian ZHOU Cheng WU Ming China Electric Power Research Institute

将分布式电源逆变器等效输出阻抗设计成容性可使其体现对系统无功电压调整过程相逆的电源特性,这种特性使得分布式电源逆变器更容易参与公共连接点处的电压调整。对比等效输出阻抗成感性、阻性和容性的3类逆变器下垂控制特性,对容性等... 详细信息

将分布式电源逆变器等效输出阻抗设计成容性可使其体现对系统无功电压调整过程相逆的电源特性,这种特性使得分布式电源逆变器更容易参与公共连接点处的电压调整。对比等效输出阻抗成感性、阻性和容性的3类逆变器下垂控制特性,对容性等效输出阻抗逆变器电压电流控制环进行数学建模;针对容性等效输出阻抗逆变器设计具备下垂调节和克服电压偏离功能的鲁棒功率外环控制器。对比仿真和实验证明,容性输出阻抗分布式电源逆变器控制器能够快速抑制系统功率变化带来的电压波动,能有效地参与系统电压调整。

关键词：等效输出阻抗逆变器电网电压电容公共连接点分布网络电压调节无功功率

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高速铁路无砟轨道结构注浆抬升技术的应用

铁道建筑 2021年第11期61卷 105-108页

作者：杨玺苏志鹏上海申元岩土工程有限公司上海200011

以一高速铁路路基区段无砟轨道结构不均匀沉降整治工程为背景,分析了无砟轨道结构不均匀沉降病害的特点及成因,介绍了无砟轨道结构注浆抬升技术的原理、施工工艺流程及施工质量控制要点。该技术利用改性聚酯注浆材料反应前易流动、反应... 详细信息

以一高速铁路路基区段无砟轨道结构不均匀沉降整治工程为背景,分析了无砟轨道结构不均匀沉降病害的特点及成因,介绍了无砟轨道结构注浆抬升技术的原理、施工工艺流程及施工质量控制要点。该技术利用改性聚酯注浆材料反应前易流动、反应时快速膨胀、反应后快速固结的特点,将其注入无砟轨道基床的级配碎石表层,实现对无砟轨道结构的竖向顶升。工程实践证明,无砟轨道结构注浆抬升技术能够在单个天窗内对轨道结构实现快速、同步、精确抬升,恢复线路平顺性,具有较好的应用前景。

关键词：高速铁路注浆抬升现场试验改性聚酯注浆材料不均匀沉降整治施工工艺

Effect of emodin against endoplasmic reticulum stress in rat with severe acute pancreatitis

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Effect of emodin against endoplasmic reticulum stress in rat...

全国中药药理学会联合会学术交流大会

作者： Wu Li,Cai Baochang,Liu Xiao,Cai Hao,Chen zhipeng,su Yachun,Li Huan, Guo Hui

Objective:To investigate the protective effect of emodin against endoplasmic reticulum(ER) stress in rats with severe acute pancreatitis(SAP) and its molecule mechanism. Methods:Fourty-five SD male rats were randomly divided into three groups:sham operation group (SO),SAP model group(SAP) and,emodin treat group(ET)(n=15).Each group contanis 15 SD rats was further divided into three subgroups based on the time points of constructing mode:3h,6h and 12h subgroups(n=5 each).The model of SAP was constructed through injecting sodium taurocholate into pancreatic and biliary duct in rat,emodin(3mg/100g body weight) or 0.9%NaCl was injected via the external jugular vein immediately after duct infusion of sodium taurocholate. the serum was Collected after completion of the experiment,and amylase activity in serum was measured by enzyme assay kits,tumor necrosis factor-alpha(TNF-α) and interleukin-6(IL-6) levels in serum were assayed by ELISA *** histopathology was examined with hematoxylin and eosin *** ER ultrastructure was examined by transmission electron *** of ER stress transducers Bip,IRE1αand the downstream molecules TRAF2, ASK1,JNK and p38 MAPK in pancreas were examined by western ***:Sodium taurocholate induced pancreatic injury and ER lumen dilated in exocrine pancreas in rats at 3,6 and 12 h time points,ER stress transducers Bip,IRE1αand their downstream molecules TRAF2,ASK1 in pancreatis up-regulated,further more,phosphorylation of JNK and p38MAPK in pancreas increased, which induced high expression level of inflammatory cytokine such as TNF-αand *** with emodin obviously ameliorated pancreatic injury,decreased the releasion of amylase and inflammatory *** studies showed that emodin significantly reduced the dilated ER lumen in exocrine pancreas,decreased ER stress transducers Bip,IRE1α,TRAF2,ASK1 protein expression and inhibited phosphorylation of JNK and p38 MAPK in pancreas in rats

关键词： Severe acute pancreatitis Endoplasmic reticulum stress Emodin

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数字时代雕塑材料发展的猜想

雕塑 2014年第3期 38-41页

作者：宿志鹏中国美术学院

绪论晴朗的夏季,搭乘航班往返于城市之间。夜晚,飞机徐徐下降,差不多滑翔过大半个城市,俯身端详,我相信你同我一样惊讶过。斑斓的画卷在地面上铺开,无边无垠,像崩裂的岩浆,像倒转的星河,像发光的地图,亦或更像是一组巨大的艺术装置。装... 详细信息

绪论晴朗的夏季,搭乘航班往返于城市之间。夜晚,飞机徐徐下降,差不多滑翔过大半个城市,俯身端详,我相信你同我一样惊讶过。斑斓的画卷在地面上铺开,无边无垠,像崩裂的岩浆,像倒转的星河,像发光的地图,亦或更像是一组巨大的艺术装置。装置的作者是谁？要经历怎样的步骤？我忍不住联想到爱迪生发明了电灯,造就了这一切,发明家也是伟大的艺术家,可转而一想,若没有远程输电技术,怎么会有这如此大规模空中俯瞰的效果呢;若没有庞大的城市地下管网、立交桥和汽车,谁又会去建造这庞大系统呢？是因为新技术的发明造就了这一切？还是美在心中，只要艺术的感觉在，离开了科技进步，艺术依然会发展？思绪纷繁，最终还是纠结在科技进步和艺术发展的根结上。

关键词：雕塑材料数字时代城市地下管网猜想科技进步艺术家发明家艺术发展