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Facile and diverse logic circuits based on dumbbell DNA-templated fluorescent copper nanoclusters and S1 nuclease detection

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Chinese Chemical Letters 2022年第5期33卷 2711-2714页

作者： Zefeng Gu Anchen Fu Ru Qiu Ru Sun zhijuan cao Shanghai Key Laboratory of Bioactive Small Molecules&Department of Pharmaceutical Analysis School of PharmacyFudan UniversityShanghai 201203China

DNA-based logic gates promote the development of molecular computing and show enormous potential in the fields of nanotechnology and biotechnology. Dumbbell oligonucleotides(DNA) with poly-thymine(poly-T) loops and a nicked random double strand have been demonstrated to be an efficient template for the formation of fluorescent copper nanoclusters(Cu NCs) in our previous work. Herein, a new platform technology is presented with which to construct molecular logic gates by employing Cu NCs probe as a basic output generator, coupling of functional nucleases as the inputs. Two dumbbell DNAs are used with the difference in stem length(8 bp and 16 bp, respectively). The degradation of DNA templates can be tuned by various nucleic acid enzymes, single-stranded nuclease(S1), double-stranded specific nuclease(DSN), E. coli DNA ligase, exonucleases Ⅰ and Ⅲ. Briefly, S1 can digest both DNA templates, while the cleavage ability of DSN will be resistant by the short stem of SS-DNA(short-stem DNA). Exonuclease Ⅰ and Ⅲ can degrade these two nicked DNA templates, which are inhibited due to the ligation of E. coli DNA ligase. With this novel strategy, a set of logic gates is successfully constructed at the molecular level,including “YES”, “PASS 0”, “OR”, “INHIBIT”, which take the advantages of no label, easy operation, fast speed, high efficiency and low cost. Furthermore, S1 nuclease, as the biomarker of numerous carcinogens,is selectively detected in the range of 0.05–50 U/m L with the detection limit of 0.005 U/m L(1×10^(−6)U)based on this platform.

关键词： Logic gates Dumbbell DNA templates Poly-thymine loops Copper nanoclusters Nucleic acid enzymes

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Dephosphorylation of cardiomyocyte Cx43 is associated with myocardial ischemia and reperfusion injury

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Journal of Nanjing Medical University 2009年第3期23卷 163-167页

作者： zhijuan cao Xuan Xu Linli Que Qi Chen Yuehua Li Department of Pathophysiology Nanjing Medical University Nanjing 210029 China

Objective：Myocardial ischemia/reperfusion（I/R） injury is the leading cause of death in the world. However, the details of the mechanism of its pathophysiology are still unknown. The present study was designed to investigate the role of connexin 43（Cx43） in acute models of myocardial I/R injury. Methods： Male C57BL/6 mice were subjected to myocardial ischemia（45 min） followed by reperfusion（4 hrs） in vivo. The whole operation was monitored using a two-lead ECG. Hearts were harvested and the level of protein was assessed by western blot analysis. Haematoxylin and Eosin（HE） staining was used to detect the extent of neutrophil infiltration. The expression level of IL-6 was detected by ELISA. Results： A murine myocardial I/R injury model was constructed successfully. Phosphorylated Cx43 decreased 83. 45% while non-phosphorylated Cx43 increased 1.62- fold in the myocardium after I/R injury. Neutrophil infiltration and the expression of the inflammatory cytokine IL-6 increased in the myocardium following I/R. Conclusion： During myocardial I/R injury, cardiomyocyte Cx43 is dephosphorylated, and this may be associated with an inflammatory response.

关键词： myocardium I/R injury connexin 43 inflammatory response

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Magnetic covalent organic framework nanospheres-based miRNA biosensor for sensitive glioma detection

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Bioactive Materials 2022年第8期7卷 145-151页

作者： Dong Liang Xiaoyi Zhang Yi Wang Taotao Huo Min Qian Yibo Xie Wenshuai Li Yunqiu Yu Wei Shi Qianqian Liu Junle Zhu Chun Luo zhijuan cao Rongqin Huang School of Pharmacy Key Laboratory of Smart Drug DeliveryMinistry of EducationFudan UniversityShanghai201203China Center for Advanced Low-dimension Materials State Key Laboratory for Modification of Chemical Fibers and Polymer MaterialsCollege of ChemistryChemical Engineering and BiotechnologyDonghua UniversityShanghai201600China Department of Neurosurgery Affiliated Hospital of Nantong UniversityNantong226001China Department of Neurosurgery Tongji HospitalTongji University School of MedicineShanghai200065China

Sensitive detection and accurate diagnosis/prognosis of glioma remain urgent ***,dispersed magnetic covalent organic framework nanospheres(MCOF)with uniformed Fe3O4 nano-assembly as cores and high-crystalline COF as shells were prepared by monomer-mediated in-situ interface growth *** on the unique interaction between MCOF and hairpin DNA,a fluorescent signal amplified miRNA biosensor was *** could realize the sensitive detection of miRNA-182 in different matrixes,where the detection limit,linearity range and determination coefficient(R^(2))in real blood samples reached 20 fM,0.1 pM-10 pM and 0.991,***,it possessed good stability and precision as observed from the low intra-day/inter-day RSD and high extraction *** a result,it could quantify miRNA-182 in serum of glioma patients,the concentration of which was significantly higher than that of healthy people and obviously decreased after ***,a proof-of-concept capillary chip system using this biosensor was proposed to realize the visualized detection of miRNA-182 in *** findings suggest a robust way for sensitive detection and accurate diagnosis/prognosis of glioma.

关键词： Blood samples Fluorescence signal amplification biosensor Glioma diagnosis Magnetic COF nanospheres miRNA detection

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Nonhematopoietic erythropoietin derivative protects cardiomyocytes from hypoxia/reoxygenation-induced apoptosis

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Journal of Nanjing Medical University 2008年第2期22卷 71-74页

作者： Xuan Xu Xiaohong Shan zhijuan cao Meiling Wu Qi Chen Yuehua Li Department of Pathophysiology Nanjing Medical University Nanjing 210029 China

Objective：Carbamylated EPO（CEPO） is a derivative of erythropoietin（EPO） by subjecting it to carbamylation. It does not stimulate erythropoiesis, but effectively protects tissue from injury. The present study was to investigate the effect of CEPO treatment using in vitro models of hypoxia/reoxygenation（H/R）. Methods：Cardiomyocytes were exposed to hypoxia（95% N2 and 5% CO2） for 1 hour followed by 4 hours of reoxygenation（95% O2 and 5% CO2）. CEPO was administered after hypoxia, just before reoxygenation. The apoptotic cardiomyocytes were determined by flow cytometry. The level of protein was assessed by western blot analysis. Results： CEPO treatment significantly decreased the apoptotic cardiomyocytes by 54.20% compared with H/R group. Western blot analysis showed that CEPO administration increased the level of Bcl-2（an antiapoptotic protein） by 62.22% compared with H/R group. Conclusion： Acute administration of CEPO protected cardiomyocytes from H/R-induced apoptosis. CEPO protected cardiomyocytes with a concomitant upregulation of Bcl-2 after H/R injury.

关键词： carbamylated erythropoietin hypoxia/reoxygenation cardiomyocytes apoptosis

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基于二氧杂环丁烷骨架的化学发光探针发展和应用研究

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化学进展 2023年第2期35卷 189-205页

作者：傅安辰毛彦佳王宏博曹志娟复旦大学药学院上海201203 中国医药工业研究总院科技成果转化中心上海201203

光学分析法具有无损、实时和空间分辨能力的特点,是研究疾病发生、发展和诊疗的一种重要技术手段,包括荧光、生物发光和化学发光(CL)。近年来,以金刚烷稳定的二氧杂环丁烷为骨架的化学发光探针(AD⁃CL)获得关注。该探针不但具有无需激发... 详细信息

光学分析法具有无损、实时和空间分辨能力的特点,是研究疾病发生、发展和诊疗的一种重要技术手段,包括荧光、生物发光和化学发光(CL)。近年来,以金刚烷稳定的二氧杂环丁烷为骨架的化学发光探针(AD⁃CL)获得关注。该探针不但具有无需激发光源,可避免光漂白、光毒性,灵敏度较高的CL法传统优势;且具有检测体系简单,无需氧化剂的参与,可在生理条件下发光等特点。最近,经过进一步的结构优化与改造,AD⁃CL骨架探针在生理条件下的发光性能大幅提升,在物质检测、光学成像等领域中的应用越来越广泛。本文综述了AD⁃CL探针的发光原理以及最新的研究进展,主要分为两部分:AD⁃CL探针的优化与改造策略以及AD⁃CL探针在不同物质检测的应用。

关键词：二氧杂环丁烷化学发光探针分子设计光学成像

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批判性思维:初中英语阅读教学的“磨刀石”

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英语教师 2019年第5期19卷 130-132页

作者：曹志娟常州外国语学校江苏常州213000

随着新课程改革的实施,培养学生的批判性思维已经成为初中英语阅读教学的重要任务。在教学中,教师要形成批判观念,采用有效的策略,发展学生的批判性阅读能力,提升学生的思维品质,培养学生的质疑精神,促使学生全面发展。结合教学实践,从... 详细信息

随着新课程改革的实施,培养学生的批判性思维已经成为初中英语阅读教学的重要任务。在教学中,教师要形成批判观念,采用有效的策略,发展学生的批判性阅读能力,提升学生的思维品质,培养学生的质疑精神,促使学生全面发展。结合教学实践,从创设教学环境、设计批判问题、培养质疑意识、科学批判四个角度分析如何实施初中英语批判性阅读教学,从而培养学生的批判性思维。

关键词：批判性思维初中英语阅读教学

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Aperiodically semi-intermittent-based fixed-time stabilization and synchronization of delayed discontinuous inertial neural networks

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Science China(Information Sciences) 2025年第1期68卷 238-254页

作者： Guodong ZHANG Jinde cao School of Mathematics and Statistics South-Central Minzu University School of Mathematics Southeast University

This article concentrates on the fixed-time stabilization(FS) and fixed-time synchronization(FTS) of discontinuous inertial neural networks(DINNs) with distributed delays. Using mathematical induction to a new differential equality, a novel fixed-time stability lemma is constructed. Then, by designing an aperiodically semi-intermittent switching control and combining it with the theory of nonsmooth analysis, some novel criteria on the FS and FTS of DINNs are obtained. Unlike the methods used in most existing studies, the FS and FTS results are structured using the newly proposed fixed-time stability lemma and the nonreduced-order approach, resulting in broader practical applications and strengthening the scientific quality of the derived results. Lastly, numerical simulations and applications proffer the effectiveness of the established FS and FTS criteria.

关键词： inertial neural networks fixed-time synchronization semi-intermittent switching control nonreduced-order approach fixed-time stabilization

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Experimental Observing Damage Evolution in Cement Pastes Exposed to External Sulfate Attack by in situ X-ray Computed Tomography

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 164-170页

作者： WU Min cao Kailei XIAO Weirong YU Zetai cao Jierong DING Qingjun LI Jinhui China Railway Construction Corporation Harbour and Channel Engineering Bureau Group Co. LTDZhuhai 519000China Con-struction Headquarters of Zhoushan Liuheng Bridge Phase II Project Zhoushan 316000China China Railway Construction Bridge En-gineering Bureau Group Co. LTDTianjin 300000China State Key Laboratory of Silicate Material for Architecture(Wuhan University of Technology) Wuhan 430070China College of Materials Science and Engineering Wuhan Textile UniversityWuhan 430200China

The paper presents experimental investigation results of crack pattern change in cement pastes caused by external sulfate attack(ESA).To visualize the formation and development of cracks in cement pastes under ESA,an X-ray computed tomography(X-ray CT)was used,i e,the tomography system of Zeiss Xradia 510 *** results indicate that X-CT can monitor the development process and distribution characteristics of the internal cracks of cement pastes under ESA with attack *** addition,the C3A content in the cement significantly affects the damage mode of cement paste specimens during sulfate *** damage of ordinary Portland cement(OPC)pastes subjected to sulfate attack with high C3A content are severe,while the damage of sulfate resistant Portland cement(SRPC)pastes is much smaller than that of OPC ***,a quadratic function describes the correlation between the crack volume fraction and development depth for two cement pastes immermed in sulfate solution.

关键词： concrete external sulfate attack damage evolution situ X-ray computed tomography

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上海市小学生家长健康素养现况

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中国学校卫生 2018年第4期39卷 498-500,505页

作者：曹志娟王书梅曲爽笑同济大学附属第一妇婴保健院上海201204 复旦大学公共卫生学院上海市疾病预防控制中心

目的了解上海市小学生家长的健康素养水平,为促进小学生健康素养提升提供良好的家庭环境。方法2015年9—10月期间,采用方便抽样法选取上海市中心城区4所小学、郊区2所小学共3 283名小学生,对其家长进行《中国公民健康素养调查问卷》调... 详细信息

目的了解上海市小学生家长的健康素养水平,为促进小学生健康素养提升提供良好的家庭环境。方法2015年9—10月期间,采用方便抽样法选取上海市中心城区4所小学、郊区2所小学共3 283名小学生,对其家长进行《中国公民健康素养调查问卷》调查。结果在全部参与调查的家长中,具备健康素养的家长占21.6%。随着父母受教育程度的提高,具备健康素养的家长占比也逐渐提高(χ~2值分别为110.500,113.886,P值均<0.01)。户籍为"上海市"和"外国国籍"的学生家长具备健康素养的比例相当(均为24.56%),均高于非上海市的中国户籍学生家长(16.69%)(χ~2=27.771,P<0.01)。父、母职业为农业劳动者的健康素养具备率均低于其他职业(P值均<0.01)。此外,家长对安全标志的辨识,正确率仅为0.27%;"蚊子、苍蝇、老鼠和蟑螂传播疾病的种类"正确率仅为7.58%;"您是否能做到不与他人共用毛巾"正确率仅为10.94%。结论开展小学生健康素养促进工作要加强对低文化水平、非上海籍在上海就读中国学生家长的关注。

关键词：健康教育家长作风学生

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镍纳米片的制备、表征及摩擦学应用

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江苏大学学报（自然科学版） 2010年第4期31卷 417-421页

作者：张锡凤程晓农严冲曹智娟郝伟刘晓光江苏大学化学化工学院江苏镇江212013 江苏大学材料科学与工程学院江苏镇江212013

采用液相化学还原结合界面生长法,以醋酸镍为母体,水合肼为还原剂,吐温-40为修饰剂,NaOH为pH调节剂,正丁醇为生长剂,制备了厚度15~27 nm、片长380~500 nm的镍纳米片.通过X射线衍射、扫描电子显微镜和激光粒度仪对镍纳米片进行了表征.将... 详细信息

采用液相化学还原结合界面生长法,以醋酸镍为母体,水合肼为还原剂,吐温-40为修饰剂,NaOH为pH调节剂,正丁醇为生长剂,制备了厚度15~27 nm、片长380~500 nm的镍纳米片.通过X射线衍射、扫描电子显微镜和激光粒度仪对镍纳米片进行了表征.将镍纳米片、球形镍纳米颗粒作为基础油液体石蜡（LP）的添加剂,在UMT-Ⅱ摩擦磨损实验机上分别考察添加前、添加后LP的摩擦磨损性能.结果表明,镍纳米片的晶格参数膨胀率为0.971%,各晶面2θ相应增加;与基础油LP相比,添加纳米镍后,较大程度降低了摩擦副的摩擦因数,显著改善了LP的摩擦性能;就改善效果而言,镍纳米片优于球形镍纳米颗粒.

关键词：镍纳米片化学还原法界面生长法晶格参数摩擦学性能

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