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The modification effects of roselle anthocyanin film on shrimp texture via water distribution controlling and protein conformation maintenance

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Food Bioscience 2022年第5期49卷 872-883页

作者： Jiayin huang zhiheng hu Lingping hu Gaoshang Li Chunhong Yuan Jianchu Chen Yaqin hu Institute of Food Engineering College of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhouZhejiang310058China College of Food Science and Engineering Hainan Tropical Ocean UniversitySanyaHainan572022China Yazhou Bay Innovation Institute SanyaHainan572022China d Marine Food Engineering Technology Research Center of Hainan ProvinceSanyaHainan572022China Collaborative Innovation Center of Marine Food Deep Processing SanyaHainan572022China Hainan Key Laboratory of Herpetological Research College of Fisheries and Life ScienceHainan Tropical Ocean UniversitySanyaHainan572022China Department of Food Production and Environmental Management Faculty of AgricultureIwate UniversityUeda4-3-5Morikoa020-8551Japan

The food’s perishable properties and people’s eco-friendly requirements have driven the biodegradable active packaging *** this study,modification behaviors of polyviniyl alcohol/hydroxypropyl methylcellulose/roselle anthocyanin-based(PHR)film on textural profiles of Penaeus vannamei were *** samples were packaged by PHR film with different roselle anthocyanin contents and stored at 4±1℃.The results indicated that PHR groups exhibited better texture with higher values of hardness(435.56±75.52 g),springiness(0.53±0.04 mm),chewiness(198.83±40.98 N)and resilience(0.45±0.04).The PHR film exerted positive influences on water migration by reducing drip loss and promoting conversion of free water into immobilized ***,the PHR film retarded protein denaturation via increasing myofibrillar protein and sulfhydryl group contents,decreasing TCA(trichloroacetic acid)-soluble peptide contents and maintaining protein secondary structure(higherα-helix ratio and lowerβ-sheet ratio).In conclusion,the PHR film improved shrimp texture by rearrangement of water distribution and stabilization of protein ***,the PHR film was a novel potential active packaging for shrimp texture enhancement.

关键词： Active packaging Textural properties Water migration Protein conformation

Inhibition of citral nanoemulsion to growth,spoilage ability and Al-2/luxS quorum sensing system of Shewanella putrefaciens CN-32:a study on bacteriostasis from in vitro culture and gene expression analysis

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Food Quality and Safety 2022年第3期6卷 435-445页

作者： zhiheng hu Yaoxian Chin Jiayin huang Jiaying Zhou Gaoshang Li Yaqin hu Chunhong Yuan Jianchu Chen College of Biosystems Engineering and Food Science National-Local Joint Engineering Laboratory of Intelligent Food Technology and EquipmentZhejiang Key Laboratory for Agro-Food ProcessingIntegrated Research Base of Southern Fruit and Vegetable Preservation TechnologyFuli Institute of Food ScienceZhejiang UniversityHangzhouChina College of Food Science and Engineering Hainan Tropical Ocean UniversityYazhou Bay Innovation InstituteMarine Food Engineering Technology Research Center of Hainan ProvinceCollaborative Innovation Center of Marine Food Deep ProcessingSanyaChina Department of Food Production and Environmental Management Faculty of AgricultureIwate UniversityUeda4-3-5MoriokaJapan

Objectives:The bacteriostatic effects of a citral nanoemulsion against Shewanella putrefaciens CN-32(SHP CN-32)were investigated using in vitro culture and gene expression analysis,forbuilding a potential application in spoilage microorganism control and aquatic products quality *** and Methods:SHP CN-32 was treated by prepared citral nanoemulsion when the minimal inhibitory concentration(MIC)was *** growth curve,membrane integrity,scanning electron microscope(SEM)observation,biofilm formation and quorum sensing(QS)signaling molecule Al-2 content were evaluated in different MIC treatment groups(0 to 1.00 MIC).The gene expression status of SHP CN-32 in O and 0.50 MIC groups were compared using transcriptome sequencing and quantitative polymerase chain reaction(PCR).Results:The in vitro culture revealed that the citral nanoemulsion could inhibit the growth of SHP CN-32 with MIC of approximately 200μg/*** of membrane *** and biofilm formation suggested significant biological structure damage in bacteria after ***,the Qs signaling molecule Al-2 content showed a decline with increasing treatment *** sequencing and quantitative PCR revealed that the majority genes related diversified functional metabolic pathways of SHP CN-32 were downregulated at varying ***:A significant bacteriostasis of citral nanoemulsion against SHP CN-32 was verified via the results of growth inhibition,structural destruction,signal molecular decrease and gene expression downregulation of *** synergies significantly affect the characteristic expression of SHP CN-32,revealing the application potential as bacteriostat,QS inhibitor and preservative in aquatic products.

关键词： Shewanella putrefaciens citral quorum sensing system gene expression analysis

Interfacial characteristics and mechanical properties of additive manufacturing martensite stainless steel on the Cu-Cr alloy substrate by directed energy deposition

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Journal of Materials Science & Technology 2021年第31期90卷 121-132页

作者： Wenqi Zhang Hailong Liao zhiheng hu Shasha Zhang Baijin Chen huanqing Yang Yun Wang Haihong Zhu Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan 430074China State Key Laboratory of Material Processing and Die&Mould Technology School of Material Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China XI’AN Space Engine Company Limited Xi’an710100China

Copper/steel is a typical bimetal functional material,combining the excellent electrical and thermal conductivity of copper alloy and the high strength and hardness of stainless *** has been recent interest in manufacturing copper/steel bimetal by directed energy deposition(DED)due to its layer-bylayer ***,cracks tend to form on the copper/steel interface because of the great difference in thermal expansion coefficient and crystal structure between copper and *** this work,interfacial characteristics and mechanical properties of the copper/steel bimetal were studied from one layer to *** laser power has a great influence on the Cu element distribution of the molten pool,affecting the crack formation dramatically on the solidification *** tend to form along columnar grain boundaries because of the Cu-rich liquid films and spherical particles in the ***-free and good metallurgical bonding copper/steel interface is formed at a scanning velocity of 800 mm/min and the laser power of 3000 *** ultimate tensile strength(UTS)and the break elongation(EL)of the vertically combined crack-free copper/steel bimetal are 238.2±4.4 MPa and 20.6±0.7%,*** fracture occurs on the copper side instead of the copper/steel interface,indicating that the bonding strength is higher than that of the Cu-Cr *** UTS of the horizontally combined crack-free copper/steel bimetal is 746.7±22.6 MPa,which is 200%higher than that of the Cu-Cr alloy *** microhardness is 398.6±5.4 HV at the steel side and is 235.3±64.1 HV at the interface,which is400%higher than that of the Cu-Cr alloy *** paper advances the understanding of the interfacial characteristics of heterogeneous materials and provides guidance and reference for the fabrication of multi-material components by DED.

关键词： Directed energy deposition Additive manufacturing Bimetal Interfacial characteristics Crack

3D printing properties and printability definition of Pennahiaargentata surimi and rice starch

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Food Bioscience 2022年第4期48卷 283-299页

作者： Gaoshang Li Junqi Zhan zhiheng hu Jiayin huang Xunyu Luo Jianchu Chen Chunhong Yuan Koichi Takaki Yaqin hu Institute of Food Engineering College of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhou310058ZhejiangChina College of Food Science and Engineering Hainan Tropical Ocean UniversityMarine Food Engineering Technology Research Center of Hainan ProvinceCollaborative Innovation Center of Marine Food Deep ProcessingSanya572022China Department of Food Production and Environmental Management Faculty of AgricultureIwate UniversityUeda 4-3-5Morioka020-8551Japan Faculty of Science and Engineering Iwate UniversityUeda 4-3-5Morioka020-8551Japan School of food science and biotechnology Zhejiang Gongshang UniversityHangzhou310000ZhejiangChina

Correlation of related factors(texture,gel strength,rheological properties)were analyzed for better characterize 3D material ***,printability calculation formula also was established to represent material *** argentata surimi shown best plasticity with 78%moisture content and 20%rice starch ***,starch and carrageenan increased gel strength,yield stress of surimi and starch respectively,which brought better mechanical strength and supporting *** also affected viscosity of surimi(increasing)and starch(decreasing),which brought better fluidity and adhesion respectively,resulting in good printability,especially starch(8%)-surimi,carrageenan(0.5%)-*** on their interaction relationships,3D printability equation of printing inkswas established their relevance and corresponding unified *** judge the printability of same materials under different conditions were consistent with real print *** could provide methods for judging material printability by specific values and promote the application and spread of 3D materials.

关键词： 3D print Texture properties Gel strength Rheological properties Printability equation

Development of Micro Selective Laser Melting:The State of the Art and Future Perspectives

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Engineering 2019年第4期5卷 702-720页

作者： Balasubramanian Nagarajan zhiheng hu Xu Song Wei Zhai Jun Wei Singapore Institute of Manufacturing Technology(SIMTech) Agency for ScienceTechnology and Research(A^*STAR)Singapore 637662Singapore

Additive manufacturing(AM)is gaining traction in the manufacturing industry for the fabrication of components with complex geometries using a variety of *** laser melting(SLM)is a common AM technique that is based on powder-bed fusion(PBF)to process metals;however,it is currently focused only on the fabrication of macroscale and mesoscale *** paper reviews the state of the art of the SLM of metallic materials at the microscale *** comparison with the direct writing techniques that are commonly used for micro AM,micro SLM is attractive due to a number of factors,including a faster cycle time,process simplicity,and material versatility.A comprehensive evaluation of various research works and commercial systems for the fabrication of microscale parts using SLM and selective laser sintering(SLS)is *** addition to identifying existing issues with SLM at the microscale,which include powder recoating,laser optics,and powder particle size,this paper details potential future directions.A detailed review of existing recoating methods in powder-bed techniques is conducted,along with a description of emerging efforts to implement dry powder dispensing methods in the AM domain.A number of secondary finishing techniques for AM components are reviewed,with a focus on implementation for microscale features and integration with micro SLM systems.

关键词： Additive manufacturing Selective laser melting Microfabrication Hybrid processing Powder-bed recoating

Early cancer detection by serum biomolecular fingerprinting spectroscopy with machine learning

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eLight 2023年第1期3卷 94-104页

作者： Shilian Dong Dong He Qian Zhang Chaoning huang zhiheng hu Chenyang Zhang Lei Nie Kun Wang Wei Luo Jing Yu Bin Tian Wei Wu Xu Chen Fubing Wang Jing hu Xiangheng Xiao Department of Physics National Demonstration Center for Experimental Physics EducationWuhan UniversityWuhan 430072China Department of Laboratory Medicine Zhongnan Hospital of Wuhan UniversityWuhan 430071China School of Computer Science Wuhan UniversityWuhan 430072China Department of Hepatobiliary Pancreatic Surgery Hubei Cancer HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430079China Department of Clinical Laboratory Tianjin Medical University General HospitalTianjin 300052China Department of Blood Transfusion Wuhan Hospital of Traditional Chinese and Western MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Laboratory of Printable Functional Materials and Printed Electronics Research Center for Graphic CommunicationPrinting and PackagingWuhan UniversityWuhan 430072China Sichuan Provincial Key Laboratory for human Disease Gene Study and the Center for Medical Genetics Department of Laboratory MedicineSichuan Academy of Medical Sciences&Sichuan Provincial People’s HospitalUniversity of Electronic Science and TechnologyChengdu 611731China School of Medicine University of Electronic Science and Technology of ChinaChengdu 611731China Wuhan Research Center for Infectious Diseases and Cancer Chinese Academy of Medical SciencesWuhan 430071China

Label-free surface-enhanced Raman scattering(SERS)technique with ultra-sensitivity becomes more and more desirable in biomedical analysis,which is yet hindered by inefficient follow-up data *** we report an integrative method based on SERS and Artificial Intelligence for Cancer Screening(SERS-AICS)for liquid biopsy such as serum via silver nanowires,combining molecular vibrational signals processing with large-scale data mining *** to 382 healthy controls and 1582 patients from two independent cohorts,SERS-AICS not only distinguishes pan-cancer patients from health controls with 95.81% overall accuracy and 95.87% sensitivity at 95.40% specificity,but also screens out those samples at early cancer *** supereminent efficiency potentiates SERS-AICS a promising tool for detecting cancer with broader types at earlier stage,accompanying with the establishment of a data platform for further deep analysis.

关键词： analysis specificity silver

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受压区局部增强的钢筋混凝土梁抗剪性能

深圳大学学报（理工版） 2021年第5期38卷 479-488页

作者：周英武胡智恒胡彪深圳大学土木与交通工程学院广东省滨海土木工程耐久性重点实验室广东深圳518060

钢筋混凝土梁受压区的应力状态和材料性能对抗剪承载力和变形能力的影响显著.用性能更好的纤维增强水泥基复合材料(engineered cementitious composite,ECC)替换受压区混凝土,以增强钢筋混凝土梁的抗剪性能,是一个崭新的尝试.设计了4条... 详细信息

钢筋混凝土梁受压区的应力状态和材料性能对抗剪承载力和变形能力的影响显著.用性能更好的纤维增强水泥基复合材料(engineered cementitious composite,ECC)替换受压区混凝土,以增强钢筋混凝土梁的抗剪性能,是一个崭新的尝试.设计了4条全尺寸的钢筋混凝土梁,研究受压区ECC对有/无箍筋梁抗剪性能的影响;为得到准确的箍筋和混凝土的抗剪贡献,在箍筋内部安装应变片还原真实的箍筋抗剪贡献;为了解耦弯曲挠度和剪切挠度,采用非接触式数字图像系统测量混凝土表面的应变场.结果表明:受压区ECC改变了梁的破坏过程;对于无箍筋梁,受压区ECC的存在使得构件抗剪承载力和挠度分别提高了92.0%和97.3%,对于有箍筋梁,抗剪承载力和挠度提高幅度分别为5.2%和26.8%;在主斜裂缝发展过程中,普通混凝土梁的混凝土抗剪贡献缓慢下降,而受压区用ECC替换的梁则是先缓慢减小然后逐渐增大直至剪切破坏;用两种方法解耦了梁的弯曲挠度和剪切挠度,二者结果吻合较好;现有典型规范不能准确的预测混凝土与箍筋的抗剪贡献.

关键词：结构工程钢筋混凝土梁抗剪强度受压区纤维增强水泥基复合材料剪切挠度

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拦截导弹的制导律辨识与弹道预报

系统工程与电子技术 2018年第3期40卷 609-614页

作者：胡志恒周荻邹昕光哈尔滨工业大学航天学院黑龙江哈尔滨150001 哈尔滨工业大学电气工程及自动化学院黑龙江哈尔滨150001

讨论了拦截导弹的制导律辨识和弹道预报问题。在三维状态下,以相对距离、相对速度、导弹加速度、两通道制导系数作为系统的状态变量,建立了相遇过程中的观测模型。用非线性系统局部弱可观理论分析了系统可观的条件,然后在此基础上设计... 详细信息

讨论了拦截导弹的制导律辨识和弹道预报问题。在三维状态下,以相对距离、相对速度、导弹加速度、两通道制导系数作为系统的状态变量,建立了相遇过程中的观测模型。用非线性系统局部弱可观理论分析了系统可观的条件,然后在此基础上设计了扩展卡尔曼滤波器。以滤波结果为初值,用四阶龙格库塔方法基于比例导引律的模型对导弹未来的位置进行了预报。仿真结果表明,在视线角速率较大的情况下滤波器可以取得较好的效果,弹道预报可以满足一定的精度和速度要求。

关键词：比例导引律非线性系统可观性扩展卡尔曼滤波器弹道预报

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AlSi10Mg的激光选区熔化成形研究

金属学报 2017年第8期53卷 918-926页

作者：张文奇朱海红胡志恒曾晓雁华中科技大学武汉光电国家实验室武汉430074

利用激光选区熔化(selective laser melting,SLM)成形技术对Al Si10Mg铸造铝合金的成形进行了工艺研究,获得了致密的成形,并对其沉积态和热处理态试样进行了静态拉伸性能测试和显微组织分析。结果表明:对于Al Si10Mg,其SLM沉积态的常温... 详细信息

利用激光选区熔化(selective laser melting,SLM)成形技术对Al Si10Mg铸造铝合金的成形进行了工艺研究,获得了致密的成形,并对其沉积态和热处理态试样进行了静态拉伸性能测试和显微组织分析。结果表明:对于Al Si10Mg,其SLM沉积态的常温拉伸强度远高于铸件标准,延伸率与铸态相当;退火工艺对SLM试样的组织及力学性能有着重要的影响,随着退火温度的提高,试样微观组织发生改变,在300℃、2 h退火工艺下,原本均匀分布的颗粒状Si聚集长大为针状,使得试样的强度下降,延伸率升高。抗拉强度由沉积态的507~518 MPa下降到378~406 MPa,延伸率由沉积态的3.0%~3.5%增加到6.5%~9.5%。

关键词： AlSi10Mg 激光选区熔化力学性能热处理