检索结果-南通市图书馆

Biomimetic Phosphohydrolase Nanozyme Based on Defect-Engineered Metal-Organic Framework

在线全文

学校读者我要写书评

暂无评论

CCS Chemistry 2024年第7期6卷 1821-1835页

作者： Xiaoxue kou Yuhong Lin Yong Shen Linjing Tong Rui Gao Suya Liu Siming Huang Fang Zhu Guosheng Chen Gangfeng Ouyang MOE Key Laboratory of Bioinorganic and Synthetic Chemistry School of ChemistrySun Yat-sen UniversityGuangzhou 510006 Shanghai NanoPort Thermo Fisher ScientificPudong DistrictShanghai 200120 Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target and Clinical Pharmacology The NMPA and State Key Laboratory of Respiratory DiseaseSchool of Pharmaceutical Sciences and The Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou 511436

Phosphodiester bonds are the backbone linkages of all the nucleic acids which store and transfer biological *** hydrolysis of phosphodiester bonds is of great importance to the replication,recombination,and damage repair of DNA as well as the maturation and processing of ***,the spontaneous scission of individual phosphodiester linkages is kinetically challenging under physiological conditions,with an estimated half-life of 30 million ***,we discover a defect-engineered Zr-metal-organic framework(Zr-MOF)nanozyme containing cluster-missing reo defects,named UiO-66 REO,that possesses intrinsic phosphodiesterase-like activity that allows for the cleavage of the phosphodiester bond in physiological condition(pH=7.4 and 37℃),outperforming the activityrecording *** atomic-level structure of the UiO-66 REO nanozyme has been directly identified by the synchrotron radiation absorption spectrum and integrated differential phase contrast-scanning transmission electron *** find that both the defective boundary regions and unusual Zr6-clusters formed in the reo phase of the UiO-66 REO nanozyme contribute to the improvement in efficiency of phosphodiesterase-like hydrolysis,which realizes the cleavage of DNA in mild *** work offers a new insight into biomimetic enzymology using crystal and defect engineering and opens up new possibilities for the development of low-cost,structurally stable and sustainable phosphodiesterase nanozymes for different biological applications.

关键词： nanozyme metal-organic framework biomimetic catalysis hydrolase-like activity defective engineering

An integrated microfluidic platform for nucleic acid testing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Microsystems & Nanoengineering 2024年第3期10卷 181-194页

作者： Antao Sun Petra Vopařilová Xiaocheng Liu Bingqian kou TomášŘezníček TomášLednický Sheng Ni JiříKudr Ondřej Zítka Zdenka Fohlerová Petr Pajer Haoqing Zhang Pavel Neužil Ministry of Education Key Laboratory of Micro and Nano Systems for Aerospace School of Mechanical EngineeringNorthwestern Polytechnical University127 West Youyi RoadXi’anShaanxi 710072P.R.China Department of Chemistry and Biochemistry Mendel University in BrnoZemědělská161300 BrnoCzech Republic ITD Tech s.r.o Osvoboditelů100573581 BohumínCzech Republic Central European Institute of Technology Brno University of TechnologyPurkyňova 123Brno 61200Czech Republic Department of Electronic and Computer Engineering The Hong Kong University of Science and TechnologyClear Water BayKowloonHong Kong SARChina Department of Microelectronics Faculty of Electrical Engineering and CommunicationBrno University of TechnologyTechnická3058/10Brno 61600Czech Republic Military Health Institute U Vojenskénemocnice 120016200 Praha 6Czech Republic The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and TechnologyXi’an Jiaotong UniversityXi’anShaanxi 710049P.R.China Bioinspired Engineering and Biomechanics Center(BEBC) Xi’an Jiaotong UniversityXi’an 710049P.R.China

This study presents a rapid and versatile low-cost sample-to-answer system for SARS-CoV-2 *** system integrates the extraction and purification of nucleic acids,followed by amplification via either reverse transcriptionquantitative polymerase chain reaction(RT–qPCR)or reverse transcription loop-mediated isothermal amplification(RT–LAMP).By meeting diverse diagnostic and reagent needs,the platform yields testing results that closely align with those of commercial RT-LAMP and RT‒qPCR *** advantages of our system include its speed and *** assay is completed within 28 min,including sample loading(5 min),ribonucleic acid(RNA)extraction(3 min),and RT-LAMP(20 min).The cost of each assay is≈$9.5,and this pricing is competitive against that of Food and Drug Administration(FDA)-approved commercial *** some RNA loss during on-chip extraction is observed,the platform maintains a potential limit of detection lower than 297 *** makes the system particularly useful in environments where centralized laboratories are either unavailable or inconveniently *** key feature is the platform’s versatility,allowing users to choose between RT‒qPCR or RT‒LAMP tests based on specific requirements.

关键词： testing maintain versatile

Neglected Time Intervals Before “1-3-7” Approach About Imported Malaria Cases-China, 2014–2021

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

China CDC weekly 2024年第17期6卷 363-367页

作者： Zhiquan He Yuanjing kou Dan Wang Ying Liu Xiaofeng Lyu Hui Yan Wen Lin Xuan Zhang Duoquan Wang Shizhu Li Hongwei Zhang Henan Provincial Center for Disease Control and Prevention Zhengzhou CityHenan ProvinceChina Anhui Provincial Center for Disease Control and Prevention Hefei CityAnhui ProvinceChina Guangxi Zhuang autonomous Region Center for Disease Control and Prevention Nanning CityGuangxi Zhuang Autonomous RegionChina Hubei Provincial Center for Disease Control and Prevention Wuhan CityHubei ProvinceChina Zhejiang Provincial Center for Disease Control and Prevention Hangzhou CityZhejiang ProvinceChina National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research)National Health Commission Key Laboratory of Parasite and Vector BiologyShanghaiChina School of Global Health Chinese Center for Tropical Diseases ResearchShanghai Jiao Tong University School of MedicineShanghaiChina

What is already known about this topic?China’s“1-3-7”approach outlines specific targets to guide and monitor the processes of case reporting,investigation,and ***,few studies have examined the time intervals preceding the initial step,and the timeline from the arrival of imported malaria cases in China to their diagnosis has been largely *** is added by this report?The study demonstrated that the median duration from arrival in China to the onset of symptoms for *** was 78 days,with 71.59%of imported cases manifesting symptoms after more than one *** ***,the median interval was 42 days,with 55.91%exceeding one ***,the median time from symptom onset to malaria treatment in China between 2014 and 2021 was 2 days,with an interquartile range(IQR)of 1-4 *** are the implications for public health practice?This study represents the initial effort to delineate the chronology of imported malaria cases,from their arrival in China to their subsequent *** results underscore the importance of providing malaria health education to populations arriving from ***,enhancing physician training is crucial for improving the diagnosis of malaria.

关键词： diagnosis Malaria exceeding

Mitogenomic phylogeny,biogeography,and cryptic divergence of the genus Silurus(Siluriformes:Siluridae)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Zoological Research 2024年第4期45卷 711-723页

作者： Weitao Chen Nicolas Hubert Yuefei Li Shuli Zhu Jun Wang Denggao Xiang Shang Gao Chunni kou Jilong Wang Tai Wang Zhiqiang Liang Junjie Wu Xinhui Li Jie Li Pearl River Fisheries Research Institute Chinese Academy of Fishery SciencesGuangzhouGuangdong 510380China China Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province GuangzhouGuangdong 510380China Key Laboratory of Prevention and Control for Aquatic Invasive Alien Species Ministry of Agriculture and Rural AffairsGuangzhouGuangdong 510380China Scientific Observing and Experimental Station of National Fisheries Resources and Environment GuangzhouGuangdong 510380China Institut de Recherche pour le Développement MontpellierUMR 226 ISEM(UM-CNRS-IRD)France State Key Laboratory of Eco-hydraulic in Northwest Arid Region of China Xi’an University of TechnologyXi’anShaanxi 710048China Heilongjiang River Fisheries Research Institute Chinese Academy of Fishery SciencesHarbinHeilongjiang 150076China Gansu Fisheries Research Institute LanzhouGansu 730030China Hunan Fisheries Science Institute ChangshaHunan 410153China Yunnan Institute of Fishery Sciences Research KunmingYunnan 650111China

The genus Silurus,an important group of catfish,exhibits heterogeneous distribution in Eurasian freshwater *** group includes economically important and endangered species,thereby attracting considerable scientific *** this interest,the lack of a comprehensive phylogenetic framework impedes our understanding of the mechanisms underlying the extensive diversity found within this ***,we analyzed 89 newly sequenced and 20 previously published mitochondrial genomes(mitogenomes)from 13 morphological species to reconstruct the phylogenetic relationships,biogeographic history,and species diversity of *** phylogenetic reconstructions identified eight clades,supported by both maximum-likelihood and Bayesian ***-based species delimitation analyses yielded multiple molecular operational taxonomic units(MOTUs)in several taxa,including the Silurus asotus complex(four MOTUs)and Silurus microdorsalis(two MOTUs),suggesting that species diversity is underestimated in the genus.A reconstructed time-calibrated tree of Silurus species provided an age estimate of the most recent common ancestor of approximately 37.61 million years ago(Ma),with divergences among clades within the genus occurring between 11.56 Ma and 29.44 Ma,and divergences among MOTUs within species occurring between 3.71 Ma and 11.56 *** reconstructions suggested that the ancestral area for the genus likely encompassed China and the Korean Peninsula,with multiple inferred dispersal events to Europe and Central and Western Asia between 21.78 Ma and 26.67 Ma and to Japan between 2.51 Ma and 18.42 *** factors such as the Eocene-Oligocene extinction event,onset and intensification of the monsoon system,and glacial cycles associated with sea-level fluctuations have likely played significant roles in shaping the evolutionary history of the genus Silurus.

关键词： Maximum likelihood Bayesian inferences Ancestral area estimation Cryptic diversity Dispersal pathways Mitogenomes Sea levels

在线全文

学校读者我要写书评

暂无评论

马克思剩余劳动概念的伦理意蕴

理论视野 2024年第6期292卷 5-10页

作者：寇东亮陕西师范大学哲学学院

剩余劳动概念在马克思剩余价值理论的创建和唯物史观的完善中具有至关重要的意义,它关涉剩余价值源泉、剥削秘密、脑体分工、物质财富、自由时间、人的全面发展等主题,具有丰富的伦理意蕴。这主要表现在三个方面:一是通过“劳动力商品... 详细信息

剩余劳动概念在马克思剩余价值理论的创建和唯物史观的完善中具有至关重要的意义,它关涉剩余价值源泉、剥削秘密、脑体分工、物质财富、自由时间、人的全面发展等主题,具有丰富的伦理意蕴。这主要表现在三个方面:一是通过“劳动力商品”批判和“剩余劳动时间”分析,凸显剩余劳动概念的生命伦理意蕴;二是通过“脑体劳动分工”批判和“剩余劳动产品”分析,凸显剩余劳动概念的劳动伦理意蕴;三是通过“为生产而生产”批判和“剩余劳动积累”分析,凸显剩余劳动概念的财富伦理意蕴。

关键词：马克思剩余劳动生命财富伦理

在线全文

学校读者我要写书评

暂无评论

自媒体时代大学生审美伦理危机及消解机制

江苏高教 2024年第5期 81-86页

作者：寇思华东师范大学美术学院上海200062

自媒体时代大学生呈现出审美标准媚俗化、价值判断去崇高化、审美认知去客观化等审美伦理危机。社会发展过程中的“个体化”趋势是危机泛起原因。首先,个体化导致自媒体时代大学生缺少整体审美标准,无法实现道德社会化。其次,个体化导... 详细信息

自媒体时代大学生呈现出审美标准媚俗化、价值判断去崇高化、审美认知去客观化等审美伦理危机。社会发展过程中的“个体化”趋势是危机泛起原因。首先,个体化导致自媒体时代大学生缺少整体审美标准,无法实现道德社会化。其次,个体化导致自媒体时代的大学生缺少共同的道德榜样,消解了理性崇高。最后,自媒体时代大学生个体化道德关系不稳定,无法实现审美伦理共识。对于上述危机,需要合理、妥善地引导大学生重建个体化与公共性的认知,寻找与当代中国审美趣味和时代精神相契合的公共性审美标准,也需要制定以允许为原则的公共性规约,在道德共识的基础上树立道德榜样,还需要构建公共性道德关系,达成自媒体时代大学生的审美伦理共识。

关键词：自媒体时代大学生审美伦理个体化公共性

Multimodal imaging in the diagnosis of bone giant cell tumors:A retrospective study

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2024年第16期12卷 2722-2728页

作者： Ming-Qing kou Bing-Qiang Xu Hui-Tong Liu Department of Medical Imaging Shaanxi Provincial People's HospitalXi'an 710068Shaanxi ProvinceChina Department of Orthopedics Shaanxi Provincial People's HospitalXi'an 710068Shaanxi ProvinceChina

BACKGROUND Giant cell tumor of bone is a locally aggressive and rarely metastasizing tumor,and also a potential malignant tumor that may develop into a primary malignant giant cell *** To evaluate the role of multimodal imaging in the diagnosis of giant cell tumors of *** The data of 32 patients with giant cell tumor of bone confirmed by core-needle biopsy or surgical pathology at our hospital between March 2018 and March 2023 were retrospectively *** the patients with giant cell tumors of the bone were examined by X-ray,computed tomography(CT)and magnetic resonance imaging(MRI),and 7 of them were examined by positron emission tomography(PET)-*** X-ray imaging can provide overall information on giant cell tumor *** and MRI can reveal the characteristics of the internal structure of the tumor as well as the adjacent relationships of the tumor,and these methods have unique advantages for diagnosing tumors and determining the scope of ***-CT can detect small lesions and is highly valuable for identifying benign and malignant tumors to aid in the early diagnosis of *** Multimodal imaging plays an important role in the diagnosis of giant cell tumor of bone and can provide a reference for the treatment of giant cell tumors.

关键词： Giant cell tumor of bone Multimodal imaging Computed tomography Magnetic resonance imaging Positron emission tomography-computed tomography

间谍、越境、修辞:南北朝跨境交流中的“言”和“志”

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

文艺研究 2024年第1期 44-60页

作者：寇陆哥伦比亚大学东亚语言与文化系

南北朝时期,政权之间信息和情报不断被交换、传递、解读。而对于诸如间谍、流寓敌国的皇族和羁旅之臣等跨境者来说,不仅他们带来的信息会被甄别和评断,他们自身也在被新朝“细读”,以从其“言行”推断“心志”。对跨境者“内”“外”不... 详细信息

南北朝时期,政权之间信息和情报不断被交换、传递、解读。而对于诸如间谍、流寓敌国的皇族和羁旅之臣等跨境者来说,不仅他们带来的信息会被甄别和评断,他们自身也在被新朝“细读”,以从其“言行”推断“心志”。对跨境者“内”“外”不一的怀疑以及跨境者在诗文中的自我塑造,让“志”和“言”之间充满了张力和不确定性。从南北政权之间信息战和文化战的角度来看,跨境者的言辞展现出“言”与“志”、“外”与“内”、“迹”与“心”对应的复杂性,以及跨境者如何利用修辞术进行自我言说,从而应对政治、军事、文化竞争中不断变化的局势。在跨境与文化竞争的语境下,“言”不再是“志”的自然外露,而是由多朝参与、虚实混淆、表意复杂的阐释事件,而跨境者的修辞作用也是在读者对其“志”与“言”对应关系的探求中生成、变化的。

关键词：自我塑造修辞术自我言说修辞作用羁旅之臣跨境不确定性南北朝时期

Loss of Tet hydroxymethylase activity causes mouse embryonic stem cell differentiation bias and developmental defects

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2024年第10期67卷 2132-2148页

作者： Mengting Wang Liping Wang Yanxin Huang Zhibin Qiao Shanru Yi Weina Zhang Jing Wang Guang Yang Xinyu Cui Xiaochen kou Yanhong Zhao Hong Wang Cizhong Jiang Shaorong Gao Jiayu Chen Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital Shanghai Key Laboratory of Signaling and Disease ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China Shanghai Tenth People’s Hospital School of MedicineTongji UniversityShanghai 200072China Frontier Science Center for Stem Cell Research Tongji UniversityShanghai 200092China Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of the Ministry of Education Orthopaedic Department of Tongji HospitalTongji UniversityShanghai 200065China Shanghai Institute of Stem Cell Research and Clinical Translation Shanghai 200120China

The TET family is well known for active DNA demethylation and plays important roles in regulating transcription,the epigenome and ***,previous studies using knockdown(KD)or knockout(KO)models to investigate the function of TET have faced challenges in distinguishing its enzymatic and nonenzymatic roles,as well as compensatory effects among TET family members,which has made the understanding of the enzymatic role of TET not accurate *** solve this problem,we successfully generated mice catalytically inactive for specific Tet members(Tetm/m).We observed that,compared with the reported KO mice,mutant mice exhibited distinct developmental defects,including growth retardation,sex imbalance,infertility,and perinatal ***,Tetm/mmouse embryonic stem cells(mESCs)were successfully established but entered an impaired developmental program,demonstrating extended pluripotency and defects in ectodermal differentiation caused by abnormal DNA ***,Tet3,traditionally considered less critical for m ESCs due to its lower expression level,had a significant impact on the global hydroxymethylation,gene expression,and differentiation potential of ***,there were common regulatory regions between Tet1 and Tet3 in pluripotency *** summary,our study provides a more accurate reference for the functional mechanism of Tet hydroxymethylase activity in mouse development and ESC pluripotency regulation.

关键词： Tet hydroxymethylase activity mESCs DNA demethylation 5hmC