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Gut fungal community and its probiotic effect on Bactrocera dorsalis

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Insect Science 2022年第4期29卷 1145-1158页

作者： Qiongyu Guo zhichao yao Zhaohui Cai Shuai Bai Hongyu Zhang Key Laboratory of Horticultural Plant Biology(MOE) Hubei Hongshan LaboratoryChina-Australia Joint Research Centre for Horticultural and Urban PestsInstitute of Urban and Horticultural EntomologyCollege of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina

The oriental fruit fly,Bactrocera dorsalis(Diptera:Tephritidae)is a destructive horticultural pest which causes considerable economic losses every year.A collection of microorganisms live within the *** gut,and they are involved in its development,physiology,and ***,knowledge regarding the composition and function of the gut mycobiota in *** are still ***,we comprehensively characterized the gut mycobiota in *** across different developmental ***-throughput sequencing results showed a significant difference in fungal species abundance and diversity among different developmental stages of *** polymerase chain reaction and culture-dependent methods showed that yeast species was the dominant group in the larval *** isolated 13 strains of yeast from the larval gut,and found that GF(germ-free)larvae mono-associated with strain Hanseniaspora uvarum developed faster than those mono-associated with other tested fungal *** the larval diet with *** fully rescued *** development,shortened the larval developmental time,and increased adult wing lengths,as well as the body sizes and weights of both pupae and ***,our study highlights the close interactions between gut fungi,especially ***,and *** findings can be applied to the sterile insect technique program to promote host development during mass insect rearing.

关键词： Baclroceru edlorsalis fungi Hanseniaspor unvarum intestinal microbes Tephritidae fruit flies

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串联型颈动脉狭窄的血流动力学模拟分析

介入放射学杂志 2024年第2期33卷 150-155页

作者：曹俊杰姚志超霍桂军刘占鳌汤尧黄剑周大勇沈利明南京医科大学姑苏学院苏州市立医院南京医科大学附属苏州医院血管外科江苏南京215000

目的使用计算流体力学(computational fluid dynamic,CFD)对串联型颈动脉狭窄(tandem carotid artery stenosis,TCAS)进行模拟,比较不同手术方式术后的血流动力学改变情况。方法选取1例颈内动脉(internal carotid artery,ICA)和颈总动脉(common carotid artery,CCA)串联型狭窄患者,使用CFD技术对建立的4个颈动脉分叉三维模型(1个真实患者模型,3个假定手术模型)进行血流动力学分析,探索TCAS的发生发展机制并指导选择合适的手术方案。结果在串联型狭窄中,狭窄优先在CCA形成,随后导致ICA狭窄;TCAS较单个颈动脉狭窄的局部血流动力学情况更复杂,风险更高;串联型狭窄中,单个狭窄部位的治疗会影响第二个狭窄部位的血流,并可能导致再狭窄或斑块破裂。结论在规划TCAS手术方案时,对选定TCAS患者进行CFD模拟可帮助临床医生评估术后血流改变情况,指导选择合适的手术方案。

关键词：颈动脉狭窄串联病变血流动力学计算流体力学

Anodic process of stibnite in slurry electrolysis:The direct collision oxidation

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Chinese Journal of Chemical Engineering 2022年第1期35卷 466-472页

作者： Yonglu Zhang Dingfan Qiu Chengyan Wang Yongqiang Chen zhichao yao Xiaowu Jie Wei Gao Shufeng Ruan State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China BGRIMM Technology Group Beijing 100160China

Mineral oxidation leaching in the anode area is the key step in slurry *** adopting the slow linear potential scanning method during slurry electrolysis,this study investigated the steady-state polarization curve of a pure stibnite mineral on a graphite *** addition,the influence of the mineral particle size,liquid–solid ratio,stirring speed,and temperature on the collision oxidation of the mineral with the anode was *** on the different oxidation reactions,the potential range can be divided into three intervals:the low-potential interval with a potential lower than 0.75 V,an intermediatepotential interval with a potential within 0.75–1.2 V,and a high-potential interval with a potential higher than 1.2 *** collision oxidation of the mineral with the anode occurred in all three *** oxidation of Sb(III)also appeared in the intermediate-and high-potential intervals,and chlorine evolution occurred in the high-potential ***,the low-potential interval was determined to be a suitable potential interval for the slurry electrolysis *** the low-potential interval,the particle size,liquid–solid ratio,and stirring speed had little effect on the oxidation rate of the *** the temperature increased,the stibnite oxidation rate and exchange current density ***,the direct collision oxidation rate of stibnite was relatively low and the current densities under all the investigated conditions were lower than 0.4 mA·cm^(-*** indicates that it is difficult to realize industrial production while relying solely on this process.

关键词： Slurry electrolysis Stibnite Potential range Potential interval Steady-state polarization curve

钠离子电池正极材料磷酸焦磷酸铁钠的研究进展

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南京工业大学学报（自然科学版） 2024年第3期46卷 235-250页

作者：张鼎周启坤刘镇铭胡新颖叶子贤药志超李卫武汉工程大学化工与制药学院湖北武汉430205 浙江瓦司特钠科技有限公司浙江嘉兴314000 内蒙古师范大学物理与电子信息学院内蒙古呼和浩特010000

钠离子电池(SIB)是继锂离子电池(LIB)后,最具有应用前景的电池技术之一,而磷酸焦磷酸铁钠是一种具备较高可逆比容量、长循环寿命的复合型钠离子电池聚阴离子型正极材料。系统地总结了磷酸焦磷酸铁钠的制备方法与电化学性能,揭示了磷酸... 详细信息

钠离子电池(SIB)是继锂离子电池(LIB)后,最具有应用前景的电池技术之一,而磷酸焦磷酸铁钠是一种具备较高可逆比容量、长循环寿命的复合型钠离子电池聚阴离子型正极材料。系统地总结了磷酸焦磷酸铁钠的制备方法与电化学性能,揭示了磷酸焦磷酸铁钠材料具备的基本结构特性与储钠电极反应机制。同时,分析了已有的磷酸焦磷酸铁钠正极材料的改性方法和电化学性能提升机制,展望了该领域的发展趋势,指出深入的理论研究和工艺改进是重要的研究方向。

关键词：钠离子电池聚阴离子正极材料磷酸焦磷酸铁钠制备方法电化学性能

The Q181X Point Mutation in Nf1 Induces Cerebral Vessel Stenosis

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Neuroscience Bulletin 2023年第5期39卷 813-816页

作者： Chensi Liang Lirong Huo Yan Zhu zhichao yao Xiaolong Wu Jiantao Liang Central Laboratory Department of NeurologyFuxing HospitalCapital Medical UniversityBeijing 100038China Neurosurgery of Xuanwu Hospital Capital Medical UniversityBeijing 100053China

Dear Editor,Ischemic stroke is characterized by high morbidity and disability,seriously endangering human *** cause and genetic background of cerebrovascular stenosis,which directly lead to stroke,are not fully understood yet[1].Therefore,it is diffcult to accurately predict the development of stroke and carry out the corresponding prevention and ***1(neurofibromatosis type 1)is one of the most common autosomal dominant genetic diseases,caused by mutations in the Nfl gene with an incidence rate of~1/3000 and there are no ethnic or sex differences in the incidence[2].More than 8%of NF1 patients will have hypertension,arterial stenosis,aortic aneurysm,or moyamoya disease[3].

关键词： prevention stenosis diseases

球囊辅助顺行栓塞术联合经颈静脉肝内门体静脉分流术在门静脉高压性食管胃底静脉曲张破裂出血治疗中的初步探索

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血管与腔内血管外科杂志 2024年第4期10卷 456-460页

作者：曹俊杰姚志超曾宇琪周大勇沈利明汤尧南京医科大学姑苏学院/南京医科大学附属苏州医院/苏州市立医院(本部)血管外科江苏苏州215002

目的探索球囊辅助顺行栓塞术(BA-PTVE)联合经颈静脉肝内门体静脉分流术(TIPS)治疗门静脉高压性食管胃底静脉曲张破裂出血(EGVB)的安全性及有效性。方法收集2020年6月至2021年5月南京医科大学姑苏学院/南京医科大学附属苏州医院/苏州市... 详细信息

目的探索球囊辅助顺行栓塞术(BA-PTVE)联合经颈静脉肝内门体静脉分流术(TIPS)治疗门静脉高压性食管胃底静脉曲张破裂出血(EGVB)的安全性及有效性。方法收集2020年6月至2021年5月南京医科大学姑苏学院/南京医科大学附属苏州医院/苏州市立医院(本部)收治的16例门静脉高压性EGVB患者的临床资料,所有患者均采用BA-PTVE联合TIPS进行治疗。观察所有患者的技术成功率、治疗成功率、手术时间、住院时间及术后并发症发生情况,以及术后1年再出血率、生存率和肝性脑病的发生率。结果技术成功和治疗成功的患者均为16例,平均手术时间为(146.4±37.9)min,平均住院时间为(15.2±3.5)d。3例患者术后出现腹部疼痛,1例患者术后出现肝性脑病,1例患者术后出现弥散性血管内凝血,其余患者未发生手术相关并发症。术后1年,15例患者无再出血事件和死亡事件发生,2例患者发生肝性脑病。结论BA-PTVE联合TIPS治疗门静脉高压性EGVB初步被证实是安全、有效的。

关键词：球囊辅助顺行栓塞术食管胃底静脉曲张破裂出血经皮经肝食管胃底静脉曲张栓塞术经颈静脉肝内门体静脉分流术

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多模态影像学对颈动脉斑块易损性的诊断价值

血管与腔内血管外科杂志 2024年第3期10卷 270-274页

作者：曾宇琪郑进霍桂军曹俊杰姚志超沈利明周大勇南京医科大学姑苏学院/南京医科大学附属苏州医院/苏州市立医院(本部)血管外科江苏苏州215002

目的探讨计算机断层扫描血管成像(CTA)、磁共振成像(MRI)、超声及CTA+超声对颈动脉斑块易损性的诊断效能。方法收集2020年10月至2023年5月南京医科大学姑苏学院/南京医科大学附属苏州医院/苏州市立医院(本部)行颈动脉血管重建术的26... 详细信息

目的探讨计算机断层扫描血管成像(CTA)、磁共振成像(MRI)、超声及CTA+超声对颈动脉斑块易损性的诊断效能。方法收集2020年10月至2023年5月南京医科大学姑苏学院/南京医科大学附属苏州医院/苏州市立医院(本部)行颈动脉血管重建术的26例患者的临床资料,将其手术所得颈动脉斑块行苏木精-伊红(HE)染色、Masson三色染色及CD34抗体染色,分析斑块成分,判断斑块稳定性。以病理结果为金标准,比较不同影像学方法的诊断效能。结果以病理结果为金标准,CTA+超声评估易损斑块的灵敏度最高,MRI评估易损斑块的特异度、准确度、阳性预测值、阴性预测值均较高。MRI对斑块的脂质核心、薄纤维帽、溃疡、钙化、斑块内出血检出率与病理结果比较,差异均无统计学意义(P﹥0.05),MRI检测斑块薄纤维帽的灵敏度最高,检测斑块脂质核心的特异度最高,检测斑块薄纤维帽、脂质核心的准确度均较高。结论 MRI仍是评估颈动脉粥样硬化斑块的金标准,但CTA+超声评估颈动脉粥样硬化斑块的灵敏度、准确度与MRI相当,且其更加快速、便捷和经济,更利于在基层医院推广应用。

关键词：颈动脉狭窄易损斑块磁共振成像计算机断层扫描血管成像 B超

Regulatory mechanisms of microbial homeostasis in insect gut

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Insect Science 2021年第2期28卷 286-301页

作者： Shuai Bai zhichao yao Muhammad Fahim Raza Zhaohui Cai Hongyu Zhang State Key Laboratory of Agricultural Microbiology Key Laboratory of Horticultural Plant Biology(MOE)China-Australia Joint Research Centre for Horticultural and Urban PestsInstitute of Urban and Horticultural EntomologyCollege of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina

Insects live in incredibly complex *** intestinal epithelium of insects is in constant contact with microorganisms,some of which are beneficial and some harmful to the *** gut health and function are maintained through multidimensional mechanisms that can proficiently remove foreign pathogenic microorganisms while effectively maintaining local symbiotic microbial *** basic immune mechanisms of the insect gut,such as the dual oxidase-reactive oxygen species(Duox-ROS)system and the immune deficiency(Imd)-signaling pathway,are involved in the maintenance of microbial *** paper reviews the role of physical defenses,the Duox-ROS and Imd signaling pathways,the Janus kinase/signal transducers and activators of transcription signaling pathway,and intestinal symbiotic flora in the homeostatic maintenance of the insect gut microbiome.

关键词： Duox-ROS immune system gut microbiota immune deficiency signaling pathway insect intestinal immunity peritrophic matrix

Crocus genome reveals the evolutionary origin of crocin biosynthesis

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1878-1891页

作者： zhichao Xu Shanshan Chen Yalin Wang Ya Tian Xiaotong Wang Tianyi Xin Zishan Li Xin Hua Shengnan Tan Wei Sun Xiangdong Pu Hui yao Ranran Gao Jingyuan Song Key Lab of Chinese Medicine Resources Conservation State Administration of Traditional Chinese Medicine of the People's Republic of ChinaInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China State Key Laboratory of Basis and New Drug Development of Natural and Nuclear Drugs Engineering Research Center of Chinese Medicine ResourceMinistry of EducationBeijing 100193China College of Life Science Northeast Forestry UniversityHarbin 150040China Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine China Academy of Chinese Medical SciencesInstitute of Chinese Materia MedicaBeijing 100700China Analysis and Testing Center of Northeast Forestry University Harbin 150040China School of Pharmacy Anhui Medical UniversityInflammation and Immune Mediated Diseases Laboratory of Anhui ProvinceHefei 230032China

Crocus sativus (saffron) is a globally autumn-flowering plant, and its stigmas are the most expensive spice and valuable herb medicine. Crocus specialized metabolites, crocins, are biosynthesized in distant species, Gardenia (eudicot) and Crocus (monocot), and the evolution of crocin biosynthesis remains poorly understood. With the chromosome-level Crocus genome assembly, we revealed that two rounds of lineage-specific whole genome triplication occurred, contributing important roles in the production of carotenoids and apocarotenoids. According to the kingdom-wide identification, phylogenetic analysis, and functional assays of carotenoid cleavage dioxygenases (CCDs), we deduced that the duplication, site positive selection, and neofunctionalization of Crocus-specific CCD2 from CCD1 members are responsible for the crocin biosynthesis. In addition, site mutation of CsCCD2 revealed the key amino acids, including I143, L146, R161, E181, T259, and S292 related to the catalytic activity of zeaxanthin cleavage. Our study provides important insights into the origin and evolution of plant specialized metabolites, which are derived by duplication events of biosynthetic genes.

关键词： Crocus sativus WGT Crocin biosynthesis Carotenoids Apocarotenoids CCDs