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Arabidopsis AGC protein kinases IREH1 and IRE3 control root skewing

Journal of Genetics and Genomics 2019年第5期46卷 259-267页

作者： Xiaozhen Yue zhiai guo Teng Shi Lizhen Song Youfa Cheng Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China

AGC protein kinases play important roles in plant growth and *** AGC kinases in Arabidopsis have been functionally ***,the"AGC Other"subfamily,including IRE,IREH1,IRE3 and IRE4,has not been well ***,we reported that ireh1 mutants displayed a root skewing phenotype,which can be enhanced by ire3 ***1 and IRE3 were expressed in roots,consistent with their function in controlling root *** fluorescence intensities of the microtubule marker KNpro:EGFP-MBD were decreased in ireh1,ire3 and ireh1 ire3 mutants compared to wild *** microtubule arrangements in ireh1 and ireh1 ire3 mutants were also ***1 physically interacted with IRE3 in vitro and in ***,our findings demonstrate that IREH1 and IRE3 protein kinases play important roles in controlling root skewing,and maintaining microtubule network in Arabidopsis.

关键词： AGC kinases IREH1 IRE3 Root skewing Microtubule Arabidopsis

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NCP1/At MOB1A plays key roles in auxin-mediated Arabidopsis development

NCP1/At MOB1A plays key roles in auxin-mediated Arabidopsis ...

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2016年全国植物生物学大会

作者： Xiaona Cui zhiai guo Lizhen Song Yanli Wang Youfa Cheng Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of Sciences University of Chinese Academy of Sciences

MOB1 protein is a core component of the Hippo signaling pathway in animals,where it is involved in controlling tissue growth and tumor suppression. Plant MOB1 proteins display high sequence homology to animal MOB1 proteins,but little is known regarding their role in plant growth and development. Herein we report the critical roles of Arabidopsis MOB1(At MOB1A) in auxin-mediated development in Arabidopsis. We found that loss-of-function mutations in At MOB1 A completely eliminated the formation of cotyledons when combined with mutations in PINOID(PID),which encodes a Ser/Thr protein kinase that participates in auxin signaling and transport. We show ed that atmob1 a was fully rescued by its Drosophila counterpart,suggesting functional conservation. The atmob1 a pid double mutants phenocopied several well-characterized mutant combinations that are defective in auxin biosynthesis or transport. Moreover,we demonstrated that atmob1 a greatly enhanced several other known auxin mutants,suggesting that At MOB1 A plays a key role in auxinmediated plant development. The atmob1 a single mutant displayed defects in early embryogenesis and had shorter root and smaller flowers than wild type plants. At MOB1 A is uniformly expressed in embryos and suspensor cells during embryogenesis,consistent with its role in embryo development. At MOB1 A protein is localized to nucleus,cytoplasm,and associated to plasma membrane,suggesting that it plays roles in these subcellular localizations. Furthermore,we showed that disruption of At MOB1 A led to a reduced sensitivity to exogenous auxin. Our results demonstrated that At MOB1 A plays an important role in Arabidopsis development by promoting auxin signaling.

关键词： NCP1/At MOB1A Hippo pathway auxin embryogenesis

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Circadian clock gene TaPRR1,associated with yield-related traits,underwent selection during wheat breeding

Circadian clock gene TaPRR1,associated with yield-related tr...

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第十届全国小麦基因组学及分子育种大会

作者： Han Sun Wenping Zhang Yongzhen Wu Lifeng Gao Guangyao Zhao Fa Cui Chunhua Zhao Hailong An zhiai guo Jizeng Jia College of Agriculture Ludong University National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop ScienceChinese Academy of Agricultural Sciences State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural University Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of Sciences

Wheat is an important food crop and the timing of flowering is the key stage for the transformation from vegetative to reproductive growth *** clock is the central part of photoperiod regulation and Pseudo-Response Regulators(PRR) are key components of circadian ***,little is known about the effects of PRR family members on yield-related traits of crop *** this study,we reported polymorphisms and haplotypes of TaPRR1 and demonstrated that natural variations of TaPRRl associated with significant differences on yield-related traits including plant height,thousand grain weight and heading *** localization and transcriptional activity analysis showed that TaPRRl is a nuclear localization protein with transcriptional activity controlled by CCT *** addition,geographical distribution analysis indicated that TaPRR1 haplotypes showed distinctive characteristics in different agro-ecological production ***,assessment of the distribution frequency of TaPRR1 haplotypes suggested that TaPRR1 underwent selection during wheat breeding *** study will provide basis for indepth understanding of the function of TaPRR1 on yield-related traits of wheat,as well as establish a theoretical guidance for wheat molecular marker-assisted breeding.

关键词： Wheat TaPRR1 haplotype association analysis yield selection

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基于“辨状论质”综合评价指数的拳参药材规格等级标准研究

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中华中医药学刊 2024年第9期42卷 240-245,I0049页

作者：郭超伟徐文丽徐荣陈乐詹志来刘大会杜鸿志湖北中医药大学药学院中药资源与中药化学湖北省重点实验室湖北武汉430065 中国中医科学院中药资源中心道地药材国家重点实验室培育基地北京100700

针对市场拳参只有统货规格,基于辩证论质法,建立拳参商品规格等级标准。首先,对24批拳参外观性状和数量指标进行测定。同时,检测浸出物、总酚酸、没食子酸三个内在成分,并对拳参抗氧化药理活性进行评价。随后,为了探究可能作为拳参药材... 详细信息

针对市场拳参只有统货规格,基于辩证论质法,建立拳参商品规格等级标准。首先,对24批拳参外观性状和数量指标进行测定。同时,检测浸出物、总酚酸、没食子酸三个内在成分,并对拳参抗氧化药理活性进行评价。随后,为了探究可能作为拳参药材规格等级的参数,分析单个外观指标与内在质量指标相关性;并采用信息熵赋权法对内在质量指标整体进行综合评分,进一步地分析内在质量指标综合评分与外观性状的相关性。最后,运用描述统计分析、主成分分析、方差分析确定性状指标的重要度。内在指标综合评分、总酚酸含量与长度值1、最粗直径、单个质量、L*、b*呈极显著正相关;浸出物与长度值1、L*呈极显著正相关。抗氧化能力与长度值1、最粗直径、单个质量、L*、b*呈极显著正相关。筛选得到最粗直径、单个质量、百克个数三个重要性状指标,制定拳参商品规格等级分为统货、选货,统货断面颜色偏暗,呈棕褐色,直径在0.83.0 cm;选货断面颜色鲜亮,偏黄,分为2等,一等品拳参最粗直径在1.8 cm以上,单个质量6.9 g以上,百克个数16个以内,综合评分≥64.63;二等品拳参最粗直径1.5 cm以上,单个质量3.8 g以上,百克个数33个以内,综合评分≥35.74。研究证实了本草记载的科学性,即个大、断面浅棕色为佳,制定了拳参商品规格等级标准,利于其市场环境和质量控制,确保临床用药的稳定性和有效性。

关键词：拳参外观性状内在质量信息熵赋权法辨状论质

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液化石油气铜片腐蚀不合格的研究分析

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石油化工应用 2023年第6期42卷 100-103页

作者：葛玉翠罗致爱郭智慧袁晓云赵湘庆贾红博陕西煤业化工技术研究院有限责任公司陕西西安710100 中国石油长庆石化分公司陕西咸阳712000

针对中国石油长庆石化分公司液化石油气铜片腐蚀不合格现象,对引起液化石油气铜片腐蚀不合格的原因进行了分析。分析结果表明硫化氢及硫化物不是导致铜片腐蚀不合格的主要原因,由于原料变化,导致原料中含有含氧化合物,并最终形成有机酸... 详细信息

针对中国石油长庆石化分公司液化石油气铜片腐蚀不合格现象,对引起液化石油气铜片腐蚀不合格的原因进行了分析。分析结果表明硫化氢及硫化物不是导致铜片腐蚀不合格的主要原因,由于原料变化,导致原料中含有含氧化合物,并最终形成有机酸,此类酸通过MDEA和脱硫剂无法去除,导致液化石油气铜片腐蚀不合格。

关键词：液化石油气腐蚀硫化氢有机酸

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