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Progress in diagnosis and treatment of acute injury to the anterior talofibular ligament

World Journal of Clinical Cases 2023年第15期11卷 3395-3407页

作者： Run-Peng Chen Qing-Hua Wang Ming-Yue li Xiao-Fang Su Dong-Yang Wang Xing-Hui liu zhi-li li School of Nursing Binzhou Medical UniversityYantai 264003Shandong ProvinceChina Faculty of Nursing Mahidol UniversityNakhon Pathom 73170Thailand Department of Office Shandong Vheng Data Technology Co.LtdYantai 264003Shandong ProvinceChina

Injury to the anterior talofibular ligament(ATFL)is a common acute injury of the lateral foot ligament.Untimely and improper treatment significantly affects the quality of life and rehabilitation progress of patients.The purpose of this paper is to review the anatomy and the current methods of diagnosis and treatment of acute injury to the ATFL.The clinical manifestations of acute injury to the ATFL include pain,swelling,and dysfunction.At present,non-surgical treatment is the first choice for acute injury of the ATFL.The standard treatment strategy involves the“peace and love”principle.After initial treatment in the acute phase,personalized rehabilitation training programs can be followed.These may involve proprioception training,muscle training,and functional exercise to restore limb coordination and muscle strength.Static stretching and other techniques to loosen joints,acupuncture,moxibustion massage,and other traditional medical treatments can relieve pain,restore range of motion,and prevent joint stiffness.If the non-surgical treatment is not ideal or fails,surgical treatment is feasible.Currently,arthroscopic anatomical repair or anatomical reconstruction surgery is commonly used in clinical practice.Although open Broström surgery provides good results,the modified arthroscopic Broström surgery has many advantages,such as less trauma,rapid pain relief,rapid postoperative recovery,and fewer complications,and is more popular with patients.In general,when treating acute injury to the ATFL,treatment management and methods should be timely and reasonably arranged according to the specific injury scenario and attention should be paid to the timely combination of multiple therapies to achieve the best treatment results.

关键词： Anterior talofibular ligament Acute injury Diagnosis Aetiology Treatment

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Direct observation of the autophosphorylation of insulin receptor kinase by mass spectrometry

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Chinese Chemical Letters 2009年第2期20卷 204-206页

作者： zhi li li Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and School of Basic Medicine Peking Union Medical College Beijing 100005 China

The catalytic and signaling activities of insulin receptor kinase （IRK） are regulated by the autophosphorylation of three tyrosine residues in a cytoplasmic protein-tyrosine kinase domain at Tyro 1158, Tyro 1162 and Tyro 1163. In this study, time-course of the auphosphorylation of the core kinase （residues 978-1283） from IRK was directly investigated by online electrospray ionization mass spectrometry. It is found that two tyrosine residues were phosphorylated in reaction time range of 30 min. This study implies that mass spectrometric technique must be a powerful tool to directly monitor the biological macromolecular modification and will also provide the information of the order and the mechanism of autophosphorylation at the tyrosine sites coupled with tandem mass spectrometric technique.

关键词： Phosphorylation Insulin receptor kinase Mass spectrometry Time course

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Slime coating of kaolinite on chalcopyrite in saline water flotation

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International Journal of Minerals,Metallurgy and Materials 2018年第5期25卷 481-488页

作者： zhi-li li Feng Rao Shao-xian Song Yan-mei li Wen-biao liu CONACYT Instituto de Investigación en Metalurgiay Materiales Universidad Michoacana de San Nicolás de Hidalgo School of Resources and Environmental Engineering Wuhan University of Technology Departamento de Ingeniería en Minas Metalurgia y GeologíaUniversidad de Guanajuato Faculty of Land and Resource Engineering Kunming University of Science and Technology

In saline water flotation, the salinity can cause a distinguishable slime coating of clay minerals on chalcopyrite particles through its effect on their electrical double layers in aqueous solutions. In this work, kaolinite was used as a representative clay mineral for studying slime coating during chalcopyrite flotation. The flotation of chalcopyrite in the presence and absence of kaolinite in tap water, seawater, and gypsum-saturated water and the stability of chalcopyrite and kaolinite particles in slurries are presented. Zeta-potential distributions and scanning electron microscopy images were used to characterize and explain the different slime coating degrees and the different flotation performances. Kaolinite particles induced slime coating on chalcopyrite surfaces and reduced chalcopyrite floatability to the greatest extent when the p H value was in the alkaline range. At 0.24 wt% of kaolinite, the chalcopyrite floatability was depressed by more than 10% at alkaline p H levels in tap water. Salinity in seawater and gypsum-saturated water compressed the electrical double layers and resulted in extensive slime coating.

关键词： chalcopyrite clay minerals saline water flotation slime coating stability

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Poplar catkin:A natural biomaterial for highly specific and efficient enrichment of sialoglycopeptides

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Chinese Chemical Letters 2017年第5期28卷 1018-1026页

作者： Xiao-Dong Wang Yu-Jie liu Fen-Jie li zhi-li li Department of Biophysics and Structural Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences&School of Basic MedicinePeking Union Medical CollegeBeijing 100005China

Sialic acids as terminal entities of larger glycans linked to proteins and lipids are involved in multiple different pathological and physiological processes.Structural characterisation of sialoglycoconjugates is required to understand their biological function.However,a comprehensive sialylation analysis of sialoglycoconjugates has remained challenges.In this study,we employ a natural biomaterial,poplar catkin derived from white poplar tree（Populus tomentosa Carr.）,to develop a novel capturing microtip for selective and efficient enrichment of sialoglycopeptides,without losses of sialic acid residues and water molecules from sialoglycopeptides.Scanning electron microscopy and Fourier-transform infrared spectroscopy analysis,along with Maule and Wiesner staining assays,indicated that the main components on the outer layer of the poplar catkin are syringyl and guaiacyl lignins which play a key role in enriching sialoglycopeptides from complex peptide mixture.

关键词： Sialogycopeptide Poplar catkin Enrichment Natural biomaterial Mass spectrometry

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Investigation of the Structures of the Product Ions Produced via self-CI of Acetone

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Chinese Chemical Letters 1998年第7期9卷 675-678页

作者： zhi li li Shu Ying liU(Changchun Institute of Applied Chendstry, Chinese Academy of Sciences,Changchun 130022) Changchun Inst. of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China

The ion-molecule reactions in acetone were investigated which were induced under the chemical ionization. The structural information of the reaction products were obtained by using collision-induced dissociation (CID)... 详细信息

关键词： ion-molecule reaction acetone ion structure collision-induced dissociation

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低分子质量肝素锂的制备及其体外抗凝血性能评价

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现代化工 2024年第5期44卷 170-174,181页

作者：蔡鑫鑫袁建梅李曰珍谭新强李志利高宾汪应灵新乡医学院基础医学院河南新乡453003 河南省驼人医疗科技有限公司河南新乡453000

以肝素钠粗品为原料,采用超声盐解-阳离子交换转锂-二氧化氯氧化-醇沉-干燥的新型离子交换工艺制备低分子质量肝素锂。采用Box-Behnlen实验对制备工艺条件进行优化。通过FT-IR、UV-Vis、1HNMR、XPS、比旋光度等对产品进行组成、结构和... 详细信息

以肝素钠粗品为原料,采用超声盐解-阳离子交换转锂-二氧化氯氧化-醇沉-干燥的新型离子交换工艺制备低分子质量肝素锂。采用Box-Behnlen实验对制备工艺条件进行优化。通过FT-IR、UV-Vis、1HNMR、XPS、比旋光度等对产品进行组成、结构和性能表征,考察了其活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)、凝血酶时间(TT)体外抗凝3项指标。结果表明,盐解辅以超声、转锂时锂型树脂交换、ClO_(2)氧化脱色为该工艺改良的3个关键环节。最佳工艺条件下制得肝素锂效价为173.3 IU/mg,锂质量分数为3.6%,比旋光度为+51.422°。自制肝素锂与肝素钠标准品具有高度相似的结构特征,保留了肝素中—OSO_(3)^(-)、—COO^(-)、OH^(-)等活性基团。自制肝素锂展现出较强的体外抗凝血活性,主要通过内源凝性途径和共同途径作用于凝血过程,可作为医用采血器的预置抗凝剂。

关键词：肝素锂新型离子交换法超声盐解二氧化氯氧化抗凝血性能

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FOURIER TRANSFORM INFRARED SPECTROSCOPIC STUDY OF EFFECT OF LANTHANIDE IONS ON PHOSPHOliPID MULTIBILAYERS

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Chinese Chemical Letters 1992年第10期3卷 829-832页

作者： Wei WANG Lai Ming li zhi li li Shi Quan XI Changchun Institute of Applied Chemistry Academia SinicaChangchun 130022

The molecular interactions between Nd^(3+), Gd^(3+)and Yb^(3+) and DPPC in multibilayer have been studied by Fourier transform infrared spectroscopy. The results show that incorporations of the lanthanide ions considerably shift the gel to liquid-crystal phase transition to higher temperatures. The incorporations of Gd^(3+) and Yb^(3+) obviously reduce the cooperations of the transition. The phosphate region of the spectra indicates that the Ln^(3+) binding to DPPC forms DPPC-Ln^(3+)complex and the Lna^(3+)binding is not dependent on the phase state of DPPC. The C-H stretching region of the spectra suggests that the incorporation of Ln^(3+)increases the conformational order of DPPC both in gel and in liquid-crystal phase as a result of the reduction of gauche rotamers.

关键词： DPPC Nd FOURIER TRANSFORM INFRARED SPECTROSCOPIC STUDY OF EFFECT OF LANTHANIDE IONS ON PHOSPHOliPID MULTIBILAYERS

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荒漠草原灌丛转变过程土壤水分亏缺空间特征及影响因素

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草业学报 2024年第4期33卷 22-34页

作者：赵亚楠王红梅李志丽张振杰陈彦硕苏荣霞宁夏大学林业与草业学院宁夏银川750021 中国科学院地理科学与资源研究所北京100101 宁夏大学西北退化生态系统恢复与重建教育部重点实验室宁夏银川750021

草地灌丛化对生态系统结构、功能与服务产生重要影响,目前已经认识到其对土壤水分的负面影响,但还缺乏其在区域尺度的定量评价及其驱动机制研究。在宁夏荒漠草原选取43块成对样地(即荒漠草地和灌丛地),引入样地土壤水分相对亏缺指数(PCS... 详细信息

草地灌丛化对生态系统结构、功能与服务产生重要影响,目前已经认识到其对土壤水分的负面影响,但还缺乏其在区域尺度的定量评价及其驱动机制研究。在宁夏荒漠草原选取43块成对样地(即荒漠草地和灌丛地),引入样地土壤水分相对亏缺指数(PCSWDI)评价荒漠草原向灌丛转变后土壤水分亏缺空间格局现状及其驱动因子。结果表明:荒漠草原转变为灌丛后0~100 cm和100~200 cm土壤含水量分别显著下降了27.80%和57.92%,0~100 cm灌丛地的PCSWDI显著低于荒漠草地,表明0~100 cm灌丛地目前不存在土壤水分亏缺现象。地统计学分析表明,荒漠草地和灌丛地的0~100 cm土壤水分相对亏缺指数的结构方差比分别为94.73%和95.29%,均属于强空间自相关,主要受结构性因子控制。此外,地理探测器的因子探测发现0~100 cm土壤储水量、坡向和田间持水量是影响灌丛地土壤水分相对亏缺指数的主导因子;交互探测表明,灌丛地0~100 cm土壤水分相对亏缺指数空间分异是多因子共同作用的结果。尽管分析得到0~100 cm灌丛地不存在土壤水分亏缺,但100~200 cm土壤含水量显著下降已经预示了深层土壤水分的消耗。因此,干旱半干旱地区的植被恢复必须考虑其植被承载力和水分阈值,基于自然的解决方案可能是未来植被恢复的主流思路。

关键词：荒漠草原人为灌丛化土壤水分地理探测器样地土壤水分相对亏缺指数

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Fragmentation Reactions of Adduct Ions [M+CH_3CO]^+ of Isomeric Phenylenediamines

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Chinese Chemical Letters 1999年第10期10卷 833-834页

作者： zhi li li Shu Ying liU Long Sheng LONG (1 6 Lab, Shandong Non-Metallic Material Research Institute, Jinan 2500312 Chanchun institute of Applied Chemistry, the Chinese Academy of Sciences, Jilin 1300223 Center of Analysis and Computation, China Pharmaceu 6 Lab. Shandong Non-Metallic Mat. Res. I. Jinan 250031 China Chanchun Inst. of Applied Chemistry Chinese Academy of Sciences Jilin 130022 China Center of Analysis and Computation China Pharmaceutical University Nanjing 210009 China

The dissociation routes of the adduct ions [M+CH3CO]+ formed by ion-moleculereaction of isomeric phenylenediamines with acetyl ion from acetone under chemical ionizationcondition were investigated by using collision-induced dissociation (CID) technique performed ation kinetic energies of 40eV. The adduct ions are intermediate ion-neutral complexes.

关键词： Collision-induced dissociation phenylenediamine ion-neutral complex.

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OsPDR20 is an ABCG metal transporter regulating cadmium accumulation in rice

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Journal of Environmental Sciences 2024年第2期136卷 21-34页

作者： He li Chao li Di Sun zhi Min Yang Department of Biochemistry and Molecular Biology College of Life SciencesNanjing Agricultural UniversityNanjing 210095China

Cadmium(Cd)is a non-essential toxic heavy metal,seriously posing high environmental risks to human health.Digging genetic resources relevant to functional genes is important for understanding the metal absorption and accumulation in crops and bioremediation of Cd-polluted environments.This study investigated a functionally uncharacterized ATP binding cassette transporter G family(ABCG)gene encoding a Pleiotropic Drug Resistance 20(PDR20)type metal transporter which is localized to the plasma membrane of rice.OsPDR20 was transcriptionally expressed in almost all tissues and organs in lifespan and was strongly induced in roots and shoots of young rice under Cd stress.Ectopic expression of OsPDR20 in a yeast mutant ycf1 sensitive to Cd conferred cellular tolerance with less Cd accumulation.Knockdown of OsPDR20 by RNA interference(RNAi)moderately attenuated root/shoot elongation and biomass,with reduced chlorophylls in rice grown under hydroponic medium with 2 and 10μmol/L Cd,but led to more Cd accumulation.A field trial of rice grown in a realistic Cd-contaminated soil(0.40 mg/kg)showed that RNAi plants growth and development were also compromised compared to wild-type(WT),with smaller panicles and lower spikelet fertility but little effect on yield of grains.However,OsPDR20 suppression resulted in unexpectedly higher levels of Cd accumulation in rice straw including lower leaves and culm and grain.These results suggest that OsPDR20 is actively involved in Cd accumulation and homeostasis in rice crops.The increased Cd accumulation in the RNAi plants has the potential application in phytoremediation of Cd-polluted wetland soils.

关键词： Rice ABCG transporter Cadmium accumulation Phytoremediation

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