Poplar catkin:A natural biomaterial for highly specific and efficient enrichment of sialoglycopeptides

作     者：Xiao-Dong Wang Yu-Jie Liu Fen-Jie Li Zhi-Li Li 

作者机构：Department of Biophysics and Structural BiologyInstitute of Basic Medical SciencesChinese Academy of Medical Sciences&School of Basic MedicinePeking Union Medical CollegeBeijing 100005China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2017年第28卷第5期

页      面：1018-1026页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(No.21575164)to Z.L 

主　　题：Sialogycopeptide Poplar catkin Enrichment Natural biomaterial Mass spectrometry 

摘      要：Sialic acids as terminal entities of larger glycans linked to proteins and lipids are involved in multiple different pathological and physiological *** characterisation of sialoglycoconjugates is required to understand their biological ***,a comprehensive sialylation analysis of sialoglycoconjugates has remained *** this study,we employ a natural biomaterial,poplar catkin derived from white poplar tree（Populus tomentosa Carr.）,to develop a novel capturing microtip for selective and efficient enrichment of sialoglycopeptides,without losses of sialic acid residues and water molecules from *** electron microscopy and Fourier-transform infrared spectroscopy analysis,along with Maule and Wiesner staining assays,indicated that the main components on the outer layer of the poplar catkin are syringyl and guaiacyl lignins which play a key role in enriching sialoglycopeptides from complex peptide mixture.