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Lesions of mediodorsal thalamic nucleus reverse abnormal firing of the medial prefrontal cortex neurons in parkinsonian rats

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Neural Regeneration Research 2019年第9期14卷 1635-1642页

作者： Ling-Ling Fan Bo Deng Jun-Bao Yan zhi-hong hu Ai-hong Ren Dong-Wei Yang Department of Physiology Medical College Henan University of Science and Technology

The dysfunction of the medial prefrontal cortex is associated with affective disorders and non-motor features in Parkinson’s ***,the exact role of the mediodorsal thalamic nucleus in the function of the prefrontal cortex remains *** study the possible effects of the mediodorsal thalamic nucleus on the neurological function of the medial prefrontal cortex,a model of Parkinson’s disease was established by injecting 8μg 6-hydroxydopamine into the substantia nigra compacta of *** 1 or 3 weeks,0.3μg ibotenic acid was injected into the mediodorsal thalamic nucleus of the *** 3 or 5 weeks after the initial injury,neuronal discharge in medial prefrontal cortex of rat brain was determined *** numbers of dopamine-positive neurons and tyrosine hydroxylase immunoreactivity in substantia nigra compacta and ventral tegmental area were detected by immunohistochemical *** demonstrated that after injury,the immunoreactivity of dopamine neurons and tyrosine hydroxylase decreased in the substantia nigra compacta and ventral tegmental areas of *** with normal medial prefrontal cortical neurons,at 3 and 5 weeks after substantia nigra compacta injury,the discharge frequency of pyramidal neurons increased and the discharge pattern of these neurons tended to be a burst-discharge,with an increased discharge *** discharge frequency of interneurons decreased and the discharge pattern also tended to be a burst-discharge,but the discharge interval was only higher at 3 *** 3 weeks after the combined lesions,the discharge frequency,discharge pattern and discharge interval were restored to a normal level in pyramidal neurons and interneurons in medial prefrontal *** findings have confirmed that mediodorsal thalamic nucleus is involved in regulating neuronal activities of the medial prefrontal *** changes in the function of the mediodorsal thalamic nucleus may be associated with the abnorma

关键词： nerve regeneration Parkinson's disease mediodorsal thalamic nucleus medial prefrontal cortex pyramidal neurons interneurons discharge neural regeneration

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Quantum Chemistry Study on Local Structure and Properties of Amorphous Fe80P20 Alloy

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Chinese Journal of Chemical Physics 2006年第1期19卷 59-64页

作者： zhi-gang Fang hong-zhi hu Jing-xue Guo Qiu-ju Li School of Chemical Engineering Anshan University ot： Science and Technology Anshan 114044 China

According to the structure features of Fe80P20, A series of clusters Fe4P were designed and focused on studying the stability of local structure, charge distribution and chemical bond, Using the DFT method, energy and structure of Fe4P clusters were optimized and analyzed. The computational results showed that the energy of cluster 1（2） has the lowest energy, and the possibility of its existence in the Fe80P20 is high. Analyzing the transition states among the clusters, it was found that the clusters in the doublet state are more stable than those in the quartet state. The numbers of the Fe-P bond in the clusters play important roles in the cluster stability and electrons transfer properties, The more numbers of Fe-P bonds in the clusters, the higher the cluster stability, and the weaker the ability of P atom to get electron, The number of Fe atoms, which has bonding interactions with the P atom, is direct proportional to the average 3d orbit population of Fe atom. Basing on the orbital population, average magnetic moments of each Fe atom in the Fe4P clusters were calculated, and they are all smaller than that of single metal Fe atom. This suggests that all Fe4P clusters have soft magnetic property and they are expected to be perfect material for preparing soft magnetic apparatus.

关键词： Amorphous Fe80P20 alloy Local structure DFT Cluster

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Application and Prospect of Mixed Reality Technology in Medical Field

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Current Medical Science 2019年第1期39卷 1-6页

作者： hong-zhi hu Xiao-bo FENG Zeng-wu SHAO Mao XIE Song XU Xing-huo WU Zhe-wei YE Department of Orthopaedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Mixed reality(MR)technology is a new digital holographic image technology,which appears in the field of graphics after virtual reality(VR)and augmented reality(AR)technology,a new interdisciplinary *** a new generation of technology,MR has attracted great attention of clinicians in recent *** emergence of MR will bring about revolutionary changes in medical education training,medical research,medical communication,and clinical *** present,MR technology has become the popular frontline information technology for medical *** the popularization of digital technology in the medical field,the development prospects of MR are *** purpose of this review article is to introduce the application of MR technology in the medical field and prospect its trend in the future.

关键词： mixed reality augmented reality virtual reality education surgery

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Virion Proteomics of Large DNA Viruses

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Virologica Sinica 2009年第4期24卷 268-284页

作者： Ran-ran WANG zhi-hong hu hua-lin WANG Fei DENG State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071China

Large DNA viruses normally have complex structures with many of protein components derived from both viral and host origins. The development in proteomics, especially mass spectrometry identification techniques provide powerful tools for analyzing large viruses. In this review, we have summarized the recent achievements on proteomic studies of large DNA viruses, such as herpesvirus, poxvirus, nimavirus and baculoviruse. The proteomics of baculovirus occlusion-derived virions (ODV) were emphasized. Different mass spectrometry techniques used on various baculoviruses were introduced, and the identified structurally associated proteins of baculoviruses are summarized.

关键词： Virion proteomics Large DNA viruses Baculoviruses

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Genetic Modification of Baculovirus Expression Vectors

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Virologica Sinica 2012年第2期27卷 71-82页

作者： Shu-fen Li hua-lin Wang zhi-hong hu Fei Deng State Key Laboratory of Virology and Joint-Lab of Invertebrate Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China

As a protein expression vector, the baculovirus demonstrates many advantages over other vectors. With the development of biotechnology, baculoviral vectors have been genetically modified to facilitate high level expression of heterologous proteins in both insect and mammalian cells. These modifications include utilization of different promoters and signal peptides, deletion or replacement of viral genes for increasing protein secretion, integration of polycistronic expression cassette for producing protein complexes, and baculovirus pseudotyping, promoter accommodation or surface display for enhancing mammalian cell targeting gene delivery. This review summarizes the development and the current state of art of the baculovirus expression system. Further development of baculovirus expression systems will make them even more feasible and accessible for advanced applications.

关键词： Baculovirus Protein expression Promoters Signal peptides Gene delivery

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An Improved Culture System for Virus Isolation and Detection

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Virologica Sinica 2008年第5期23卷 345-351页

作者： Yu-chen XIA zhi-hong hu zhi-juan QIU Zhong-bin MA hua-lin WANG Fei DENG State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071China

Cell culture plays an important role in virology. It provides a platform for the detection and isolation of viruses as well as for the biochemistry and molecular biology based studies of viruses. In the present work, a new system that could permits multiple (different) cell lines to be simultaneously cultured in one dish was developed. In the system, each cell line was cultured in an isolated zone in the same dish or well and the system is therefore called an isolated co-culture system. The usefulness of this novel approach for virus isolation was demonstrated using a model system based on adenovirus.

关键词： Cell culture Isolated co-culture Viral amplification Diagnosis

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Evaluation of Sensitivities and Specificities of SARS-CoV Detection by Real-time Quantitative Reverse Transcription-PCR Assays

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Virologica Sinica 2009年第3期24卷 187-193页

作者： Li-li XU zhi-hong hu hua-lin WANG Xiao HAN Fei DENG State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China

The etiological agent of severe acute respiratory syndrome(SARS) was identified as a new coronavirus,termed SARS-CoV. Establishment of an efficient and sensitive diagnostic system of SARS-CoV genetic materials is crucial for SARS control. In this study,we quantified SARS-CoV mRNAs in both infected cell culture lysate and in supernatant by using Real-time quantitative revere transcription-PCR based on EvaGreenTM dye and Taqman-MGB probes. For extensive evaluation of sensitivities and specificities,13 pairs of primers and 4 probes were designed based on different genes of SARS-CoV. Glyceraldehydes-3-phosphate dehydrogenase(GAPDH) was selected as the internal control gene. Results showed that S-gene-specific PCR was the most sensitive for detection,but because of its sequence variability in the different viral strains,primers and a probe based on the N gene were suitable substitutions. Meanwhile,we found the mRNA concentrations in cell culture lysates were much higher than in cell supernatant and facilited more sensitive detection of the SARS-CoV.

关键词： SARS-CoV Sensitivities Specificities Evaluation

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Production of CCHF Virus-Like Particle by a Baculovirus-Insect Cell Expression System

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Virologica Sinica 2011年第5期26卷 338-346页

作者： Zhao-rui Zhou Man-li Wang Fei Deng Tian-xian Li zhi-hong hu hua-lin Wang State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China

Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family,which is widespread and causes high *** nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S *** this research,the N protein of CCHFV was expressed in insect cells using a recombinant *** an electron microscope,Virus-Like Particles (VLPs) with various size and morphology were observed in cytoplasmic vesicles in the infected ***-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation,which was confirmed by Western blot analysis and immuno-electron microscopy (IEM).

关键词： Crimean-Congo hemorrhagic fever virus (CCHFV) Virus-Like Particle (VLP) Nucleocapsid (N)protein

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DFT Study on Adsorption of Clusters Ni2Fe2P and Ni2Fe2B2

DFT Study on Adsorption of Clusters Ni2Fe2P and Ni2Fe2B2

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中国化学会第九届全国量子化学学术会议

作者： zhi-Gang Fang, hong-zhi hu School of Chemical Engineering, Anshan University of Science and Technology, Anshan, 114044, P. R. China

＜正＞Ternary amorphous alloys Ni-Fe-P and Ni-Fe-B have attracted increasing attention on account of their unique local structure and chemical properties. However, it is very difficult for experimental research owning to their complicated structure, which gives rise to some controversy. And so far, a thorough study in a wide range of composition has been lacking in the world. In a heterogeneous reactions catalyzed by solids, at least one of reactants must be attached in some way for a significant period of time to the surface of the solid catalyst. Therefore, according to the

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Construction of the Bac-to-Bac System of Bombyx mori Nucleopolyhedroviru

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中国病毒学 2007年第3期22卷 218-225页

作者： Jin-shan huANG　 Bi-fang HAO 　 Xiu-lian SUN　 Fei DENG　 hua-lin WANG　 zhi-hong hu State Key Laboratory of Virology and Joint-lab of Invertebrate Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China Northwest A ＆ F University Yangling 712100 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China

To construct the Bac-to-Bac expression system of Bombyx mori nucleopolyhedrovirus(BmNPV),a transfer vector was constructed which contained an Escherichia coli(***)mini-F replicon and a lacZ:attTN7:lacZ cassette within the upstream and downstream regions of the BmNPV polyhedrin *** larvae were cotransfected with wild-type BmNPV genomic DNA and the transfer vector through subcutaneous injection to generate recombinant viruses by homologous recombination in *** genomic DNA of budded viruses extracted from the hemolymph of the transfected larvae was used to transform *** *** bacmids were screened by kanamycin resistance,PCR and restriction enzyme(REN)*** of the bacmid colonies,BmBacJS13,which had similar REN profiles to that of wild-type BmNPV,was selected for further *** investigate the infectivity of BmBacJS13,the polyhedrin gene was introduced into the bacmid and the resultant recombinant(BmBacJS13-ph)was transfected to BmN *** budded viruses were collected from the supernatant of the transfected cells and used for infecting BmN *** curve analysis indicated that BmBacJS13-ph had a similar growth curve to that of wild-type ***-assays indicated that BmBacJS13-ph was also infectious to *** larvae.

关键词：体内表达基因表达家蚕核型多角体病毒 Bac-to-Bac系统基因载体

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