Production of CCHF Virus-Like Particle by a Baculovirus-Insect Cell Expression System

作     者：Zhao-rui Zhou Man-li Wang Fei Deng Tian-xian Li Zhi-hong Hu Hua-lin Wang 

作者机构：State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2011年第26卷第5期

页      面：338-346页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 090403[农学-农药学(可授农学、理学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：The Knowledge Innovation Program of the Chinese Academy of Sciences,Grant No.KSCX2-YW-N-065 the Knowledge Innovation Program of the Chinese Academy of Sciences,Grant No.KSCX2-EW-G-8 National Key Basic Research Program(973Program),Grant No.2010CB530103 National Basic Research Priorities Program of China,Grant No.2007FY210700 

主　　题：Crimean-Congo hemorrhagic fever virus (CCHFV) Virus-Like Particle (VLP) Nucleocapsid (N)protein 

摘      要：Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family,which is widespread and causes high *** nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S *** this research,the N protein of CCHFV was expressed in insect cells using a recombinant *** an electron microscope,Virus-Like Particles (VLPs) with various size and morphology were observed in cytoplasmic vesicles in the infected ***-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation,which was confirmed by Western blot analysis and immuno-electron microscopy (IEM).