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Activity and Tissue Expression of Tyrosine Phosphatase PTP-MEG2

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Chemical Research in Chinese Universities 2011年第2期27卷 287-290页

作者： DONG Hong-bo LI Guo-dong WANG Shao-feng fu xue-qi ZHAO zhi-zhuang Joe Edmond H. Fischer Signal Transduction Laboratory College of Life Science Jilin University Changchun 130012 P. R. China School of Pharmaceutical Science Jilin University Changchun 130021 P. R. China The Second Hospital of Jilin University. Changchun 130041 P. R. China Department of Pathology University of Oklahoma Health Sciences Center Oklahoma City Oklahoma 73104 USA

Protein tyrosine phosphatases（PTPs） are crucial regulators of signal transduction. Among them, PTP-MEG2 is an intracellular enzyme of 593 amino acid residues with a putative lipid-binding domain at the N-terminus. In the present study, we cloned the full-length form of the enzyme and expressed it in E. coli cells as a 6xHis-tagged protein. The majority of the expressed enzyme was found in the inclusion body of E. coli cell extracts. Upon extraction with a buffer containing urea, the recombinant enzyme was purified to near homogeneity on a single Ni-NTA-agarose column. This procedure resulted in the production of over 100 mg of purified recombinant PTP-MEG2 from 1 L E. coli cell culture. The purified protein displayed a single polypeptide band with expected molecular size on SDS-polyacrylamide gel electrophoresis under reducing conditions. Isolated under denatured conditions in urea, the purified enzyme was re-natured by dialyzing against a refolding buffer. The re-natured enzyme effectively dephosphorylated the common PTP substrate para-nitrophenylphosphate with a specific activity of 2000 units/mg. Meanwhile, the denatured enzyme was used to immunize a rabbit to produce antibodies. The resulting anti- serum had extremely high sensitivity and specificity. When used for Western blot analysis, the anti-serum revealed a wide expression of PTP-MEG2 in many tissues of mice. Together, we developed a highly effective way to purify a large amount of PTP-MEG2 and generated highly sensitive antibodies that can specifically detect endogenous expression of the enzyme in tissues.

关键词： Protein tyrosine phosphatase Expression Antibody production Enzyme assay Tissue expression

Global analysis of daily new COVID-19 cases reveals many staticphase countries including the United States potentially with unstoppable epidemic

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World Journal of Clinical Cases 2020年第19期8卷 4431-4442页

作者： Cheng Long Xin-Miao fu zhi-fu fu Department of Orthopaedics Sichuan University West China HospitalChengdu 610041Sichuan ProvinceChina College of Life Sciences Fujian Normal UniversityFuzhou 350117Fujian ProvinceChina Anxi AIER Eye Hospital(AIER EYE Hospital Group) Anxi 362400Fujian ProvinceChina

BACKGROUND The coronavirus disease 2019(COVID-19)pandemic is hitting many *** is hypothesized the epidemic is differentially progressing in different *** To investigate how the COVID-19 epidemic is going on in different countries by analyzing representative *** The status of COVID-19 epidemic in over 60 most affected countries was *** data of daily new cases of each country were collected from *** data of daily tests for the United States,Italy,and South Korea were collected from the Website of One World *** of daily positive COVID-19 tests in the two most affected states of the United States(New York and New Jersey)were collected from the website of the COVID Tracking *** were analyzed using Microcal Origin software with ANOVA algorithm,and significance level was set at a P value of *** The COVID-19 epidemic was differentially progressing in different *** analyses of daily new cases as of April 19,2020 revealed that 61 most affected countries can be classified into four types:Downward(22),upward(20),static-phase(12),and uncertain ones(7).In particular,the 12 static-phase countries including the United States were characterized by largely constant numbers of daily new cases in the past over 14 ***,these static-phase countries were overall significantly lower in testing density(P=0.016)but higher in the level of positive COVID-19 tests than downward countries(P=0.028).These findings suggested that the testing capacity in static-phase countries was lagging behind the spread of the outbreak,i.e.,daily new cases(confirmed)were likely less than daily new infections and the remaining undocumented infections were thus still expanding,resulting in unstoppable *** Increasing the testing capacity and/or reducing the COVID-19 transmission are urgently needed to stop the potentially unstoppable,severing crisis in static-phase countries.

关键词： COVID-19 SARS-CoV-2 Coronavirus Pandemic Testing density

Must pilots permanently quit flying career after treatment for colorectal cancer?-Medical waiver for Air Force pilots with colorectal cancer: Three case reports

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World Journal of Clinical Cases 2020年第4期8卷 790-797页

作者： Guo-Li Gu fu-Xiao Duan zhi Zhang xue-Ming Wei Li Cui Bo Zhang Department of General Surgery Air Force Medical CenterPLABeijing 100142China Department of General Surgery the General Hospital of Northern Theater Command PLAShenyang 110016Liaoning ProvinceChina Department of Aviation Diseases Air Force Medical CenterPLABeijing 100142China Department of Medical Research Air Force Medical CenterPLABeijing 100142China

BACKGROUND Colorectal cancer(CRC)could seriously threaten the physical and mental health of *** they end their flying after treatment of CRC?With this study,we investigated the possibility of a gradual medical waiver for such pilots to fly aircrafts again after treatment of *** SUMMARY We analyzed the medical waiver and clinical data of 3 pilots with CRC,who had accepted the treatment at the Department of General Surgery,Air Force Medical Center(formerly,Air Force General Hospital)between 2013 and *** 3 cases underwent a series of comprehensive treatment courses,including radical resection of CRC,sequential radiotherapy,and *** follow-up results were *** passing through the high-risk period of recurrence and metastasis of CRC,they all were given a medical waiver for flying *** observation showed that their flying operations were *** The CRC treatment shall follow the guidelines for diagnosis and treatment and should simultaneously protect the combating capabilities of pilots as much as *** is safe for pilots with CRC,who are continuously monitored under medical observation after passing through the high-risk period of recurrence and metastasis,to undertake military flight missions again.

关键词： Colorectal cancer Pilot Waiver Treatment Military medicine Aeromedicine Case report

Some Developments in Nielsen Fixed Point Theory

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Acta Mathematica Sinica,English Series 2018年第1期34卷 91-102页

作者： Bo Ju JIANG xue zhi ZHAO School of Mathematical Sciences Peking University Department of Mathematics Capital Normal University

We give a brief survey of some developments in Nielsen fixed point theory. After a look at early history and a digress to various generalizations, we confine ourselves to several topics on fixed points of self-maps on manifolds and polyhedra. Special attention is paid to connections with geometric group theory and dynamics, as well as some formal approaches.

关键词： Fixed points Nielsen theory

An Improved F-Expansion Method and Its Application to Coupled Drinfel'd-SokolovWilson Equation

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Communications in Theoretical Physics 2008年第8期50卷 309-314页

作者： ZHAO xue-Qin zhi Hong-Yan Department of Mathematics Qufu Normal University Shandong Rizhao 276826 China Department of Applied Mathematics Dalian University of Technology Dalian 116024 China

With the aid of computerized symbolic computation, an improved F-expansion method is presented to uniformly construct more new exact doubly periodic solutions in terms of rational formal Jscobi elliptic function of nonlinear partial differential equations （NPDFs）. The coupled Drinfel＇d-Sokolov-Wilson equation is chosen to illustrate the method. As a result, we can successfully obtain abundant new doubly periodic solutions without calculating various Jacobi elliptic functions. In the limit cases, the rational solitary wave solutions and trigonometric function solutions are obtained as well.

关键词： Jacobi elliptic function doubly periodic solution rational solitary wave solution

ON NEWTON-HSS METHODS FOR SYSTEMS OF NONLINEAR EQUATIONS WITH POSITIVE-DEFINITE JACOBIAN MATRICES

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Journal of Computational Mathematics 2010年第2期28卷 235-260页

作者： Zhong-zhi Bai xue-Ping Guo LSEC ICMSEC Academy of Mathematics and Systems Science Chinese Academy of Sciences Beijing 100190 China Department of Mathematics East China Normal University Shanghai 200062 China

The Hermitian and skew-Hermitian splitting （HSS） method is an unconditionally convergent iteration method for solving large sparse non-Hermitian positive definite system of linear equations. By making use of the HSS iteration as the inner solver for the Newton method, we establish a class of Newton-HSS methods for solving large sparse systems of nonlinear equations with positive definite Jacobian matrices at the solution points. For this class of inexact Newton methods, two types of local convergence theorems are proved under proper conditions, and numerical results are given to examine their feasibility and effectiveness. In addition, the advantages of the Newton-HSS methods over the Newton-USOR, the Newton-GMRES and the Newton-GCG methods are shown through solving systems of nonlinear equations arising from the finite difference discretization of a two-dimensional convection-diffusion equation perturbed by a nonlinear term. The numerical implemen- tations also show that as preconditioners for the Newton-GMRES and the Newton-GCG methods the HSS iteration outperforms the USOR iteration in both computing time and iteration step.

关键词： Systems of nonlinear equations HSS iteration method Newton method Local convergence.

A New General Algebraic Method and Its Application to Shallow Long Wave Approximate Equations

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Communications in Theoretical Physics 2007年第5X期48卷 781-786页

作者： ZHAO xue-Qin zhi Hong-Yan Department of Mathematics Qufu Normal University Rizhao 276826 China Department of Applied Mathematics Dalian University of Technology Dalian 116024 China

A new general algebraic method is presented to uniformly construct a series of exact solutions for nonlinear evolution equations （NLEEs）. For illustration, we apply the new method to shallow long wave approximate equations and successfully obtain abundant new exact solutions, which include rational solitary wave solutions and rational triangular periodic wave solutions. The method is straightforward and concise, and it can also be applied to other nonlinear evolution equations in mathematical physics.

关键词： new general algebraic method nonlinear evolution equations solitary wave solutions

Sequential intraventricular injection of tigecycline and polymyxin B in the treatment of intracranial Acinetobacter baumannii infection after trauma: a case report and review of the literature

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Military Medical Research 2020年第4期7卷 497-502页

作者： Li Zhong xue-zhi Shi Lei Su zhi-Feng Liu Second Clinical College of Guangzhou University of Chinese Medicine Guangzhou 510515China Department of Critical Care Medicine the First Affiliated Hospital of Guizhou University of Chinese MedicineGuiyang 550001China Department of Critical Care Medicine General Hospital of Southern Theater Command of PLAGuangzhou 510010China Key Laboratory of Hot Zone Trauma Care and Tissue Repair of PLA General Hospital of Southern Theater Command of PLAGuangzhou 510010China

Background:Intracranial infection after craniotomy is one of the most serious postoperative complications,especially multidrug-resistant(MDR)or extensively drug-resistant(XDR)bacterial meningitis,and strongly affects the prognosis of *** treatment experience regarding these infections is *** presentation:We report a case of severe intracranial infection of XDR Acinetobacter baumannii(***)that was treated by intravenous(IV)injection,sequential intraventricular(IVT)injection of tigecycline and polymyxin B,and other anti-infective *** results were obtained,and the patient was eventually discharged from the *** case is characterized by intracranial ***:The polymyxin B IV+IVT pathway is an ideal treatment strategy for XDR *** tigecycline IVT pathway is also a safe treatment option.

关键词： Intraventricular injection of tigecycline Polymyxin B Intracranial infection Acinetobacter baumannii

Mixtures of Semiparametric Varying Coefficient Models for Longitudinal Data with Nonignorable Dropout

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Acta Mathematicae Applicatae Sinica 2010年第1期26卷 125-132页

作者： zhi-qiang Li Liu-gen xue College of Sciences Beijing University of Chemical Technology Beijing 100029 China College of Applied Sciences Beijing University of Technology Beijing 100022 China

Informative dropout often arise in longitudinal data. In this paper we propose a mixture model in which the responses follow a semiparametric varying coefficient random effects model and some of the regression coefficients depend on the dropout time in a non-parametric way. The local linear version of the profile-kernel method is used to estimate the parameters of the model. The proposed estimators are shown to be consistent and asymptotically normal, and the finite performance of the estimators is evaluated by numerical simulation.

关键词： Nonignorable dropout, Estimating equation, Profile-kernel, Local linear estimation, Longitudinal data Semiparametric varying coefficient