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Journal of Integrative Plant Biology 2005年第11期47卷 1345-1351页

作者： zhi-yong xiong yong LIU yong-Gang HE Yun-Chun SONG Ke-Xiu LI Guan-Yuan HE China-UK HUST-RRes （Rothamsted Reseach UK） Genetic Engineering and Genomics Joint Laboratory Huazhong University of Science and Technology （HUST） Wuhan 430074 China Key Laboratory of the Ministry of Education for Plant Developmental Biology Wuhan University Wuhan 430072 China

Knobs are blocks of heterochromatin present on chromosomes of maize （Zea mays L.） and its relatives that have effects on the frequency of genetic recombination, as well as on chromosome behavior. Knob heterozygosity and instability in six maize inbred lines and one Z. diploperennis Iltis Doebley line were investigated using the fluorescence in situ hybridization （FISH） technique with knob-associated tandem repeats （180 bp and 350 bp （TR- 1）） as probes. Signals of seven heterozygous knobs containing 180- bp repeats and of one heterozygous knob containing TR- 1 were captured in chromosomes of all materials tested according to the results of FISH, which demonstrates that the 180-bp repeat is the main contributor to knob heterozygosity compared with the TR- 1 element. In addition, one target cell with two TR- 1 signals on one homolog of chromosome 2L, which was different from the normal cells in the maize inbred line GB57, was observed, suggesting knob duplication and an instability phenomenon in the maize genome.

关键词： fluorescence in situ hybridization knob repetitive DNA sequence Zea diploperennis Z. mays.

Characterization of Interspecific Hybrids Between Oryza sativa L, and Three Wild Rice Species of China by Genomic In Situ Hybridization

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Journal of Integrative Plant Biology 2006年第9期48卷 1077-1083页

作者： Guang-Xuan Tan zhi-yong xiong Hua-Jun Jin Gang Li Li-Li Zhu Li-Hui Shu Guang-Cun He Key Laboratory of the Ministry of Education for Plant Development Biology Wuhan University College of Life Sciences Wuhan 430072 China Zhoukou Normal University Zhoukou 466001 China

In the genus Oryza, interspecific hybrids are useful bridges for transferring the desired genes from wild species to cultivated rice （Oryza sativa L.）. In the present study, hybrids between O. sativa （AA genome） and three Chinese wild rices, namely O. rufipogon （AA genome）, O. officinalis （CC genome）, and O. meyeriana （GG genome）, were produced. Agricultural traits of the F1 hybrids surveyed were intermediate between their parents and appreciably resembled wild rice parents. Except for the O. sativa × O. rufipogon hybrid, the other F1 hybrids were completely sterile. Genomic in situ hybridization （GISH） was used for hybrid verification. Wild rice genomic DNAs were used as probes and cultivated rice DNA was used as a block. With the exception of O. rufipogon chromosomes, this method distinguished the other two wild rice and cultivated rice chromosomes at the stage of mitotic metaphase with different blocking ratios. The results suggest that a more distant phylogenetic relationship exists between O. meyeriana and O. sativa and that O. rufipogon and O. sativa share a high degree of sequence homology. The average mitotic chromosome length of O. officinalis and O. meyeriana was 1.25- and 1.51-fold that of O. sativa, respectively. 4＇,6＇-Diamidino- 2-phenylindole staining showed that the chromosomes of O. officinalis and O. meyeriana harbored more heterochromatin, suggesting that the C and G genomes were amplified with repetitive sequences compared with the A genome. Although chromocenters formed by chromatin compaction were detected with wild rice-specific signals corresponding to the C and G genomes in discrete domains of the F1 hybrid interphase nuclei, the size and number of O. meyeriana chromocenters were bigger and greater than those of O. officinalis. The present results provide an important understanding of the genomic relationships and a tool for the transfer of useful genes from three native wild rice species in China to cultivars.

关键词： Chinese wild rice species interspecific hybrids genomic in situ hybridization (GISH) Oryza sativa

Decreased expression of ApoF associates with poor prognosis in human hepatocellular carcinoma

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Gastroenterology Report 2019年第5期7卷 354-360,I0002页

作者： Ya-Bin Wang Bo-Xuan Zhou Yun-Biao Ling zhi-yong xiong Rui-Xi Li Yue-Si Zhong Ming-Xing Xu Yi Lu Hao Liang Gui-Hua Chen zhi-Cheng Yao Mei-Hai Deng Department of Liver Transplantation Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdongP.R.China Department of Hepatobiliary Surgery Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdongP.R.China Department of General Surgery The Lingnan Hospital of Sun Yat-sen UniversityGuangzhouGuangdongP.R.China

Background:Hepatocellular carcinoma(HCC)is frequently associated withmetabolism *** evidence has demonstrated the crucial role of lipidmetabolismin HCC *** function of apolipoprotein F(ApoF),a lipid transfer inhibitor protein,in HCC is incompletely *** aimed to evaluate the functional role of ApoF in HCC in this ***:We used quantitative reverse-transcription polymerase chain reaction(qRT-PCR)to detect ApoF mRNA expression in HCC tissues and hepatoma cell lines(SMMC-7721,HepG2,and Huh7).Immunohistochemistry was performed to detect the expression of ApoF in HCC *** associations between ApoF expression and clinicopathological features as well as HCC prognosis were *** effect of ApoF on cellular proliferation and growth of SMMC-7721 and Huh7 cells was examined in vitro and in ***:ApoF expression was significantly down-regulated at both mRNA and protein levels in HCC tissues as compared with adjacent *** SMMC-7721 and Huh7 HCC cells,ApoF overexpression inhibited cell proliferation and *** a xenograft nude mouse model,ApoF overexpression effectively controlled HCC ***–Meier analysis results showed that the recurrence-free survival rate of HCC patients with low ApoF expression was significantly lower than that of other HCC *** ApoF expression was associated with several clinicopathological features such as liver cirrhosis,Barcelona Clinic Liver Cancer stage and tumor-node-metastasis ***:ApoF expression was down-regulated in HCC,which was associated with low recurrence-free survival *** may serve as a tumor suppressor in HCC and be a potential application for the treatment of this disease.

关键词： hepatocellular carcinoma biomarkers apolipoprotein F prognosis

Cytogenetic comparisons between A and G genomes in Oryza using genomic in situ hybridization

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Cell Research 2006年第3期16卷 260-266页

作者： zhi yong xiong~(1,2) Guang Xuan Tan~1 Guang Yuan He~2 Guang Cun He~1 Yun Chun Song~11 Key Laboratoty of the Ministry of Education for Plant Developmental Biology,College of Life Sciences,Wuhan University,Wuhan 430072,China.2 China-UK HUST-R Res Genetic Engineering and Genomics Joint Laboratory,Huazhong University of Science and Technology,Wuhan 430074,China Key Laboratory of the Ministry of Education for Plant Developmental Biology College of Life Sciences Wuhan University Wuhan China China-UK HUST-R Res Genetic Engineering and Genomics Joint Laboratory Huazhong University of Science and Technology Wuhan China

The genomic structures ofOryza sativa （A genome） and *** （G genome） were comparatively studied usingbieolor genomic in situ hybridization （GISH）.GISH was clearly able to discriminate between the chromosomes of *** and *** in the interspecific F1 hybrids without blocking DNA,and co-hybridization was hardly *** average mitotic chromosome length of *** was found to be 1.69 times that of ***.A comparison of4,6-diamidino-2-phenylindole staining showed that the chromosomes of *** were more extensively labelled,suggesting that the G genome is amplified with more repetitive sequences than the A *** interphase nuclei,9-12chromocenters were normally detected and nearly all the chromocenters constituted the G genome-specific *** larger chromocenters formed by chromatin compaction corresponding to the G genome were detected in the hybridcompared with its *** pachytene of the F1 hybrid,most chromosomes of A and G did not synapse eachother except tot 1-2 chromosomes paired at the end of their *** meiotic metaphase I,three types of chromosomalassociations,i.e.O,sativa-O,sotiva（A-A）,***-O,meyeriana （A-G） and ***-O,meyeriana （G-G）,wereobserved in the F1 *** A-G chromosome pairing configurations included bivalcnts and *** resultsprovided a foundation toward studying genome organization and evolution of ***.

关键词： Oryza sativa Oryza meyeriana 45S rDNA genomic in situ hybridization

The emerging role of nitric oxide in the synaptic dysfunction of vascular dementia

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Neural Regeneration Research 2025年第2期20卷 402-415页

作者： Xiaorong Zhang zhiying Chen Yinyi xiong Qin Zhou Ling-Qiang Zhu Dan Liu Department of Pathology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Jiujiang Clinical Precision Medicine Research Center JiujiangJiangxi ProvinceChina Center for Cognitive Science and Transdisciplinary Studies Jiujiang UniversityJiangxi ProvinceChina Department of Neurology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Department of Rehabilitation Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Department of Pathophysiology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

With an increase in global aging,the number of people affected by cerebrovascular diseases is also increasing,and the incidence of vascular dementia-closely related to cerebrovascular risk-is increasing at an epidemic ***,few therapeutic options exist that can markedly improve the cognitive impairment and prognosis of vascular dementia *** in Alzheimer’s disease and other neurological disorders,synaptic dysfunction is recognized as the main reason for cognitive *** oxide is one of the ubiquitous gaseous cellular messengers involved in multiple physiological and pathological processes of the central nervous ***,nitric oxide has been implicated in regulating synaptic plasticity and plays an important role in the pathogenesis of vascular *** review introduces in detail the emerging role of nitric oxide in physiological and pathological states of vascular dementia and summarizes the diverse effects of nitric oxide on different aspects of synaptic dysfunction,neuroinflammation,oxidative stress,and blood-brain barrier dysfunction that underlie the progress of vascular ***,we propose that targeting the nitric oxide-sGC-cGMP pathway using certain specific approaches may provide a novel therapeutic strategy for vascular dementia.

关键词： endoplasmic reticulum stress endothelial nitric oxide synthase gene therapy nitric oxide NO-sGC-cGMP pathway synaptic dysfunction vascular dementia

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Three-dimensional fracture of UO2 ceramic pellets by phase field modeling

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 169-180页

作者： Wei xiong Xuan Ye Hongzhang Cheng Xiaoming Liu Institute of Nuclear and New Energy Technology Collaborative Innovation Center of Advanced Nuclear Energy Technology Key Laboratory of Advanced Reactor Engineering and Safety of Ministry of Education Tsinghua University State Key Laboratory of Nonlinear Mechanics Institute of Mechanics Chinese Academy of Sciences School of Engineering Science University of Chinese Academy of Sciences

Fracture is the primary failure type for the UO2ceramic pellets,which are operated under irradiation and thermal processes,and physically confined by the *** this work,by combining closely coupled physical processes of heat conduction,heat transfer,and mechanical deformation with the cohesive phase field framework,we proposed a high-precision modeling of quasistatic cracking of three-dimensional （3D） UO2ceramic fuel pellets under high temperature and *** simulation results agree well with the experimental results,indicating that the proposed method has the potential to capture 3D crack modes in the interested time ***,we studied the relation of the 3D fracture patterns of fuel pellets with the peak power *** was found that the power level plays a critical role in determining the competition between radial and circumferential cracks,as well as transverse penetrating cracks.

关键词： phase field method nuclear fuel pellet coupled thermo-mechanical model thermal fracture

Influence of introducing Zr,Ti,Nb and Ce elements on externally solidified crystals and mechanical properties of high-pressure die-casting Al–Si alloy

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 147-153页

作者： Junjie Li Wenbo Yu Zhenyu Sun Weichen Zheng Liangwei Zhang Yanling Xue Wenning Liu Shoumei xiong Center of Materials Science and Engineering School of Mechanical and Electronic Control EngineeringBeijing Jiaotong UniversityBeijing 100044China Traffic&Transportation Engineering Central South UniversityChangsha 410075China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

High pressure die casting(HPDC)AlSi10Mn Mg alloy castings are widely used in the automobile *** can optimize the mechanical properties of castings through heat treatment,while the release of thermal stress arouses the deformation of large integrated ***,the development of non-heat treatment Al alloys is becoming the hot *** addition,HPDC contains externally solidified crystals(ESCs),which are detrimental to the mechanical properties of *** achieve high strength and toughness of non-heat treatment die-casting Al-Si alloy,we used AlSi9Mn alloy as matrix with the introduction of Zr,Ti,Nb,and *** influences on ESCs and mechanical properties were systematically investigated through three-dimensional reconstruction and thermodynamic *** results reveal that the addition of Ti increased ESCs'size and porosity,while the introduction of Nb refined ESCs and decreased ***,large-sized Al_3(Zr,Ti)phases formed and degraded the mechanical *** introduction of Ce resulted in the poisoning effect and reduced mechanical properties.

关键词： aluminium alloy high-pressure die-casting externally solidified crystals non-heat treatment

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Unraveling the mechanism of action of Shangxia Liangji formula for treating insomnia:a metabolomics and network pharmacology approach

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Traditional Medicine Research 2025年第2期10卷 16-29页

作者： Xia-Jie Quan Hao Liang yong-Hong Tang Li Jiang xiong-Ying Ji Feng-Ying Zhang Ping Zhang Bo Ouyang Department of Information The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China Department of Neurology The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China Department of Gastroscopy The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China Department of Traditional Chinese Medicine The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China

Background:Insomnia is a prevalent clinical condition and Shangxia Liangji formula(SXLJF)is a well-established method of ***,the specific mechanism of action of SXLJF remains ***:The mouse model of insomnia was established by intraperitoneal injection of ***-two mice were randomly divided into a negative control group,model group,SXLJF group(18.72 g/kg/day),and positive control group(diazepam,2 mg/kg)and treated with the corresponding drugs for 7 consecutive *** open field test and pentobarbital-induced sleeping test were ***-MS-based untargeted metabolomics and network pharmacology were applied to explore the potential targets of SXLJF for treating ***,key targets were validated using ***:Behavioral tests demonstrated that SXLJF reduced the total distance,average velocity,central distance,and sleep latency,and prolonged sleep *** and network pharmacology revealed potential targets,signaling pathways,metabolic pathways,and metabolites associated with the anti-insomnia effects of ***,tyrosine hydroxylase(TH)and tyrosine metabolism emerged as crucial metabolic pathways and targets,***-qPCR results supported the role of TH in the mechanism of SXLJF in treating ***:In conclusion,TH and tyrosine metabolism may represent significant targets and pathways for SXLJF in treating insomnia.

关键词： Shangxia Liangji formula insomnia metabolomics network pharmacology tyrosine hydroxylase tyrosine metabolism

Ethnobotany and diversity of medicinal plants used by the Buyi in eastern Yunnan, China

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Plant Diversity 2020年第6期42卷 401-414页

作者： yong xiong Xueyi Sui Selena Ahmed zhi Wang Chunlin Long College of Life and Environmental Sciences Minzu University of ChinaBeijing100081China Key Laboratory of Ethnomedicine(Minzu University of China) Ministry of EducationBeijing100081China Tabaco Breeding and Biotechnology Center Yunnan Academy of Tabaco Agricultural SciencesKunming650021China Sustainable Food and Bioenergy Systems Program Department of Health and Human DevelopmentMontana State UniversityBozemanMT59717USA School of Pharmacy Hunan University of Chinese MedicineChangsha410208China

The Buyi are a socio-linguistic group in Yunnan Province of southwest China that have a long history of using medicinal plants as part of their indigenous medical *** the limited written documentation of the Buyi indigenous medical system,the objective of this paper is to document the medicinal plants of the Buyi and associated traditional knowledge and *** research was conducted in four villages in Lubuge Township of Luoping County in Yunnan Province using ethnobotanical methodologies including participatory observation,semi-structured interviews,key informant interviews,and focus group discussions to elicit information on medicinal *** total,120 informants(including 15 key informants who are healers)were *** study found that a total of 121 medicinal plant species belonging to 64 families are used by the Buyi including by local healers to treat different *** the medicinal plants recorded in this study,56 species(46%)have not previously been documented in the scientific literature as having medicinal value,highlighting the pressing need for ethnobotanical documentation in indigenous *** most frequently used medicinal part was the leaf(24.9%of documented plants),and the most common preparation method was decoction(62.8%of medicinal).Medicinal plants were mainly used to treat rheumatism(12.4%of plants),trauma and injuries(9.6%).The documented plants are also used for other non-medicinal purposes including food,fodder,fencing,and *** addition,35 of the medicinal plants are considered poisonous and are used by local Buyi healers for *** traditional Buyi beliefs and practices associated with the documented medicinal plants likely contributes to their conservation in the environments and around Buyi *** study further highlights that ethnomedicinal knowledge of the Buyi is at risk of disappearing due to increased introduction and use of modern medicine in Buyi communities,live

关键词： Ethnobotany Indigenous medical systems Ethnobotanical knowledge Buyi Medicinal plants

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On the selection of solutions for mutation in differential evolution

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Frontiers of Computer Science 2018年第2期12卷 297-315页

作者： yong WANG zhi-Zhong LIU Jianbin LI Han-xiong LI Jiahai WANG School of Information Science and Engineering Central South University Changsha 410083 China School of Computer Science and Informatics De Montfort University Leicester LE1 9BH UK Institute of Information Security and Big Data Central South University Changsha 410083 China Department of Systems Engineering and Engineering Management City University of Hong Kong Hong Kong China State Key Laboratory of High Performance Complex Manufacturing Central South University Changsha 410083 China School of Data and Computer Science Sun Yat-sen University Guangzhou 510006 China

Differential evolution （DE） is a kind of evolutionary algorithms, which is suitable for solving complex optimization problems. Mutation is a crucial step in DE that generates new solutions from old ones. It was argued and has been commonly adopted in DE that the solutions selected for mutation should have mutually different indices. This restrained condition, however, has not been verified either theoretically or empirically yet. In this paper, we empirically investigate the selection of solutions for mutation in DE. From the observation of the extensive experiments, we suggest that the restrained condition could be relaxed for some classical DE versions as well as some advanced DE variants. Moreover, relaxing the restrained condition may also be useful in designing better future DE algorithms.

关键词： differential evolution mutation the selection of solutions for mutation evolutionary algorithms