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Effects of n-3 polyunsaturated fatty acids from seal oils on nonalcoholic fatty liver disease associated with hyperlipidemia

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World Journal of Gastroenterology 2008年第41期14卷 6395-6400页

作者： Feng-Shang Zhu Su Liu Xi-Mei Chen zhi-gang huang Dong-Wei Zhang Digestive Disease Institute of Tongji University Shanghai 200065 China Department of Gastroenterology Center Hospital of Shanghai Zhabei District Shanghai 200072 China

AIM: To investigate the efficacy and safety of n-3 polyunsaturated fatty acids (PUFA) from seal oils for patients with nonalcoholic fatty liver disease (NAFLD) associated with hyperlipidemia. METHODS: One hundred and forty-four patients with NAFLD associated with hyperlipidemia were included in the 24-wk, randomized, controlled trial. The patients were randomized into two groups. Group A (n = 72) received recommended diet and 2 g n-3 PUFA from seal oils, three times a day. Group B (n = 72) received recommended diet and 2 g placebo, three times a day. Primary endpoints were fatty liver assessed by symptom scores, liver alanine aminotransferase (ALT) and serum lipid levels after 8, 12, 16, and 24 wk. Hepatic fat inf iltration was detected by ultrasonography at weeks 12 and 24 after treatment. RESULTS: A total of 134 patients (66 in group A, 68 in group B) were included in the study except for 10 patients who were excluded from the study. After 24 wk of treatment, no change was observed in body weight, fasting blood glucose (FBG), renal function and blood cells of these patients. Total symptom scores, ALT and triglyceride (TG) levels decreased more significantly in group A than in group B (P < 0.05). As expected, there was a tendency toward improvement in aspartate aminotransferase (AST), γ-glutamyltranspeptidase (GGT), and total cholesterol (TCHO) and high- density lipoprotein (HDL) cholesterol levels (P < 0.05) after administration in the two groups. However, no significant differences were found between the two groups. The values of low-density lipoprotein (LDL) were significantly improved in group A (P < 0.05), but no significant change was found in group B at different time points and after a 24-wk treatment. After treatment, complete fatty liver regression was observed in 19.70% (13/66) of the patients, and an overall reduction was found in 53.03% (35/66) of the patients in group A. In contrast, in group B, only f ive patients (7.35%, 5/68) achieved complete fatt

关键词： Nonalcoholic fatty liver disease Polyunsaturated fatty acids Seal oil Hyperlipidemia, Therapy

Endogenous hydrogen sulfide and ERK1/2-STAT3 signaling pathway may participate in the association between homocysteine and hypertension

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Journal of Geriatric Cardiology 2019年第11期16卷 822-834页

作者： Lin SHI Xiao-Yun LIU zhi-gang huang zhi-Yi MA Yang XI Lu-Yan WANG Ning-Ling SUN Hypertension Research Laboratory Department of CardiologyPeking University People’s HospitalBeijingChina Emergency Department Peking University Shenzhen HospitalShenzhenChina

Background Homocysteine(Hcy)is a risk factor for hypertension,although the mechanisms are poorly *** We first explored the relationship between Hcy levels and blood pressure(BP)by analyzing the clinical data of primary hypertensive patients admitted to our ***,we explored a rat model to study the effect of Hcy on blood pressure and the role of *** hyperhomocysteinemia(HHcy)rat model was induced to explore the effect of Hcy on blood pressure and the possible *** carried out tissue histology,extraction and examination of RNA and ***,we conducted cell experiments to determine a likely mechanism through renin-angiotensin-aldosterone system(RAAS)and extracellular signal-regulated kinase 1/2(ERK1/2)signaling *** In primary hypertensive inpatients with HHcy,blood pressure was significantly higher as compared with inpatient counterparts lacking *** the rat model,blood pressure of the Wistar rats was significantly increased with increases in serum Hcy levels and decreased after folate *** converting enzyme 1(ACE1)expression in the Wistar Hcy group was enhanced comparing to controls,but was decreased in the Wistar folate *** II receptor type 1(AGTR1)levels in the kidney tissue increased in the Wistar folate *** serum H2S and kidney cystathionineγ-lyase decreased with elevated levels of serum *** vitro,increased concentrations and treatment times for Hcy were associated with increased expression of collagen type 1 and *** dose and time dependent response was also observed for p-STAT3 and p-ERK1/2 *** Endogenous H2S might mediate the process of altered blood pressure in response to changes in serum Hcy levels,in a process that is partly dependent on activated RAAS and ERK1/2-STAT3 signaling pathway.

关键词： Angiotensin converting enzyme 1 Blood pressure ERK1/2-STAT3 signaling pathway Homocysteine Hydrogen sulfide

Mutation of cytotoxin-associated gene A affects expressions of antioxidant proteins of Helicobacter pylori

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World Journal of Gastroenterology 2009年第5期15卷 599-606页

作者： zhi-gang huang Guang-Cai Duan Qing-Tang Fan Wei-Dong Zhang Chun-Hua Song Xue-Yong huang Rong-Guang Zhang Department of Epidemiology College of Public HealthZhengzhou University Zhengzhou 450001 Henan ProvinceChina Department of Epidemiology School of Public Health Guangdong Medical College Dongguan 523808Guangdong Province China

AIM: To determine if disruption of the cagA gene of Helicobacter pylori (H pylori) has an effect on the expression of other proteins at proteome level. METHODS: Construction of a cagA knock out mutant Hp27 _△cagA (cagA -) via homologous recombination with the wild-type strain Hp27 (cagA+) as a recipient was performed. The method of sonication-urea-CHAPS-DTT was employed to extract bacterial proteins from both strains. Soluble proteins were analyzed by two-dimensional electrophoresis (2-DE). Images of 2-DE gels were digitalized and analyzed. Only spots that had a statistical signif icance in differential expression were selected and analyzed by matrix-assisted laser desorption/ionizationtime of flight mass spectrometry (MALDI-TOF-MS). Biological information was used to search protein database and identify the biological function of proteins. RESULTS: The proteome expressions between wild-type strain and isogenic mutant with the cagA gene knocked-out were compared. Five protein spots with high abundance in bacteria proteins of wild-type strains, down-regulated or absently expressed in bacteria proteins of mutants, were identified and analyzed. From a quantitative point of view, the identified proteins are related to the cagA gene and important antioxidant proteins of H pylori , including alkyl hydroperoxide reductase (Ahp), superoxide dismutase (SOD) and modulator of drug activity (Mda66), respectively, suggesting that cagA is important to maintain the normal activity of antioxidative stress and ensure H pylori persistent colonization in the host. CONCLUSION: cagA gene is relevant to the expressions of antioxidant proteins of H pylori, which may be a novel mechanism involved in H pylori cagA pathogenesis.

关键词： Helicobacter pylorr, Cytotoxin-associatedgene A Knock-out Antioxidant protein Two-dimensional electrophoresis

Combined detection of plasma GATA5 and SFRP2 methylation is a valid noninvasive biomarker for colorectal cancer and adenomas

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World Journal of Gastroenterology 2015年第9期21卷 2629-2637页

作者： Xie Zhang Yu-Fei Song Hong-Na Lu Dan-Ping Wang Xue-Song Zhang Shi-Liang huang Bei-Lei Sun zhi-gang huang Department of Gastroenterology Ningbo Medical Treatment Center Li Huili Hospital School of Medicine Ningbo University Department of Gastroenterology Zhejiang Provincial People’s Hospital

AIM:To investigate GATA5,SFRP2,and ITGA4 methylation in plasma DNA as noninvasive biomarkers for colorectal cancer(CRC) or ***:There were 57 CRC patients,30 adenomas patients,and 47 control patients enrolled in this ***-specific polymerase chain reaction was used to determine the promoter methylation status of GATA5,SFRP2,and ITGA4 genes in plasma DNA,and their association with clinical outcome in *** predictive ability of GATA5,SFRP2,and ITGA4 methylation,individually or in combination,to detect CRC or adenomas was further ***:Hypermethylated GATA5 was detected in plasma in 61.4%(35/57) of CRC cases,43.33%(13/30) of adenoma cases,and 21.28%(10/47) of control *** hypermethylation of SFRP2 was detected in 54.39%(31/57),40.00%(12/30),and 27.66%(13/47) in plasma samples from CRC,adenomas,and controls,***4 methylation was detected in 36.84%(21/57) of plasma samples of CRC patients and in 30.00%(9/30) of plasma samples from patients with colorectal adenomas,and the specificity of this individual biomarker was 80.85%(9/47).Moreover,GATA5 methylation in the plasma was significantly correlated with larger tumor size(P =0.019),differentiation status(P =0.038),TNM stage(P =0.008),and lymph node metastasis(P =0.008).SFRP2 and ITGA4 methylation in plasma significantly correlated with differentiation status(SFRP2,P =0.012; ITGA4,P =0.007),TNM stage(SFRP2,P =0.034; ITGA4,P =0.021),and lymph node metastasis(SFRP2,P =0.034; ITGA4,P =0.021).From the perspective of predictive power and cost-performance,using GATA5 and SFRP2 together as methylation markers seemed the most favorable predictor for CRC(OR =8.06;95%CI:2.54-25.5; P < 0.01) and adenomas(OR =3.35; 95%CI:1.29-8.71; P =0.012).CONCLUSION:A combination of GATA5 and SFRP2 methylation could be promising as a marker for the detection and diagnosis of CRC and adenomas.

关键词： Colorectal cancer GATA binding protein 5 Secreted

Author correction to‘Amino-functionalized poloxamer 407 with both mucoadhesive and thermosensitive properties:Preparation,characterization and application in vaginal drug delivery system'[Acta Pharm Sin B 7(2017)593-602]

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Acta Pharmaceutica Sinica B 2023年第9期13卷 3961-3962页

作者： Li-qian Ci zhi-gang huang Yu Liu Zhe-peng Liu gang Wei Wei-yue Lu School of Medical Instrument and Food Engineering University of Shanghai for Science and TechnologyShanghai 200093China Department of Pharmaceutics School of PharmacyFudan University&Key Laboratory of Smart Drug Delivery(Fudan University)Ministry of EducationShanghai 201203China

The authors regret that there was a picture error in *** Fig.8B,a copy mistake of H&E photographs was made when combining the images of different *** revisions do not affect any results and conclusions of the current *** original data of these figures have been provided to the Editorial Office,and the corresponding authors or the Editorial Office can be contacted for original data access.

关键词： application correction system

Endoscopy dissection of small stromal tumors emerged from the muscularis propria in the upper gastrointestinal tract:Preliminary study

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World Journal of Gastrointestinal Endoscopy 2012年第12期4卷 565-570页

作者： zhi-gang huang Xue-Song Zhang Shi-Liang huang Xiaogang Yuan Department of Gastroenterology Lihuili Hospital of Ningbo Medical Center

AIM:To investigate the feasibility and safety of the treatment of an upper gastrointestinal(GI) submucosal tumor with endoscopic submucosal dissection(ESD).METHODS:A total of 20 patients with esophageal and gastric submucosal tumors emerged from the muscular layer identified by endoscopic ultrasonography were collected from January 2009 to June *** or dumbbell-like lesions were excluded by an enhanced computerized tomography(CT) *** patients had intravenous anesthesia with propofol and then underwent the ESD procedure to resect these submucosal *** incision was closed by clips as much as possible to decrease complications,such as bleeding or perforation,after resection of the *** the specimens were collected and evaluated by hematoxylin,eosin and immunohistochemical staining,with antibodies against CD117,CD34,desmin,α-smooth muscle actin and vimentin to identify the characteristics of the ***'s criteria was used to evaluate the risk of gastrointestinal stromal tumors(GISTs).All patients underwent a follow-up endoscopy at 3,6 and 12 mo and CT scan at 6 and 12 ***:The study group consisted of 5 men and 15 women aged 45-73 years,with a mean age of 60.2 *** tumors were located in the esophagus,9 in the gastric corpus,4 in the gastric fundus,3 lesions in the gastric antrum and 1 in the gastric *** from the one case in the gastric angulus which was abandoned due to being deeply located in the serosa,94.7%(18/19) achieved complete gross dissection by ESD with operation duration of 60.52±30.32 *** average maximum diameter of tumor was 14.8±7.6 mm,with a range of 6 to 30 mm,and another lesion was ligated by an endoscopic ligator after most of the lesion was *** pathological and immunohistochemical analysis,12 tumors were identified as a GI stromal tumor and 6 were *** count of all 12 GIST lesions was fewer than 5 per 50 HPF and all lesions were at very low(9/12,75.0%) or low risk(3

关键词： Endoscopic submucosal dissection Stromal tumors Leiomyoma Upper gastrointestinal tract

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IL-23在炎症性肠病中的免疫调节作用

世界华人消化杂志 2009年第16期17卷 1649-1654页

作者：崔轶黄志刚刘占举同济大学附属第十人民医院胃肠内科上海市200072

IL-23属于IL-12家族,他可由IL-12P40亚单位和IL-23P19亚单位通过二硫键形成的异二聚体分子,通过结合T细胞表面上的受体复合物(IL-12Rb1和IL-23R构成)激活Stat1、Stat3及Stat4信号传导通路,诱导IL-10和INF-γ的产生.IL-23还可以诱导初始C... 详细信息

IL-23属于IL-12家族,他可由IL-12P40亚单位和IL-23P19亚单位通过二硫键形成的异二聚体分子,通过结合T细胞表面上的受体复合物(IL-12Rb1和IL-23R构成)激活Stat1、Stat3及Stat4信号传导通路,诱导IL-10和INF-γ的产生.IL-23还可以诱导初始CD4+T细胞分化为具有致病性的Th17细胞,并生成IL-17、IL-6和TNF-α,引起慢性结肠炎症的发生.研究发现,IL-23/IL-17轴在炎症性肠病的发病机制中可能处于关键地位,并有望成为新的治疗靶点.

关键词：炎症性肠病白介素一23 白介素一17 黏膜免疫受体

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中、美、英商标与域名争议解决办法比较研究

科技与法律 2007年第1期 63-67,9页

作者：黄智刚四川省人大常委会法制工作委员会二处

因特网的商业化和电子商务的迅猛发展使得商业机构在因特网上的存在非常重要。注册域名是实现这一目标的最有效办法之一,因为这样可以在商务活动中把商标识别与网络世界有机的结合起来。然而,域名的使用会产生法律和商业上的问题。其中... 详细信息

因特网的商业化和电子商务的迅猛发展使得商业机构在因特网上的存在非常重要。注册域名是实现这一目标的最有效办法之一,因为这样可以在商务活动中把商标识别与网络世界有机的结合起来。然而,域名的使用会产生法律和商业上的问题。其中之一是一方注册一域名,将域名卖给商标的合法拥有人而获利。目前,在缺乏一个被广泛接受的争端解决机制的情况下,域名争端的处理大体上具有地域性特点。然而,由于因特网的全球特性,域名争端已经超越了任意一个特定意义上的地理区域。本文比较了域名与商标争议在中、美、英的处理原则。这种比较重点集中在法规分析,以发现在这三种司法制度下解决方式的利弊。本文对今后的发展趋势进行了讨论并向中国的立法机关、商标和域名拥有者提出了相应的建议。

关键词：因特网商标域名

基于广义马尔科夫链模型的动柔度预测(英文)

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机床与液压 2014年第24期42卷 67-70页

作者：黄志刚谢锋云华东交通大学机电学院南昌330013

动柔度对机械加工的稳定性和精度有重要的影响。对动柔度进行预测能为机床加工精度补偿和稳定性分析提供实际的指导作用。由于不确定性的存在会降低结果的可靠性与可信度,传统的马尔科夫链预测方法没有考虑不确定性问题。为了提高预测... 详细信息

动柔度对机械加工的稳定性和精度有重要的影响。对动柔度进行预测能为机床加工精度补偿和稳定性分析提供实际的指导作用。由于不确定性的存在会降低结果的可靠性与可信度,传统的马尔科夫链预测方法没有考虑不确定性问题。为了提高预测结果的可靠性与可信度,在马尔科夫链模型的基础上提出了一个广义马尔科夫链模型,并通过广义马尔科夫链模型对动柔度进行了预测。结果表明:提出的预测方法有一个好的预测性能。

关键词：动柔度广义马尔科夫链广义区间预测