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A novel application of horseradish peroxidase:Oxidation of alcohol ethoxylate to alkylether carboxylic acid

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Chinese Chemical Letters 2008年第12期19卷 1411-1414页

作者： li Fei zhi Qiu xiao li Yun ling li China Research Institute of Daily Chemical Industry Taiyuan 030001 China

A novel application of horseradish peroxidase （HRP） in the oxidation of alcohol ethoxylate to alkylether carboxylic acid in the present of H2O2 was reported in this paper. We propose the mechanism for the catalytic oxidation reaction is that the hydrogen transfers from the substrate to the ferryl oxygen to form the α-hydroxy carbon radical intermediate. The reaction offers a new approach for further research structure and catalytic mechanism of HRP and production of alkylether carboxylic acid.

关键词： Horseradish peroxidase Alcohol ethoxylate Alkylether carboxylic acid

Altered Expression of Differential Genes in Thoracic Spinal Cord Involved in Experimental Cholestatic Itch Mouse Model

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Current Medical Science 2018年第4期38卷 679-683页

作者： Ming CHEN zhi-xiao li Qian WANG Hong-bing XIANG Department of Anesthesiology Hubei Maternal and Child Health Hospital Wuhan 430060 China Department of Anesthesiology and Pain Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

The spinal origin of cholestatic itch in experimental obstructive jaundice mouse model remains poorly understood. In this study, the jaundice model was established by bile duct ligation （BDL） in mice, and differential gene expression patterns were analyzed in the lower thoracic spinal cord involved in cholestatic pruritus after BDL operation using high-throughput RNA sequencing. At 21st day after BDL, the expression levels of ENSRNOG00000060523, ENSRNOG00000058405 and ENSRNOG00000055193 mRNA were significantly up-regulated, and those of ENSRNOG00000042197, ENSRNOG00000008478, ENSRNOG00000019607, ENSRNOG00000020647, ENSRNOG00000046289, Gemin8, Serpina3n and Trim63 mRNA were significantly down-regulated in BDL group. The RNAseq data of selected mRNAs were validated by RT-qPCR. The expression levels of ENSRNOG00000042197, ENSRNOG00000008478, ENSRNOG00000019607, ENSRNOG00000020647, ENSRNOG00000046289 and Serpina3n mRNA were significantly down-regulated in BDL group. This study suggested that cholestatic pruritus in experimental obstructive jaundice mouse model is related with in the changes of gene expression profiles in spinal cord.

关键词： cholestatic itch differential genes spinal cord high-throughput RNA sequencing

Mapping Changes of Whole Brain Blood Flow in Rats with Myocardial Ischemia/Reperfusion Injury Assessed by Positron Emission Tomography

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Current Medical Science 2019年第4期39卷 653-657页

作者： Xu-chu PAN zhi-xiao li Duo-zhi WU Shun-yuan li Hong-bing XIANG Yong-tang SONG Medical Association of Hubei Province Wuhan 430071China Department of Anesthesiology and Pain Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Anesthesiology Hainan General HospitalHaikou 570311China Department of Anesthesiology the First Affiliated Quanzhou Hospital of Fujian Medical UniversityQuanzhou 362000China

l8F-labeled fluorodeoxyglucose positron emission tomography (l8F-FDG PET) is the most sensitive tool for studying brain metabolism in *** investigated the image patterns of 18F-FDG PET during reperfusion injury and correlated changes of whole brain blood flow utilizing a rat myocardial ischemia/reperfiision injury (MIRI) *** results assessed by echocardiography indicated resultant cardiac dysfunction after ischemia-reperfusion in the rat *** was found that the average standardized uptake value (SUVaverage) of the whole brain was significantly decreased in model rats,and the glucose uptake of different brain regions including accumbens core/shell (Acb),left caudate putamen (LCPu),hippocampus (HIP),left hypothalamus (LHYP),olfactory (OLF),superior colliculus (SC),right midbrain (RMID),ventral tegmental area (VTA),inferior colliculus (IC) and left thalamus whole (LTHA) was significantly decreased in MIRI rats whereas no significant difference was found in the SUVaverage of amygdala (AMY),right Cpu,RHYP,right HYP,left MID,right THA,pons and medulla oblongata (MO).These l8F-FDG PET data provide a reliable identification method for brain metabolic changes in rats with MIRI.

关键词： cardiac ischemia-reperfusion injury brain metabolism 18-fluorodeoxyglucose positron emission tomography

Research advances in the study of Pistacia chinensis Bunge, a superior tree species for biomass energy

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Forestry Studies in China 2007年第2期9卷 164-168页

作者： li Hong-lin Zhang zhi-xiang lin Shan-zhi li xiao-xu College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 P. R. China

As a renewable energy, biomass energy has aroused wide attention and studies of this issue have become a hot topic throughout the world. Pistacia chinensis Bunge （Anacardiaceae） is a superior species for biomass energy with high oil content in seeds and wide geographic distribution. It is a dioeciously, deciduous arbor, flowering from March to April and bearing fruits from September to November. The classification, regional distribution and biological characteristics ofP chinensis are stated in this paper, then, research advances in the growth, breeding and physiology of this species are summarized. The problems in present studies are broached. Finally, a future direction for research is proposed.

关键词： Pistacia chinensis Bunge biomass energy of forest

Glucose Metabolic Alteration of Cerebral Cortical Subareas in Rats with Renal Ischemia/Reperfusion Based on Small-Animal Positron Emission Tomography

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Current Medical Science 2021年第5期41卷 961-965页

作者： Ming CHEN Mei ZHANG zhi-xiao li Hong-bing XIANG Jun XIONG Department of Anesthesiology Maternal and Child Health Hospital of Hubei ProvinceWuhan 430070China The Central Hospital of Wuhan Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430014China Department of Anesthesiology and Pain Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Hepatobiliary Surgery Center Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Objective:To investigate glucose metabolic alterations in cerebral cortical subareas using ^(18)F-labeled glucose derivative fluorodeoxyglucose(FDG)micro-positron emission tomography(PET)scanning in a rat renal ischemia/reperfusion(RIR)***:Small-animal PET imaging in vivo was performed with ^(18)F-labeled FDG as a PET tracer to identify glucose metabolic alterations in cerebral cortical subregions using a rat model of ***:We found that the average standardized uptake value(SUV_(average))of the cerebral cortical subareas in the RIR group was significantly increased compared to the sham group(Plic machinery of cerebral cortex subregions.

关键词： renal ischemia/reperfusion cerebral cortex glucose uptake ^(18)F-fluorodeoxyglucose positron emission tomography

Beyond 1,4-addition of in-situ generated(aza-)quinone methides and indole imine methides

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Chinese Chemical Letters 2024年第7期35卷 35-53页

作者： Yan-li li zhi-Ming li Kai-Kai Wang xiao-Long He Medical College Xinxiang UniversityXinxiang 453000China School of Pharmacy Xinxiang UniversityXinxiang 453000China School of Food and Bioengineering Xihua UniversityChengdu 610039China

The conjugate addition of in-situ generated(aza-)quinone methides(QMs)and indole imine methides(IIMs)emerged as a powerful protocol to access densely functionalized benzenes and *** alcohols,aminobenzhydryl alcohols,and varied indolylmethanols served as most effective precursors for the in-situ generation of such reactive species under acid *** relevant propargylic alcohol has proven to be an elegant precursor to generate the propargylic-QMs and-IIMs via the acid promoted dehydration process,thus enabling diverse challenging remote activation to proceed conjugate1,6-and 1,***,the heteroarene has proven to be workable to transfer the LUMO of the p-QMs and 2-IIMs,thus inducing the remote nucleophilic dearomative *** conjugate additions of(aza-)p-QMs and varied IIMs has made significant contribution in the field of remote activation chemistry in past *** review summarizes the latest advances of the remote conjugate additions of the in-situ generated QMs and IIMs.

关键词： Conjugate addition QMs IIMs LUMO Remote activation Dearomative addition

Progress in processing of porous titanium:a review

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Rare Metals 2024年第5期43卷 1932-1955页

作者： xiao-li Yang Xian-Feng Du zhi-lin Xu Zhong-Shuai liang li-Long Xiong Engineering Research Center of Energy Storage Materials and Devices Ministry of EducationSchool of ChemistryXi'an Jiaotong UniversityXi'an 710049China

As a novel structural and functional material,porous titanium and its alloys have been widely used in the aerospace,marine engineering and biomedical fields due to their high corrosion resistance,low density,good biocompatibility and excellent mechanical ***,in this paper,a comprehensive review of powder metallurgy(PM)(including additive manufacturing(AM)processes)for fabricating porous titanium is firstly covered in terms of their working principles,capabilities,shortcomings and ***,the influencing factors of various methods on final pore structure of porous Ti are ***,a summary of the chemical methods(CM)to obtain the porous Ti is also provided,such as dealloying method and reduction ***,the tendency and direction of preparation technology as well as application of porous titanium were prospected.

关键词： Porous titanium Powder metallurgy Chemical method Pore structure Processing process

Effect of stress-relief annealing on thermal fatigue cracks for die-casting die steels

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Journal of Iron and Steel Research International 2024年第5期31卷 1164-1176页

作者： zhi-xiong Bai xiao-chun Wu School of Materials Science and Engineering Shanghai UniversityShanghai200444China

The effect of stress-relief annealing at different stages of thermal fatigue tests on crack growth was investigated using a self-built thermal cycle *** results showed that annealing could limit crack expansion *** reduced dislocation density and released the accumulation of residual *** addition,the strain accumulation in carbides was reduced by this *** was also found that double annealing was even more effective at inhibiting crack expansion compared to single stress-relief *** 1000 cycles,the maximum crack length was reduced by 31.1%and 45.2%for the samples after using the optimal single and double annealing processes,*** single stress-relief annealing,earlier annealing provides more benefit in delaying crack ***,effective double stress-relief annealing requires a suitable time interval between the annealing ***,after 800 cycles,surface hardness decreased significantly accompanied by an increase in the size and number of carbides,and cracks expanded predominantly along grain boundaries.

关键词： Thermal fatigue Steel Carbide Stress-relief annealing Dislocation Residual stress

Genetic Analysis and Molecular Mapping of light-Sensitive Red-Root Mutant in Rice

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Rice science 2009年第1期16卷 27-32页

作者： ZHANG Jun-zhi liU xiao li Chao xiao Ke DONG Yan-jun Laboratory of Plant Genetics and Functional Genes College of Life and Environmental Sciences Shanghai Normal University Shanghai 200234 China

The light-sensitive red-root mutant, designated as HG1, was newly observed from an indica rice variety, Nankinkodo, when seedlings were grown with roots exposed to natural light. The root color of the mutant began to turn slight-red when the roots were exposed to the light at the intensity of 29 ）Jmol/（m^2·s）, then turned dark-red at the light intensity of 180 pmol/（m^2·s）, suggesting that the root color of the mutant was evidently sensitive to light. Furthermore, genetic analysis showed that the character of light-sensitive red-root of the HG1 mutant was controlled by a single dominant gene, tentatively designated as Lsr. With simple sequence repeat markers, Lsrgene was located between the markers RM252 and RM303 on chromosome 4 with the genetic distances of 9.8 cM and 6.4 cM, respectively. These results could be useful for fine mapping and cloning of Lsrgene in rice.

关键词： rice light sensitivity red root mutant genetic analysis gene mapping